항생제 다제내성균 Staphylococcus aureus SA2로부터 분리한 테트라사이클린 내성 플라스미드 pKH6의 염기서열

이대운,윤성준,김우구,신철교,임성환,이백락,문경호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

한국에서 임산균주로부터 분리한 테트라사이클린(Tc) 내성 플라스미드 pKH6의 전체 염기서열을 결정하여 가장 대표적인 Tc 내성 플라스미드인 pT181과 비교하였다. pKH6의 전체 길이는 4439bp로 pT181과 동일하게 나타났으며 염기서열은 7개의 염기에서 차이를 보였다. 전부 다 염기쌍이 치환된 형태로 나타났으며 그 중 3개는 coding 부위에서 일어났다. coding 부위에서 일어난 경우에도 1개는 동일한 아미노산을 coding 하였기 때문에 pT181과 차이가 없었으며 RepC와 Pre 단백질에 있어 각각 1개의 아미노산에서 차이를 나타내었다. The complete nucleotide sequence of pKH6, a tetracycline-resistance (Tc^r) plasmid isolated from multi-drug resistant Staphlococcus aureus SA2, has been determined and compared with that of the staphylococcal Tc^r plasmid pT181. The nucleotide sequences of the two plasmids are in agreement except for 7 nucleotides. All differences are caused by base pair substitutions. Among 6 substitutions, 3 occurred in coding regions. However, only tow base substitutions in coding regions resulted in changes of amino acid sequences in two different ORFs of repC and Pre proteins.

기능성 Catechol-O-Methyltransferase 유전자 다형성과 한국인 정신분열병의 관련성

박태원,홍영준,박임순,김형섭,최인미,배수영,윤경식,강대희 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : Catechol-O-Methyltransferase(COMT)는 카테콜아민 신경전달물질의 불활성화를 담당하는 효소로서, 정신분열병과 관련된 후보 유전자로서 알려져 왔다. 최근, 기능성 COMT유전자 다형성과 정신분열병의 관련성이 제기되었는데, 한국인 정신분열병에서도 이러한 관련성이 있는지를 살피기 위해 본 연구를 시행하였다. 방 법 : 정신분열병으로 진단된 환자군 103명과 성별 및 연령별로 1:1 짝짓기 대조군 사이에 유전자형과 대립유전자의 분포를 비교하였다. 또한, 환자군은 공격적 행동, 가족력, 아동기 발병 여부에 따라 두 군으로 나누어 비교하였다. DNA의 다형성 부의를 중합효소연쇄반응에 의해 증폭한 후 ,NlaⅢ제한효소법과 전기영동을 실시하여 유전자형을 판별하였다. 결 과 : 환자군 전체와 대조군 사이에 유전자형과 대립유전자 빈도의 차이가 없었다. 가족력이 있는 환자군을 대조군과 비교하였을 때는, 대립유전자 L을 포함하는 유전자형과 그렇지 않은 유전자형의 분포에 있어 유의한 차이를 보였다(p=0.02, OR=3.9, 95% Cl=1.1∼14.3). 결 론 : 한국인 정신분열병과 기능성 COMT 유전자 다형성 사이에 유의한 관련성이 없었다. 따라서, 기능성 COMT 유전자 다형성이 한국인 정신분열병의 발병에 영향을 주지 못한다. 그러나, 정신분열병 가족력과 기능성 COMT유전자 다형성의 관련성이 시시돠어, 향후 본 연구에서 나타난 방법론적인 문제점을 보완하고 추가 대상자를 확보한 연구가 필요하다. Objectives : Catechol-O-methyltransferase(COMT) is involved in the degradation of catecholamine meurotransmitters and has been investigated as a candidate gene in schizophrenia. Recently, possible relationship between functional COMT gene polymorphism and schizophrenia has been suggested. To address the possible role of functional COMT gene polymorphism in the predisposition to schizophrenia, we carried out an association study in Korean schizophrenic patients and controls. Methods : One hundred and three Korean inpatients diagnosed as schizophrenia and 103 age and sex matched controls were selected as study subjects. Patients were subgrouped into two groups on the basis of history of aggressive behavior, family history of schizophrenia and related disorders, and age at onset. We determined COMT genotypes using PCR of the relevant region followed by digestion with NlaⅢ and electrophoresis. Results : No significant differences of allele and genotype frequencies were noted between patients and controls. However, when patients were categorized by the presence of family history of schizophrenia and related disorders, patients with family history showed almost 4 fold higher frequency of having COMT L allele containing genotype compared to controls(p=0.02, OR=3.9, 9.5% CI=1.10-14.33). Conclusion : Although our results do not support an association between functional polymorphism of COMT gene and schizophrenia overall, the findings suggest an association between functional COMT gene polymorphism and familial schizophrenia. Further studies with large samples are needed to confirm this association.

