일부 고지혈증 폐경 여성의 Isoflavone 공급에 따른 혈중지질 변화에 관한 연구

이다홍,승정자,이행신,김미현,서유리 대한지역사회영양학회 2001 대한지역사회영양학회지 Vol.6 No.1

Intake of soy protein may decrease the risk factors associated with cardiovascular disease in postmenopausal women. This study was designed to investigate the effects of isoflavone supplementation on serum lipids in 16 hyperlipidemic postmenopausal women. For this purpose, an intervention study was conducted for 12 weeks. Subjects were healthy, free-living women consuming habitual diets with 0.3 g/d of isoflavone. Food and nutrient intake was obtained by 24-hr recall method and anthropometric measurements were made. Systolic and diastolic blood pressure, total serum cholesterol, HDL-cholesterol and LDL-cholesterol were determined before and after the isoflavone supplementation. The results were summarized as follows. The average age, height, weight and BMI of the subjects were 65.3 years, 151.4 cm, 62.2 kg and 27.1, respectively. The systolic blood pressure and diastolic blood pressure were not reduced significantly with isoflavone supplementation. Total cholesterol (p < 0.001), HDL-C (p < 0.05), and LDL-C (p < 0.01) were significantly increased after isoflavone concentration. In conclusion, isoflavone supplementation was not effective to modify risk factors for cardiovascular disease.

특수가공용 공작기계의 쇼트피닝기 모델설계에 관한 연구

신용승,이승호,서유원,정성교 서울産業大學校 1999 논문집 Vol.49 No.2

As developing the technology in industry, the materials with superior grade of stiffiness and lightness are required, and hence the higher fatigue strength characteristics are necessary. Thus, the non-traditional machining to increase fatigue strength is becoming influential as very important technology. Considering environmental problem, the shot peening process is selected as a good process to increase fatigue strength. But the data related to shot peening process are not enough in this country. Shot peening process is essential to ensure repeatability and uniformity on a part to part and lot to lot basis, while conforming to the applicable specifications. The shot material, size, shape and hardness as well as velocity and impact angle must be controlled to provide consistency in peening results. In order to effectively control the shot peening process, parameters must be addressed in the following order, such as media control, coverage control, intensity control, equipment control and so on.

기계적 자극이 치주인대 세포의 osteoprotegerin과 receptor activator of nuclear factor κB ligand mRNA 발현에 미치는 영향

이기주,이승일,황충주,,옥승호,전옥순 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.4

본 연구는 치주인대 세포에 지속적이고 점진적 인장력을 가하여 치아 이동 시 형성되는 인장부위의 기계적 자극에 대한 생화학적 전달과 치조골 흡수와 생성 조절 기전을 이해하고자 하였다. 치주인대 세포가 배양된 유연한 성장 표면을 가진 배지에 지속적이고 점진적인 인장력을 가하고 골흡수 인자인 PGE_(2)와 골형성 인자인 ALP의 생성량을 1, 3, 6, 12시간 후에 측정하여 정량비교하였고 파골세포 분화기전을 조절하는 OPG, RANKL의 인자들과 matrix metalloproteinase (MMP)-1, -8, -9, -13, tissue inhlbitor of matrix metalloproteinase (TIMP)-1의 인자들을 역전사 중합효소 연쇄반응 검사하여 m-RNA 발현을 비교한 결과, 치주인대 세포에 인장력을 가한 경우 대조군보다 PGE_(2)의 농도가 적었고 (p<0.05) ALP의 농도 변화는 없었으며 OPG의 mRNA 발현이 증가하였으나, RANKL의 mRNA 발현은 감소하였다. 그리고 TIMP-1과 MMP-1, -8, -9, -13의 mRNA 발현이 대조군과 차이가 없었다. 이상의 연구에서 사람의 치주인대 세포는 점진적이고 지속적인 인장력에 대한 반응으로 PGE_(2)의 생성과 RANKL의 mRNA 발현은 감소하고 OPG의 mRNA 발현은 증가하여 골흡수를 억제하는 효과를 보이는 것으로 나타났다. Tooth movement is a result of mutual physiologic responses between the periodontal ligament and alveolar bone stimulated by mechanical strain. The PDL cell and osteoblast are known to have an influence on bone formation by controlling collagen synthesis and alkaline phosphatase activation. Moreover, recent studies have shown that the PDL cell and osteoblast release osteoprotegerin(OPG) and the receptor activator of nuclear factor κB ligand (RANKL) to control the level of osteoclast differentiation and activation which in turn influences bone resorption. In this study, progressively increased, continuous tenslonal force was applied to PDL cells. The objective was to progressively increased. continuous tensional force was applied to PDL cells. The objective was to find out which kind of biochemical reactions occur after tensional force application and to illuminate the alveolar bone resorption and apposition mechanism. Continuous and progressively increased tensile force was applied to PDL cells cultured on a petriperm dish wlth a flexible membrane. The amount of PGE_(2) and ALP synthesis were measured after 1, 3, 6 and 12 hours of force application. Secondly, RT-PCR analysis was carried out for OPG and RANKL whlch control osteoclast differentiation and MMP-1, -8, -9, -13 and TIMP-1 which regulate the resolution of collagen and resorption of the osteoid layer. According to the results, we concluded that progressively increased, continuous force application to human PDL cells reduces PGE_(2) synthesls, and increases OPG mRNA expression.

