한국 요석 환자의 Eta-1/Osteopontin 유전자 다형성 분석

김태형,이소연,정우현,오승영,문영태,김경도,김영선,이미경,김혜련,명순철 대한비뇨의학회 2008 Investigative and Clinical Urology Vol.49 No.1

Purpose: Osteopontin(OPN) is one of the major non-collagenous bone matrix proteins produced by osteoblasts and osteolclasts, and it is also involved in the pathogenesis of urolithiasis. Single nucleotide polymorphisms(SNPs), as a tool for searching for the genetic markers of disease, have a large role in investigating the genetic markers of complex human diseases. The aim of this study is to investigate the association with this SNP at position nucleotide 9250(C→T) in the OPN gene and the susceptibility to urolithiasis. We also compared the allele frequency of Koreans with those of Americans and Japanese. Materials and Methods: A total of 161 urolithiasis patients and 104 healthy controls were studied. The SNPs located at position 9520 in the OPN gene were genotyped using restriction fragment length polymorphism(RFLP). The wild-type sequence contains a C while the polymorphism variant is a T(C→T), which results in the appearance of an Alu I restriction site. Results: The gene frequencies of C/C, C/T and T/T at position 9250 on the Eta-1/osteopontin gene in urolithiasis patients were 10.6%, 36.6% and 52.8%, respectively, compared with 6.7%, 27.9% and 65.8%, respectively, in the controls(p>0.05). The allele frequencies of C and T at this position in the urolithiasis patients were 28.9 and 72.1, respectively, whereas those in the controls were 20.7 and 79.3, respectively,(p<0.05). The allele frequencies found in the present study were compared with those coding SNPs described in the USA database; 60 and 39(USA) vs 20.7 and 79.3 (Korea), respectively(p<0.05). Conclusions: Those findings suggest there is no association of with Eta-1/osteopontin genetic polymorphism, but the allele frequencies were significantly associated with urolithiasis patients. We also observed difference of allele frequencies in our controls and in the USA controls and these differences may be caused by a difference in the incidence of urolithiasis patients between the two countries. (Korean J Urol 2008;49:55-59) Purpose: Osteopontin(OPN) is one of the major non-collagenous bone matrix proteins produced by osteoblasts and osteolclasts, and it is also involved in the pathogenesis of urolithiasis. Single nucleotide polymorphisms(SNPs), as a tool for searching for the genetic markers of disease, have a large role in investigating the genetic markers of complex human diseases. The aim of this study is to investigate the association with this SNP at position nucleotide 9250(C→T) in the OPN gene and the susceptibility to urolithiasis. We also compared the allele frequency of Koreans with those of Americans and Japanese. Materials and Methods: A total of 161 urolithiasis patients and 104 healthy controls were studied. The SNPs located at position 9520 in the OPN gene were genotyped using restriction fragment length polymorphism(RFLP). The wild-type sequence contains a C while the polymorphism variant is a T(C→T), which results in the appearance of an Alu I restriction site. Results: The gene frequencies of C/C, C/T and T/T at position 9250 on the Eta-1/osteopontin gene in urolithiasis patients were 10.6%, 36.6% and 52.8%, respectively, compared with 6.7%, 27.9% and 65.8%, respectively, in the controls(p>0.05). The allele frequencies of C and T at this position in the urolithiasis patients were 28.9 and 72.1, respectively, whereas those in the controls were 20.7 and 79.3, respectively,(p<0.05). The allele frequencies found in the present study were compared with those coding SNPs described in the USA database; 60 and 39(USA) vs 20.7 and 79.3 (Korea), respectively(p<0.05). Conclusions: Those findings suggest there is no association of with Eta-1/osteopontin genetic polymorphism, but the allele frequencies were significantly associated with urolithiasis patients. We also observed difference of allele frequencies in our controls and in the USA controls and these differences may be caused by a difference in the incidence of urolithiasis patients between the two countries. (Korean J Urol 2008;49:55-59)

