UV-Vis spectrophotometry법을 이용한 다양한 유지류로부터 헴프씨드 오일의 진위 판별법

이윤진(Yun-Jin Lee),강덕경(Deok-Gyeong Kang),김영민(Young-Min Kim),손호용(Ho-Yong Sohn) 한국생명과학회 2022 생명과학회지 Vol.32 No.5

대마씨의 외종피를 탈각하여 Tetrahydrocanabinol이 제거된 헴프씨드는 건강식품으로 각광받고 있으며, 헴프씨드 오일은 α-linolenic acid 및 γ-linolenic acid 등의 다가 불포화 지방산 및 지용성 비타민 등이 풍부하여, 인지 기능 및 운동기능 개선, 콜레스테롤 감소, 혈관 염증 완화, 혈전 생성 억제, 신경세포 손상 방지 등의 효능이 알려지면서 수요가 급증하고 있다. 현재 헴프씨드 오일은 여타의 식물성 유지(콩기름, 옥수수유, 올리브유, 카놀라유, 아미씨유)보다 45~140배 이상 고가로 판매되고 있다. 본 연구에서는 FTIR, FTIR-Raman, ATR-FTIR-MIR spectroscopy와 같은 고가의 장비와 전문인력에 의한 분석 없이도 헴프씨드 오일의 진위여부를 판별할 수 있는 간편한 UV-Vis spectrophotometry법을 개발하고자 하였으며, 다양한 오일의 245, 305, 및 415 nm 흡광도를 측정하고, 245/415 nm 및 305/415 nm에서의 흡광도의 비를 계산하여 12.9 및 9.6이 나타나는 경우 헴프씨드 오일임을 확인하였다. 콩기름, 옥수수유, 카놀라유 및 아미씨유의 경우에는 각각 35.4~61.8 및 29.7~50.8을 나타내어 헴프씨드 오일과 쉽게 구분 가능하였다. 상기의 방법은 헴프씨드 오일에 여타의 식물성 유지류를 혼합하는 경우에도 혼합 여부 판별에 이용 가능하였다. Hempseed, a dehulled Cannabis fructus, has high nutraceutical potential. It has plenty of essential amino acids, vitamins, and essential polyunsaturated fatty acids, including α- and γ-linolenic acid. Increased exercise capacity, cognitive function, and ameliorative effects against hypercholesterolemia, neuro-inflammation, thrombus formation, and learning and memory impairment were reported in hempseed oil-administered models. Therefore, the market prices of hempseed oil are 45~140-fold higher than the other plant-derived oils, such as soy, corn, olive, canola, or linseed oil. In this study, instead of FTIR (Fourier Transform Infrared Spectroscopy) or FTIR-Raman spectroscopy, a simple UV-Vis spectrophotometry method was developed to authenticate the hempseed oil. Measurements of absorbance at 245, 305, and 415 nm of oils and calculations of 245/415 and 315/415 nm provided that the ratios of 245/415 and 315/415 nm of authentic hempseed oils were 12.9 and 9.6, respectively. The 245/415 and 315/415 nm of soy oil, corn oil, canola oil, and linseed oil were 35.4~61.8 and 29.7~50.8, respectively. This simple UV-Vis spectrophotometry method could also be applied to differentiate hempseed oil from blended oil products in markets.

해수 중 엽록소 a 측정방법에 대한 실험실 간 비교연구

문초롱,강동진,박미옥,노재훈,유신재,문정언,신경훈,김윤숙,최중기,서영상,Moon, Cho-Rong,Kang, Dong-Jin,Park, Mi-Ok,Noh, Jae Hoon,Yoo, In-Jae,Moon, Jeong-Eon,Shin, Kyung-Hoon,Kim, Yun Sook,Choi, Joong-Ki,Suh, Young Sang 한국해양학회 2014 바다 Vol.19 No.1

