Effects of Temperature on Leaf Emergence Rates and Phyllochron of Naked and Malting Barley

Kang, Young-Kil,Ko, Koan-Su,Kang, Bong-Kyoon The Korean Society of Crop Science 1993 Korean journal of crop science Vol.38 No.5

온도가 쌀보리와 맥주보리의 출엽속도와 출엽간격에 미치는 영향을 구명하고자 쌀보리 3품종(능쌀보리, 새쌀보리, 향천과 001)과 맥주보리 3품종(두산 8호, 사천006, 진광보리)을 향온 7수준(4, 8, 12, 16, 20, 24, 28$^{\circ}C$)과 변온 7수준[6/2(명기 / 암기), 10/6, 14/10, 18/14, 22/18, 26/22, 30/$25^{\circ}C$]으로 유지시킨 생장상에서 4엽기까지 키우면서 매일 주간엽수를 조사하여 출엽속도 및 출엽간격를 산출한 결과를 요약하면 다음과 같다. 항온과 변온에 관계없이 공시품종 모두 동일 온도내에 있어서는 출아후 일수가 증가됨에 따라 주간 출엽수도 직선적으로 증가되었다. 평균기온 28$^{\circ}C$를 제외하고는 출아속도와 출아간격이 항온과 변온간에 현저한 차이를 보이지 않았다. 출아속도와 출아간격은 품종간에 유의한 차이가 있었고 동일 품종내 온도간에도 현저한 차이가 있었다. 평균기온이 증가됨에 따라 일당 출아속도는 출아 최적온도까지 곡선적으로 증가된 다음 감소되었는데, 6품종의 출아 최적온도는 20.1~21.5$^{\circ}C$로 품종간 현저한 차이는 없었으나 출아 최적온도에서의 출엽속도는 쌀보리가 0.202~0.226엽/일, 맥주보리가 0.231~0.241엽/일으로 맥주보리가 큰 경향이었다. 평균이온이 증가됨에 따라 유효적산온도당 출엽속도는 지수함수적으로 감소되었고, 출엽간격(일엽당 적사온도)은 지수함수적인 증가를 보였는데, 6품종의 평균 출엽간격은 4$^{\circ}C$에서 46.2GDD/엽, 28$^{\circ}C$에서 129.3GDD/엽이었다. Three naked and three malting barley cultivars were grown at constant temperatures of 4, 8, 12, 16, 20, 24 and 28$^{\circ}C$, and day/night temperatures of 6/2, 10/6, 14/10, 18/14, 22/18, 26/22 and 30/26$^{\circ}C$ through the fourth leaf stage in growth chambers to determine the effects of the temperature on leaf emergence rate and phyllochron in naked and malting barley seedlings. The number of leaves per main stem was recorded daily from the first leaf stage to the fourth. At a given temperature, the emergence of new leaves was a linear function of time for all cultivars. There were no great differences in leaf emergence rate and phyllochron between constant and variable day/night temperature regimes except at 28$^{\circ}C$. Leaf emergence rate and phyllochron significantly differed among cultivars and among mean temperatures within cultivars. For all cultivars, leaf emergence rate per day increased parabolically with increasing mean air temperature until an optimum temperature was reached and then declined. There were no differences in the optimum temperatures for the leaf emergence rate per day among six cultivars, which ranged 20.1 to 21.5$^{\circ}C$. The leaf emergence rates at the optimum temperatures ranged 0.202 to 0.226 leaves / day for naked barley cultivars and 0.231 to 0.241 leaves / day for malting barley cultivars. As temperature increased, leaf emergence rate per GDD decreased exponentially and the phyllochron (GDD/leaf) increased exponentially. The mean of the phyllochron for six cultivars was 46.2 GDD at 4$^{\circ}C$ and 129.3 GDD at 28$^{\circ}C$. These results suggest that the temperature and cultivar effects must be considered for prediction of leaf development in barley.