CT 유도하의 경피적 폐생검 : 임상적 의의 및 합병증에 관한 연구 A Study of Clinical Significance and Complication

박재성,백상현,차장규,홍현숙,김대호,박성진,조준희,임한혁,이혜경 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.2

Purpose : To determine the diagnostic accuracy and predicting factor of pneumothorax on CT-guided percutaneous transthoracic needle biopsy. Materials and Methods : Using automated biopsy devices, 107 CT-guided percutaneous needle biopsies of pulmonary lesions were performed. Final diagnosis was made with operation or other methods. We retrospectively analyzed the frequency of complications and diagnostic yields of 107 biopsies. The clinical features, treatment, and courses of complications were reviewed, and risk factors of pneumothorax such as depth and size of lesion, CT emphysema score of whole lung and pathway of needle, and the result of pulmonary function test were analyzed. Result : 96 biopsies(89.7%) yielded sufficient tissue for pathologic evaluation. For cases of malignant and benign disease, sensitivity was 91.5% and 87.5% respectively; the corresponding figures for diagnostic accuracy were 88.1% and 77.1%. So, sensitivity and diagnostic accuracy of percutaneous transthoracic needle biopsy was 89.7% and 83.2%. Eighteen(16.8%) of 107 patients developed pneumothorax and 3 of these required application of chest tube or percutaneous catheter drainage. In the group with pneumonthorax, the lesion was significantly smaller(p=0.029) and higher CT emphysema score of pathway of needle(p=0.032). But there were no significant difference between two groups(with or without pneumothorax) for depth of lesion, pulmonary function test(% predictive value of FEV1 and FEV1/FVC) and CT emphysema score of whole lung. Conclusion : CT-guided transthoracic needle biopsy of the pulmonary lesions is safe and easy method of obtaining specimens of good quality and quantity. It may be an useful procedure in the diagnosis of localized pulmonary lesions. For the prediction of pneumothorax, size of lesion and CT emphysema score of pathway of a needle are significant risk factors.

담배나방 세포질다각체병 바이러스의 동정 및 병원성에 관한 연구

Dae Joon Im,Dong Sook Jang,Kui Moon Choi,Seok Kwon Kang 한국응용곤충학회 1991 한국응용곤충학회지 Vol.30 No.3

