볶음처리에 따른 콩나물과 땅콩 새싹 추출물의 이화학적 특성 및 관능적 특성 변화

홍성준(Seong Jun Hong),조진주(Jin-Ju Cho),부창국(Chang Guk Boo),윤문연(Moon Yeon Youn),이승민(Seung-Min Lee),신의철(Eui-Cheol Shin) 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.4

본 연구는 콩나물과 땅콩 새싹에 대한 볶음 과정에서 생성되는 이화학적 특성과 관능적 특성의 변화를 확인하였다. 두 새싹 샘플 모두에서 볶음 과정 후 총 페놀 함량과 총 플라보노이드 함량이 증가하는 경향을 나타내었고, 이와 같은 영향으로 DPPH 라디칼 소거능이 증가함을 확인하였다. 또한 볶음 과정을 통해 새싹 내 수분함량이 감소하였으며, 이로 인해 카페인 함량, 염도 및 당도가 증가하였다. pH의 경우 볶음 과정 2분 후 콩나물에서 다소 증가하였지만 나머지 구간에서는 별다른 차이가 일어나지 않았고, 볶음 과정을 거친 땅콩 새싹은 점차 pH가 감소하는 경향을 나타내었다. 색도의 경우 두 새싹 모두에서 lightness는 볶음 후 점차 감소하였고, browning intensity는 볶음 후 점차 증가하는 경향을 나타내었다. GC/MS와 후각 검사를 이용하여 분석한 결과 두 새싹 모두에서 benzene acetaldehyde를 콩나물 향과 땅콩 새싹 향으로 확인하였다. 전자혀를 통한 맛 패턴분석에서 볶음처리 후 콩나물은 umami의 증가와 쓴맛 감소의 결과를 나타내었으며, 땅콩 새싹의 경우 신맛, 짠맛 및 감칠맛의 증가가 나타났고 쓴맛은 감소하는 결과를 나타내었다. 전자코 시스템을 이용하여 콩나물과 새싹나물에 존재하는 다양한 향기 성분을 확인하였다. 본 연구 결과는 향후 새싹나물의 산업적 이용을 위한 기초연구 자료로서의 활용이 가능할 것으로 판단된다. This study investigated the physicochemical and sensory properties of roasted bean and peanut sprouts. The total phenol content and total flavonoid content in both extracts were increased after the roasting process, thereby confirming an increase in the antioxidant capacity. Moisture content of both samples was reduced after roasting, which consequently increased the caffeine content, salinity, and sugar levels. A slight increase of pH level was observed in bean sprouts after 2 min of roasting, but there was no significant difference in the remaining sections; however, roasting of peanut sprouts resulted in a gradual decrease in pH. Assessment of chromaticity after roasting revealed gradually decreasing lightness, and gradually increasing browning intensity. GC/MS analysis and olfactory test confirmed benzene acetaldehyde as the volatile compound present in both bean and peanut sprouts. Subsequent to roasting, analysis of taste pattern through electronic tongue revealed increased umami and decreased bitter taste in bean sprouts, and increased sour, salty and sweet taste in peanut sprouts. Analysis of the electronic nose determined a total of 31 volatile compounds in bean sprouts, and 36 volatile compounds in peanut sprouts. This study can be used as basic research data on the physicochemical and sensory properties of bean and peanut sprouts that have been subjected to the roasting process.

고품질 땅콩나물 생산을 위한 최적 재배수온 조건 확립

이규빈 ( Gyu Bin Lee ),박은지 ( Eun Ji Park ),허유 ( You Heo ),손병구 ( Beung Gu Son ),최영환 ( Young Whan Choi ),이용재 ( Yong Jae Lee ),박영훈 ( Young Hoon Park ),서정민 ( Jeong Min Suh ),강점순 ( Jum Soon Kang ) 한국환경과학회 2015 한국환경과학회지 Vol.24 No.6

The present study was conducted to development mass production methods for peanut sprouts that is considered as a field of blue ocean among the agricultural products. ``Jopyeong`` was the best as a major cultivar for peanut sprouts production. The manual for the production of high-quality peanut sprouts is as following. Germination temperature appropriate for production of high-quality peanut sprouts was 27℃. Peanut sprouts at the growth stage of 8th day, and older plants with advanced growth showed deteriorated merchantable and eating quality. Resveratrol compound was not found in the seeds, but its highest amount was detected from 9-day old sprouts. The best water temperature applicable to high quality peanut sprout production was 25℃. The growth of peanut sprout was inhibited by the high temperatures above 35℃ and low temperatures below 15℃.