온도가 쌀보리와 맥주보리의 출엽속도와 출엽간격에 미치는 영향

Young Kil Kang,Koan Su Ko,Bong Kyoon Kang 韓國作物學會 1993 한국작물학회지 Vol.38 No.5

온도가 쌀보리와 맥주보리의 출엽속도와 출엽간격에 미치는 영향을 구명하고자 쌀보리 3품종(능쌀보리, 새쌀보리, 향천과 001)과 맥주보리 3품종(두산 8호, 사천006, 진광보리)을 향온 7수준(4, 8, 12, 16, 20, 24, 28℃ )과 변온 7수준[6/2(명기 / 암기), 10/6, 14/10, 18/14, 22/18, 26/22, 30/25℃ ]으로 유지시킨 생장상에서 4엽기까지 키우면서 매일 주간엽수를 조사하여 출엽속도 및 출엽간격를 산출한 결과를 요약하면 다음과 같다. 항온과 변온에 관계없이 공시품종 모두 동일 온도내에 있어서는 출아후 일수가 증가됨에 따라 주간 출엽수도 직선적으로 증가되었다. 평균기온 28℃ 를 제외하고는 출아속도와 출아간격이 항온과 변온간에 현저한 차이를 보이지 않았다. 출아속도와 출아간격은 품종간에 유의한 차이가 있었고 동일 품종내 온도간에도 현저한 차이가 있었다. 평균기온이 증가됨에 따라 일당 출아속도는 출아 최적온도까지 곡선적으로 증가된 다음 감소되었는데, 6품종의 출아 최적온도는 20.1~21.5℃ 로 품종간 현저한 차이는 없었으나 출아 최적온도에서의 출엽속도는 쌀보리가 0.202~0.226엽/일, 맥주보리가 0.231~0.241엽/일으로 맥주보리가 큰 경향이었다. 평균이온이 증가됨에 따라 유효적산온도당 출엽속도는 지수함수적으로 감소되었고, 출엽간격(일엽당 적사온도)은 지수함수적인 증가를 보였는데, 6품종의 평균 출엽간격은 4℃ 에서 46.2GDD/엽, 28℃ 에서 129.3GDD/엽이었다.ose와 fructose가 초기에는 많았으나 후에는 상당히 적어졌다. 줄기는 glu-cose가 많고 기타는 흔적 정도 였으며 뿌리는 초기에 sucrose가 종자에서 보다 높았으나 그 후 모든 당이 거의 흔적뿐이었다.과 GA3 반응성을 조사한 결과, 조합내 개체들간에 간장의 분리를 인정할 수 없었고, GA3 처리에 무반응성을 보였다. 따라서 Fukei 71의 단간유전자 d50은 GA3 무반응성과 밀접한 연관이 있는 것으로 생각되었다.에서 G1 기는 길어졌고 G2 기와 M기는 짧아졌다. 그러나 서광벼에서는 각 phase의 상대적 기간이 거의 일정한 비율이었다. 3. DNA, RNA 및 protein 합성은 온도가 하항함에 따라 증가하는 경향이었다.에 의해서는 최고분얼기나 유수분화기에 처리하면 수당 영화수가 감소하였으나 감수분열기에 처리하면 수당 영화수가 변하지 않았다.장기였고 건구의 수량감수가 심한 시기는 수잉기였다. 따라 미개약 영화가 많은 품종과 개화 수분이 모두 이루어지지만 불임이 되는 품종도 볼 수 있었다. 5. 냉해의 유묘 검정 결과에서 관찰조사와 처리에 의한 생육 억제 정도와는 반드시 일치하지 않았으나 종합적으로 볼 때 제 002, 팔금, 수성 등은 냉해에 강하고 수원 213-1, 재건, 팔달, Shirogane, 팔굉, 만경 등은 약하였다. 6. 감수분열기 및 출수기 처리 시의 일심율과 유묘 시의 냉해 정도 및 생육 억제 정도 등의 상호 관계를 살펴본 즉 대체로 유의적 상관을 보이지 않았다.련성을 보였는데, 특히 전체적인 비만도를 평가하는 체중, 체질량지수, 비만지수의 증가는 심혈관계질환의 예측지표로 볼 수 있는 Three naked and three malting barley cultivars were grown at constant temperatures of 4, 8, 12, 16, 20, 24 and 28~circC , and day/night temperatures of 6/2, 10/6, 14/10, 18/14, 22/18, 26/22 and 30/26~circC through the fourth leaf stage in growth chambers to determine the effects of the temperature on leaf emergence rate and phyllochron in naked and malting barley seedlings. The number of leaves per main stem was recorded daily from the first leaf stage to the fourth. At a given temperature, the emergence of new leaves was a linear function of time for all cultivars. There were no great differences in leaf emergence rate and phyllochron between constant and variable day/night temperature regimes except at 28~circC . Leaf emergence rate and phyllochron significantly differed among cultivars and among mean temperatures within cultivars. For all cultivars, leaf emergence rate per day increased parabolically with increasing mean air temperature until an optimum temperature was reached and then declined. There were no differences in the optimum temperatures for the leaf emergence rate per day among six cultivars, which ranged 20.1 to 21.5~circC . The leaf emergence rates at the optimum temperatures ranged 0.202 to 0.226 leaves / day for naked barley cultivars and 0.231 to 0.241 leaves / day for malting barley cultivars. As temperature increased, leaf emergence rate per GDD decreased exponentially and the phyllochron (GDD/leaf) increased exponentially. The mean of the phyllochron for six cultivars was 46.2 GDD at 4~circC and 129.3 GDD at 28~circC . These results suggest that the temperature and cultivar effects must be considered for prediction of leaf development in barley.

