생약복합제제의 병용투여에 관한 연구 (제1보) : 가미청심연자탕과 항고혈압약물과의 병용투여 Combined usage of Kamichungsimyunja-Tang and Anti-hypertensive Drugs

이석주,김남재,김종우,홍남두 한국병원약사회 1993 병원약사회지 Vol.10 No.4

Effects of Combined usage of combined preparation of crude drugs(Kamichungsimyunja-Tang) and anti-hypertensive drugs were examined in anti-hypertensive effects in rats and diuretic effects in mice. Kamichungsimyunja-Tang is an added and subtracted prescription of Chungsimyunja-Tang which it has become one of the favorate prescriptions at the Oriental Medical Hospital, Kyung-Hee University. Kamichungsimyunja-Tang has been used widely in cardiovascular disorder in Oriental medicines. Kamichungsimyunja-Tang was orally given and anti-hypertensive drugs such as hydralazine, verapamil and atenolol were intravenously given to rats. Effects of combined usage of Kamichungsimyunja-Tang and antihypertensive drugs for the anti-hypertensive actions significantly increased the anti-hypertensive effect of anti-hypertensive drugs. Kamichungsimyunja-Tang and diuretic drugs such as furosemide and hydrochlorothiazide was orally given to mice. Effects of combined usage of Kamichungsimyunja-Tang and diuretic drugs for the diuretic effects significantly increased the effect of diuretic drugs. These result suggested that combined usage of Kamichungsimyunja-Tang and anti-hypertensive drugs and diuretic drugs for the anti-hypertensive and diuretic actions significantly increased the effect of anti-hypertensive and diuretic drugs.

Anti-atherosclerotic effect of herbal extracts in N(G)-nitro-L-arginine methyl ester-treated rats

Senthil Nagarajan,Rangachari Balamurugan,신은주,심규석,김민정,이정준,이재권 한국응용생명화학회 2019 Journal of Applied Biological Chemistry (J. Appl. Vol.62 No.3

This study aimed to evaluate the anti-atherosclerotic and anti-hypertensive effects of six different plant extracts using a N(G)-nitro-L-arginine-methyl ester (L-NAME)-induced rat model of hypertension. All extracts were administered orally for six weeks. At the end of the study period blood pressure, blood flow, aortic histopathology, and hepatic endothelial nitric oxide synthase (eNOS) expression were measured. Subsequently, we also measured the levels of intracellular reactive oxygen species, nitric oxide (NO), and anti-inflammatory cytokines in vitro. Based on these screening results, we selected extracts of Cinnamomum cassia (C. cassia) and Salvia miltiorrhiza (S. miltiorrhiza) for further evaluation. C. cassia and S. miltiorrhiza extracts ameliorated hypertension and atherosclerosis in L-NAME-treated rats in a dose-dependent manner. In addition, a mixture of C. cassia and S. miltiorrhiza had an additive effect to reduce blood pressure, increase blood flow, and normalize aortic tissue. This mixture demonstrated antioxidative and anti-inflammatory activities in vitro. In conclusion, although further analysis of the therapeutic mechanism is required, the anti-hypertensive and anti-atherosclerotic effects of this mixture are likely mediated by increased eNOS expression, and its anti-oxidative and anti-inflammatory activities.

Effective screening for the anti-hypertensive of selected herbs used in the traditional Korean medicines

김지연,김도람,권오란 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.4

In this study, Korean medicinal herb extracts were investigated for their in vitro effect on anti-hypertensive activities, including nitric oxide (NO) production in the human umbilical vein endothelial cell line EA.hy529 and angiotensin-converting enzyme (ACE) activity. Traditional remedies related to the treatment of hypertension were identified using DongUiBoGam, the most comprehensive source for Korean traditional pharmacopoeia. Herbal remedies were selected by translating the symptom terminology into westernized medicinal terms using the Korean classification of disease in Oriental medicine and Korea Traditional Knowledge Portal. Seven types of herb extracts were selected and screened for in vitro anti-hypertensive activities. Among the extracts, those of Evodiae fructus, Bambusae caulis in Taeniam, Sophorae fructus, Melonis calyx, and Schizonepeta rhizome produced significant increases in NO production, although their ability to inhibit ACE were weaker. Using Fourier transform ion cyclotron resonance mass spectrometry, the major flavonoids exhibiting anti-hypertensive activities were identified: quercetin, apigenin, and luteolin. Our results indicate that these medicinal herbs are potent natural anti-hypertensive agents that can be developed for clinical therapies. In addition, our systematic approach to identifying candidate herbal remedies in the traditional Korean pharmacopoeia can be used to search for additional traditional remedies, such as for hypoglycemia and obesity.