다양한 교정용 브라켓 원재료에 부착하는 mutans streptococci 양의 비교분석

유진경,안석준,이신재,장영일 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.2

Objective: To estimate the effects of bracket material type on enamel decalcification during orthodontic treatment, this study analyzed the adhesion level of mutans streptococci (MS) to orthodontic bracket materials in vivo. Methods: Three different types of orthodontic bracket materials were used: stainless steel, monocrystalline sapphire, and polycrystalline alumina. A balanced complete block design was used to exclude the effect of positional variation of bracket materials in the oral cavity. Three types of plastic individual trays were made and one subject placed the tray in the mouth for 12 hours. Then, the attached bacteria were isolated and incubated on a mitis salivarius media containing bacitracin for 48 hours. Finally, the number of colony forming units of MS was counted. The experiments were independently performed 5 times with each of the 3 trays, resulting in a total of 15 times. Mixed model ANOVA was used to compare the adhesion amount of MS. Results: There was no difference in colony forming units among the bracket materials irrespective of jaw and tooth position. Conclusions: This study suggested that the result of quantitative analysis of MS adhesion to various orthodontic bracket materials in vivo may differ from that of the condition in vitro. 법랑질 탈회는 교정 치료 중에 발생하는 대표적인 부작용으로서 특히 교정용 브라켓에 대한 세균 부착이 그 원인이 될 수 있다. 본 연구의 목적은 서로 다른 표면 특성을 가진 세 종류의 브라켓을 구강 내 장착했을 때 각 재료의 표면에 대한 mutans streptococci 부착 정도를 측정하여 브라켓 재료에 따른 법랑질 탈회 및 치아 우식 발생 가능성을 분석하는 것이었다. 상하악 및 치아 부위별 mutans streptococci 부착 정도의 차이를 배제하고 브라켓 원재료에 따른 차이만을 검출하기 위하여 균형완전블록 실험계획을 설계하였다. 피실험자인 30세 여성의 구강 내에 장착할 수 있는 tooth positioner 형태로 3세트의 플라스틱 맞춤 트레이를 제작하였으며, 이 트레이에 직육면체의 형태(4 x 3 x 2 mm)로 제작한 stainless steel, monocrystalline sapphire, polycrystalline alumina 조각을 세트마다 서로 다른 순서로 전치부와 구치부 순면에 부착하였다. 이렇게 제작된 3종류의 실험장치를 12시간 동안 피실험자의 구강 내에 장착한 후, 각 브라켓 재료 표면에 형성된 치태를 채취하여 bacitracin이 포함된 mitis salivarius배지에서 48시간 배양 후 colony counting을 통해 그 표면에 부착된 mutans streptococci 양을 비교하였다. 이와 같은 방법으로 3세트의 실험장치에 대해서 각각 5회씩 총 15회의 실험을 시행하였다. 그 결과 상하악 및 치아 부위별 및 브라켓 재료별 세균 부착 정도는 모두 유의한 차이를 보이지 않았다. 결론적으로 본 연구의 결과 in vivo condition에서 브라켓 재료의 차이는 mutans streptococci 부착에 영향을 미치지 못하는 것으로 보였다.