해양식물플랑크톤 생물량 및 일차생산력을 추정하기 위한 자료로서 이용되는 해수 중 엽록소 a의 농도는 고성능 액체크로마토그래피(High Performance Liquid Chromatography, HPLC), 형광법(Fluorometry), 분광광도법(Spectrophotometry)등의 분석방법으로 측정된다. 본 연구에서는 여러 실험실에서 이러한 방법들로 분석된 엽록소 a 자료를 통용하기 위한 기초자료로서 활용하고자 우리나라 주변 해역의 현장 시료를 이용한 상호비교실험을 실시하여 엽록소 a 분석 실험실 및 분석 방법간의 차이를 비교하였다. 상호비교실험은 엽록소 a 표준색소(R0) 및 동중국해(R1)와 동해해역(R2)에서 채수된 해수 현장 시료를 이용하여 총 3회 시행되었다. 참가실험실은 각각 HPLC(6개 실험실), 형광법(4개 실험실), 분광광도법(3개 실험실)으로 시료를 분석하였다. 표준색소와 현장시료의 측정결과에서 얻은 실험실 내 정밀도는 변동계수로 평가되었으며, 표준색소(R0)에서 9% 미만, 현장시료에서는 R1: 0.8~20%(평균 6.1%), R2: 4~21%(평균 13.2%)의 정밀도를 보였다. 전체 현장시료의 모집단에서 z-test를 이용하여 이상치를 제거한 측정결과들의 중앙값을 기준 값으로 평가한 HPLC, 형광법, 분광광도법 간의 차이는 20%이내였다. 이러한 차이는 현장시료의 균질성 및 실험실 내의 정밀도를 고려한 차이(R1: 8%, R2: 15%)와 유사한 값을 보였다. 비교결과로 미루어 볼 때, 측정값들간의 차이는 분석방법의 차이보다는 실험실 간 차이가 더 크다고 볼 수 있다. 따라서 각 실험실에서 생산된 자료는 분석 방법이 다르더라도 약 20% 이내에서는 동일한 결과를 생산한다고 볼 수 있으므로 타 실험실에서 서로 다른 방법으로 분석된 엽록소 a의 자료를 활용하는 경우에는 약 20%의 차이에 대한 고려가 필요하다. Chlorophyll a in seawater which is an indicator of phytoplankton biomass and primary production is determined by High Performance Liquid Chromatography (HPLC), Fluorometry and Spectrophotometry. In this study, various methods for chlorophyll a determination in seawater are compared using in situ seawater samples from Korean seas. Three inter-laboratory comparison campaigns were carried out using chlorophyll a standard samples (R0) and in situ seawater samples, collected from the East China Sea (R1) and the East Sea (R2). 6 laboratories by HPLC methods, 4 laboratories by fluorometry, and 3 laboratories by spectrophotometry participated. Precisions, defined as the coefficient of variation (CV) were within 9% in standard samples, 0.8~20% (average: 6.1%) in R1, 4~21% (average: 13.2%) in R2. Discrepancy in three methods was approximately 20% within the range of the sample homogeneity intended the laboratory precision (R1: 8%, R2: 15%). The discrepancy in laboratories was greater than the discrepancy in methods. The chlorophyll a concentrations can be produced within 20% discrepancy in spite of using different methods. It is recommended to consider this 20% discrepancy when using the chlorophyll a data produced by different laboratories and different methods.