Effective screening for the anti-hypertensive of selected herbs used in the traditional Korean medicines

Kim, Ji Yeon,Kim, Doram,Kwon, Oran The Korean Society for Applied Biological Chemistr 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.4

In this study, Korean medicinal herb extracts were investigated for their in vitro effect on anti-hypertensive activities, including nitric oxide (NO) production in the human umbilical vein endothelial cell line EA.hy529 and angiotensin-converting enzyme (ACE) activity. Traditional remedies related to the treatment of hypertension were identified using DongUiBoGam, the most comprehensive source for Korean traditional pharmacopoeia. Herbal remedies were selected by translating the symptom terminology into westernized medicinal terms using the Korean classification of disease in Oriental medicine and Korea Traditional Knowledge Portal. Seven types of herb extracts were selected and screened for in vitro anti-hypertensive activities. Among the extracts, those of Evodiae fructus, Bambusae caulis in Taeniam, Sophorae fructus, Melonis calyx, and Schizonepeta rhizome produced significant increases in NO production, although their ability to inhibit ACE were weaker. Using Fourier transform ion cyclotron resonance mass spectrometry, the major flavonoids exhibiting anti-hypertensive activities were identified: quercetin, apigenin, and luteolin. Our results indicate that these medicinal herbs are potent natural anti-hypertensive agents that can be developed for clinical therapies. In addition, our systematic approach to identifying candidate herbal remedies in the traditional Korean pharmacopoeia can be used to search for additional traditional remedies, such as for hypoglycemia and obesity.

[철회논문] 말오줌나무(Sambucus sieboldiana var. pendula) 잎 유래 phenol성 물질의 생리활성 효소 저해 및 항균효과

이은호 ( Eun-ho Lee ),홍신협 ( Shin Hyub Hong ),박혜진 ( Hye-jin Park ),김병오 ( Byung-oh Kim ),정희영 ( Hee-young Jung ),강인규 ( In-kyu Kang ),조영제 ( Young-je Cho ) 한국응용생명화학회 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.1

The phenolics of Sambucus sieboldiana var. pendula leaves for functional resources were examined on inhibitory activity against biological enzyme and anti-microbial activity. The amount of phenolic compounds were 11.60±0.18 and 12.43±0.07 mg/g by water and 50% ethanol extraction, respectively. The antioxidative activity of phenolic in extracts was higher than solids. The inhibition activities on angiotensin converting enzyme were 92.08 and 78.33% at 200 μg/mL phenolic concentration in water and ethanol extracts, respectively. The xanthine oxidase inhibitory activity were 100% at 200 μg/mL phenolic in water and ethanol extracts, respectively. These result was higher than 70.37% of allopurinol as positive control at 200 μg/mL. The inhibitory activity against hyaluronidase were each 25.35 and 43.38% in water and ethanol extracts. The water extract from S. sieboldiana var. pendula leaves showed antibacterial activity on the Propione-bacterium acnes, Staphylococcus aureus and Streptococcus mutans in water extract and S. mutans in ethanol extract. This result suggests that phenolic from S. sieboldiana var. pendula leaves are suitable as functional foods with anti-hypertension, anti-gout, anti-inflammation and anti-microorganism activities.

Korea hypertension fact sheet 2018

김현창,Myeong Chan Cho 대한고혈압학회 2019 Clinical Hypertension Vol.25 No.1

Background: The Korea Hypertension Fact Sheet 2018 aims to overview the magnitude and management status of hypertension, and their trends in Korea. Methods: The Hypertension Epidemiology Research Group analyzed the 1998–2016 Korea National Health and Nutrition Examination Survey data and the 2002–2016 Korea National Health Insurance Big Data. Results: The population average of systolic/diastolic blood pressure was 118/77 mmHg among Korean adults (age 30+) in 2016, showing little change in recent 10 years. However, the number of people with hypertension increased steadily, exceeding 11 million. The number of people diagnosed with hypertension increased from 3 million in 2002 to 8.9 million in 2016. The number of people using antihypertensive medication increased from 2.5 million in 2002 to 8.2 million in 2016. However, only 5.7 million people are being treated constantly. Hypertension awareness, treatment, and control rates increased fast until 2007, but showed a plateau thereafter. More than half of the young hypertensive patients (30–49 years) did not know about and treat for their hypertension. Among patients prescribed antihypertensive medications, 45% was elderly people over the age of 65 years, 57% used anti-diabetic or cholesterollowering medications, and 60% were prescribed two or more class of antihypertensive medications simultaneously. Conclusions: In Korea, the level of hypertension management has considerably improved over the last 20 years. In order to achieve further improvement in hypertension management status, we need to find the vulnerable subgroups and develop subgroup-specific intervention strategies. It is also becoming more important to manage hypertensive patients at older age and those with concurrent chronic diseases.