땅콩 품종을 이용한 싹나물 특성 평가

배석복,하태정,이명희,황정동,심강보,박장환,박금룡,백인열 한국작물학회 2011 한국작물학회지 Vol.56 No.4

땅콩의 새로운 수요개발을 위해 국내 육성품종 37종에 대한 땅콩 싹나물 비교시험을 통하여 싹나물에 적합한 우량품종을 선발하고 생육형질 상호간의 관계를 조사여 싹나물 연구를 위한 기초 자료자료로 활용하고자 수행한 결과는 다음과 같다. 본 시험에 이용한 각 품종의 100립중은 조광 56 g에서 호광 142 g까지 넓게 분포하였다. 전체품종의 하배축길이는 팔광 4.3 cm에서 대신 5.8 cm 분포하고 평균 5.1 cm였으며 하배축의 직경은 평균 6.9 mm 미광 5.0 mm에서 대청 8.0mm까지 걸쳐 분포하였다. 뿌리길이는 대청 11.1 cm에서 미광 20.6 mm에 분포하였으며 평균 14.6 cm이였다. 상배축의 길이는 평균 1.8 cm로서 대풍 0.8 cm에서 미광 4.6 cm에 걸쳐 분포하였다. 미광은 하배축이 가늘고 짧으며, 상배축과 뿌리가 가장 길었고 기능성 성분 resveratrol 함량(88.2ug/g )도 높았다. 중소립종 조광은 하배축의 길이(5.0 cm)가 짧은 편이나 하배축 직경(7.8 mm)은 통통하고 싹나물의 크기가 균일하며 수율은 820%로 가장 높았으며 특히 깨끗한 외관 품질을 가져 땅콩 싹나물로 적합한 특성을 가졌다. 땅콩 싹나물 형질 상호간의 상관관계에서 땅콩 종자의 100립중은 싹나물의 resveratrol함량, 성묘율, 수율과는 부(-)의 상관관계를 나타냈고, 묘중, 곡묘 하배축 개열성과는 정(+)의 상관관계를 나타내었다. 또한 싹나물 뿌리 길이는 상배축의 길이, resveratrol 함량, 수율과는 정의 관계를 나타내고 하배축 직경과 개열성과는 부의 관계를 나타냈다. This experiment was conducted to select suitable cultivars and evaluate growth characteristics to get basic information for sprouting peanut. On sprouting peanut, it showed a rapid increase in trans-resveratrol content that has effects on anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular in mouse. For this experiment, characteristics of peanut sprouts were tested in 37 cultivars grown for 7 days at 26~circC temperature. There were a lots of variations in the growth characteristics among cultivars as followers : The range of 100 grain weight was 56 to 142 g, hypocotyl length was 4.3 cm to 5.8 cm, diameter of hypocotyl was 5.0 to 8.0 mm, epicotyl length was 0.8 cm to 4.6 cm, seedling ratio per seed number was 84% to 100%, weight per seedling was 4.9 g to 8.4 g, the rate of hypocotyl cleavage was 0% to 46%, the content of trans-resveratrol was 22.5~;~mug/g to 88.2~;~mug/g and sprout yield was 360% to 820%. The selection points considered were high sprout yield, high seedling rate, high resveratrol content, low brownish cotyledon, no hypocotyl cleavage, and fat hypocotyl etc. The best cultivar selected was 'Jokwang' that showed 7.8 mm diameter, clean cotyledon color, 100% seedling rate, 0% hypocotyl cleavage, 63.3~;~mug/g resveratrol, and 820% sprouting yield. This cultivar was expected to be of use as a new food and nutraceutical material. Relationship between growth characteristics showed that root length had significant positive correlations with epicotyl length, resveratrol content and sprouting yield but negative correlations with hypocotyl diameter and cleavage. Hundred grain weight showed negative correlations with resveratrol content, seedling rate and sprouting yield but positively correlated with curved hypocotyl rate and hypocotyl cleavage positively. This result showed small grain seed will be more appropriate for sprouting peanut.

