Efficacy of Long-Term Tenofovir-Based Therapy in Chronic Hepatitis B Patients with Previous Nucleos(t)ide Analogues Treatment Experience

( Na Eun Lee ),( Hong Seon Son ),( Sung Hoon Choi ),( Chang Hun Lee ),( Seung Young Seo ),( Seong Hun Kim ),( Sang Wook Kim ),( Seung Ok Lee ),( Soo Teik Lee ),( Dae Ghon Kim ),( In Hee Kim ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Tenofovir disoproxil fumarate (TDF) is considered as the preferred treatment option for chronic hepatitis B (CHB) patients with treatment failure or resistance to prior nucleos(t)ide analogues (NAs) treatment. We investigated the efficacy of long-term TDF-based therapy in CHB patients with previous NAs-experience. Methods: This study included total 251 patients who had previous history of NAs therapy and were treated with TDF mono (n=173) or TDF combined with other NA (n=78) from August 2012 to March 2017. Virologic response (VR) was defined as undetectable serum HBV DNA by PCR (<20 IU/mL). Results: Mean age of patients was 49.3 years, median duration of TDF therapy was 27.2 months, 75.7% were HBeAg-positive, and median HBV DNA was 3.7 log10IU/mL. The cumulative rates of VR were 188/244 (77.0%), 180/211 (85.3%), and 146/161 (90.7%) at 12, 24, and 36 months, respectively. Multivariate analysis showed that body mass index (OR 0.77, 95% CI 0.61-0.95, p=0.0189) and duration of TDF therapy (OR 1.09, 95% CI 1.02-1.18, p=0.0221) was significantly associated with VR. TDF monotherapy, HBeAg-positivity, platelet count, serum albumin was associated with VR in the univariate analysis, but not significant in the multivariate analysis. In relation to renal safety, patients showed renal impairment (7, 3.0%), mild hypophosphatemia (15, 7.2%), severe hypophosphatemia (1, 0.4%). Conclusions: Long-term TDF-based therapy demonstrated highly effective in viral suppression and relatively favorable renal safety in CHB patients with previous NA-experience. The body mass index and duration of TDF therapy was independent factors associated with VR.

Piezoelectric Properties of Lead-Free Na_0.5K_0.5NbO₃ Ceramics Co-Doped with NiO and CuO

Tai, Weon-Pil,Kim, Young-Hyeok,Ok, Yun-Po,Ji, Han-Na,Kang, Sang-Kyu,Lee, Jae-Shin Informa UK (Taylor Francis) 2010 Integrated ferroelectrics Vol.114 No.1

Comparison of Virologic Response and Renal Safety of Long-Term Antiviral Therapy with Tenofovir and Entecavir in Naive Patient with Chronic Hepatitis B

( Hong Seon Son ),( Sung Hoon Choi ),( Na Eun Lee ),( Chang Hun Lee ),( Seung Young Seo ),( Seong Hun Kim ),( Sang Wook Kim ),( Seung Ok Lee ),( Soo Teik Lee ),( Dae Ghon Kim ),( In Hee Kim ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Tenofovir (TDF) entecavir (ETV) are considered as the preferred treatment options for treatment-naïve chronic hepatitis B (CHB) patients. We compared the virologic response and renal safety of long-term TDF versus ETV therapy in naïve CHB patients. Methods: This retrospective study included total 432 patients who were treated with TDF (n=205) or ETV (n=227) from August 2012 to March 2017. Virologic response (VR) was defined as undetectable serum HBV DNA by PCR (<20 IU/mL). Results: Mean age of patients, sex, baseline serum levels of AST, ALT, creatinine, and HBV DNA were not significantly different between TDF and ETV groups. The cumulative rates of VR between TDF and ETV groups were 71.6% vs. 61.8% (p=0.477), 88.1% vs. 79.6% (p=0.058), and 84.8% vs. 83.7% (p=0.966) at 1, 2, and 3 years, respectively. The cumulative rates of genotypic resistance between TDF and ETV groups were 0% vs. 0%, 0% vs. 1.5% (p=0.404), and 0% vs. 2.2% (p=0.447) at 1, 2, and 3 years, respectively. Incidences of renal impairment and hypophosphatemia during treatment up to 3 years were not significantly different between two groups. Multivariate analysis showed that HBeAg-positivity (OR 0.27, 95% CI 0.12-0.58, p=0.0012) and serum HBV DNA at 1 year 2000 IU/mL (OR 0.09, 95% CI 0.03-0.25, p<0.0001) were significantly associated with VR. Conclusions: Long-term TDF and ETV treatments appear to have similar virologic response and renal safety in naïve CHB patient. However, long-term ETV therapy might to be associated with genotypic resistance in 2.2% up to 3 years, while none of patients on TDF therapy did.

