Rootletin is a Marker for Asthma Exacerbation in Patients with Asthma: Implication for Diesel Exhaust Particles

( Seon-muk Choi ),( An-soo Jang ),( Min-hyeok An ),( Yun-gi Lee ),( Pureun-haneul Lee ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-

Background Diesel exhaust particles (DEP) can cause and exacerbate asthma and COPD. Rootletin is the main component of the ciliary rootlet and functions as a centriole linker connecting the two mother centrioles. The impact of air pollutants on Rootletin in asthma remain to be clarified. Objective: The aim of study was to identify protein expression in normal human bronchial epithelial (NHBE) cells and mice exposed to DEP, and to know the impact of Rootletin on asthma. Methods Rootletin level was checked in the plasma of asthmatic patients and control subjects. The relation of Rootletin with clinical variables in patients with asthma was observed. Rootletin expression was estimated using ELISA, immunoblotting, and immunohistochemical stain. A proteomic approach was used to determine the different expression of proteins at 8hr and 24hr after treatment of DEP 5 ug/ml and 10 ug/ml to NHBE cells. Results Rootletin level had higher concentrations in plasma from patients with asthma (n = 18) than that of healthy controls (n =10). Rootletin level was correlated with FVC, FEV1 %pred. and FEV1/FVC in patients with asthma. NHBE cells following DEP exposure for 8hr and 24hr have altered various protein spots. Rootletin proteibn expression in lung tissue was increased in DEP exposed mice compared to control mice. Rootletin expression was increased in NHBE cells treated house dust mite by Western blotting. Conclusion These Results indicate that Rootletin may be an important role for airway disease caused by DEP, and a marker for asthma exacerbation.

해수 환경에서의 철근과 GFRP리바의 굽힘강도 특성에 관한 연구

한길영,최명선,곽상묵 朝鮮大學校 機械技術硏究所 2003 機械技術硏究 Vol.6 No.1

Fiber reinforced plastics(FRP) attracted muGh attention as a form of concrete reinforcement in the past decade. Compared with existing construction matrials, FRP possesses many advantages such as light-weight. high-strength, corrosion resistant properties, etc. Among other fabrication process of GFRP, pultrusion is one of the promising ones for civil infrastructure application. This paper describes the need for a ductile Fiber Reinforced Plastics (FRP) reinforcement for concrete structures. To promte the degradation of the adhesive condition a t the fiber-matrix micro interface without matrix dissolution loss were carried out in salt water surrounding. The absorption properties and the bending strength were compared about GFRP rebar and steel bar. Due to the fiber permeability absorption of moisture take place through both the fiber and the matrix. The diffusivities obtained for Glass-Polyester are found to be in good agreement with those available in the existing literature.

과민성 장 증후군에서 장 점막 비만세포

최재홍 ( Jae Hong Choi ),나병규 ( Byung Kyu Nah ),임정묵 ( Jung Muk Leem ),배상석 ( Sang Seok Bae ),최기원 ( Ki Won Choi ),채희복 ( Hee Bok Chae ),박선미 ( Seon Mee Park ),윤세진 ( Sei Jin Youn ),성노현 ( Ro Hyun Sung ) 대한소화기기능성질환·운동학회 2004 Journal of Neurogastroenterology and Motility (JNM Vol.10 No.1