담배나방 유충에서 분리한 세포질다각체병 바이러스의 형태, 다각체 단백질 및 핵산의 전기영동상과 바이러스의 병원성을 조사하여 본 바이러스를 이용한 담배나방의 생물적 방제 이용성을 검토하고자 본 실험을 수행하였다. 다각체의 형태는 외관상 6각형으로 0.5~3.7 크기이고 바이러스 입자는 정 20면체로 55nm였다. SDS-PAGE에 의한 다각체 단백질은 단일 롤리?타이드인 24.3 Kd와 5개의 작은 구성분으로 이루어졌다. 바이러스입자는 7개의 폴리?타이드로 구성되어 있으며 분자량은 28.0~133.6 Kd였다. 바이러스 게놈은 10개의 조각으로 된 총 분자량 18.08 Md인 이본쇄 RNA로 각 조각의 분자량 범위는 0.65~2.79 Md이였다. 3령 유충에 대한 담배나방 세포질 다각체병바이러스의 은 이었으며 의 농도에서 에서 16.4일이었다. A cytoplasmic polyhedrosis virus isolated from the oriental tobacco budworm, Heliothis assulta (HaCPV), was studied on morphology of the polyhedron and virus particles, analysis of viral protein and nucleic acid, and bioassay of the HaCPV to determine the feasibility of application as a microbial control agent. The shape of polyhedron was hexagonal ranging 0.5-3.7 and the virus particles were icosahedral outline measured 55 nm in diameter. Polyhedral protein was composed of a major polypetide of 24.3 Kd and 5 minor components and virus particle had seven polypeptides ranging in 28.0 Kd-133. 6 Kd by the SDS-P AGE. The genome of virus was segmented with 10 double stranded RNA in the total mol. wt. of 18.08 Md ranging in 0.65 Md -2.79 Md. The values of the HaCPV to the 3rd instar of H. assulta larvae were calculated to . The values in the concentration of was 16.4 days.

A Survey on the Kinds of Leaf Rollers in Mulberry Trees

Im, Dae-Joon,Paik, Hyun-Jun Korean Society of Sericultural Science 1982 한국잠사곤충학회지 Vol.23 No.2

뽕나무를 가해하는 잎말이나방류의 종류와 우점종을 조사하기 위해 1979년부터 잠업시험장 포장에서 조사한 결과, 1. 뽕나무 가해잎말이바방류는 뽕나무 해충으로 미기록종인 뽕나무애기잎말이나방(가칭, Olethreutes hemiplaca MEYRICK)과 Olethreutes doubledayana BARRET을 포함 7종이 조사되었다. 2. 우리나라에서 우점종은 Olethreutes hemiplaca MEYRICK였으며 애모무늬잎말이나방(Adoxophyes orana F.&R.)이 그 다음이였다. This survey was carried out at the Sericultural Experiment Station. Suweon to clarify the kinds of leaf rollers damaging mulberry leaves and determine the dominant species of them. The results obtained are as follow: 1. A total of seven species including 2 unrecorded species, Olethreutes hemiplaca MAYRICK. and Olethreutes doubledayana BARRET, were found as mulberry attacking leaf rollers. 2. The dominant species of mulberry leaf rollers in Korea was identified as O. hemiplaca M. followed by Adoxophyes orana B.

뽕나무 가해(加害) 잎말이나방 류(類)에 관(關)한 조사(調査)

임대준 ( Dae Joon Im ),백현준 ( Hyun Jun Paik ) 한국잠사학회 1981 한국잠사곤충학회지 Vol.23 No.2

뽕나무를 가해하는 잎말이나방류의 종류와 우점종을 조사하기 위해 1979년부터 잠업시험장 포장에서 조사한 결과, 1. 뽕나무 가해잎말이나방류는 뽕나무 해충으로 미기록종인 뽕나무애기잎말이나방(가칭, Olethreutes hemiplaca MEYRICK)과 Olethreutes doubledayana BARRET을 포함 7종이 조사되었다. 2. 우리나라에서 우점종은 Olethreutes hemiplaca MEYRICK였으며 애모무늬잎말이나방(Adoxophyes orana F.&R.)이 그 다음이였다. This survey was carried out at the Sericultural Experiment Station. Suweon to clarify the kinds of leaf rollers damaging mulberry leaves and determine the dominant species of them. The results obtained are as follow: 1. A total of seven species including 2 unrecorded species, Olethreutes hemiplaca MAYRICK, and Olethreutes doubledayana BARRET, were found as mulberry attacking leaf rollers. 2. The dominant species of mulberry leaf rollers in Korea was identified as O. hemiplaca M. followed by Adoxophyes orana B.