모싯잎 가루를 첨가한 찰보리 찐빵의 항산화 활성, 이화학적 특성 및 관능적 품질 특성에 관한 연구

김지현,김세정,윤정미,Kim, Ji-Hyun,Kim, Se-Jung,Yun, Jung-Mi 한국식품영양학회 2014 韓國食品營養學會誌 Vol.27 No.2

본 연구에 의하면 보리와 모싯잎을 첨가하여 찐빵을 제조하여 관능검사와 기계적 측정을 확인하여 기능성 식품개발과 보리 이용의 효율성 증대를 모색하였다. 기능성 식품인 찰보리와 모싯잎 가루의 항산화성을 알아보기 위하여 모싯잎 가루를 첨가한 보리찐빵의 DPPH 라디컬 소거능, 총 폴리페놀 함량을 측정하여 항산화 활성을 알아보았다. 찐빵의 총 폴리페놀 함량의 결과는 모시 찰보리 찐빵은 찰보리 찐빵보다 높은 수치를 나타내었다. 이는 보리와 찰보리의 시너지 효과로 보인다. 색도 측정을 한 결과, L값은 밀찐빵이 가장 높았고, 찰보리 찐빵이 가장 낮았다. a값은 모시 찰보리 찐빵이 유의적으로 그룹간에서 가장 낮았고(p<0.05), 찰보리 찐빵이 가장 높았다. b값은 모시 찰보리 찐빵이 16.18로 가장 높았고, 찰보리 찐빵이 가장 낮았다. 물리적 특성을 측정한 결과, 밀찐빵에 비해 찰보리 찐빵과 모시 찰보리 찐빵 간에는 경도가 높고, 찰보리 찐빵과 모시 찰보리 찐빵 간에는 경도 차이는 유의적으로 나타나지 않았으며, 탄성력이 모시 찰보리 진빵이 높았고, 부착성이 월등이 낮음을 관찰할 수 있었다. 모싯잎을 첨가한 찰보리 찐빵의 관능검사 결과, 색, 향미, 맛, 촉촉함, 씹힘성, 전반적인 기호도를 알아보았다. 전반적인 기호도는 모시 찰보리 찐빵이 유의적으로 가장 높았으며, 찰보리 찐빵과 대조군(밀찐빵) 간에는 유의성이 없었다. 이상의 관능검사 결과를 보면 모싯잎을 첨가한 밀가루와 보리가루의 비율이 7:3인 찰보리 찐빵이 가장 높은 관능평가를 받은 것으로 나타났다. 결과적으로 모싯잎을 첨가한 찰보리 찐빵은 높은 항산화 활성뿐만 아니라, 품질 특성을 향상시켰고, 기호도를 증가시켰다. 본 연구는 이러한 기초자료를 바탕으로 찰보리와 모싯잎을 함께 첨가하여 사용하는 것이 식품의 품질 특성 및 기능성을 높이는데 공헌을 할 것으로 생각된다. Increased consumption of a polysaccharide, ${\beta}$-glucans, in foods may prevent health related problems such as cancer, cardiovascular diseases and diabetes. ${\beta}$-glucans is a fibrous polysaccharide having proven both functional and medicinal properties. Recently, the FDA recommends the consumption of oat or oat products containing a total of at least 3 grams of bea-glucans per day for health improvement. The content of ${\beta}$-glucans in barley was almost four times higher than that in oat. In this study, the physicochemical and sensory characteristics and biological properties of steamed barley bread added with ramie leaf powder was investigated. The study of sensory characteristics was performed using the Quantitative Descriptive Analysis (QDA). 30 panelists were selected among our university students. They then evaluated the different sensory characteristics, such as overall preference, color, flavor, chewiness, moistness and taste. The color and texture analyses were determined using a colorimeter and texture analyser, respectively. In the sensory, color and texture evaluation, barley bread with ramie leaf showed higher values than barley bread and wheat bread did. The physiological activities were investigated through the total phenol content and 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity. Hence, barley appears to be a suitable food resource for making bread. This study suggests that barley bread added with ramie leaf can be used as one of the processing methods in promoting the consumption of barley. It might also help with the improvement of barley food industry.