Unusual Case of Posterior Reversible Encephalopathy Syndrome in a Patient with Anti-glomerular Basement Membrane Antibody Glomerulonephritis: A Case Report and Review of the Literature

( Boram Cha ),( Dae Young Kim ),( Hyunil Jang ),( Seun Deuk Hwang ),( Huck Jei Choi ),( Moon-jae Kim ) 대한전해질학회 2017 Electrolytes & Blood Pressure Vol.15 No.1

Posterior reversible encephalopathy syndrome (PRES) is characterized by a clinical and radiological entity with the sudden onset of seizures, headache, altered consciousness, and visual disturbances in patients with the findings of reversible vasogenic subcortical edema without infarction. Hypertension, renal disease, and autoimmune disease are co-morbid conditions of PRES. Nevertheless, there have only been a few case reports of PRES in a patient with anti-glomerular basement membrane antibody glomerulonephritis (anti-GBM GN). This paper presents the possible first Korean case of a 36-year-old woman with the striking features of PRES. She presented with a sudden onset of visual blindness, headache, and seizure. The brain MRI images revealed hyperintense lesions in both the occipital and parietal lobes, which suggested vasogenic edema. Three months before this presentation, she was diagnosed with anti-GBM GN. Since then, she underwent immunosuppression with cyclophosphamide and steroid, and hemodialysis for renal failure with a treatment of anti-GBM GN.

마이크로웨이브 처리에 의한 수수의 항산화, 항당뇨 및 항고혈압 활성 변화

전예솔(Yesol Jeon),이하나(Hana Lee),김현주(Hyun-Joo Kim),허희진(Huijin Heo),홍성화(Seonghwa Hong),김영화(Younghwa Kim),이준수(Junsoo Lee) 한국식품영양과학회 2024 한국식품영양과학회지 Vol.53 No.8

본 연구에서는 마이크로웨이브 처리가 수수의 항산화, 항당뇨 및 항고혈압 활성의 변화에 미치는 효과를 비교하고자 하였다. 그 결과 총 폴리페놀 함량에서 LL 처리 시 123.48 GAE mg/g residue로 가장 높은 함량을 보였으며 이는 처리하지 않은 수수와 비교하여 80.34% 증가한 수치다. 또한 DPPH 라디칼 소거능에서 LL 처리 시 192.03 TE mg/g residue로 가장 높은 활성을 보였으며 총 폴리페놀 함량과 같은 경향을 보였다. α-Glucosidase 저해 활성 측정 시 LL처리에서 38.31%의 억제 활성을 보였으며 이는 Raw 대비 298.48% 증가한 수치였다. HepG2 세포에서 마이크로웨이브 처리 수수의 포도당 흡수율을 측정한 결과 수수 시료를 처리하지 않은 대조군 대비 포도당 흡수율이 24.05~47.39% 수준까지 증가하였다. ACE 저해 활성 측정 시 LL 처리에서 58.75%의 높은 저해율을 보였으며 ML, HL 처리가 각각 55.85%, 54.42%로 뒤를 이었다. H₂O₂로 유도된 산화적 스트레스에 대해 혈관내피세포에서 보호 효과를 측정하였으며 수수의 마이크로웨이브 처리 시 높은 세포 보호 효과를 확인하였다. 따라서 수수에 마이크로웨이브를 장시간 처리시 항산화, 항당뇨 및 항고혈압 활성이 증가하여 열처리 시에도 활성을 증가시킬 수 있는 효과적인 가공 방법이 될 수 있을 것으로 생각된다. 또한 마이크로웨이브 처리 시 생리활성성분의 함량 변화와 생체이용률을 비교하는 연구가 필요할 것으로 사료된다. Sorghum has been studied for its physiological benefits, including its antioxidant, anti-cancer, anti-bacterial, and anti-diabetic properties. It is commonly consumed after undergoing heat treatment. However, this may lead to the loss of some nutritional and functional components. Microwave treatment presents a way to efficiently reduce heat treatment time by quickly raising the food temperature, thereby preserving its nutritional and functional components. This study aimed to investigate the effect of microwave treatment on the antioxidant, anti-diabetic, and anti-hypertensive activity of sorghum, focusing on variations in microwave power and processing time. The results showed that sorghum subjected to a low-power long-term (LL) microwave treatment showed the highest total polyphenol content at 123.48 gallic acid equivalent mg/g residue, which was an 80.34% increase compared to the untreated sample. The radical scavenging activity was the highest with the LL treatment at 192.03 Trolox equivalent mg/g residue, showing the same trend as the total polyphenol content. Moreover, with this treatment, the α-glucosidase inhibitory activity and glucose consumption of microwave-treated sorghum increased by 128.93% to 298.48% and 5.99% to 97.00%, respectively. The angiotensin-converting enzyme inhibitory activity increased by 28.12% to 144.23%, and the protective effects in EA.hy926 cells increased by up to 50.43%. These results indicate that the 100-watt 660-second (LL) microwave treatment is favorable for enhancing the antioxidant, anti-diabetic, and anti-hypertensive activities of sorghum. Additionally, subjecting sorghum to long-term treatment at the same power can further improve its beneficial activity.

