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Chen Xuefeng,Chen Wentao,Zhang Yifu,Peng Bin,Wang Yuling,Xu Mingwei 한국화학공학회 2024 Korean Journal of Chemical Engineering Vol.41 No.1
Oil and gas pipeline accidents not only caused personnel casualties, economic losses and environmental pollution but also brought great panic to the public. To provide accurate accident prevention strategies, risk assessment (RA) has been widely used in the oil and gas pipeline industry. It aims to quantify the potential risk due to unexpected eventualities. However, the current RA methods mainly pay attention to the tangible risk (such as the human safety risk, economic risk and environmental risk), and the intangible risk (such as reputation loss risk) is often neglected. This cannot refl ect the real risk for the oil and gas pipeline. To settle existing research limitation, a two-dimensional risk assessment method for oil and gas pipelines based on “accident probability–reputation loss” is proposed. The method mainly involves four steps, namely, risk identifi cation, probability calculation, consequence assessment and risk assessment. The proposed method is used to quantitatively assess a natural gas pipeline in central China. Its results indicate that pipeline risk is unacceptable due to the unacceptability of accident probability and various dimensions of risk. Therefore, this study can enrich and develop the current oil and gas pipeline RA method to refl ect the real risk of oil and gas pipeline accidents.
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Marein Prevented LPS-Induced Osteoclastogenesis by Regulating the NF-κB Pathway In Vitro
( Yuling Li ),( Jing Zhang ),( Caiping Yan ),( Qian Chen ),( Chao Xiang ),( Qingyan Zhang ),( Xingkuan Wang ),( Ke Jiang ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.2
Many bone diseases such as osteolysis, osteomyelitis, and septic arthritis are caused by gramnegative bacterial infection, and lipopolysaccharide (LPS), a bacterial product, plays an essential role in this process. Drugs that inhibit LPS-induced osteoclastogenesis are urgently needed to prevent bone destruction in infective bone diseases. Marein, a major bioactive compound of Coreopsis tinctoria, possesses anti-oxidative, anti-inflammatory, anti-hypertensive, antihyperlipidemic, and anti-diabetic effects. In this study, we measured the effect of marein on RAW264.7 cells by CCK-8 assay and used TRAP staining to determine osteoclastogenesis. The levels of osteoclast-related genes and NF-κB-related proteins were then analyzed by western blot, and the levels of pro-inflammatory cytokines were quantified by ELISA. Our results showed that marein inhibited LPS-induced osteoclast formation by osteoclast precursor RAW264.7 cells. The effect of marein was related to its inhibitory function on expressions of pro-inflammatory cytokines and osteoclast-related genes containing RANK, TRAF6, MMP-9, CK, and CAII. Additionally, marein leads to markedly inhibited NF-κB signaling pathway activation in LPS-induced RAW264.7 cells. Concurrently, when the NF-κB signaling pathway was inhibited, osteoclast formation and proinflammatory cytokine expression were decreased. Collectively, marein could inhibit LPS-induced osteoclast formation in RAW264.7 cells via regulating the NF-κB signaling pathway. Our data demonstrate that marein might be a potential drug for bacteria-induced bone destruction disease. Our findings provide new insights into LPS-induced bone disease.
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An Improved Coverless Text Steganography Algorithm Based on Pretreatment and POS
( Yuling Liu ),( Jiao Wu ),( Xianyi Chen ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.4
Steganography is a current hot research topic in the area of information security and privacy protection. However, most previous steganography methods are not effective against steganalysis and attacks because they are usually carried out by modifying covers. In this paper, we propose an improved coverless text steganography algorithm based on pretreatment and Part of Speech (POS), in which, Chinese character components are used as the locating marks, then the POS is used to hide the number of keywords, the retrieval of stego-texts is optimized by pretreatment finally. The experiment is verified that our algorithm performs well in terms of embedding capacity, the embedding success rate, and extracting accuracy, with appropriate lengths of locating marks and the large scale of the text database.
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Yuling Qiu,Yali Chen,Haiyang Yu,Qianxiang Zhou,Ran Wang,Meihua Jin,Dexin Kong 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
Bee venom has been used for treating various diseases for a long time. However, the bioactive constituents of bee venom and its mechanisms remain poorly understood. In the present study, phospholipase A2 (Bt-PLA2) cDNA was cloned, and a mature form of Bt-PLA2 was purified from bumblebee venom (Bombus terrestris). The differentiation induction and apoptosis induction activities of Bt-PLA2 on chronic myelogenous leukemia (CML) K562 cells were also evaluated. Bt-PLA2 cDNA has 540 nucleotides that encode a 180-amino-acid protein. The purified, mature form of Bt-PLA2 was an 18-kDa protein, and it inhibited K562 cell growth, determined by an IC50 value of 29.5 ng/μl. Moreover, Bt-PLA2 induced erythroid differentiation of K562 cells in a dose-dependent manner, and this was supplemented with the upregulation of glycophorin A (GPA) mRNA expression. Bt-PLA2- induced apoptosis, analyzed by DAPI staining, was correlated with the result analyzed by AnnexinV-FITC/PI binding. Furthermore, activation of caspase 3 and poly ADP-ribose polymerase (PARP) and inhibition of p-Akt, determined by western blot, further demonstrated that Bt-PLA2 induced apoptosis mainly through the Akt pathway. The parallel induction of erythroblasts differentiation of K562 cells and apoptosis due to Bt-PLA2 treatment demonstrated the potential use of Bt-PLA2 as an anti-leukemia drug lead.