The Preventive Effects of Nanopowdered Peanut Sprout-added Caciocavallo Cheese on Collagen-induced Arthritic Mice

Kim, Dong-Hwi,Chang, Yoon Hyuk,Kwak, Hae-Soo Korean Society for Food Science of Animal Resource 2014 한국축산식품학회지 Vol.34 No.1

The present study was carried out to investigate the effects of nanopowdered peanut sprout-added Caciocavallo cheese (NPCC) on the prevention and treatment of rheumatoid arthritis in DBA/IJ mice immunized with type II collagen. After the induction of arthritis, the mice were being divided into five groups: (1) normal, no immunization; (2) CIA, collagen-induced arthritis; (3) MTX, collagen-induced arthritis treated with methotrexate (0.3 mg/kg body weight); (4) CC, collagen-induced arthritis treated with Caciocavallo cheese (0.6 g/d); (5) NPCC, collagen-induced arthritis treated with nanopowdered peanut sprout-added Caciocavallo cheese (0.6 g/d). Nanopowdered peanut sprout was ranged from 300 to 350 nm, while regular powdered peanut sprouts were ranged from 50 to $150{\mu}m$. The NPCC group had considerable reductions of clinical scores and paw thicknesses at the end of experiment as compared to the CIA group. In the serum analysis, the TNF-${\alpha}$, IL-$1{\beta}$, IL-6 and $IgG_1$ levels in the NPCC group have decreased by 69.4, 75.9, 66.6, and 61.9%, respectively, when compared to the CIA group. The histological score and spleen index of the NPCC group were significantly lower than the CIA group. In conclusion, the feeding NPCC method could delay and/or prevent the rheumatoid arthritis in the collagen-induced arthritis mouse model. Based on this study, nanopowdered peanut sprouts could be applied to various functional cheeses.

땅콩나물용 품종선발과 고함량 레스베라트롤 생육단계 구명

박은지(Eun-Ji Park),이규빈(Gyu-Bin Lee),허유(You Heo),손병구(Beung-Gu Son),최영환(Young-Whan Choi),이용재(Yong-Jae Lee),박영훈(Young- Hoon Park),임채신(Chae-Shin Lim),강점순(Jum-Soon Kang) (사)한국생물환경조절학회 2015 생물환경조절학회지 Vol.24 No.2

땅콩나물의 재배에 적합한 온도와 품종, 그리고 최적 레스베라트롤 생산을 위한 생육단계를 구명하고자 본 연구를 수행하였다. 땅콩나물용 적정품종을 선발하고자 8가지 국내외 육성품종의 외형적 특징, 발아율, 발아세, 유묘활력을 조사하였다. 그 결과 소비자의 요구를 반영하여 세근 발생량이 낮고 하배축이 굵은 ‘조평’이 가장 적절하였고, 땅콩나물 재배에 적합한 온도는 27℃로 판단되었다. 레스베라트롤 함량이 높은 땅콩나물의 적정 생육시기를 구명하고자 치상 후 생육조사와 동시에 HPLC를 이용한 레스베라트롤 함량을 조사한 결과 레스베라트롤은 치상 후 9일차에 17.0μg/g 이상의 높은 함유량을 나타내었다. 따라서 땅콩나물은 생산적 측면과 레스베라트롤의 함량을 고려했을 때 치상 후 9일차에 땅콩나물을 수확하는 것이 가장 적합할 것으로 판단된다. This study was conducted to find out optimum cultivars, and growth temperature and stage in peanut sprout for resveratrol production. Morphological characteristic, days to 50% of the final germination percentage(T50) and early growth vigor were measured in 8 different peanut varieties. In result ‘Jopyeong’ appeared to be the most appropriate cultivar for peanut production because of its lower contamination rate, lesser lateral root formation, and thicker hypocotyl length. Optimum temperature for growing peanut sprouts was determined 27. Content of resveratrol was examined by high performance liquid chromatography(HPLC) to investigate appropriate growth stage of peanut sprouts for resveratrol production. Resveratrol was higher than 17.0μg/g in peanuts sprouts 9 days after plating. Considering peanut sprout’s shapes and content of resveratrol into account, it was most appropriate to harvest in 9-day after germination.