택사 열수 및 에탄올 추출물의 항산화활성과 human LDL 산화억제 및 ACE 저해효과에 미치는 영향

양영이,이민자,정현정,이혜숙,김혁,나선택,박선동,박원환,Yang, Young-Yi,Lee, Min-Ja,Jung, Hyun-Jung,Lee, Hye-Sook,Kim, Hyuck,Na, Sun-Taek,Park, Sun-Dong,Park, Won-Hwan 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.4

Objectives : The study was conducted to evaluate antioxidative, anti-atherosclerotic and anti-hypertensive effects of natural remedies. Alisma Rhizome (AR) has been used for a long time in Asia in folk remedies for treatment of hypertension and stroke and has been used in Korean traditional medicine for the treatment of glycosuria, gonorrhea, hypercholesterolemia, hypertension, and jaundice and its diuretic effect. These pharmacological effects of AR might come from antioxidant properties of phytochemicals in these materials. Methods : In this study, the antioxidant activity of extract from AR was studied with in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$ induced human LDL oxidation and on ACE. Results : The AR extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on ACE against all of the reactive species tested, with the water extract showing particularly strong antioxidant activities. Conculsions : The AR extracts have antioxidative, anti-atherosclerotic and anti-hypertensive effects in an in vitro system, which can be used for developing pharmaceutical drugs against oxidative stress and atherosclerosis.

미더덕 껍질, 뽕잎, 양파 추출물을 첨가한 항고혈압 저염간장의 제조 및 특성

신유진(Yu-Jin Shin),이창권(Chang-Kwon Lee),김현진(Hyun-Jin Kim),김현승(Hyoun-Sung Kim),서한극(Han-Geuk Seo),이승철(Seung-Cheol Lee) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.6

고혈압과 같은 혈관성 질환을 개선할 수 있는 항고혈압 저염간장을 개발하기 위하여 미더덕 껍질, 뽕잎, 양파 추출물을 저염간장(염도 12%)에 첨가하였다. 그 결과 단일 소재 추출물을 첨가하였을 때보다 두 가지 이상을 혼합하여 첨가하였을 때 높은 ACE 저해능과 DPPH 라디칼 소거능을 나타내었다. 특히 미더덕 껍질과 양파의 추출물들을 첨가한 저염간장에서 ACE 저해능과 DPPH 라디칼 소거능이 기존의 일반 염도인 15% 간장에 비하여 상대적으로 약 30% 향상되었다. 이상의 결과는 미더덕 껍질, 뽕잎, 양파의 추출물들이 항고 혈압능과 항산화능에 유익한 저염간장을 제조할 수 있는 유익한 소재로 활용될 수 있음을 시사한다. Extracts of Styela clava (Korean name: miduduk) tunic, mulberry, and onion were selected by pre-screening to develop low-salt soy sauce (12% salt content) with anti-hypertensive activity. After choosing the formula for low-salt soy sauce, extracts were added separately or by mixture in combination. In the case of anti-hypertensive activity, low-salt soy sauce containing extracts of miduduk tunic and onion showed 30% increased inhibitory activity towards angiotensin Ⅰ converting enzyme, compared to normal salt soy sauce (15% salt content). Addition of extracts also significantly increased DPPH radical scavenging activities of the soy sauces. These results suggest that natural resources such as miduduk tunic, mulberry, and/or onion might be potential candidates for development of low-salt soy sauce with anti-hypertensive activity.