Protective effects of a polysaccharide BLE0 isolated from barley leaf on bone loss in ovariectomized mice

Hwang, Youn-Hwan,Ha, Hyunil,Kim, Rajeong,Cho, Chang-Won,Song, Young-Ran,Hong, Hee-Do,Kim, Taesoo Elsevier 2019 International journal of biological macromolecules Vol.123 No.-

<P><B>Abstract</B></P> <P>Potent anti-osteoporotic drugs have been developed over the past decades; however, the substantial need for treatments that can effectively and safely manage osteoporosis remains unmet. Barley leaf-derived products are one of best functional foods that can be used as nutritional supplements and detoxifiers in humans and are beneficial in improving bone disease. However, little information is available regarding the anti-osteoporotic effects of polysaccharides as the main component of barley leaf. This study aimed to clarify the beneficial effects of barley leaf (BLE0) polysaccharides on bone loss in ovariectomized mice and osteoclast differentiation in bone marrow-derived macrophages. BLE0 remarkably inhibited receptor activator of the nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation in a dose-dependent manner. It also blocked RANKL-induced activation of osteoclastogenic signals including ERK and p38 and the expression of nuclear factor of activated T cells cytoplasmic 1, as a master regulator of osteoclast differentiation, leading to decreased expression of osteoclast-specific marker genes such as Atp6v0d2, DC-STAMP and cathepsin K. Micro-computed tomography revealed that a seven-week oral administration of BLE0 dramatically improved ovariectomy-induced trabecular bone loss. Anti-osteoporotic effects were confirmed using morphometric analysis. Taken together, BLE0 is a bioactive polysaccharide; it mitigates estrogen deficiency-induced bone loss by directly inhibiting osteoclast differentiation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The crude polysaccharide BLE0 was isolated from barley leaf (<I>Hordeum vulgare</I> L.). </LI> <LI> We performed the first in vivo evaluation of the antiosteoporosis effect of BLE0. </LI> <LI> BLE0 protected ovariectomized (OVX) mice from trabecular microarchitecture deterioration. </LI> <LI> BLE0 suppressed osteoclast differentiation by inhibiting the activation of MAP kinases/NF-κB pathways of osteoclastogenesis. </LI> </UL> </P>

Effect of Light Emitting Diode Radiation on Antioxidant Activity of Barley Leaf

이나영,이미자,김양길,Jong-Chul Park,Hong-Kyu Park,Jae-Seong Choi,Jong-Nae Hyun,Kee-Jong Kim,박기훈,고재권,Jung-Gon Kim 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.6

Antioxidant activity of extracts of barley leaves cultivated by light emitting diode (LED) radiation such as red, far-red, blue, blue-red, green, yellow, and white light was investigated. After measuring length and weight of the leaves cultivated, barley leaves were extracted using 70% ethanol. The Hunter color value, total phenolic compounds, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salts (ABTS) radical-scavenging activities of extracts were determined. Lengths of samples cultivated by red and green light radiation were 13.7 and 13.6 cm, respectively. Hunter L* values of samples cultivated by red, far-red, and UVA radiation were 65.29, 67.55, and 67.57, respectively. The content of total phenolic compounds of samples cultivated by blue light radiation was 1.62 mg/L of sample. The DPPH radical-scavenging activities of samples cultivated by blue, green, UVA, and white light radiation were 64.28, 48.92, 55.95, and 48.72%, respectively. The ABTS radical-scavenging activity of samples cultivated by blue light radiation scored higher compared with those of samples cultivated with other LED lights. Antioxidant activities of barley leaves showed different results depending on harvest time. Application of LED radiation during re-cultivation after the first harvest showed increasing tendency on antioxidant activity of barley leaves.

