HaCaT 세포에서 며느리 배꼽 추출물의 AP-1, PI3K/Akt 및 MAPK 활성 조절을 통한 광손상 억제 효과

윤현서,박충무 대한통합의학회 2024 대한통합의학회지 Vol.12 No.1

Purpose : Skin is the primary barrier to protect the body from various exogenous factors. Among them, UVB exposure can cause the induction of not only excessive inflammatory responses but also the degradation of extracellular matrix (ECM), including collagen and elastin. This study tried to investigate the ameliorative effect of Persicaria perfoliata ethanol extract (PPEE) on UVB-irradiated photodamage through the regulation of activator protein (AP)-1, phosphoinositide 3-kinase (PI3K)/Akt, and mitogen-activated protein kinase (MAPK) signaling molecules in HaCaT cells. Methods : The cytotoxicity of PPEE on HaCaT cells was evaluated by the WST-1 assay. The 80 mJ/㎠ of UVB (312 ㎚) was irradiated on HaCaT cells to induce the photodamage. Western blot analysis was conducted to investigate the protein expression levels of cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9, and heme oxygenase (HO)-1 for ameliorative status by PPEE treatment in UVB-exposed HaCaT cells. In addition, the activated status of the inflammatory transcription factor, AP-1, as well as upstream signaling molecules, PI3K/Akt, and MAPK, were also evaluated by Western blot analysis. Results : Any cytotoxic effect was not induced at the concentration up to 200 ㎍/㎖ by PPEE treatment. Protein expression levels of COX-2 and MMP-9 were significantly down- and up-regulated by PPEE treatment. The inflammatory transcription factor AP-1, stimulated by UVB irradiation, was also significantly attenuated by PPEE treatment. The phosphorylated status of PI3K/Akt and MAPK were mitigated by PPEE treatment in UVB-exposed HaCaT cells. Moreover, PPEE treatment potently accelerated the expression of HO-1 and its transcription factor, nuclear factor-erythroid 2-related factor (Nrf)2, which is known for its anti-inflammatory activity. Conclusion : Consequently, PPEE treatment significantly regulated COX-2 and MMP-9 expressions in UVB-irradiated HaCaT cells. The inflammatory transcription factor AP-1, along with upstream signaling molecules PI3K/Akt and MAPKs, were also attenuated by PPEE treatment in UVB-exposed HaCaT cells. Additionally, PPEE treatment exaggerated HO-1 expression and Nrf2 activation, which might have contributed to the anti-inflammatory activity of PPEE. These results indicate that PPEE could be a candidate for attenuating UVB-induced photodamage in human skin.

시 지역과 군 지역주민들의 주관적 구강건강인식과 삶의 질 (2015년 지역사회건강조사자료 이용)

윤현서,Yoon, Hyunseo 대한통합의학회 2017 대한통합의학회지 Vol.5 No.3

Purpose : The purpose of this study was to examine the self-rated oral health status of local residents and their quality of life in urban and county areas in an effort to provide information on how to improve their quality of life. Method : The 2015 community health survey data for South Gyeongsang Province(10 urban regions and 10 county areas) were analyzed. Result : The findings of the study were as follows: As for the characteristics of self- rated oral health, there were better self-rated oral health, less chewing difficulty, less use of dentures, higher rate of receiving dental checkups and more scaling experience in the urban communities than in the county areas. Concerning EQ-5D and happiness index by region, the two were higher in the urban regions than in the county areas. In regard to EQ-5D and happiness index by the characteristics of self-rated oral health, better self-rated oral health status and less chewing difficulty led to higher EQ-5D and higher happiness index. And the two were higher when dentures were not used, when more dental checkups were received and when there was more scaling experience. Conclusion : Therefore in order to boost the quality of life of local residents, the preparation of various educational programs is necessary to raise their awareness of health, and they should be provided with a wide range of medical benefits by dispersing medical institutions that are mostly located in urban communities or by expanding public health services in county areas.

천연추출물을 함유한 세치제의 치아미백효능에 대한 임상적 실험 연구

윤현서,김혜진 한국치위생학회 2018 한국치위생학회지 Vol.18 No.1

Objectives: Despite a rise of an interest in tooth whitening, diverse problems are being caused in case of hydrogen peroxide that is being used as a tooth bleaching agent. Thus, the aim was to examine tooth whitening effectiveness using natural products as a plan for supplementing this. Methods: As a result of having measured a tooth color through using VITA Easyshade V after having developed toothpaste with the application of extracts such as Citrus Peels, Mulberry (Morus alba L.) Root Bark, strawberry, and lemon, and then having used it for 10 weeks, they are as follows. Both upper and lower 6 anteriors mostly got brighter. Results: A statistically significant difference was shown especially in the right canine (p=0.015), in the right central incisor (p=0.007), and in the left central incisor (p<0.001). In consequence of having measured a color change, the tooth got brighter gradually in the higher extract content and in the lengthier use time. In case of canine, it got less bright compared to other teeth. In the outcome of evaluating sensuality, most of the questions were indicated to be high in case of using a whitening toothpaste for 10 weeks. But in what “there is no stickiness in the mouth, the stickiness was more felt in the use up to 5 weeks, but was improved in 10 weeks. Conclusions: The bleaching effectiveness was proved by developing a toothpaste with the application of natural extracts. A short-term effect cannot be seen like a whitening agent of using hydrogen peroxide. But there is a continuous effect in consideration of tooth-brushing more than 3 times a day.