Effect of plasma-activated water on peanut seed germination and vegetative growth in a hydroponic system

송일찬,전형원,PRIATAMARYZAADITYA,Gayathri Subramani,고기성,Lee Young Koung 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.4

Peanut sprouts have recently been reported to be rich in some nutrients, including vitamin C, resveratrol, antioxidants, and folate, and are consumed as functional food. However, the effect of indirect plasma treatment, such as plasma-activated water (PAW), on the germination rate and growth of peanut remains largely unknown. Here, we evaluated the effect of PAW produced using a surface dielectric barrier discharge (SDBD) device on germination and vegetative growth in peanut. SDBD-generated PAW had significantly higher nitrate content than tap water (TW). Compared with the TW group, PAW treatment increased the germination rate of peanut seeds by more than twofolds at 3 days after planting; however, there was no significant difference in final germination rate between the PAW and TW groups at 7 days after seeding. In addition, PAW treatment significantly improved biomass-related morphological traits in peanut sprout compared with TW treatment. At the cellular level, PAW treatment increased cell size in the hypocotyl in both the horizontal (115%) and longitudinal (209%) directions, resulting in increased hypocotyl growth. Moreover, histological analysis showed that PAW treatment significantly enlarged parenchyma cell size in hypocotyl pith tissue but did not affect cell number. Overall, these results showed that PAW treatment improved seed germination and growth by enlarging parenchyma and epidermal cell size in hypocotyl, resulting in increased biomass in peanut sprouts. QRT–PCR results showed that auxin response and growth-related genes (AhARF5, AhIAA13, SAUR32, AhGI-13, AhGI-75, AhCUC3-12, and AhCUC3-65) were upregulated in the PAW treated condition at 6 days compared to the TW treated sprouts.

The Preventive Effects of Nanopowdered Peanut Sprout-added Caciocavallo Cheese on Collagen-induced Arthritic Mice

Dong-Hwi Kim,장윤혁,Hae Soo Kwak 한국축산식품학회 2014 한국축산식품학회지 Vol.34 No.1

The present study was carried out to investigate the effects of nanopowdered peanut sprout-added Caciocavallo cheese(NPCC) on the prevention and treatment of rheumatoid arthritis in DBA/IJ mice immunized with type II collagen. After theinduction of arthritis, the mice were being divided into five groups: (1) normal, no immunization; (2) CIA, collagen-inducedarthritis; (3) MTX, collagen-induced arthritis treated with methotrexate (0.3 mg/kg body weight); (4) CC, collagen-inducedarthritis treated with Caciocavallo cheese (0.6 g/d); (5) NPCC, collagen-induced arthritis treated with nanopowdered peanutsprout-added Caciocavallo cheese (0.6 g/d). Nanopowdered peanut sprout was ranged from 300 to 350 nm, while regularpowdered peanut sprouts were ranged from 50 to 150 µm. The NPCC group had considerable reductions of clinical scoresand paw thicknesses at the end of experiment as compared to the CIA group. In the serum analysis, the TNF-α, IL-1β, IL-6 and IgG1 levels in the NPCC group have decreased by 69.4, 75.9, 66.6, and 61.9%, respectively, when compared to theCIA group. The histological score and spleen index of the NPCC group were significantly lower than the CIA group. Inconclusion, the feeding NPCC method could delay and/or prevent the rheumatoid arthritis in the collagen-induced arthritismouse model. Based on this study, nanopowdered peanut sprouts could be applied to various functional cheeses.

청정 땅콩나물 생산을 위한 종자처리기술 확립

박은지 ( Eun Ji Park ),이규빈 ( Gyu Bin Lee ),허유 ( You Heo ),손병구 ( Beung Gu Son ),최영환 ( Young Whan Choi ),이용재 ( Yong Jae Lee ),박영훈 ( Young Hoon Park ),서정민 ( Jeong Min Suh ),강점순 ( Jum Soon Kang ) 한국환경과학회 2015 한국환경과학회지 Vol.24 No.6

The present study was conducted to develop seed treatment for the production of healthy and clean peanut sprout. Dry heat treatment of peanut seeds reduced the incidence of the rot. The seed treatment condition at 52℃ for 10 h. was the most efficient without inhibiting seed viability significantly. Seeds were dark cultured at 27℃ for up to 9 days. The treatment of Indole-B and gibberellic acid influenced germination, T50, fresh, dry weight, hypocotyl length, hypocotyl length diameter, root length, number of lateral root and epicotyl of peanut sprout. There were no differences in the germinability of peanut seeds between gibberellic acid treatment methods but higher fresh weight was observed in the GA3 solution spray after 2 hour water soaking. The general growth and lateral root development of peanut sprouts were suppressed by Indole-B which is used for inhibiting root formation and promoting hypocotyls. The treatment of gibberellic acid promoted hypocotyl elongation, but it did not influence on the growth of hypocotyls and root system.