보육교사의 심리적 소진과 교사-영유아 상호작용의 관계에서 정서노동의 매개효과

이나영(Lee, Na-Young),국지윤(Kook, Ji-Youn),김영옥(Kim, Young-Ok) 한국영유아보육학회 2014 한국영유아보육학 Vol.0 No.89

본 연구는 보육교사의 심리적 소진과 교사-영유아 상호작용의 관계에서 정서노동이 어떠한 영향을 미치는지 살펴보고자 하였다. 연구대상은 전라남도에 소재한 보육교사 321명이며 교사의 심리적 소진, 교사-영유아 상호작용, 정서노동에 관한 설문지 도구를 사용하여 조사하였다. 연구에서 얻어진 결과는 첫째, 교사-영유아 상호작용과 심리적 소진 및 정서노동의 관계를 살펴본 결과 심리적 소진과 교사-영유아 상호작용은 부적인 관계가, 정서노동과 교사-영유아간에는 정적인 관계가 있는 것으로 확인되었다. 둘째, 정서노동은 교사의 심리적 소진에 따른 교사-영유아 상호작용의 부정적인 영향력을 감소시키는 것으로 확인되었다. 이러한 결과는 교사의 심리적 소진이 교사-영유아 상호작용에 미치는 영향을 완화하기 위해 정서노동을 중요하고도 잠재적인 요소로 고려할 필요가 있음을 시사한다. This study was conducted in order to examine how emotional labor affects the relationship between teachers’ burnout and teacher-young children interaction. The subjects of this study were 321 teachers working at child care centers in Jollanam-do. Their burnout, teacher-young children interaction, and emotional labor were surveyed using a questionnaire. In an examination of the correlations among child care teachers’ burnout, teacher-young children interaction and emotional labor, the findings were as follows. Teachers’ burnout was found to have a negative correlation with teacher?young children interaction, and emotional labor was found to have a positive correlation with teacher-young children interaction. Also, emotional labor was found to reduce the negative impact of teachers’ burnout on teacher-young children interaction. These results suggest that emotional labor needs to be considered as an important factor for mitigating the impact of child care teachers’ burnout on teacher-young children interaction.

CD5 Expression Dynamically Changes During the Differentiation of Human CD8+ T Cells Predicting Clinical Response to Immunotherapy

Young Ju Kim,Kyung Na Rho,Saei Jeong,Gil-Woo Lee,Hee-Ok Kim,Hyun-Ju Cho,Woo Kyun Bae,In-Jae Oh,Sung-Woo Lee,Jae-Ho Cho 대한면역학회 2023 Immune Network Vol.23 No.4

Defining the molecular dynamics associated with T cell differentiation enhances our understanding of T cell biology and opens up new possibilities for clinical implications. In this study, we investigated the dynamics of CD5 expression in CD8+ T cell differentiation and explored its potential clinical uses. Using PBMCs from 29 healthy donors, we observed a stepwise decrease in CD5 expression as CD8+ T cells progressed through the differentiation stages. Interestingly, we found that CD5 expression was initially upregulated in response to T cell receptor stimulation, but diminished as the cells underwent proliferation, potentially explaining the differentiation-associated CD5 downregulation. Based on the proliferationdependent downregulation of CD5, we hypothesized that relative CD5 expression could serve as a marker to distinguish the heterogeneous CD8+ T cell population based on their proliferation history. In support of this, we demonstrated that effector memory CD8+ T cells with higher CD5 expression exhibited phenotypic and functional characteristics resembling less differentiated cells compared to those with lower CD5 expression. Furthermore, in the retrospective analysis of PBMCs from 30 non-small cell lung cancer patients, we found that patients with higher CD5 expression in effector memory T cells displayed CD8+ T cells with a phenotype closer to the less differentiated cells, leading to favorable clinical outcomes in response to immune checkpoint inhibitor (ICI) therapy. These findings highlight the dynamics of CD5 expression as an indicator of CD8+ T cell differentiation status, and have implications for the development of predictive biomarker for ICI therapy.

