Orotic acid 유발 지방간 rat와 mouse의 중성지질 대사

車載英,조영수 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4

Orotic acid 1% 투여에 의해 유발된 脂防肝 SD-계 rat와 kud : ddY mouse의 중성지질대사에 미치는 n-6계 지방산의 효과에 대하여 검토하였다. 그 결과 rat에 orotic acid무첨가 식이와 비교할때 1% orortic acid및 n-6계 PUFA(linoleic acid)가 함유된 반합성 식이 투여군에서 간장의 triglyceride함량은 유의적으로 증가하였고, 반면 혈청 triglyceride 함량은 감소하였다.이에 비해, mouse는 orotic acid 1% 함유식이 투여에 의해 간장triglyceride함량은 rat와 달리 증가하는 경향이었다. 중성지질 최종제한 단계 효소인 phosphatic acid phosphohydrolase의 활성은 triglyceride합성을 촉진시켰으며, orotic acid첨가 식이를 투여한 rat의 간장 cytosol및 microsome에서도 같은 경향이었다. 이러한 것으로부터 이 효소의 활성은 rat에 있어서 지방간 항진에 관여한다는 것이 강하게 시사되었다. Effects of 1% dietary orotic acid on triglyceride metabolism were examined in SD-rats and Kud : ddY mice. When rats were fed semisynthetic diet containing 1% orotic acid and n-6 polyunsaturated fatty acid(linoleic acid), the hepatic triglyceride content was increased significantly by about 3-fold and the serum triglyceride content was decreased compared with orotic acid-free diet. In contrast to rats which respond to orotic and consumption with increases in hepatic triglyceride content, mice did not so respond. The rats-limiting step in triglyceride synthesis is catalyzed by the enzyme phosphatic acid phosphohydrolase (EC 3.1.3.4) which is present in the liver cytosol and microsomes of rate fed orotic acid diet. This finding suggests that the activity of this enzyme may play a role in the fatty liver formation in rats.

혈관내(血管內) Helium-Neon Laser 조사(照射)가 혈중(血中) Total Cholesterol 및 Triglyceride 수치변화(數値變化)에 미치는 영향(影響)

백은탄,민재영,국윤형,오상덕,라수연,임승만,Paik, Eun-Tan,Min, Jae-Young,Koog, Yun-Hyung,Oh, Sang-Deog,Ra, Su-Yoen,Lim, Seung-Man 대한한방내과학회 2000 大韓韓方內科學會誌 Vol.21 No.5

Objective : This study was designed to assess effects of He-Ne laser irradiation on high-level of Total Cholesterol and Triglyceride. Methods : The 76 patients who have been checked Total Cholesterol and Triglyceride high were divided into a study group of patient who were irradiated and into a control group of patients who did not receive irradiation.. Then, patients rechecked Total Cholesterol and Triglyceride after 1 month later. Results : In patients of study group, The level of Total Cholesterol and Triglyceride was decreased. But In comparison with control group, there was no significant effect of decrement of Total Cholesterol and Triglyceride. Conclusions : These results suggest that He-Ne laser irradiation may have no significant decrement effects on high-level of Total Cholesterol and Triglyceride.

Development of paclitaxel-loaded liposomal nanocarrier stabilized by triglyceride incorporation

Hong, Soon-Seok,Choi, Ju Yeon,Kim, Jong Oh,Lee, Mi-Kyung,Kim, So Hee,Lim, Soo-Jeong DOVE MEDICAL PRESS 2016 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.11 No.-