Reduction of allergy eff ects of peanut sprout extract in a systemic anaphylaxis food allergy mouse model

Won-Kyung Yang,김동선,손은정,이윤미,Yang-Chun Park,Geung-Joo Lee,Seung Hyung kim 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.4

Peanut allergy is a major cause of food-induced anaphylaxis and subsequent fatality. Food allergies are becoming an increasingly important global health issue. This study was undertaken to verify the effects of peanut sprout extract (PNSE) on the inhibition of allergic and anaphylactic responses using a peanut (PN)-immunized food allergy mouse model. Fresh peanut sprouts were germinated for 0, 3, 5, and 7 days using mature peanuts. Mice were then sensitized to cholera toxin plus PNE or PNSE by intragastric administration on days 0 and 7, and were then challenged with PNE or PNSE on days 21 and 35. After 5 weeks, we examined the mucosal mast cell degranulation, ear swelling, and systemic anaphylaxis stimulated by PNE extract, in comparison with PNSE. Subsequently, Ara h1, a biomarker of PNE allergy; serum levels; and Th1/Th2 cytokine production in supernatants of cultured splenocytes were measured. PNSE treatment significantly attenuated the secretion of anti-Ara h1 antibody, mucosal mast cell degranulation, degree of systemic anaphylaxis, and ear swelling and increased the production of IFN-γ and IL-10, with a decrease in IL-4 secretion. The results of this study show that the allergenicity of PNE could be reduced by germination, which caused downregulation of Th2 lymphocyte activity, systemic anaphylactic response, and mast cell-mediated ear swelling in PNE-sensitized mice.

Ethanol Extract of Peanut Sprout Exhibits a Potent Anti-Inflammatory Activity in Both an Oxazolone-Induced Contact Dermatitis Mouse Model and Compound 48/80-Treated HaCaT Cells

( Da In Choi ),( Jee Young Choi ),( Young Jee Kim ),( Jee Bum Lee ),( Sun Ouck Kim ),( Hyong Taek Shin ),( Seung Chul Lee ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.2

Background: We developed an ethanol extract of peanut sprouts (EPS), a peanut sprout-derived natural product, which contains a high level of trans-resveratrol (176.75 μg/ml) and was shown to have potent antioxidant activity. Objective: We evaluated the potential anti-inflammatory activity of EPS by measuring its antioxidant potential in skin. Methods: The anti-inflammatory activity of EPS was tested using two models of skin inflammation: oxazolone (OX)-induced contact dermatitis in mice and compound 48/80-treated HaCaT cells. As biomarkers of skin inflammation, cyclooxygenase-2 (COX-2) and nerve growth factor (NGF) levels were measured. Results: OX-induced contact dermatitis was suppressed markedly in mice that were treated with an ointment containing 5% EPS as evidenced by a decrease in the extent of scaling and thickening (p<0.05) and supported by a histological study. COX-2 (messenger RNA [mRNA] and protein) and NGF (mRNA) levels, which were upregulated in the skin of OX-treated mice, were suppressed markedly in the skin of OX+EPS-treated mice. Consistent with this, compound 48/80-induced expression of COX-2 (mRNA and protein) and NGF (mRNA) in HaCaT cells were suppressed by EPS treatment in a dose-dependent manner. As an inhibitor of NF-κB, IκB protein levels were dose-dependently upregulated by EPS. Fluorescence-activated cell sorting (FACS) analysis revealed that EPS scavenged compound 48/80-induced reactive oxygen species (ROS) in HaCaT cells. Conclusion: EPS exerts a potent anti-inflammatory activity via its antioxidant activity in both mouse skin and compound 48/80-treated HaCaT cells in vitro. Compound 48/80-treated HaCaT cells are a useful new in vitro model of skin inflammation.(Ann Dermatol 27(2) 142∼151, 2015)