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Human granulocytic anaplasmosis as a cause of febrile illness in Korea since at least 2006
Yi, Jongyoun,Kim, Kye-Hyung,Ko, Mee Kyung,Lee, Eun Yup,Choi, Su Jin,Oh, Myoung-don AMERICAN SOCIETY OF TROPICAL MEDICINE AND HYGIENE 2017 The American journal of tropical medicine and hygi Vol.96 No.4
<P>Human granulocytic anaplasmosis (HGA) is a tick-borne rickettsial zoonosis with fever, thrombocytopenia, and leukopenia. HGA has been reported in Korea in 2013 but it is uncertain how long it has existed. A retrospective study was conducted on patients who underwent bone marrow examination due to fever and cytopenia, with no clear hematologic or microbiologic causes, from 2003 through 2012. Laboratory diagnosis was made by detecting 16S rRNA genes of Anaplasma phagocytophilum from the stored blood samples. Among the 70 patients, five (7.1%) HGA cases were found, and the earliest case dated back to 2006. Two cases met the diagnostic criteria of hemophagocytic lymphohistiocytosis (HLH) and were fatal. Although HGA has been prevalent in Korea since at least 2006, it is not always diagnosed and has posed a possible lethal health risk to the people in Korea. HGA should be considered as a cause of fever with cytopenia or HLH.</P>
In vitro antiviral activity of ribavirin against severe fever with thrombocytopenia syndrome virus
( Myung Jin Lee ),( Kye-hyung Kim ),( Jongyoun Yi ),( Su Jin Choi ),( Pyoeng Gyun Choe ),( Wan Beom Park ),( Nam Joong Kim ),( Myoung-don Oh ) 대한내과학회 2017 The Korean Journal of Internal Medicine Vol.32 No.4
Background/Aims: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by severe fever with thrombocytopenia syndrome virus (SFTSV), a novel bunyavirus. As yet, there is no effective antiviral therapy for SFTS. Ribavirin is a broad-spectrum antiviral agent, which has been tried for treatment of SFTS. In this study, antiviral activity of ribavirin against SFTSV has been investigated. Methods: Vero cell-grown SFTSV strain Gangwon/Korea/2012 was treated with ribavirin at various concentrations. Antiviral activity of ribavirin was evaluated by inhibition of the SFTSV cytopathic effect in Vero cells and quantification of viral RNA load in culture supernatant using one-step real-time reverse transcription polymerase chain reaction. Cytotoxicity of ribavirin was determined by a tetrazolium- based colorimetric method. Results: Ribavirin reduced SFTSV titers in a dose-dependent manner, with a half-maximal inhibitory concentration ranged from 3.69 to 8.72 μg/mL. Cytopathic effects were reduced as ribavirin concentration increased. No significant cytotoxicity was detected at ribavirin concentrations of ≤ 31.3 μg/mL. Conclusions: Ribavirin exhibited inhibitory activity against SFTSV replication in vitro, which suggests that ribavirin can be used as a potential antiviral agent for SFTS.