비파괴 자외-가시 및 형광 분광 분석법을 이용한 황색계 전통염료 분석

유혜선,윤은영,김유란 국립중앙박물관 2010 박물관보존과학 Vol.11 No.-

본고는 우리나라 황색계 염재인 울금, 황벽, 황련, 치자, 괴화로 염색한 직물편에 대한 비파괴 자외-가시광 분광 분석 및 3차원 형광 분광 분석 내용이다. 직물이나 매염제 종류에 따라서 각 염재의 분석결과에 영향을 주는지 여부를 확인하기 위하여 직물 2종류(견과 면)와 매염방법 3가지(무매염, 백반, 철)로 염색한 직물시편을 제작하였다. 이 염색 직물편에 대한 자외-가시 분광반사 스펙트럼 측정 결과, 울금, 황벽, 황련으로 염색한 경우 직물의 종류에 관계없이 무매염과 백반매염한 시편의 결과가 유사한 반면 철매염한 시편에서는 차이를 보였다. 또한 치자로 염색한 경우 직물의 종류에 따라 다른 결과를 보였으나 매염제에는 영향을 받지않았다. 반면에 괴화로 염색한 경우 직물의 종류에 관계없이 매염제에 따른 차이를 보였다. 3차원 형광 스펙트럼 측정 결과 울금, 황벽, 황련으로 염색한 직물편은 직물과 매염제에 관계없이 고유한 형광 스펙트럼으로 나타났으나 치자의 경우 직물의 종류에 따라 차이를 보인 반면 괴화의 경우는 직물의 종류에는 관계가 없었으나 매염제에 따른 차이를 보였다. This is the analysis of material dyed with Korean yellow dyes such as tumeric, amur cork tree, goldthread, gardenia, and the flowers of sophora japonica using nondestructive ultraviolet-visible and fluorescence spectrophotometry. In order to find out whether type of fabric or mordant influences analysis results, test fabrics were made using two types of fabric(silk and cotton) and dyed using three different mordants(no mordant, alum, iron). After analysis with UV-Vis reflectance spectrum on the dyed fabric, when the fabric was dyed with tumeric, amur cork tree and goldthread, the results were similar with no mordant and alum mordant, whereas there was a difference with an iron mordant. Also when the fabric was dyed using gardenia, different fabrics brought different results but there was no difference in results with mordants. On the other hand, when the fabric was dyed using the flowers of sophora japonica, there was no difference with fabrics but with mordants. After analysis with 3D-fluorescence spectrum, fabrics dyed with tumeric, amur cork tree and goldthread showed their own fluorescent spectrum with no regard to fabric and mordant; but with gardenia, there were differences with different fabrics whereas with the flowers of sophora japonica, there were differences with mordants.

Benzophenone에 Oxine착물로 흡착 농축시킨 후 니켈(Ⅱ)의 정량

최희선 수원대학교 기능성생명소재연구소 2003 자연과학연구논문집 Vol.2 No.1

A technique for the determination of nickel after the preconcentration by adsorbing its oxine complex onto benzophenone was developed. Several experimental conditions such as the pH of the solution, the amount of oxine and benzophenone and stirring time were optimized. Trace nickel in 100mL water sample was chelated with 1.5mL of 0.02M oxine at pH 6.0. After 0.15g benzophenone was added, the solution was stirred for 30minutes. The benzophenone containing Ni(Ⅱ)-oxine complex was filtered and dissolved in ethyl alcohol. Nickel was determined by flame atomic absorption spectrophotometry. The interfering effects of diverse metal ions were investigated and eliminated. This method could be applied to natural water samples and the recovery of about 95% was obtained in the real samples.

비이온성 미셀내에서 킬레이트제와 그의 금속 착물의 용해도와 안정도

최희선 수원대학교 기초과학연구소 1995 基礎科學論文集 Vol.4 No.-

It was appeared that 1-nitroso-2-naphthol slightly dissolved in aqueous solution, but dissolved in 5% Tween 80 solution well as 770mg/100mL of its solubility. As the absorption spectra of 1-nitroso-2naphthol complexes of Cu(Ⅱ), Co(Ⅱ), Ni(Ⅱ), and Fe(Ⅲ) were able to obtain in 5% Tween 80 solution, it was proved that its metal complexes could also be dissolved in nonionic micelle. And 1-nitroso-2naphthol and its Cu(Ⅱ), Co(Ⅱ), Ni(Ⅱ), and Fe(Ⅲ) complexes in 5% Tween 80 solution were stable for at least 100 minutes. From the results of this experiment, it is expected that its Cu(Ⅱ), Co(Ⅱ), Ni(Ⅱ), and Fe(Ⅲ) complexes in nonionic surfactant Tween 80 solution can be determined by UV-Vis spectrophotometry.