요로 결석 환자에서 급성 신우신염의 예측인자로 hs-C-reactive protein의 유용성

박진욱,진상찬,김성진,최우익 대한응급의학회 2014 대한응급의학회지 Vol.25 No.4

Purpose: The aim of this study was to determine early predictivevalue of acute pyelonephritis and urosepsis inpatients with urolithiasis in the emergency department. Methods: We retrospectively reviewed medical records ofpatients who visited the emergency department and werediagnosed with urolithiasis by computed tomography forthree years. Patients with urolithiasis were grouped accordingto the presence of computed tomography (CT) findingswith acute pyelonephritis. In baseline characteristics, laboratoryand CT findings of the two groups were compared. Group 1 was defined as urolithiasis without acutepyelonephritis and group 2 was defined as urolithiasis withacute pyelonephritis. In addition, we compared the sepsisversus non-sepsis and percutaneous nephrostomy (PCN)versus non-PCN group in Group 2 for analysis of the hs-CRP level of each group. Results: The total number of urolithiasis patients was 744. Among the patients, 84 (11.3%) had urolithiasis with acutepyelonephritis in CT findings. Age, sex, history of diabetes,history of urolithiasis, size of stone, duration of symptom,body temperature, blood pressure, heart rate, respiratoryrate, leukocyte count, existence of pyuria, and hs-CRP differedsignificantly between the two groups, respectively. Inmultivariate logistic regression analysis, age, history ofurolithiasis, existence of pyuria, and hs-CRP were shown tobe independent predictors affecting acute pyelonephritis inpatients with urolithiasis. The area under the receiving operatorcharacteristics (ROC) curve for CRP was 0.820 (95%CI, 0.754-0.886) and leukocyte count was 0.631 [95% confidenceinterval (CI), 0.542-0.721]. Sepsis and PCN groupsshowed significantly higher hs-CRP level than non-sepsisand non-PCN groups. Conclusion: There were some independent predictive valuesof urolithiasis with acute pyelonephritis. It can be usefulin early detection of acute pyelonephritis or sepsis, and itcan be helpful in making treatment plans for patients ofurolithiasis.

Is the Serum Uric Acid Level Independently Associated with Incidental Urolithiasis?

( Doo-ho Lim ),( Min-ho Kim ),( Seokchan Hong ),( Yong-gil Kim ),( Chang-keun Lee ),( Seung Won Choi ),( Bin Yoo ),( Ji Seon Oh ) 대한류마티스학회 2018 대한류마티스학회지 Vol.25 No.2

Objective. Urolithiasis is one of the manifestations of gout and the risk is higher in gouty patients. On the other hand, an independent association between the urinary stone and serum uric acid (UA) level has not been established. This study examined whether the risk of urolithiasis increases with increasing serum UA level. Methods. Among the people who visited a tertiary hospital from 2010 to 2013, 13,964 individuals who underwent both ultrasonography and a laboratory test were recruited in the study. The risk of urolithiasis on ultrasonography was analyzed in association with the serum UA level by multiple logistic regression analysis with an adjustment for age, sex, body mass index, estimated glomerular filtration rate, and known underlying diseases, including diabetes mellitus and hypertension. Results. Among the 6,743 men (48.3%) and 7,221 women (51.7%), the age was 51.3±13.5 and the serum UA level was 4.5±2.1 mg/dL. Hyperuricemia (>7 mg/dL) was observed in 1,381 cases (9.9%). Urolithiasis was detected by ultrasonography in 608 cases (4.4%). The detection rates of urolithiasis in individuals with hyperuricemia and normouricemia were 5.9% and 4.1%, respectively (p=0.001). Multiple logistic regression analysis showed that individuals with hyperuricemia had a significantly higher risk of urolithiasis (adjusted odds ratio [OR]=1.54; 95% confidence interval [CI], 1.20∼1.96; p=0.001). A comparison of the highest with the lowest quartile of serum UA revealed a multivariable- adjusted OR of 3.17 (95% CI, 1.98∼5.11) for men and 1.79 (1.08∼2.93) for women. Conclusion. These results suggest that individuals with a higher serum UA level have a higher risk of subclinical and clinical urolithiasis. (J Rheum Dis 2018;25:116-121)

Eleven-year Cumulative Incidence and Estimated Lifetime Prevalence of Urolithiasis in Korea: a National Health Insurance Service-National Sample Cohort Based Study

태범식,Ulanbek Balpukov,조성용,정창욱 대한의학회 2018 Journal of Korean medical science Vol.33 No.2