목적: 과민성 장 증후군은 임상적으로 매우 흔한 질환이지만 아직까지 그 원인이나 기전이 명확하게 밝혀지지 못한 상황이다. 비만세포는 장관 신경과 평활근의 기능을 변화시키는 중요한 매개체를 가지며 장관 신경과 근육과도 상호작용을 하여 과민성 장 증후군의 병인에 작용할 수 있다고 생각한다. 이에 저자들은 비만세포가 과민성 대장 증후군 환자의 대장 점막에서 정상 및 염증성 질환 환자에 비해서 차이가 있는지 알아보고, 과민성 장 증후군의 아형에 따라서도 차이가 있는지를 알아보고자 하였다. 대상 및 방법: 대상 환자는 충북대학교 병원 내과에서 진료 받은 환자들 중 Rome II 진단 기준에 합당한 환자들을 대상으로 하였으며 과민성 장 증후군 환자는 16명, 정상 대조군 8명, 염증성 대조군은 5명이었다. 모든 환자는 대장내시경 검사를 시행하였고, 맹장, 상행, 하행 결장과 직장 부위에서 생검을 하였다. 비만세포는 트립타제에 대한 항체를 이용한 면역 염색법으로 관찰하였고, H&E 염색을 통하여 중성구, 림프구, 형질세포와 호산구의 수를 측정하여 차이를 비교하였다. 결과: 비만세포수는 하행 결장에서만 과민성 장 증후군(15.2±4.0)에서 정상 대조군(10.9±2.7)과 염증성 대조군(11.0±2.9)보다 유의하게( p=0.02) 높았으며, 다른 부위에서는 유의한 차이가 없었다. 비만세포를 제외하고는 과민성 장 증후군에서 다른 염증세포의 침윤이 증가해 있지는 않았다. 과민성 장 증후군의 증상의 유형에 따라서도 차이가 있어 맹장 부위에서의 비만세포수가 변비형(21.3±3.4)과 통증형(22.7±2.5)이 설사형(14.6±4.2)보다 유의하게 높게 나타났다( p<0.05). 결론: 과민성 장 증후군에서 다른 염증성 및 정상 대조군과 비교하여 하행 결장의 점막에서 비만세포의 수가 유의하게 높았고, 과민성 장 증후군의 증상별 아형에 따른 비교에서는 변비형과 통증형이 설사형 보다 맹장 부분에서 유의하게 높게 관찰되었다. 과민성 장 증후군에서 비만세포가 병인에 관여하며 증상의 아형별에 따라서 그 관여정도가 차이가 존재할수 있음을 시사한다. Backgr ound/Aims: Irritable bowel syndrome (IBS) is ubiquitous but the pathophysiology remains poorly established. There has been increasing interest in the role of mast cells (MC) as they release variable mediators and have receptors for neurotransmitters. The aim of this study was to determine whether MC were increased in the colonic mucosa of IBS patients compared to controls. Methods: Biopsy was done from the cecum, ascending colon, descending colon and rectum of 29 patients: 16 IBS, 8 normal, and 5 inflammatory controls. Tissue was stained immunohistochemically with a monoclonal antibody for human mast cell tryptase for MC, and H&E stain for other inflammatory cells. Results: MC count of IBS patients at the descending colon (15.2±4.0) was significantly higher than that of either normal controls (10.9±2.7) or inflammatory controls (11.0±2.9). In a group of patients with IBS, MC count at the cecum was significantly higher in the constipation-predominant subtype (21.3±3.4) and the pain-predominant subtype (22.7±2.5) than the diarrheapredominant subtype (14.7±4.2). Conclusions: Patients with IBS have higher MC count than controls, and MC count in constipation-predominant IBS patients was significantly higher than diarrhea-predominant IBS patients. These findings suggest that MC might play an important role in IBS. (Kor J Neur ogastr oenter ol Motil 2004;1:57-62)

N-Acetylcysteine Decreases Airway Inflammation and Lung Fibrosis by Modulating Ros and Nrf2 in Mice Model Exposed to Particulate Matter

( Min-hyeok An ),( Seon-muk Choi ),( Yun-gi Lee ),( Pureun-haneul Lee ),( An-soo Jang ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-

Background Air pollutants can induce and incite airway diseases such as asthma. N-acetylcysteine (NAC) affects signaling pathways involved in apoptosis, angiogenesis, cell growth and arrest, redox-regulated gene expression, and the inflammatory response. However, it is not known how NAC change redox-regulated gene expression in asthma mouse model exposed to particulate matter (PM). Methods To investigate the effects of NAC (100 mg/kg) on redox-regulated gene expression and lung fibrosis in a mouse model of asthma exposed to PM. A mice model of asthma induced by ovalbumin (OVA) or OVA plus titanium dioxide (OVA+TiO2) was established using wild-type BALB/c female mice, and the levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and mucin 5AC (Muc5ac) proteins following NAC treatment were examined by Western blotting and immunostaining. In addition, the protein levels of ROS were checked. Results Airway hyperresponsiveness and inflammation, goblet cell hyperplasia, and lung fibrosis were higher in OVA, OVA+TiO2 mice than in control mice. NAC diminished OVA+TiO2-induced airway hyperresponsiveness and inflammation, goblet cell hyperplasia, and lung fibrosis. Levels of ROS, Nrf2 and Muc5 protein were higher in lung tissue from OVA+TiO2 mice than that from control mice, and were decreased by treatment with NAC. Conclusions NAC reduce airway inflammation and responsiveness, goblet cell hyperplasia, and lung fibrosis by modulating ROS and Nrf2.

Track 3 : 컴퓨터활용교육; 아두이노를 활용한 점자 교육 시스템의 설계 및 구현

임재원 ( Jae Won Lim ),장선필 ( Seon Pil Jang ),박상빈 ( Sang Been Park ),최경묵 ( Kyeong Muk Choi ),박종화 ( Jong Hwa Park ) 한국컴퓨터교육학회 2014 한국컴퓨터교육학회 학술발표대회논문집 Vol.18 No.1

시각장애인을 위한 기기들로 상당히 많은 제품이 있다. 하지만 시각장애인을 위한 기기들은 점자를 알아야 사용 가능한 것들이 대부분이기에, 반복학습을 통해 점자를 스스로 학습할 수 있는 기기를 제작하였다. 이 기기는 많은 사람들이 배울 수 있게 경제성과 편리성을 갖추었다. 아두이노를 사용하여 편리성을 갖추었고, 스위치의 위치, 크기, LED 등으로 편리성을 갖추었다. 점자 교육 시스템의 사용성 테스트를 위하여 시각장애인협회를 방문하였고, 점자 교육에 도움이 될 수 있다는 결론을 내렸다.