ASSESSMENT OF PORCINE FAT QUALITY BY FIBER-OPTIC SPECTROPHOTOMETRY

Irie, M.,Swatland, H.J. Asian Australasian Association of Animal Productio 1992 Animal Bioscience Vol.5 No.4

Research was undertaken to determine if relectance (interactance) measured with a portable fiver optic probe (Colormet) can be used to assess porcine fat quality. Soft fat generally had lower interactance than hard fat, p<0.05 from 400 to 700 nm, although interactance spectra of hard and soft fat were similar in shape. At $4^{\circ}C$, interactance from 450 nm to 700 nm at the inner layer of backfat was correlated (p<0.01) with subjective soft fat score (r = 0.06 to 0.70), and with the refractive index (r = -0.62 to -0.65) and melting point (r = 0.59 to 0.60) of heat-extracted lipid. Colormet interactance $L^*$ at $4^{\circ}C$ was correlated (p<0.01) with soft fat score (r = 0.72), refractive index (r = -0.66) and melting point (r = 0.61). Interactance decreased as the temperature of the fat was increased from 22 to $44^{\circ}C$ (p<0.01). Soft fat had lower interactance than fats that were slightly soft, slightly hard and hard at 4, 22 and $40^{\circ}C$, although softness and temperature may interact to affect interactance. These results indicate that soft porcine fat may be detected easily by fiber-optic spectrophotometry.

성숙기 사과 중의 페놀계 물질 변화

황혜정 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.4

성숙기 사과의 페놀계 물질 변화를 살펴보고자 부사품종 사과의 methanol 추출물을 Sep-Pak으로 전처리하여 HPLC로 측정한 결과 4종의 phenolic acid와 (+)-catechin를 분리할 수 있었다. 또한 본 연구에서 측정한 chlorogenic acid와 (±)-catechin을 비롯한 페놀계 물질들의 함량은 성숙과정 중 전체적으로 감소하였으나 그 변화의 폭은 매우 완만하였다. 즉 발육과정 중 페놀계 물질이 감소하다가 어느 시기부터는 일정한 함량치를 나타냈다. 또한 총페놀함량은 Folin-Dennis법으로 측정하였는데 성숙초기에 약간 감소하였다가 그 후 함량차가 거의 없는 것으로 나타났다. The changes of phenolics in Korean apple(Fuji) during maturation were analyzed by HPLC and spectrophotometry. The phenolics were separated through C_18 Sep-Pak cartridge in series. Chlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, and (±) catethin were indentified by the direct comparison with authentics on HPLC. The total amounts of phenolics were determined by Folin-Dennis's method. The amounts ranged from 70.19㎎% to 97.57㎎% of wet basis. The concentration of phenolics in apple decreased during the early stage of development and then remained relatively constant during maturation.

Matrix Modification for Graphite Furnace Atomic Absorption Spectrophotometric Determination of Volatile Elements(III) Trace Bismuth

Kim, Young-Sang,Choi, Jong-Moon,Kim, Young-Man 한국분석과학회 1995 분석과학 Vol.8 No.4

A matrix modification was studied for the determination of trace bismuth in water samples by graphite furnace atomic absorption spectrophotometry. The type and quantity of modifiers as well as the use of auxiliary modifiers were investigated to realize the efficient modification. Palladium was chosen as a single modifier. By the addition of palladium($5{\mu}g/mL$) to 100 ng/mL bismuth solution, the temperatures could be raised from $500^{\circ}C$ to $1,300^{\circ}C$ for the charring and from $2,000^{\circ}C$ to $2,200^{\circ}C$ for the atomization as well as the sensitivity and reproducibility were improved. The absorbance of bismuth was maximum and not changed in the range of Pd $3-25{\mu}g/mL$. And several materials were examined as an auxiliary modifier. The mixed solution of $1{\mu}g/mL$ palladium and $200{\mu}g/mL$ nickel have raised the temperatures as with $5{\mu}g/mL$ palladium only. The maximum absorbance of bismuth was shown in the nickel concentration range of $100-300{\mu}g/mL$ in $1{\mu}g/mL$ palladium modified system. With such optimum conditions, the trace amount of bismuth in several water samples could be determined by a calibration curve method, and good recoveries were also obtained.