<P>Studies have highlighted the challenge of developing injectable liposomes as a paclitaxel (PTX) carrier, a challenge attributable to the limitations in liposomal stability caused by PTX loading. Poor stability of PTX-loaded liposomes is caused by PTX-triggered aggregation or fusion of liposomal membranes and is exacerbated in the presence of PEGylated lipid. In the present study, the effect of triglyceride incorporation on the stability of PTX-loaded/PEGylated liposomes was explored. Incorporation of a medium chain triglyceride Captex 300 into saturated phosphatidylcholine (PC)-based liposomes (1,2-dimyristoyl-<I>sn</I>-glycero-3-phosphocholine [DMPC]:cholesterol [CHOL]:N-(Carbonyl-methoxypolyethyleneglycol 2000)-1, 2-distearoyl-<I>sn</I>-glycero-3-phospho-ethanolamine [PE-PEG]), produced a fine, homogeneous, and membrane-filterable PTX-loaded liposomes fulfilling the requirement of an injectable lipid formulation. Triglyceride incorporation also greatly inhibited the time-dependent leakage of PTX from saturated PC-based liposomes, which appears to be mediated by the inhibition of liposome fusion. In contrast, triglyceride incorporation induced the destabilization and PTX leakage of unsaturated PC-based liposomes, indicating the opposite effect of triglyceride depending on the fluidity status of PC constituting the liposomal membrane. PTX release profile and the in vitro and in vivo anticancer efficacy of triglyceride-incorporated DMPC:CHOL:PE-PEG liposomes were similar to Taxol<SUP>®</SUP> while the toxicity of liposomal PTX was significantly lower than that of Taxol. Taken together, triglyceride incorporation provided an injectable PTX formulation by functioning as a formulation stabilizer of PEGylated/saturated PC-based liposomes.</P>

지방간과 혈중 중성 지방 측정치와의 관련성

유준,권혁중,조동영 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

There were many studies about correlation between fatty liver and serum triglyceride level. However, we have experienced many fatty liver patients that actually did not show high serum triglyceride level. So, we conducted this study to reconfirm the con-elation between fatty liver and serum triglyceride level. We randomly selected 118 patients in Health Examination Center. We divided the selected materials into the patient and control group by the level of fasting serum triglyceride level, 200mg/d.L. We analyzed frequency and distribution of factors(age, body mass index, alcohol, diabetes mellitus) related to fatty liver between patient and control group. Then we analyzed odd ratio of fatty liver among two groups. There were 22 peoples in patient group and 96 people in control group. There were no statistical differences in the mean body mass index, the frequency of overweight, the frequency of alcohol users, the mean fasting serum glucose, and the frequency of diabetes mellitus patients between patient and control group. The odd ratio of fatty liver among two groups did not mean statistical difference. However, there was statistical significant difference in the frequency of body mass index on the base of multiple logistic regression analysis of serum triglyceride level, body mass index, and frequency of diabetes mellitus, and alcohol users between patient and control group. There was no correlation between fatty liver and serum triglyceride. But there was correlation between fatty liver and body mass index.

Differential Benefit of Statin in Secondary Prevention of Acute Myocardial Infarction according to the Level of Triglyceride and High Density Lipoprotein Cholesterol

김경환,김철환,정명호,안영근,김영조,조명찬,김완,김종진,Other Korea Acute Myocardial Infarction Registry Investigators 대한심장학회 2016 Korean Circulation Journal Vol.46 No.3

Background and Objectives: The differential benefit of statin according to the state of dyslipidemia has been sparsely investigated. Wesought to address the efficacy of statin in secondary prevention of myocardial infarction (MI) according to the level of triglyceride andhigh density lipoprotein cholesterol (HDL-C) on admission. Subjects and Methods: Acute MI patients (24653) were enrolled and the total patients were divided according to level of triglyceride andHDL-C on admission: group A (HDL-C≥40 mg/dL and triglyceride<150 mg/dL; n=11819), group B (HDL-C≥40 mg/dL and triglyceride≥150mg/dL; n=3329), group C (HDL-C<40 mg/dL and triglyceride<150 mg/dL; n=6062), and group D (HDL-C<40 mg/dL & triglyceride≥150mg/dL; n=3443). We evaluated the differential efficacy of statin according to the presence or absence of component of dyslipidemia. Theprimary end points were major adverse cardiac events (MACE) for 2 years. Results: Statin therapy significantly reduced the risk of MACE in group A (hazard ratio =0.676; 95% confidence interval: 0.582-0.785;p<0.001). However, the efficacy of statin was not prominent in groups B, C, or D. In a propensity-matched population, the result wassimilar. In particular, the benefit of statin in group A was different compared with group D (interaction p=0.042)Conclusion: The benefit of statin in patients with MI was different according to the presence or absence of dyslipidemia. In particular,because of the insufficient benefit of statin in patients with MI and dyslipidemia, a different lipid-lowering strategy is necessary in thesepatients.