Background: The purpose of this study was estimation of the cumulative incidence and lifetime prevalence of urolithiasis in Korea. Methods: We used a National Health Insurance Service (NHIS) sample cohort dataset that included approximately 1 million individuals from Korea. Data from January 2002 to December 2013 were collected. We calculated the annual prevalence, recurrence rate, and estimate lifetime prevalence of urolithiasis. Multivariate logistic regression analysis was used to identify risk factors associated with urolithiasis. Results: There were 57,921 diagnosed urolithiasis cases in the NHIS database over the 11 years studied. The annual incidence of urolithiasis increased every year (Poisson regression; hazard ratio, 1.025; P < 0.001). Of the patients with urolithiasis, 21.3% experienced disease recurrence within 5 years. The 11-year cumulative incidence was 5.71%, and the incidence in men was higher than that seen in women (7.07% vs. 4.34%, respectively). The 11-year cumulative incidence in the 60- to 69-year-old group (9.08%) was higher than that seen in any other age group. The overall standardized lifetime prevalence rate was estimated to be 11.5%: 12.9% in men and 9.8% in women. Meanwhile, age (> 60 years), income level, diabetes, body mass index, hypertension, and cancer history were identified as contributing factors to urolithiasis. Conclusion: This study demonstrates that the annual incidence of urolithiasis in Korea is increasing. The overall standardized lifetime prevalence rate was higher than that reported in a previous report. This study is significant in that it is the first retrospective cohort study to estimate the lifetime prevalence of urolithiasis using a large national retrospective cohort.

Epidemiology of Urolithiasis with Sex and Working Status Stratification Based on the National Representative Cohort in Republic of Korea

Heo Jun,Son Jeongmin,Lee Wanhyung 한국산업안전보건공단 산업안전보건연구원 2022 Safety and health at work Vol.13 No.4

Background This study aimed to estimate the annual prevalence and incidence of urolithiasis stratified by work status based on a large nationwide sample. Methods This study used data from the National Health Insurance Service-National Sample Cohort from 2002 to 2015. The prevalence and incidence of urolithiasis were estimated based on work status and gender stratification. The risk of urolithiasis among workers was calculated using age-standardized incidence ratio with stratification of work type. Results The prevalence of urolithiasis was significantly higher in workers than in non-workers, especially men, during the follow-up period. The total estimated number of urolithiasis cases was 41,086 and the overall incidence of urolithiasis was 0.3%. The age-standardized incidence ratio of urolithiasis was significantly higher among the total workers (1.14; 95% confidence interval, 1.13–1.16), self-employed workers (1.08; 95% confidence interval, 1.06–1.11), and paid workers (1.19; 95% confidence interval, 1.17–1.21) than among the non-working population. Conclusions Workers, especially paid workers and men, were vulnerable to urolithiasis. Further studies are required to investigate the effects of working conditions on urolithiasis.

Interleukin-1β, Calcium-Sensing Receptor, and Urokinase Gene Polymorphisms in Korean Patients with Urolithiasis

Kim, Joon-Young,Kim, Young-Sun,Jang, In-Ho,Jung, Jae-Dong,Kim, Tae-Hyoung,Kim, Hye-Ryun 대한비뇨기과학회 2011 Investigative and Clinical Urology Vol.52 No.5

<P><B>Purpose</B></P><P>There are various causes of ureter calculi, and genetic factors are known to play a role. Interleukin-1β (IL-1β) and calcium-sensing receptor (CaSR) genes are related to hypercalciuria, and urokinase is related to the formation of calcium oxalate stones. This study investigated polymorphisms in IL-1β, CaSR, and urokinase in patients with urolithiasis and healthy controls.</P><P><B>Materials and Methods</B></P><P>Urolithiasis patients treated at Chung-Ang University Hospital were enrolled from January 2007 to December 2008. The control group of volunteers displayed normal urinalysis findings in the health screening, no stones identified by ultrasonography, and no history of urolithiasis. DNA extracted from peripheral blood was analyzed by the polymerase chain reaction. Patients were genetically screened for mutations in IL-1β (484 urolithiasis patients, 208 controls), CaSR (433 urolithiasis patients, 197 controls), and urokinase (370 urolithiasis patients, 167 controls). Stone metabolic study was done to see the differences between the metabolic factors and to discern normal genes from polymorphic genes.</P><P><B>Results</B></P><P>According to the genotype frequency and allele frequency analysis, there were no statistically significant differences between IL-1β, CaSR, and urokinase genes. Also, the analysis between genotypes and metabolic factors did not show statistically significant differences between the three genes.</P><P><B>Conclusions</B></P><P>In Korean urolithiasis patients, IL-1β, CaSR, and urokinase gene polymorphisms do not differ from those of healthy individuals. A larger-scale study is needed to confirm the need for other genetic markers of urolithiasis.</P>