Spectrophotometric study of the uranyl monobenzoate complex at moderate ionic strength

Choi, Seonggyu,Yun, Jong-Il Pergamon Press 2019 Polyhedron Vol.161 No.-

<P><B>Abstract</B></P> <P>The protonation of benzoate and the complexation of uranyl monobenzoate in NaClO<SUB>4</SUB> solutions ranging from 0.1 to 0.5 M were quantitatively investigated by means of ultraviolet–visible (UV–Vis) spectrophotometry. The spectrophotometric study revealed that the uranyl monobenzoate species has a significantly enhanced absorption than that of free uranyl, especially in the UV region. The non-linear regression analysis of the UV–Vis absorption data was the first to calculate the protonation constant of benzoate and the stability constant of uranyl monobenzoate at moderate ionic strength. Based on the SIT (specific ion interaction theory), the protonation constants were extrapolated to the zero ionic strength as log β P ° = 4.21 ± 0.01, and the ion interaction coefficient of the benzoate anion with the sodium cation was determined as ε ( <SUP> Ben - </SUP> , <SUP> Na + </SUP> ) = 0.17 ± 0.05 kg·mol<SUP>−1</SUP>. In the same manner, the stability constant of uranyl monobenzoate at zero ionic strength and the ion interaction coefficient of the uranyl monobenzoate cation with perchlorate anion were determined to be log β U ° = 2.91 ± 0.01 and ε ( <SUP> <SUB> UO 2 </SUB> B e n + </SUP> , <SUP> <SUB> ClO 4 </SUB> - </SUP> ) = 0.22 ± 0.07 kg·mol<SUP>−1</SUP>, respectively.</P> <P><B>Graphical abstract</B></P> <P>The stability constants and spectroscopic properties of the uranyl monobenzoate complex in 0.1–0.5 M NaClO<SUB>4</SUB> medium were investigated by UV–Vis spectrophotometry. The ion interaction coefficient of the uranyl monobenzoate cation with perchlorate anion was determined based on the SIT for the first time.</P> <P>[DISPLAY OMISSION]</P>

Highly Selective and Simple Zero and First Order Derivative Spectrophotometric Determination of Palladium by Using α-Benzilmonoxime in Triton X-100 Micellar Solution

Eskandari, Habibollah,Karkaragh, Gholamhassan Imanzadeh Korean Chemical Society 2003 Bulletin of the Korean Chemical Society Vol.24 No.12

The reaction of ${\alpha}$-Benzilmonoxime with palladium(II) produces a green complex in triton X-100 micellar media. Palladium has been determined using zero and first derivative spectrophotometric methods. The absorbances of Pd(II)- ${\alpha}$--benzilmonoxime complex at 441.8 and 677.0 nm in 0.10 M perchloric acid solution were monitored and linear working ranges of 0.3-12.0 and 0.7-20 ${\mu}$g mL$^{-1}$ with detection limits of 0.07 and 0.10 ${\mu}$g $mL^-1$ were obtained, respectively. Also, recoveries in the range of 92.8 to 100.1% and relative standard deviations in the range of 0.4 to 7.1% were obtained. First derivative spectrophotometry has also been applied for palladium determination under the optimum condition. The linear dynamic range of 0.2-24.0 ${\mu}$g $mL^{-1}$ palladium with relative standard deviations of 0.6-6.9% and recoveries in the range of 94.9-102.5% has been obtained by first derivative spectrophotometry. The method shows high selectivity because of the high concentration of acid used, which prevents formation of complexes of ${\alpha}$--benzilmonoxime with the other cations. The palladium complex formed was stable at least one day. The method was successfully applied to the determination of palladium in some synthetic palladium alloys and palladium-charcoal powder.