알코올성 간경변 환자에서 위대망 정맥류 출혈로 인한 자발성 혈복강 1 예

채희복,윤세진,박선미,성노현,배일헌,임정묵,장이찬,최기원,신종성,지정훈 대한간학회 2001 Clinical and Molecular Hepatology(대한간학회지) Vol.7 No.3

Portal hypertension often leads to the development of several collateral vessels that shunt blood flow from the portal to the systemic circulation. The rupture of intra-abdominal varix is an unusual complication of portal hypertension that can lead to life-threatening hemoperitoneum of the patient is herrmdynamically unstable due to massive intra-abdominal bleeding, exploratory laparotomy should be performed on the patient. There are several reported cases of intra-abdominal variceal bleeding such as paraumbilical varix, the varix from the small intestine and proximal colon etc. Spontaneous hemoperitoneum caused by the rupture of gastroepiploic vein varix, however, has not been repzted in Korm, to the best of our knowledge. We wiU discuss a patient with portal hypnsion due to liver cirrhosis who pnmented with acute intra-abdominal bleeding. During the laparotomy, he was found to have a rupture of the gastnxpiploic vein. 'Ihe vessel was ligated, and the patient recovered uneventfully by operative variceal ligation. (Korean J Hepatol 2001;7:336-340)

Anti-Inflammatory Effects of Peanut Sprout and Oil Extracts In a Systemic Anaphylaxis Food Allergy Mouse Model

Won Kyung Yang(양원경),Dong Seon Kim(김동선),Eun Jung Son(손은정),Yun Mi Lee(이윤미),Yang Chun Park(박양춘),Pureunchowon Lee(이푸른초원),Seon Muk Choi(최선묵),Seung Hyung Kim(김승형) 한국약용작물학회 2019 한국약용작물학회 학술대회논문집 Vol.2019 No.1

Quantitative analysis of anthraquinone compounds from Senna tora seeds at different ripening stages

Joon-Soo Sim,Soo-Ji Choi,Ji-Seon Kim,In-Jin Ha,Chang-Muk Lee,Bum-Soo Hahn 한국당과학회 2016 한국당과학회 학술대회 Vol.2016 No.07

Senna tora (Cassia tora), a member of Leguminosae, is widely distributed in south-east Asian countries. The dried or roasted seed of Senna tora has been used as one of Oriental medicines for centuries for vision-improving, anti-asthenia and diuretic. Furthermore, many reports related to various biological and pharmacological activities of Senna tora have been published such as anti-inflammatory, anti-hepatotoxic, radical scavenging, anti-allergic, anti-mutagenic, antipyretic, hypoglycemic, anti-fungal and anti-bacterial effects. In this study, we have focused on characterizing and quantifying of anthraquinone compounds from Senna tora seeds during different ripening stages. Ultra-high performance liquid chromatography coupled with diode array detector and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-DAD-ESI-Q-TOF-MS) was used to investigate the compositional changes of anthraquinone compounds that were involved in ripening of Senna tora seeds. Furthermore, non-targeted UHPLC-ESI-Q-TOF-MS data were subjected to principal component analysis and discriminant analysis to find the potential characteristic components from different ripening stages of Senna tora seeds.

Metabolic Analysis and the Anther Culture of Safflower Germplasms

So Young Park(박소영),Ji Seon Kim(김지선),Mun Ja Choi(최문자),Seon Kyeong Lee(이선경),Sun yim Bae(배순임),Joon Soo Sim(심준수),Chang Muk Lee(이창묵),Bum Soo Hahn(한범수) 한국약용작물학회 2019 한국약용작물학회 학술대회논문집 Vol.2019 No.2

The Impact of Environmental Pollutants on Barrier Dysfunction in Respiratory Disease

Lee Pureun-Haneul,Park Shinhee,Lee Yun-Gi,Choi Seon-Muk,An Min-Hyeok,Jang An-Soo 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.6

Respiratory epithelial cells form a selective barrier between the outside environment and underlying tissues. Epithelial cells are polarized and form specialized cell-cell junctions, known as the apical junctional complex (AJC). Assembly and disassembly of the AJC regulates epithelial morphogenesis and remodeling processes. The AJC consists of tight and adherens junctions, functions as a barrier and boundary, and plays a role in signal transduction. Endothelial junction proteins play important roles in tissue integrity and vascular permeability, leukocyte extravasation, and angiogenesis. Air pollutants such as particulate matter, ozone, and biologic contaminants penetrate deep into the airways, reaching the bronchioles and alveoli before entering the bloodstream to trigger airway inflammation. Pollutants accumulating in the lungs exacerbate the symptoms of respiratory diseases, including asthma and chronic obstructive lung disease. Biological contaminants include bacteria, viruses, animal dander and cat saliva, house dust mites, cockroaches, and pollen. Allergic inflammation develops in tissues such as the lung and skin with large epithelial surface areas exposed to the environment. Barrier dysfunction in the lung allows allergens and environmental pollutants to activate the epithelium and produce cytokines that promote the induction and development of immune responses. In this article, we review the impact of environmental pollutants on the cell barrier in respiratory diseases.