Enhanced Antioxidative Potential by Silymarin Treatment through the Induction of Nrf2/MAPK Mediated HO-1 Signaling Pathway in RAW 264.7 Cells

윤현서,안현,박충무 한국생명과학회 2023 생명과학회지 Vol.33 No.10

Silymarin, which is derived from dried Silybum marianum (milk thistle) seeds and fruits, possesses various beneficial properties, such as hepatoprotective, antioxidative, anti-inflammatory, and anticancer activity. This research aimed to explore the antioxidative activity of silymarin against oxidative stress and understand its molecular mechanism in RAW 264.7 cells. The study employed cell viability and reactive oxygen species (ROS) formation assays and western blot analysis. The results demonstrated that silymarin effectively reduced intracellular ROS levels induced by lipopolysaccharide (LPS) in a dose-dependent manner without causing any cytotoxic effects. Moreover, silymarin treatment significantly upregulated the expression of heme oxygenase (HO)-1, a phase II enzyme known for its potent antioxidative activity. Additionally, silymarin treatment significantly induced the expression of nuclear factor-erythroid 2 p45-related factor (Nrf) 2, a transcription factor responsible for regulating antioxidative enzymes, which was consistent with the upregulated HO-1 expression. To investigate the involvement of key signaling pathways in maintaining cellular redox homeostasis against oxidative stress, the phosphorylation status of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) was estimated by western blot analysis. The results showed that silymarin potently induced HO-1 expression, which was mediated by the phosphorylation of p38 MAPK. To further validate the antioxidative potential of silymarin-induced HO-1 expression, tert-butyl hydroperoxide (t-BHP)-induced oxidative damage was employed and attenuated by silymarin treatment, as identified by a selective inhibitor for each signaling molecule. In conclusion, silymarin robustly enhanced antioxidative activity by inducing HO-1 via the Nrf2/p38 MAPK signaling pathway in RAW 264.7 cells.

Huh7 간암세포에서 민들레 추출물의 상피간엽전환 억제를 통한 항전이 효과

윤현서,안현,박충무 대한통합의학회 2023 대한통합의학회지 Vol.11 No.3

Purpose : Epithelial‐to‐mesenchymal transition (EMT) is recognized as an important cellular response in metastatic proceduresand characterized by loss of cellular polarity as well as gain of mesenchymal features, which enables migration and invasion. Hepatocellular carcinoma (HCC) is one of the most common primary carcinomas in the liver and exhibits a poor prognosis due to frequent extrahepatic metastasis. Taraxacum officinale has been used for a long time in oriental medicine because of its various pharmacological activitiessuch as anti-rheumatic, anti-inflammatory, antioxidative, and anticarcinogenic activities. In this study, the anti-metastatic activity of T. officinale water extract (TOWE) and ethanol extract (TOEE) was investigated through the regulation of EMT in the Huh7 cells. Methods : The effects of TOWE and TOEE on migratory and invasive activities were investigated by wound healing and in vitro invasion assays. Western blot analysis was also applied to analyze protein expression levels associated with EMT and their upstream transcription factors in Huh7 cells. Results : TOWE and TOEE treatment potently inhibited migration and invasion of Huh7 cells compared to the untreated group. Both extracts treatment inhibited protein expression levels of N-cadherin, matrix metalloproteinase (MMP)-9, and vimentin while E-cadherin was significantly accelerated. In addition, the activated status of transcription factors, Snail, nuclear factor (NF)-κB, and zinc finger E-box binding homeobox (ZEB)1 was also inhibited with statistical significance. In comparison to both extracts, TOEE more potently attenuated migration, invasion, and EMT markers as well as their transcription factors in Huh7 cells than TOWE, which means that TOEE might possess more functional phytochemicals than TOWE. Conclusion : Consequently, TOWE and TOEEattenuated metastatic activity of hepatocellular carcinoma through the regulation of EMT markers and their transcription factors in Huh7 cells, which means that T. officinale might be a promising strategy for a chemopreventive agent against HCC metastasis.