뇌졸중 노인을 돌보는 주간호제공자의 부담감과 예측요인 : 한방병원 입원 노인을 중심으로

유수정,김신미,이윤정,박연환 노인간호학회 2004 노인간호학회지 Vol.6 No.1

Purpose : The purpose of this study was to investigate factors influencing burden in primary caregivers caring for an elderly person who has had a stroke. Method : A convenience sample of 124 primary caregivers of elderly people with a stroke admitted to an oriental medicine hospital participated in this study. Interviews were done with a standardized questionnaire including Caregiver Burden Scale (Chang, 1995). Result: Average burden score was 93.19, indicating a moderate to severe level of burden. Time-dependent burden had the highest score of the sub-domains. For general characteristics, the factors significantly influencing caregiver burden were gender and age of the elderly person, age and educational level of the caregiver, whether living together or not, and the presence of another caregiver. Multiple regression analyses demonstrated that level of ADL of the elderly person, relationship between the elderly person and the caregiver prior to the stroke, caregivers' relation to the elderly person and primary income were significant factors in explaining variance in overall burden. Conclusion : Recognition of high levels of caregiver burden and factors influencing burden will allow us to develop different nursing strategies to decrease caregiver burden in those caring for an elderly person with a stroke.

수열합성법에 의한 제조된 MCM-41에 담지된 4급암모늄염 촉매를 이용한 페녹시메틸에틸렌 카보네이트의 합성

신동환,유병수,이화수,박대원 한국공업화학회 2003 응용화학 Vol.7 No.1

In the present study, the synthesis of phenoxy methyl ethylene carbonate (PMEC) from carbon dioxide and phenyl glycidyl ether(PGE) was investingated using mesoporous chloropropyl-methyl-MCM-41-supported quaternary ammonium salt catalysts. The influence of the amounl of modifier and quaternary ammonium salt structure on the conversion of PGE was discussed. The MCM-41-immobilized quaternary ammonium salts showed good catalytic activity even at the atmospheric pressure of carbon dioxide, and it could be reused with easy seperation.

다당류를 이용한 생분해성 필름의 제조

신은경,김동운,김유은,나건,이기영 全南大學校 觸媒硏究所 1998 觸媒硏究 論文集 Vol.20 No.-

In this study, we prepared biodegradable polysaccharide films with pullulan, κ-carrageenan, curdlan and chitosan and measured physical properties and antibacterial activity All of polysaccharide films showed the high biodegradability but antibacterial activity on bacteria was only observed at chitosan film. The permeability of the gas(O₂and C0₂) of polysaccharide films besides chitosan film showed 50∼100 times lower than polyethylene(PE) film. Therefore, we prepared two film types to give low gas permeability and antibacterial property at polysaccharide film : one type was the physically mixed film(k-carrageenan +chitosan) and other type was the chitosan coated κ-carrageenan film. Chitosan coated κ-carrageenan film showed low gas permeability and antibacterial activity.

자일리톨과 불화나트륨을 함유한 저작성 정제가 교정환자의 구강위생에 미치는 영향에 관한 임상시험

유상훈,안석준,이신재,백승학,김태우,장영일,남동석 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.2

