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Islam, K.B.M.S.,Rahman, M.S.,Ershaduzzaman, Md.,Taimur, M.J.F.A.,Jang, Hyung-Kwan,Song, Hee-Jong The Korean Society of Veterinary Service 2010 韓國家畜衛生學會誌 Vol.33 No.1
An enzyme-linked immnnosorbent assay (ELISA) has been performed for the detection of the prevailing toxinotypes of Clostridium perfringens obtained from conventional culturing of intestinal contents of goats which have died of suspected enterotoxaemia. The test was found effective to detect the toxins as well as types of the organism with less time and labor. The most prevailing type of C. perfringens causing enterotoxaemia in goat was C. perfringens type D (68.75%) and followed by C. perfringens type B (25%) and C (6.25%). No C. perfringens type A was detected. This study showed an intelligible picture of prevailing toxinotypes of C. perfringens in goats in Bangladesh. The use of the ELISA for the detection of clostridial types and toxins allows the differential diagnosis of C. perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C. perfringens.
( K. B. M. S. Islam ),( M. S. Rahman ),( Md. Ershaduzzaman ),( M. J. F. A. Taimur ),( Hyung Kwan Jang ),( Hee Jong Song ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.1
An enzyme-linked immnnosorbent assay (ELISA) has been performed for the detection of the prevailing toxinotypes of Clostridium perfringens obtained from conventional culturing of intestinal contents of goats which have died of suspected enterotoxaemia. The test was found effective to detect the toxins as well as types of the organism with less time and labor. The most prevailing type of C. perfringens causing enterotoxaemia in goat was C. perfringens type D (68.75%) and followed by C. perfringens type B (25%) and C (6.25%). No C. perfringens type A was detected. This study showed an intelligible picture of prevailing toxinotypes of C. perfringens in goats in Bangladesh. The use of the ELISA for the detection of clostridial types and toxins allows the differential diagnosis of C. perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C. perfringens.
Major degradable polycations as carriers for DNA and siRNA
Islam, M.A.,Park, T.,Singh, B.,Maharjan, S.,Firdous, J.,Cho, M.H.,Kang, S.K.,Yun, C.H.,Choi, Y.,Cho, C.S. Elsevier Science Publishers 2014 Journal of controlled release Vol.193 No.-
Non-viral gene delivery systems are one of the most potential alternatives to viral vectors because of their less immunogenicity, less toxicity and easy productivity in spite of their low capacity of gene transfection using DNA or silencing using siRNA compared to that of viral vectors. Among non-viral systems, the polycationic derivatives are the most popular gene carriers since they can effectively condense nucleic acids to transfer into the cells, especially the polyethylenimine (PEI) which has been used as a golden standard polymer owing to its high buffering ability for endosomal escape of gene to be expressed. However, PEI has severe problems for its toxicity due to the high positive charge density and non-degradability although the toxicity of PEI depends on its molecular weight (MW) and structure. Therefore, a considerable attention has been paid on synthesis of degradable PEI derivatives using low MW one because low MW PEI is much less toxic than high MW PEI. Other degradable polycationic gene carriers such as polyamidoamines (PAA) and cyclodextrin (CD)-based polycations are also in a significant interest because of their high transfection efficiency with low toxicity. This review in detail explains the recent developments on these three major degradable polycations as promising carriers for deoxyribonucleic acid (DNA) and small interfering RNA (siRNA).
Computation of transonic internal flow around a biconvex airfoil with cavity
M. Mostaqur Rahman,A. B. M. Toufique Hasan,A.K.M. Sadrul Islam,S. Matsuo,T. Setoguchi 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.6
At transonic flow conditions, unsteady self-excited shock waves are frequently observed in several modern internal aeronautical applicationssuch as in turbine cascades, compressor blades, butterfly valves, fans, nozzles, diffusers and so on. The appearance of shock oscillationoften causes serious problems such as aero-acoustic noise, non-synchronous vibration (NSV), intense drag rise, high cycle fatiguefailure (HCF) and buffeting. In recent years, the effect of various passive means on the airfoil are investigated both experimentally andnumerically to find the effectiveness as a shock control device. In the present study, the transonic internal flow around an airfoil using acavity as a passive means of shock control was investigated numerically. Computational results are validated with available experimentaldata. Results showed that the airfoil with cavity significantly reduced the flow field unsteadiness such as the amplitude of pressure oscillationand root mean square of pressure oscillation.
Je Hee Lee,Seon Young Choi,Yoon-Seong Jeon,Hye Ri Lee,김은진,Binh Minh Nguyen,Nguyen Tran Hien,M. Ansaruzzaman,M. Sirajul Islam,Nurul A. Bhuiyan,S.K. Niyogi,B.L. Sarkar,G. Balakrish Nair,Dae Shick Kim,An 한국미생물학회 2009 The journal of microbiology Vol.47 No.6
Analysis of the CTX prophage and RS1 element in hybrid and altered Vibrio cholera O1 strains showed two classifiable groups. Group I strains contain a tandem repeat of classical CTX prophage on the small chromosome. Strains in this group either contain no element(s) or an additional CTX prophage or RS1 element(s) on the large chromosome. Group II strains harbor RS1 and CTX prophage, which has an El Tor type rstR and classical ctxB on the large chromosome.