보육교사의 구강보건교육 및 칫솔질 지도에 따른 유아의 치면세균막 지수(QHI) 변화

윤현서,배수명,김혜진 국제차세대융합기술학회 2022 차세대융합기술학회논문지 Vol.6 No.1

본 연구는 보육교사의 주도적인 칫솔질 지도(칫솔질 교육, 칫솔질 지도)를 통해 유아의 치면세균막지수(Qugley-Hein indx) 변화를 살펴보고 구강건강 증진 방안을 모색하고자 시행하였다. 2020년 9월 8일부터 2021년 2월 18일까지 B시 D대학교 부설 어린이집 보육교사 4명과 원아 36명을 대상으로 보육교사의 주기적인 구강보건교육과 매일 점심 후 칫솔질 지도를 통해 치면세균막지수(Qugley-Hein indx)가 감소하는 것을 확인하였다. 전문가(치과위생사)의 보육교사 대상 구강보건교육으로 유아의 구강건강관리에 대한 관심과 필요성이 높아지는 긍정적인 효과를 확인하였으며 유아의 점심식사 후 칫솔질 지도를 통해 구강질환을 예방할 수 있는 긍정적인 효과를 확인하였다. 이를 통해 유아를 대상으로 구강관리를 좀 더 효율적으로 할 수 있는 보육교사의 교육 기회를 확대하고 관심을 유도할 수 방안이 모색되어져야 할 것이다. This study was conducted to examine changes in the Qugley-Hein indx of infants through the guidance of childcare teachers (tooth brushing education, brushing instruction) and to seek ways to improve oral health. From September 8, 2020 to February 18, 2021, the dental plaque index (Qugley-Hein indx) was confirmed to decrease. Oral health education for childcare teachers by experts (dental hygienists) confirmed the positive effect of increasing children's interest and need for oral health care. Confirmed. Through this, it is necessary to find ways to expand educational opportunities for childcare teachers who can do oral care more effectively for children and to induce interest.

창원시와 함안군 지역주민들의 주관적 구강건강인식수준 조사

윤현서,Yoon, Hyun-Seo 한국학교ㆍ지역보건교육학회 2018 한국학교지역보건교육학회지 Vol.19 No.1

The purpose of this study was to examine the levels of the subjective oral health awareness of local residents in the city of Changwon and Haman county in an effort to lay the foundation for the development of customized local oral health education programs. The 2015 community health survey data were used, and the data from 864 residents in Changwon and from 719 dwellers in Haman county were analyzed. The Changwon residents brushed their teeth after lunch more, received more oral checkups over the past year and had more scaling experience. The Haman residents had more experience of not receiving dental treatment. The subjective health awareness, subjective oral health awareness, chewing difficulty and periodontal health of the Haman dwellers were all worse, and all the levels of awareness were lower among the respondents who were older and who were diagnosed with hypertension and/or diabetes. The respondents who made use of dentures and who didn't brush their teeth after lunch had experience of not receiving dental treatment, and all the levels of health-related awareness were lower among the respondents who didn't receive dental checkups nor had scaling experience. To remedy the situation, efforts should be made to prevent and manage chronic diseases, and the establishment of a system that guarantees regular dental checkups and scaling is required. And the kind of environments that everybody can receive dental treatment when they want should be prepared. In order to change negative awareness, necessary measures should be taken to provide oral health education that is not temporary but sustainable and tailored to local characteristics and different life stages.

산약(Disocorea batatas) 에탄올추출물의 L. gasseri, S. mutans, P. gingivalis에 대한 항균능과 성장억제 효과

윤현서,박충무 대한통합의학회 2023 대한통합의학회지 Vol.11 No.2

Purpose : In this study, to prove the antibacterial effect of Disocorea batatas, which is widely used for food, and to confirm the growth inhibitory effect, the antibacterial activity against L. gasseri, S. mutans, and P. gingivalis was verified. Based on this, it is intended to verify the utility as a preventive and therapeutic composition for dental caries and periodontal disease. Methods : RAW 264.7 cells were used to verify the cell survival rate and NO (Nitric Oxide) inhibitory effect on Disocorea batatas ethanol extract (DBEE). In order to verify the antibacterial effect against L. gasseri, S. mutans, and P. gingivalis, concentrations of 125, 250, and 500 ㎎/㎖ of DBEE were used and measured by the disk diffusion method. In order to confirm the growth inhibitory effect, the absorbance was measured at 600 ㎚ at 3, 6, 12, 18, and 24 hours using the liquid medium dilution method, and the growth inhibitory effect was measured compared to the control group. Results : The cell viability for DBEE was 91 % at 50 ㎎/㎖, and there was no cytotoxicity. The NO production inhibitory effect was shown from 10 μg/ml, and the higher the concentration, the greater the inhibitory effect. As for the antimicrobial effect using the disk diffusion method, the higher the concentration, the higher the antibacterial effect. At 125 ㎎/㎖ and 250 ㎎/㎖, S. mutans and L. gasseri showed high antimicrobial activity, and at 500 ㎎/㎖, the antibacterial effect was higher in L. gasseri. The growth inhibitory effect in DBEE was concentration-dependent as the higher the concentration, the higher the growth inhibitory effect, and all of them began to show growth inhibitory effects from 6 hours. Conclusion : Considering that it is widely used as an edible and medicinal material, Disocorea batatas has shown the potential to be used as a substance to prevent and alleviate dental caries and periodontal diseases, and it is believed that further research can be applied to oral health care products.