Effect of Light Emitting Diode Radiation on Antioxidant Activity of Barley Leaf

Lee, Na-Young,Lee, Mi-Ja,Kim, Yang-Kil,Park, Jong-Chul,Park, Hong-Kyu,Choi, Jae-Seong,Hyun, Jong-Nae,Kim, Kee-Jong,Park, Ki-Hun,Ko, Jae-Kwon,Kim, Jung-Gon The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.6

Antioxidant activity of extracts of barley leaves cultivated by light emitting diode (LED) radiation such as red, far-red, blue, blue-red, green, yellow, and white light was investigated. After measuring length and weight of the leaves cultivated, barley leaves were extracted using 70% ethanol. The Hunter color value, total phenolic compounds, and 1,1-diphenyl-2-picrylhydrazyl (OPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salts (ARTS) radical-scavenging activities of extracts were determined. Lengths of samples cultivated by red and green light radiation were 13.7 and 13.6 cm, respectively. Hunter $L^*$ values of samples cultivated by red, far-red, and UVA radiation were 65.29, 67.55, and 67.57, respectively. The content of total phenolic compounds of samples cultivated by blue light radiation was 1.62 mg/L of sample. The OPPH radical-scavenging activities of samples cultivated by blue, green, UVA, and white light radiation were 64.28, 48.92, 55.95, and 48.72%, respectively. The ARTS radical-scavenging activity of samples cultivated by blue light radiation scored higher compared with those of samples cultivated with other LED lights. Antioxidant activities of barley leaves showed different results depending on harvest time. Application of LED radiation during re-cultivation after the first harvest showed increasing tendency on antioxidant activity of barley leaves.

Structure elucidation of an immunostimulatory arabinoxylan-type polysaccharide prepared from young barley leaves (<i>Hordeum vulgare</i> L.)

Kim, Hoon,Hong, Hee-Do,Shin, Kwang-Soon Applied Science Publishers 2017 Carbohydrate Polymers Vol.157 No.-

<P><B>Abstract</B></P> <P>We recently isolated an immunostimulating polysaccharide (BLE-P-I), which possesses a large proportion of arabinoxylan, from young barley leaves. In the present study, to elucidate the structural details of BLE-P-I, it was fractionated into enzyme-resistant fraction (BLE-P-I-X1) and arabinoxylan-rich oligosaccharide fraction (BLE-P-I-X2) after <I>endo</I>-xylanase hydrolysis. Commercial wheat arabinoxylan was also fractionated into WAX-XI and WAX-X2 after the same treatment. BLE-P-I-X2 consisted of arabinose and xylose, including 11 types of neutral glycosidic linkages, whereas WAX-X2 had less, with 6 types of linkages. Mass spectrometric results indicated that WAX-X2 was composed of a xylan backbone bearing only arabinose. In contrast, BLE-P-I-X2 consisted of a xylan backbone with acetate, arabinose, galactose, glucuronic acid, and 4-<I>O</I>-methylglucuronic acid, resembling structural characteristics as glucuronoarabinoxylan. Macrophage-stimulatory activity showed that BLE-P-I-X2 has a role in the expression of the activity. These results indicate that the structural complexity of arabinoxylan seems to be responsible for the immunomodulatory activity in barley leaf.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Immunostimulating polysaccharide (BLE-P-I) was purified from young barley leaf. </LI> <LI> BLE-P-I was further fractionated into two fractions by <I>endo</I>-xylanase digestion. </LI> <LI> Arabinoxylan-rich oligosaccharide (BLE-P-I-X2) was released by xylanase catalysis. </LI> <LI> Macrophage activity was compared between before and after xylanase treatment. </LI> <LI> BLE-P-I-X2 was identified by diverse analytical method and technique. </LI> </UL> </P>

Alternative Measure for Assessing Incidence of Leaf Stripe on Barley

Arabi, Mohammad Imad Eddin,Jawhar, Mohammad The Korean Society of Plant Pathology 2012 Plant Pathology Journal Vol.28 No.2