청소년의 혈청 지질에 관한 연구

은백린,독고영창 고려대학교 의과대학 1993 고려대 의대 잡지 Vol.30 No.3

Compelling evidence exists that the atherosclerotic process begins in childhood and progresses slowly into adulthood, at which time it leads frequently to coronary heart disease ; that high blood cholesterol levels early in life play a role in the development of adult atherosclerosis; that eating patterns and genetics affect blood cholesterol levels and coronary heart disease risk ; and that lowering levels in children and adolescents will be beneficial. A cross sectional study on serum lipids in Korean adolescents was carried out in three areas in 1992. Serum lipids, i. e. total cholesterol, HDL, cholesterol and triglyceride concentrations were determined and LDL cholesterol and so called atherogenic index were calculated in 465 adolescents aged 12 to 17 years. The results are as follows ; 1. The mean serum total cholesterol level is 141.83±28.53mg/dl(male 133.95±25.90mg/dl, female 150.20±28.87mg/dl), HDL cholesterol 49.84±10.93mg/dl(male 48.18±9.95mg/dl, female 51.61±11.65mg/dl), LDL cholesterol 74.32±26.38mg/dl(male 68.59±24.70mg/dl, female 80.40±26.80mg/dl), triglyceride 92.33±47.03mg/dl(male 93.09±61.09mg/dl, female 91.52±24.58mg/dl) and atherogenic index 1.98±0.89(male 1.89±0.78, female 2.06±0.98). 2. In male adolescents, the 75th percentile for total cholesterol is 150.0mg/dl, for LDL cholesterol 83.0mg/dl, and for triglyceride 100.0mg/dl. The 95th percentile for total cholesterol, LDL cholesterol, and triglyceride are 179.4mg/dl, 111.2mg/dl and 170.0mg/dl, respectively. The 5th and 25th percentile for HDL Cholesterol are 33.0mg/dl and 41.0mg/dl, respectively. 3. In female adolescents, the 75th percentile for total cholesterol is 167.0mg/dl, for LDL cholesterol 97.0mg/dl and for triglyceride 105.0mg/dl. The 95th percentile for total cholesterol, LDL cholesterol, and triglyceride are 200.8mg/dl, 131.4mg/dl and 142.0mg/dl, respeetively. The 5th and 25th percentile for HDL Cholesterol are 36.0mg/dl and 44.0mg/dl, respectively. 4. The serum levels of total cholesterol, HDL cholesterol and LDL cholesterol are higher in female than male adolescents except triglycerides. 5. There are significant correlations between serum total cholesterol level and height, between HDL cholesterol level and weight, arm circumference, Kaup index, obesity index, triceps skinfold, abdominal skinfold and subscapular skinfold, between triglyceride concentration and diastolic blood pressure. Atherogenic index also has correlations with arm circumference, Kaup index, obesity index, triceps skinfold, abdominal skinfold and subscapular skinfold.

혈중지질에 대한 진피추출물의 유효성 평가를 위한 인체적용시험: 무작위배정, 이중맹검

이진상 ( Jin Sang Lee ),도은주 ( Eun Ju Do ),곽민아 ( Min A Kwak ),박현진 ( Hyun Jin Park ),하일도 ( Il Do Ha ),성기준 ( Ki Jun Sung ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.1

Objectives:This study was performed to evaluate the efficacy of extract from Citri Pericarpium (CP) on lipid-lowering effect in semi-healthy subjects with increased serum levels of total cholesterol and triglyceride. Methods: A experimental group (n=45) and placebo group (n=46) were randomly established based on the plasma lipid profiles (triglyceride, total cholesterol etc.) in the semi-healthy subjects, then all subjects randomly took experimental or placebo capsules, which contain 1,200 mg of CP or the same dose of soluble starch per day with regular meals for 8 weeks by double-blind method. Results: One-hundred forty (140) subjects were screened, then 91 subjects enrolled in the study. No serious adverse events were reported for trial period. The CP treatment showed statistically significant decrease in serum levels of triglyceride both at 4-weeks (p<0.01) and at 8-weeks (p=0.0001). Futhermore, there was significant difference in the triglyceride levels between CP-treated and placebo group. The CP-treated but not placebo group, experienced decrease in serum levels of total cholesterol at 8-weeks. Then there was no significant difference in the total cholesterol levels between the two groups. In addition, statistically significant difference of AST, ALT, γ-GT serum levels wasn``t shown before and after trial in two groups. Conclusion: These results demonstrate that CP can significantly suppress the elevated triglyceride but not total cholesterol level. Therefore, CP may play a role in the improvement of blood lipid levels, as an effective functional food.