Kim, Namhee,Lee, Seung Hee,Yi, Jongyoun,Chang, Chulhun L. The Korean Society for Laboratory Medicine 2015 Annals of Laboratory Medicine Vol.35 No.5
<P><B>Background</B></P><P>Peptide nucleic acid (PNA) probes are artificial DNA analogues with a hydrophobic nature that can penetrate the mycobacterial cell wall. We evaluated a FISH method for simultaneous detection and identification of <I>Mycobacterium tuberculosis</I> (MTB) and non-tuberculous mycobacteria (NTM) in clinical respiratory specimens using differentially labeled PNA probes.</P><P><B>Methods</B></P><P>PNA probes targeting the mycobacterial 16S ribosomal RNA were synthesized. The cross-reactivity of MTB- and NTM-specific probes was examined with reference strains and 10 other frequently isolated bacterial species. A total of 140 sputum specimens were analyzed, comprising 100 MTB-positive specimens, 21 NTM-positive specimens, and 19 MTB/NTM-negative specimens; all of them were previously confirmed by PCR and culture. The PNA FISH test results were graded by using the United States Centers for Disease Control and Prevention-recommended scale and compared with the results from the fluorochrome acid-fast bacterial stain.</P><P><B>Results</B></P><P>The MTB- and NTM-specific PNA probes showed no cross-reactivity with other tested bacterial species. The test results demonstrated 82.9% agreement with the culture results with diagnostic sensitivity of 80.2% and diagnostic specificity of 100.0% (kappa=0.52, 95% confidence interval: 0.370-0.676).</P><P><B>Conclusions</B></P><P>Dual-color PNA FISH showed high specificity for detecting and identifying mycobacteria in clinical specimens. However, because of its relatively low sensitivity, this method could be more applicable to culture confirmation. In application to direct specimens, the possibility of false-negative results needs to be considered.</P>
국립공원 종사자의 중증열성혈소판감소증후군 혈청유병률 및 위험요인
김동휘(Dong-Hwi Kim),김계형(Kye-Hyung Kim),이종윤(Jongyoun Yi),고미경(Mee Kyung Ko),박성준(Sung-Jun Park),유석주(Seok-Ju Yoo),이관(Kwan Lee),박지혁(Ji-Hyuk Park) 한국농촌의학 지역보건학회 2021 농촌의학·지역보건 Vol.46 No.3
이번 연구는 임업 종사자 중 하나인 국립공원 종사자를 대상으로 진드기 매개 감염병인 SFTS 혈청유병률을 확인하고 관련된 위험요인을 조사하고자 시행하였다. 2016-2017년에 실시한 “쯔쯔가무시증 및 라임병 고위험군에 대한 감염실태 조사” 사업으로 수집된 763명의 혈청 검체와 설문조사 결과를 이용하였고, 혈청분석은 Double-antigen sandwich enzyme-linked immunosorbent assay로 IgG와 IgM을 포함한 전체 항체를 검사하였다. 국립공원 종사자 763명의 SFTS 혈청유병률은 1.4%였다. 일반적 특성 중 성별로는 여성이 1.7%로 더 높았고, 연령별로는 30-49세에서 2.0%로 가장 높았다. 학력에 따라 고등학교 졸업 이하가 2.5%로 더 높았고, 기관별로는 국립공원사무소 분소 및 기타기관이 1.7%로 더 높았다. 근무기간에 따라 15년 이상이 2.2%로 가장 높았으며, 지역적으로는 북부에서 3.1%로 가장 높았다. 다변량 로지스틱 회귀분석 결과 학력, 탐방 프로그램 운영, 매일 작업복을 갈아입음이 최종 모델에 포함되었다. 이 중 탐방 프로그램을 운영할 경우는 교차비가 3.48(95% 신뢰구간=1.01-12.01)로 통계적으로 유의하게 높았다. 학력과 매일 작업복을 갈아입음은 통계적으로 유의하지 않았다. 이번 연구는 진드기에 노출될 수 있는 고위험 직업군인 국립공원 종사자를 대상으로 SFTS의 혈청유병률을 파악하기 위한 조사를 국내에서 최초로 시행하였다는 점에서 의미가 크다. 국립공원 종사자는 진드기 매개 감염병의 고위험 직업군임에도 불구하고 작업 중 SFTS 예방을 위한 작업환경 및 습관과 관련된 예방활동이 부족한 것으로 생각되며, 국립공원 종사자에 대한 체계적인 예방 교육 및 훈련을 보다 강화할 필요가 있다. Objectives: This study was carried out to understand the seroprevalence and risk factors for severe fever with thrombocytopenia syndrome (SFTS) among the Korea National Park Service (KNPS) workers. Methods: We used the stored serum samples (763) and survey results collected from the previous investigation on scrub typhus and Lyme disease among the KNPS workers during 2016-2017. The serum samples were analyzed by double-antigen sandwich enzyme-linked immunosorbent assay, which was used to test the total antibody including IgG and IgM. Results: The SFTS seroprevalence among the KNPS worrkers was 1.4%. In multivariate logistic analysis, the national park exploration programs (odds ratio, 3.48; 95% confidence interval, 1.01-12.01) was significantly associated with the seroprevalence of SFTS. Conclusion: This study was the first serological study of SFTS among forestry workers in South Korea. Although the KNPS workers are at a high-risk group of SFTS, the prevention activities related to the working environment and habit was insufficient. Thus, systematic prevention education and training for the KNPS workers need to be strengthened.