Risk Factor for Cerebral Complications in Patients with Pulmonary Arteriovenous Malformation

( Young-ok Na ),( Hong-joon Shin ),( Hwa Kyung Park ),( Jae-kyeong Lee ),( Hyung-joo Oh ),( Bo-gun Kho ),( Tae-ok Kim ),( Yong-soo Kwon ),( Yu-il Kim ),( Sung-chul Lim ),( Min-seok Kim ),( Cheol-kyu P 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-

Background and objective Pulmonary arteriovenous malformation (PAVM) is rare pulmonary disease. Although most patients with PAVM are asymptomatic, cerebral complications associated with PAVM are fatal. The aim of this study was to evaluate the risk factor for cerebral complications in patients with PAVM. Methods We retrospectively reviewed the medical charts of patients with PAVMs between 2007 and 2021 at two tertiary referral hospitals and one secondary hospital. The diagnosis of PAVMs were made by enhanced chest computed tomography or pulmonary angiography. Results Fifty-five patients diagnosed with PAVMs were enrolled in this study. Most patients were female (89.1%) and median age was 53 years. Thirty patients (54.5%) were detected with PAVMs incidentally without symptoms. Twenty-four patients (43.7%) with PAVMs were treated with angioembolization or surgery. Thirteen patients (23.6%) had cerebral complications. Multivariate analysis with logistic regression showed old age (≥ 65 years) was associated with cerebral complications (odds ratio [OR], 16.2; 95% conference interval [CI], 2.4-108.3; P = 0.004). There was no significant difference in development of cerebral complications according to treatment, however, old age (≥ 65 years) was associated with development of cerebral complications in untreated patients (OR, 18.0; 95% CI, 1.4-218.9; P = 0.023). Conclusions Old age (≥ 65 years) was a risk for development of cerebral complications in patients with PAVM, therefore treatment should be considered in old patients with PAVM.

Behavior of Heavy Metals during Gasification and Melting of Industrial Solid Waste

( Na-rang Kim ),( Young-sik Yoon ),( Dong-ju Kim ),( Jae-hoi Gu ),( Sang-ok Choi ),( In-su Lee ),( Soo-tae Choo ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 3RINCs초록집 Vol.2015 No.-

Waste fed to the gasification and melting process, is discharged into synthesis gas and slag. Major components of the gasification synthesis gas mainly contains H₂, CO, CO₂ and CH₄ and it needs to be purified before use. The industrial solid waste was used for gasification and melting in a pilot plant. The major heavy metals in waste are Zn, Cr, Cu, Pb, Ni, Hg, and Cd, etc. Some of the heavy metals in the waste were discharged volatile metallic vapor or particles with synthesis gas, some were discharged molten slag. Purification process consists of quench scrubber, dedust scrubber, neutral scrubber, desulfur scrubber and wet electrostatic precipitator. Therefore wastewater was discharged from this process. This study investigates the behavior of heavy metals during gasification and melting of industrial solid waste. Hg, Pb, Cd and Zn were analyzed in more than 98% of the scrubber wastewater and Hg, Pb, Cd and Zn concentration were 0.0075, 58.8, 3.8 and 854.3 mg/L, respectively. Cr, Cu was analyzed in more than 63%, 43% of the slag, and Cr, Cu concentration were 9,567.7, 8,076.5 mg/L, respectively.