과체중 혹은 비만인 한국인 성인남녀에서 모발 칼슘-마그네슘 비율과 Triglyceride-Glucose 지수 및 중성지방/고밀도 콜레스테롤 비율의 연관성: 예비연구

심효영,정선영,이용제,박병진 대한가정의학회 2020 Korean Journal of Family Practice Vol.10 No.6

Background: The interaction between calcium and magnesium as a risk modifier for insulin resistance may be largely overlooked because the strictregulatory system in blood has been thought to maintain such homeostatic interactions under tight control. This study aimed to investigate therelationship between the calcium-magnesium ratio in hair with the triglyceride glucose index (TyG index) and triglyceride/HDL-cholesterol ratio (TG/HDL ratio) among overweight or obese Korean individuals. Methods: This cross-sectional study included 56 participants fulfilling the inclusion criterion of a body mass index of 23 kg/m2or more. Intra-abdominalvisceral adipose tissue (VAT) was measured by fat measurement computed tomography, while calcium and magnesium levels were measured byhair mineral analysis. A high TyG index and a high TG/HDL ratio were defined as >9.3 and >4.0, respectively (>75th percentile). Using multiplelogistic regression analysis, we examined the associations between the TyG index, TG/HDL ratio, and calcium–magnesium ratio in hair. Results: The mean age, body mass index, and VAT were 45.3 years, 28.5 kg/cm2, and 137.4 cm2, respectively. Compared to the controls, the odds ratios(95% confidence intervals) for a high TyG index and a high TG/HDL ratio were 16.03 (1.32–194.23) and 9.98 (1.05–94.98) per one increment of log(calcium-magnesium ratio), respectively, after adjusting for age, sex, body mass index, visceral adipose tissue, white blood cell count, totalcholesterol, hypertension medication, diabetes medication, and dyslipidemia medication. Conclusion: We found that the calcium-magnesium ratio in hair was positively and independently associated with the TyG index and TG/HDL ratio. 연구배경: 칼슘 및 마그네슘은 상호 작용하여 인슐린 저항성에 영향을 줄 수 있으나 혈중 농도는 항상성 유지 체계로 이들 간의 상호작용을 규명하기에 불충분한 경우가 많다. 본 연구에서는 모발 내칼슘-마그네슘 비율과 TyG 지수 및 중성지방/고밀도콜레스테롤 비율과의 관련성을 알아보고자 하였다. 방법: 일차진료 비만클리닉을 방문한 체질량지수 23 kg/m2이상의수진자 중 모발 미네랄검사와 복부 체지방 컴퓨터 단층촬영을 시행한 57명을 대상으로 하였다. 다중로지스틱 회귀분석을 통해 칼슘,마그네슘 및 이들 간의 비율이 ‘High TyG index’ (TyG 지수≥9.3; 75퍼센타일)과 ‘High TG/HDL ratio’ (TG/HDL 비율≥4.0; 75퍼센타일)과 연관성이 있는지를 평가하였다. 결과: 연구 대상자 평균 연령은 45.3±10.7세였으며, 체지량 지수는28.5 kg/m2, 내장지방은 137.4 cm2이었다. 칼슘-마그네슘 비율(로그변환)은 혼란변수를 보정한 후 TyG 지수 및 중성지방/고밀도 콜레스테롤 비율과 각각 교차비와 95% 신뢰구간 16.03 (1.32–194.23), 9.98(1.05–94.98)로 유의한 양의 연관성을 보였다(P=0.029, P=0.045). 결론: 과체중 이상의 한국인 성인 남녀에서 모발 내 칼슘-마그네슘의 비율이 TyG 지수 및 중성지방/고밀도 콜레스테롤 비율과 유의한 양의 연관성을 보였다. 추후 대규모 전향적 연구를 통한 이들 간의관계 규명이 필요할 것으로 보인다.