A Review on the Application of Nanotechnology in Food Processing and Packaging

Seong-In Cho,Yong-Rok Kim,Joon Woo Lee,Dae-Sup So,Yong-Jin Cho,Hyun Kwon Suh,Tu San Park,Seoung-Im Oh,Ji-Eun Im 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.4

Currently, nanotechnology is widely applied in various industrial fields and is rapidly emerging as a promising future technology. In food industries, nanotechnology is used to enhance food quality and safety. Numerous cutting-edge studies on the advantages of nanotechnology have been conducted in the fields of food processing, food ingredients and additives, food packaging, and food engineering for optimal health. The market for these areas of research has grown steadily, and is expected to continue to do so. Because of this, R&D for nanotechnology that can be used effectively in food industries is being performed by several companies, as well as in academic research institutions around the world. This review describes the recent global R&D trends that have been in progress for two key areas: food processing and food packaging.

CASE REPORT : Psoriasis Induced by Trastuzumab

Dae Hun Kim,Nam Ji Jeong,Myung Im,Young Lee,Young Joon Seo,Jeung Hoon Lee 대한피부과학회 2013 Annals of Dermatology Vol.25 No.2

LETTER TO THE EDITOR : Encephalocraniocutaneous Lipomatosis without Neurologic Anomalies

Dae Hun Kim,Seung Bae Park,Young Lee,Myung Im,Young Joon Seo,Si Hwan Choi,Jeung Hoon Lee 대한피부과학회 2012 Annals of Dermatology Vol.24 No.4

Oxygen stoichiometry controlled sharp insulator-metal transition in highly oriented VO₂/TiO₂ thin films

Im, Ji-Seok,Anoop, Gopinathan,Sohn, Min Kyun,Kang, Dae Joon,Jeong, Sang Yun,Lee, Sanghan,Jo, Ji Young ELSEVIER 2018 CURRENT APPLIED PHYSICS Vol.18 No.6

<P><B>Abstract</B></P> <P>The insulator-metal transition (IMT) in vanadium dioxide (VO<SUB>2</SUB>) which occurs above room temperature (67 °C) is highly sensitive to atomic defects caused by oxygen stoichiometry. The strained growth and the degree of oxygen deficiency in VO<SUB>2</SUB> epitaxial films result in lowering of transition temperature below room temperature as well as the broadening of transition parameters such as transition width and hysteresis width, which limit its application potential. Here we demonstrate the growth of highly oriented strain-relaxed VO<SUB>2</SUB> thin films on (001)-oriented TiO<SUB>2</SUB> substrates at various oxygen partial pressures, exhibiting the narrow transition and hysteresis width. The cross-sectional transmission electron microscopy and x-ray diffraction analyses of the films reveal the highly oriented growth of insulating monoclinic VO<SUB>2</SUB>. The IMT parameters associated with temperature-dependent phase transition vary with the oxygen partial pressure used during the deposition. The presence of multiple and mixed valence states of vanadium in the films was confirmed by Raman and XPS analyses. We have achieved a narrow transition width (2.3 °C) and hysteresis width (1.2 °C) through controlling the oxygen stoichiometry during the growth of VO<SUB>2</SUB>/TiO<SUB>2</SUB> films.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Highly oriented epitaxial VO<SUB>2</SUB> films were grown on (001)-oriented TiO<SUB>2</SUB> substrate. </LI> <LI> Insulator to metal transition (IMT) parameters of the VO<SUB>2</SUB> film varies with the oxygen stoichiometry. </LI> <LI> IMT in VO<SUB>2</SUB> varied depending on the degree of crystallinity and microstructure of the films. </LI> <LI> The VO<SUB>2</SUB> film grown at high deposition oxygen pressure showed a sharp transition with a narrow hysteresis width. </LI> </UL> </P>