본 연구는 불화나트륨과 자일리톨 성분으로 구성된 구강 청정용 저작성 정제(덴포정, 하미즐, 부천, 경기)가 교정치료 중인 환자의 구강위생 상태에 미치는 영향을 알아보기 위해 시행되었다. 교정치료 중인 18세 이상의 성인 환자 30명을 대상으로 약제 상요 전, 2주 후, 4주 후에 피험자의 상악과 하악 전치 부위 및 구치 부위에서 각각 치은 지수, 치은 출혈 지수, 치태 지수를 측정하여 다음과 같은 결과를 얻었다. 1. 상하악 전치부와 구치부 모두에서 치태 지수가 약제 사용 전에 비해 사용 2주 후와 4주 후 유의성 있게 감소하였다(p<0.05). 또한, 적용 2주 후와 4주 후의 지수를 비교하였을 때 4주 후에 좀 더 개선된 경향을 보였으나, 통계적 유의성은 나타나지 않았다(p<0.05). 2. 치은 지수, 치은 출혈 지수도 상하악 전치부와 구치부 모두에서 적용 전에 비하여 2주 후와 4주 후 유의성 있게 감소하였다(p<0.05). 적용 2주 후와 4주 후의 지수의 비교 검증에서 통계학적으로 유의할 만한 차이가 보이지 않아 구강위생 상태의 개선이 계속 유지됨을 보였다. 본 연구의 결과는 치태 지수, 치은 지수, 치은 출혈 지수가 실험 정제 적용 후에 감소하여 구강위생 상태가 개선되었음을 보였다. 이러한 결과를 통해 덴포정을 교정치료 중인 환자에서 부가적으로 사용하였을 경우 치주조직의 건강을 증진시키는데 도움을 줄 수 있으리라고 생각된다. The aim of this study was to evaluate the effect a chewable tablet containing sodium fluoride and xylitol (DenPo, Hamizzle, Bucheon, Kyungki) on the oral hygiene state in the orthodontic patients. The subjects consisted of 30 adult orthodontic patients, who have worn the fixed orthodontic appliance at least 6 months. They were instructed to use the tablet forth a day. Gingival index (GI), plaque index (PI), and sulcus bleeding index (SBI) of upper and lower anterior and posterior regions were measured before using the tablet (TO), 2 weeks after using the tablet (T1), and 4 weeks after using the tablet (T2). The data were analyzed with Wilcoxon signed ranks tests. The results were as follows ; 1. PI was decreased significantly in the T1 and T2 stage compared with TO stage in the both anterior and posterior regions (p<0.05). Although PI was decreased in the T2 stage compared with T1, there was not statistical significance(p>0.05). 2. GI and SBI showed similar results compared with PL. In the T1 and T2 stage, there was statistically significant decrease in the GI and SBI (p<0.05). This study showed that the DenPo tablet could be used as an adjunct to improve oral hygiene in the orthodontic patients. The decrease of the indices may suggest that DenPo tablet could be effective in the plaque removal and prevention of gingival inflammation in the orthodontic patients.

나노크기 은촉매를 이용한 에틸렌 산화반응

이상량,정유성,신재순,김영철,박남국 한국공업화학회 2002 응용화학 Vol.6 No.2

Partial oxidation of ethylene to ethylene oxide was studied using silver caralystsiadeposited on a low surface area support, such as d-alum(< 1 m^2/g). Theselectivity to ethylene oxide has been reported to be related to the surface area ofactive silver species. Thus the activity of silver catalysts was studied in view of theparticle size of Ag.The catalyst prepared by water-alcihol method showed higheractivity. The addition of Cs as a promoter at higher temperature was observed toincrease catalytic activity of silver catalysts, presumably by increasing the surfaceares of active silver.

비병원성 야생효모, Apiotrichum sacrabaeorum WP49-1로부터 β-Glucosidase 생산

신유리, 장지은, 이종수 배재대학교 자연과학연구소 2016 自然科學論文集 Vol.32 No.1

콩에 함유되어 있는 식물성 여성 호르몬인 estrogen의 흡수를 증가시키기 위해 높은 활성의 β-glucosidase를 생산하는 비병원성 야생 효모의 선별과 β-glucosidase의 생산 조건을 조사하였다. 38종의 비병원성 야생효모 중 Apiotrichum scarabaeorum WP49-1의 무세포 추출물이 6.5 unit의 가장 높은 β-glucosidase 활성을 보였고 Zygosaccharomyces rouxii O-5-7도 5.9 Unit의 높은 활성을 보였다. 최종적으로 β-glucosidase 생성 효모 균주로 Apiotrichum scarabaeorum WP49-1 균주를 선별하였다. β-Glucosidase는 Apiotrichum scarabaeorum WP49-1을 yeast extract-peptone-dextrose 배지에 접종하여 30℃에서 72시간 배양했을 때 최대로 생성되었다. 선발 균주는 구형으로 출아에 의한 영양증식을 하였고 포자를 형성하지 않았다. 또한 비타민이 함유되어 있지 않은 배지에서도 생육하였고 5% 에탄올에 대하여 내성을 보였다