Steptozotocin 유발 당뇨 흰쥐에서 Tabebuia avellandae의 항당뇨 효과

정춘식,정기화 德成女子大學校 藥學硏究所 1997 藥學論文誌 Vol.8 No.1

Hypoglycemic effect of Tabebuia avellandae was investigated in the streptozotocin(STZ)-induced diabetic rats. Diabetes was induced in male Sprague-Dawley rats by injections of STZ(45 mg/kg, i.v.). Rats weighing 200-250g were divided into 6 groups:normal, STZ-control, hexane fr., CHCI_3 fr., BuOH fr. and H_2O fr. gourp. Normal and STZ-control rats received 3% tween 80 only. Four groups of diabetic rats were administered orally at doses of 100, 400, 300 and 400 mg/kg/day of hexane, CHCI_3, BuOH and H_2O fr. respectively. Fractions were administered orally to the rats for 7 days after STZ injection. All rats were anesthetized with ether, blood samples were taken by cardiac puncture for clinical chemistry and the rats were killed by exsanguination. Liver, kidney, heart and spleen were removed, weighed and analyzed. We measured glucose, protein, cholesterol and triglyceride levels in the plasma and glycogen, cholesterol and triglyceride levels in liver. The extent of blood glucose decrement in rats administered H_2O fraction was greater than that in the STZ-control rats. The serum cholesterol and triglyceride levels were significantly lowered by administration of H_2O fraction compared with those of STZ-control group. Treatment of rats with Tabebuia avellandae fractions caused decreases in STZ-induced elevation of cholesterol and triglyceride. Liver triglyceride level was significantly lowered hexane and BuOH fraction group compared with STZ-control group. These results suggest that H_2O fraction of Tabebuia avellandae has the hypoglycemic action against STZ-induced diabetic rats.