Pyrenophora graminea, the causal agent of leaf stripe disease, is an economically important pathogen of barley found worldwide. It is critical to clearly define and standardize the leaf stripe assessment methods to avoid subjectivity and variability between assessors. Therefore, in this study, a comparison between the proportion of diseased plants (DP) and the proportion of diseased leaves (DL) per plant was investigated. Disease assessments were made visually at multiple sample sites in artificially and naturally inoculated research and production fields during four growing seasons. There were significant differences (P = 0.001) among cultivars in mean DP and DL averages, which are consistently higher in susceptible barley. However, DP values increased linearly as DL increased. The slopes and intercepts of the DP-DL relationship were consistent over the four growing seasons. This result might make a significant contribution for leaf stripe assessment in barley breeding programme.

Alternative Measure for Assessing Incidence of Leaf Stripe on Barley

Mohammad Imad Eddin Arabi,Mohammad Jawhar 한국식물병리학회 2012 Plant Pathology Journal Vol.28 No.2

Pyrenophora graminea, the causal agent of leaf stripe disease, is an economically important pathogen of barley found worldwide. It is critical to clearly define and standardize the leaf stripe assessment methods to avoid subjectivity and variability between assessors. Therefore, in this study, a comparison between the proportion of diseased plants (DP) and the proportion of diseased leaves (DL) per plant was investigated. Disease assessments were made visually at multiple sample sites in artificially and naturally inoculated research and production fields during four growing seasons. There were significant differences (P = 0.001) among cultivars in mean DP and DL averages, which are consistently higher in susceptible barley. However, DP values increased linearly as DL increased. The slopes and intercepts of the DP-DL relationship were consistent over the four growing seasons. This result might make a significant contribution for leaf stripe assessment in barley breeding programme.

당뇨 쥐에서 상엽추출물을 첨가한 보리면의 혈당조절효과

박충무,윤현서 한국융합학회 2018 한국융합학회논문지 Vol.9 No.8

본 연구는 상엽추출물이 포함된 보리면의 혈당조절능력을 입증하기 위하여 4주간 고지방/고탄수화물 식이 후 streptozotocin과 nicotinamide로 당뇨를 유발하고 음성대조군, 양성대조군, 보리면대조군, 상엽대조군, 보리면+상엽추출물 2.5%군, 보리면+상엽추출물 5%군의 6군으로 나누었다. 그 후 2주간 식이를 진행하며 체중, 혈당, 간 기능 및 혈액 내 지질의 변화를 측정하였다. 그 결과 보리면과 상엽 단독 섭취군보다 혼합섭취군에서 체중 감소가 적었고 혈당조절효과도 뛰어났으 며 이는 경구 당 부하검사에서도 동일한 결과를 보였다. 또한 간기능, 혈중 지질 개선 효과도 혼합 섭취군에서 강하게 나타났 다. 따라서, 보리면과 상엽추출물을 함유한 식이는 복합식이에서 단독식이보다 강한 혈당조절효과와 간 기능 개선, 지질 강 하효과도 나타났다. 결과적으로 상엽을 포함하는 보리면은 혈당을 조절하고, 간 기능, 지질 강하 기능을 보여 만성질환 관리 에도 도움이 될 것으로 사료된다. This study aimed to investigate the blood glucose regulatory effect of barley noodles contained mulberry leaves water extract (MLWE) in diabetic mice in order to provide fundamental data as a functional food. ICR mice were divided into six groups as follows: normal control, positive control, barley control, mulberry control, barley+MLWE 2.5% and barley+MLWE 5% groups. High fat/high carbohydrate diet was fed for 4 weeks and diabetes mellitus was induced by i.p. injection of streptozotocin and nicotinamide. After diabetes induction, experimental diet was supplemented for 2 weeks and simultaneous measurement of body weight, blood glucose (including glucose tolerance test), liver function and blood lipid profile was conducted. As a result, there was less decrease in body weight compared to the positive control group in the groups that supplemented barley and MLWE together than the group ingesting barley and MLWE alone. In addition, regulatory effects on blood glucose, liver function and blood lipid concentration were also stronger in barley and MLWE concurrently supplemented groups. Consequently, combination of barley and MLWE was effective in the regulation of body weight, blood glucose, liver function, and blood lipid in diabetic mice. Therefore, this recipe could be a promising strategy for beneficial effects in the patients of diabetes mellitus.