요로결석의 위험인자

오경진 대한의사협회 2020 대한의사협회지 Vol.63 No.11

Urolithiasis is one of the most common urologic diseases in Korea. The annual incidence of urolithiasis in Korea has increased over time. Understanding the epidemiology and risk factors for urolithiasis is essential for patient care and prevention. The purpose of this review was to summarize the recent literature regarding the risk factors for urolithiasis. The available Korean-population-based trials were enrolled in this study. The peak incidence age was the ffth to sixth decades in Korea. There has been a persistent male predominance in the prevalence and incidence of stone disease. The gender gap has continuously narrowed over the world. Climate and seasonal variations, such as temperature and humidity, have a profound effect on the development of urolithiasis. Various dietary factors, including calcium, sodium, animal protein, and oxalate, play an important role in the development of urolithiasis. Dehydration is a wellknown risk factor for stone disease. Many metabolic diseases can affect urolithiasis, including obesity, hypertension, diabetes, gout, and metabolic syndrome. The fast-moving super-aged society and global warming can increase stone disease burden in the near future. To prevent and reduce stone diseases, clinicians should understand the risk factors affecting stone formation.

요석 발생의 위험요소로서 비만과 고지혈증의 의의

김형주,전윤수,이남규 大韓泌尿器科學會 2000 Korean Journal of Urology Vol.41 No.6

Dietary risk factors for urolithiasis in Korea: A case-control pilot study

류호영,이유경,박주현,손환철,조성용 대한비뇨의학회 2018 Investigative and Clinical Urology Vol.59 No.3

Purpose: Dietary factors are one of the main causes of urolithiasis. However, little research has evaluated dietary factors related to urolithiasis in Korea. We investigated the various dietary risk factors for urinary stone formation in Korean people. Materials and Methods: We conducted a prospective case-control pilot study. A total of 27 patients newly diagnosed with urolithiasis and 20 applicants without urolithiasis were designated as the patients and the control group, respectively. A face-to-face survey was carried out using a food-frequency questionnaire. After adjustment for physical activity level and total energy intake, multivariate logistic regression models were applied to search for risk factors for urolithiasis. Results: There were no significant differences between the two groups in gender, age, body mass index, family history, or total energy intake. The physical activity level of the control group was significantly higher than that of the patients (p=0.012). The results of the multivariate logistic regression model demonstrated that intake of carbohydrate (odds ratio [OR], 1.055; 95% confidence interval [CI], 1.012–1.099), protein (OR, 1.101; 95% CI, 1.001–1.211), and cereals (OR, 1.012; 95% CI, 1.002–1.023) could increase the risk for urolithiasis. Conclusions: A higher intake of carbohydrate, protein, and cereal may increase the risk of urinary stone formation among Korean people.

Dynamic thiol/disulfide homeostasis as a novel indicator of oxidative stress in patients with urolithiasis

Mehmet Giray Sonmez,Betul Kozanhan,Çigdem Damla Deniz,Mehmet Sinan Iyisoy,Muzaffer Tansel Kilinc,Gokhan Ecer,Ahmet Ozturk,Salim Neselioglu,Ozcan Erel 대한비뇨의학회 2019 Investigative and Clinical Urology Vol.60 No.4

Purpose: A dynamic thiol/disulfide balance is pivotal in organizing anti-oxidant defense, detoxification, apoptosis, and enzyme activities, as well as transcription and cellular signal-transfer mechanisms. The connection between urolithiasis and oxidant/antioxidant status, which can be assessed through thiol-disulfide homeostasis (TDH), has not yet been examined. In this study, we evaluated the effects of TDH on the formation, size, and location of stones by examining the associations between TDH parameters and urolithiasis. Materials and Methods: Patients with urolithiasis and healthy controls were recruited. The patients were divided into subgroups in terms of stone size (>15 mm or ≤15 mm) and stone location (nephrolithiasis or ureterolithiasis). TDH parameters were measured using a novel automatic and spectrophotometric method and compared statistically. Results: TDH parameters were different between the urolithiasis and control groups. TDH tended towards the disulfide side in the urolithiasis group. Stone size increased an average 0.14 mm with a 1 µmol/L increase in disulfide level and decreased an average 0.058 mm with a 1 µmol/L increase in native thiol level. Disulfide and native thiol levels were found to be different across patients with stone size >15 mm, ≤15 mm, and controls (p<0.001 and p<0.001, respectively). However, the nephrolithiasis and ureterolithiasis groups were similar in respect of TDH parameters. Conclusions: In this study, it was found that patients with urolithiasis displayed oxidative stress characterized by a TDH tendency towards the disulfide side, and an inadequate antioxidant response identified by a lower level of native thiol as compared with healthy controls.