平胃導痰湯이 高脂肪食餌性 高脂血症 白鼠에 미치는 影響

朴元煥 대한동의병리학회 1996 동의생리병리학회지 Vol.10 No.2

高脂血症은 血液속의 脂質性分이 보통 이상으로 增加된 狀態이며, 高脂血症의 發生原因은 原發性과 績發性이 있으며 이로 말미암아 發生한 高脂血症은 過多한 脂肪質의 蓄積으로 動脈硬化症, 肥滿症, 糖尿病등을 招來할 수 있다. 특히 脂肪質의 以上蓄積은 動脈硬化症의 原因 중에서도 가장 위험한 仁者로 알려져 있다. 오늘날 成人病을 誘發시키는 發病因子중에 高脂血症이 차지하는 比率이 날로 增加하는 趨勢이므로, 韓醫學의 痰陰·瘀血理論과 接近을 시키고 韓醫學的 方法으로 脂肪質의 蓄積으로 오는 成人病을 解決하는 것이 硏究의 目標이며 이를 위해 高脂血症의 病態模型에 痰濁, 濕濁등의 治療에 많이 活用되며 平胃導痰湯을 復用시킨 후, 高脂肪性高脂血症病態誘發과 이에 대한 治療效果르 硏究하기 위하여, 白鼠의 血淸脂質에 包含되어 있는 酵素活性度에 대한 影響을 觀察하였다. 血淸중 total-cholesterol, triglyceride와 β-lipoprotein 含量에 미치는 影響은 正常群에 비해서 對照群에서 모두 增加되어 나타났으며, 平胃導痰湯을 復用시킨 實驗群에서는 對照群에 비해서 모두 減少되는 變化가 나타났다. 血淸 중 HDL과 phospholipid 含量에 미치는 影響은 正常群에 비해서 對照群에서 모두 減少되어 나타났으며, 平胃導痰湯을 復用시킨 實驗群에서 對照群에 비해서 增加되는 變化가 나타났다. 또한 血淸중 total-cholesterol 含量에 대한 HDL 含量의 比率과 total-cholesterol 含量에 대한 phospholipid 含量의 比率은 正常群에 비해서 對照群에서 모두 減少되었으며, 對照群에 비해서 實驗群에서는 모두 增加되는 比率이 나타났다. 平胃導痰湯은 高脂肪性固形飼料를 自由攝取시켜 만들어지는 白鼠의 高脂血症病態에 有意할 만한 治療效果가 있는 것으로 認定되었으며, 아울러 高脂肪性??食物로 인한 脂質大事障碍나 그로 인한 高脂血症 및 動脈硬化疾患의 治療硏究에 더 많은 應用이 可能하다고 思料된다. Diet is the fundamental condition for human beings to live in good health. An improper diet can affect the physiological functions of the viscera and reduce the organism's resistance to disease. Thus becoming pathogenic factors. In particular, a heavy and fatty diet can increase opportunity for adults to become infected by diseases. Blood lipids are composed of cholesterol, triglyceride, phospholipid and free fatty acids. Hyperlipidemia implies the increased levels of cholesterol and triglyceride in the plasma. It is closely related to atherosclerosis. As blood lipids are transported in the form of lipoprotein, hyperlidemia can also manifest itself as hyperlipoproteinemia. Hyperlipidemia is included in the categoreis of “xuan yun”(vertigo), “tan zhuo”(phlegm syndrome) and others in Oriental medicine. Retention of phlegm and fluid is pathological products in the body, which manifest themselves through the course of illness. When retention of phlegm and fluid developes, it can act directly or indirectly, effect certain tissues or organs in the human body. Thus, causing new phtholoical chagnes, and forming various syndromes. therefore it become also a group of pathogenic factors. In oriental medicine differentiation and treatment of hyperlipidemia are divided into deficiency of the Liver-yin, Kidney-yin and retention of Damp-heat in the interior. Therapeutic principle of deficiency of the Liver-yin and Kidney-yin, is nourishing the liver-yin and kidney-yin. Therapeutic principle of retention of Damp-heat in the lnterior is eliminating pathogenic damness and heat. Pungwidodamtang had used a prescirption for this experiment, and was recorded in “Zung in Mak Chi〈症因脈治〉”. Pungwidodamtang is a comman remedy for the treatment of dampness-phlegm, stomachache, vomiting, nausea, salivation and also applied widly for other uses, This prescription is combined Pungwisan with Dodamtang. Pung-wi-san is a representative prescription for drying dampness evil and promoting the function of the spleen. The action of Pung-wi-san is regulating the function of the spleen and strengthening the stomach and activating circulation of vital energy. Pung-wi-wan has a therapy action which is used for cases due to stagnation of dampness evil in the spleen and stomach and functional disorders of vital energy. Thses can be manifested as abdominal fullness, anorexia, vomiting, nausea, a heavy sensation over the body, tiredness, loose stools, white and thick greasy fur on the tongue, show pulse, etc. The action of Dodamtang is drying dampness, eliminating phlegm and activating vital energy. Do-dam-tang has a therapy action used primarily for cases due to phlegm syndrome accompanied with coma or coldness of extremities. These can be manifested as dizziness, feeling of stagnation over the chest, nausea, vomiting, restlessness, anorexia, while and smooth fur on the tongue, wiry and smooth pulse. Hence, Pungwidodamtang is the prescription to use for invigorating the spleen, and promote diuresis to eliminate the wetness-evil from the lower warmer. Thus it can be used as a clinical application for the treatment hyperlipidemia. In order to study the effects of the Pungwidodamtang on high lipid diet-hyperlipidemia rats, white rats were divided into 4 groups. One group(Control group) was induced by high lipid solid feed for 35 days. Two groups(test 1, 2 groups) were treated Pungwidodamtang for 28 days from experiment-1 week after. The other group(Normal group)was not induced. Then, each of these groups were compaired and measured in the quantity of total-cholesterol, HDL, triglyceride, phospholipid, β-lipoprotein, the rate of total-cholesterol versus HDL and total-cholesterol versus phospho-lipid in their serum. 1. We noticed the decrease in the quantity of “total-cholesterol, triglyceride and β-lipoprotein” in sample-groups 1 and 2 as compaired with the control groups. Particularly noticeably significant results approached during the 3th, 4th, 5th, week in sample-group 1. 2. We noticed the increase in the quantity of HDL and phospholipid in sample-groups 1 and 2. However the quantity of HDL showed an increase in each week of the experiment whereas the quantity of phosopholipid only showed significant increase in the 4th and 5th weeks. 3. Rate of total-cholesterol versus HDL and total-cholesterol versus phospholipid were increased in both sample-groups 1 and 2 as compaired with the control groups. Thus, the change of the quantity of total-cholesterol, triglyceride, phospholipid and β-lipoprotein was influenced with hyperlipid solid and Pung-wi-do-dam-tang. Pung-wi-do-dam-tang had a noticeable therapeutic effect for hyper lipid diet-hyperlipidemia pattern. Therefore, Pung-wi-do-dam-tang can be applied as an effective therapy for disturbance of lipid metabolism induced hyper lipid diet, Hyperlipidemia and atherosclerosis induced disturbance of lipid metabolism.