Antibacterial activity of Dioscorea batatas, Morus alba, and Tagetes erecta against E. coli and S. aureus

윤현서 대한의생명과학회 2023 Biomedical Science Letters Vol.29 No.3

In this study, the anti-bacterial activity of three medicinal herbs such as Dioscorea batatas, Morus alba, and Tagetes erecta, have been applied in oriental medicine against Escherichia coli and Staphylococcus aureus. The antibacterial activity of Dioscorea batatas ethanol extract (DBEE), Morus alba ethanol extract (MAEE), and Tagetes erecta ethanol extract (TEEE) was analyzed by disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. As a result, three medicinal herbs exhibited antibacterial activity in a dose-dependent manner as well as MAEE and TEEE showed the most potent antibacterial activity followed by disk diffusion assay against E. coli and S. aureus, respectively. MIC against E. coli and S. aureus was 1.6 mg/mL of MAEE and TEEE as well as 0.2 mg/mL of TEEE. MBC against both strains was 5 mg/mL of DBEE and 2.5 mg/mL of TEEE. Consequently, three medicinal herbs in this study showed potent antibacterial activity through the inhibited growth of E. coli and S. aureus, which is considered as a potential candidate for cosmeceutic agents to attenuate the pruritus. In addition, antibacterial and antihistamine effects followed by the extraction solvents should be analyzed in future studies.

지역주민들의 보건교육(흡연, 음주, 영양)경험 유무에 따른 주관적 건강인식수준과 주관적 행복감

윤현서,박충무,전진호 대한통합의학회 2018 대한통합의학회지 Vol.6 No.4

Purpose : This study is based on the purpose of expanding health education to improve the subjective health of local residents and to improve their sense of happiness by identifying the relationship between health education experience and happiness. Method : T-test, one-way anova and correlation analysis were conducted in order to identify the levels of subjective health recognition and happiness followed by health education experience based on 2017 the Community Health Survey of Gyeongnam province. Result : The experience rate on non-smoking education, drinking education and nutrition education was high in all in case of dwelling in city area, of the lower age, of doing economic activity, of the high academic background, and of high income level. The subjective well-being was high in case of residing in county area, of the low age, of high terminal academic background, of doing economic activity, and of high income level. As for the subjective health awareness and the self-perceived oral health status, the recognition level was high in all in case of having the experience of non-smoking, drinking and nutrition educations. The subjective well-being was high in case of having the experience of non-smoking, drinking and nutrition educations. Conclusion : As a result of this study, the educations of targeting local residents are helpful even for enhancing quality of life in local residents without remaining in simply acquiring knowledge. Hence, arranging a place of public health education with diverse topics and expanding subjects are considered to be likely useful for upgrading local residents' quality of life. 본 연구는 2017년 경상남도 20개 시군을 대상으로 조사되어진 지역사회건강조사자료를 바탕으로 하여 지역주민들의 교육경험에 따른 주관적 건강인식수준과 주관적 행복감을 분석하여, 교육의 기회확대를 통한 건강에 대한 인식개선과 삶의 질 증진을 위한 기초자료를 제공하고자한다. 금연교육, 음주교육, 영양교육에 대한 경험률은 모두 시 지역에 거주고하고, 연령이 낮을수록, 경제활동을 하고, 학력이 높고, 소득수준이 높은 경우 높았다. 주관적 행복감은 군 지역에 거주하고, 남자, 연령이 낮고, 최종학력이 높으며, 경제활동을 하고 소득수준이 높은 경우 행복감이 높았다. 주관적 건강인식과 주관적 구강건강인식에서는 금연, 음주, 영양교육 경험이 있는 경우 모두 인식수준이 높았다. 금연, 음주, 영양교육경험이 있는 경우 주관적 행복감이 높았다. 따라서 본 연구결과 지역주민을 대상으로 한 교육들은 단순한 지식습득에서 그치지 않고 지역주민들의 삶의 질 향상에도 도움이 되므로 다양한 주제로 보건교육의 장을 마련하고 대상을 확대한다면 지역주민들의 삶의 질 향상에 도움이 되리라 사료된다.