Translocation of Annexin I to the Nucleus by Epidermal Growth Factor in A549 Cells

Na, Doe Sun,Lee, Soo Ok,Kim, Seung Wook,Rhee, Hae Jin,Park, Young Min The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.1

Annexin 1 (also called lipocortin 1), a 37-kDa member of the annexin family of proteins, has been implicated in the mitogenic signal transduction by epidermal growth factor (EGF). Annexin 1 is phosphorylated by the EGF signal, however, the role of annexin 1 in the EGF signal transduction is still unknown. To transduce extracellular signals into the intracellular targets, selective translocation of the signaling molecules to their targets would be necessary. In this study, we examined the subcellular locations of annexin 1 during EGF signal transduction. Treatment of A549 cells with EGF resulted in the translocation of cytoplasmic annexin 1 to the nucleus and perinuclear region as determined by Western blot and immunofluorescent staining. The nuclear translocation of annexin 1 was inhibited by tyrphostin AG 1478 and genistein, the inhibitors of EGF receptor kinase and downstream tyrosine kinases, respectively. Pretreatment of cells with cyclohexamide did not inhibit the nuclear translocation. The results suggest that nuclear translocation of annexin 1 is controlled by a series of kinase dependentevents in the EGF receptor signaling pathway and may be important in tranducing the signals by EGF.

Cervical Ganglion Block Attenuates the Progression of Pulmonary Hypertension via Nitric Oxide and Arginase Pathways

Na, Sungwon,Kim, Ok Soo,Ryoo, Sungwoo,Kweon, Tae Dong,Choi, Yong Seon,Shim, Hyo Sup,Oh, Young Jun American Heart Association, Inc. 2014 Hypertension Vol.63 No.2

<P>It has been recognized that the sympathetic nervous system is activated in pulmonary arterial hypertension (PAH), and abnormal sympathetic hyperactivity leads to worsening of PAH via endothelial dysfunction. The purpose of this study was to examine whether sympathetic ganglion block (SGB) can treat PAH by increasing the availability of nitric oxide (NO). PAH was induced in rats by 50 mg/kg of subcutaneous monocrotaline. After 2 weeks, daily injections of ropivacaine into the left superior cervical ganglion were repeated for 14 days (monocrotaline-SGB group). Monocrotaline group received sham SGB with saline, whereas control group received saline instead of monocrotaline. PAH was evident in monocrotaline group, with right ventricular systolic pressures (47±4 mm Hg) that were higher than those of controls (17±2 mm Hg), whereas SGB significantly attenuated monocrotaline-induced PAH (35±4 mm Hg). The right/left ventricular mass ratios exhibited similar changes to those seen with right ventricular pressures. Heart rate variability showed significantly higher sympathetic activity in the monocrotaline group. Microscopy revealed a higher proportion of muscular arteries with thicker medial walls in the monocrotaline group, which was attenuated by SGB. Monocrotaline induced arginase hyperactivity, which was in turn decreased by SGB-induced endothelial NO synthase activation. SGB restored monocrotaline-induced hypoactivity of superoxide dismutase. In conclusion, SGB could suppress PAH and the remodeling of pulmonary arteries via inactivation of arginase and reciprocal elevation of NO bioavailability, thus attenuating disproportionate hyperactivation of the sympathetic nervous system.</P>

Understanding the enhanced electrochemical performance of TEMPO derivatives in non-aqueous lithium ion redox flow batteries

Ok, Byeori,Na, Wonjun,Kwon, Tae-Hoon,Kwon, Young-Wan,Cho, Sangho,Hong, Soon Man,Lee, Albert S.,Lee, Jin Hong,Koo, Chong Min Elsevier 2019 Journal of industrial and engineering chemistry Vol.80 No.-

<P><B>Abstract</B></P> <P>Non-aqueous lithium-ion redox flow batteries (Li-RFBs) have recently garnered much interest because of their high operating voltage and energy density. Albeit these outstanding advantages, challenges, such as poor cyclability and efficiency, still remain in employing the practical application. In an attempt to address these problems, a series of 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) and TEMPO derivatives catholytes were prepared and investigated as redox-active materials. Electrochemical evaluation exhibited that the introduction of polar and electron-withdrawing substituents to TEMPO was able to enhance the rate capability and cycling stability, when compared to those with the neat TEMPO. Extensive analysis of the electrochemical properties revealed that the electrophilic heteroatom stabilized the radical as well as alleviated the catholyte degradation. Overall, a careful selection of redox-active species demonstrates great promise in improving the current redox flow battery technology.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>