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An Improved Coverless Text Steganography Algorithm Based on Pretreatment and POS
Liu, Yuling,Wu, Jiao,Chen, Xianyi Korean Society for Internet Information 2020 KSII Transactions on Internet and Information Syst Vol.14 No.4
Steganography is a current hot research topic in the area of information security and privacy protection. However, most previous steganography methods are not effective against steganalysis and attacks because they are usually carried out by modifying covers. In this paper, we propose an improved coverless text steganography algorithm based on pretreatment and Part of Speech (POS), in which, Chinese character components are used as the locating marks, then the POS is used to hide the number of keywords, the retrieval of stego-texts is optimized by pretreatment finally. The experiment is verified that our algorithm performs well in terms of embedding capacity, the embedding success rate, and extracting accuracy, with appropriate lengths of locating marks and the large scale of the text database.
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鄭玉玲,呼和,진가유 한국알타이학회 2002 알타이학보 Vol.0 No.12
There have been many controversies concerning the nature and placement of Mongolian stress. Any experimental phonetic research into Mongolian stress has not been done yet. This paper aims to discuss phonetic properties and placement of Mongolian stress in trisyllabic words by conducting an acoustic analysis and a perception test. The most important perceptual cue of Mongolian stress was proved to be vowel length. When the first syllable has a long vowel (i.e. long-long-long, long-long-short, long-short-long, long-short-short), this syllable was perceived as being stressed. When the first syllable has a short syllable (i.e. short-short-short, short-short-long, short-long-short, short-long-ling), the second syllable was perceived as being stressed.
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Modulation of the Self-Assembly of Collagen by Phytic Acid: An In Vitro Study
Xiao Tu,Xincheng Chen,Ying Peng,Jie Nan,Benmei Wei,Lang He,Chengzhi Xu,Yuling Xu,Dong Xie,Juntao Zhang,Haibo Wang 한국고분자학회 2018 Macromolecular Research Vol.26 No.13
Phytic acid, containing a myoinositol ring coupled with six phosphate groups, can react with the amino groups of collagen to regulate their self-assembly behavior. The aim of this research is to evaluate the effects of phytic acid on the selfassembly behavior of collagen, the structures and properties of the resulting fibrils and hydrogels. Turbidity and chloramine T assay suggested that phytic acid could improve the self-assembly kinetics and degree of collagen, and the optimal ratio of phytic acid/collagen was 1/1 (w/w). Scanning electron microscopy (SEM) analysis indicated that co-fibrils of collagen with phytic acid are more slender than that of pure collagen, and transmission electron microscopy (TEM) reveals that the characteristic D-periodicity of collagen fibrils is not affected by phytic acid. Besides, differential scanning calorimetry (DSC) and rheology revealed that the thermal stability of collagen fibrils and the viscoelasticity of collagen hydrogels could be improved by phytic acid and the optimal ratio of phytic acid/collagen is 1/1 (w/w).
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Ziwuliuzhu Acupuncture Modulates Clock mRNA, Bmal1 mRNA and Melatonin in Insomnia Rats
Huang Ao,Xiao Gefang,Chen Yiliu,Hu Zuying,Lee Pin-Hsuan,Huang Yusen,Zhuang Zifeng,Zhang Yuling,Qing Peng,Zhao Canghuan 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.3
Background: In clinics, Ziwuliuzhu acupuncture is widely considered an effective method of treating insomnia; however, there is currently limited information available regarding its possible mechanisms. Although the method of Ziwuliuzhu acupuncture possesses a unique rhythmic pattern. Objectives: In this study, we have creatively combined the traditional Chinese medicine of Ziwuliuzhu with a modern biological rhythm to investigate the internal mechanism of insomnia. Methods: Pathological tissue from the hypothalamus was analyzed using hematoxylin– eosin staining. The level of TNF (tumor necrosis factor)-α in the SCN (suprachiasmatic nucleus) area of the hypothalamus was detected in situ using the TUNEL fluorescence staining assay. The concentration of hypothalamic melatonin was detected using the enzyme-linked immunosorbent assay (ELISA). The mRNA expression of Clock and Bmal1 was measured using RT-qPCR. Results: In the Ziwuliuzhu acupuncture groups, the structural damage in the hypothalamic neurons was alleviated compared to the model group and the expression of inflammatory factors was reduced. The mRNA expression levels of Clock and Bmal1 were significantly increased (p < 0.05). The concentration of melatonin was significantly increased (p < 0.001). Although there were no significant differences between the treatment groups (diazepam group, Nazi group, Najia group, and routine group) (p > 0.05). Conclusion: Ziwuliuzhu acupuncture alleviated neuronal damage and modulated the inflammatory reaction in the hypothalamus of rats with insomnia. Moreover, Ziwuliuzhu acupuncture increased the expression levels of Clock and Bmal1 mRNA, and MT content. This study has potentially highlighted one of
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