Effect of sawdust particle size on larval growth and oviposition preference of Protaetia brevitarsis

Younjeong Mo,Hae-chul Park,In-gyun Park,Seonghyun Kim 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10

In general, the larvae of Protaetia brevitarsis is raised on fermented oakwood sawdust. Many attempts have been to changes components sawdust, such as using other tree instead of oakwood or adding another material. Like this some study exists, about 'component' of sawdust, however little is known of relation between larval growth and quality of sawdust. The phenomenon-big particle size sawdust flows up on excrement from the larvae-is occurring in used sawdust for raising the larvae. This study was conducted to observe the larvae and adult of Protaetia brevitarsis act according to each sawdust particle size. We conducted choice experiments with Protaetia brevitarsis, which were offered sawdust differing in particle size. Females preferred to lay their eggs on the fine particle sawdust. Also in the case of larvae, the more fine sawdust particle has the more high level of preference, and so is hatchability. Especially larvae tend to be slanted toward finer sawdust. Growth rate of the larvae was shown the highest level in the finest sawdust just as others do. These results show that fine sawdust particle size is advantage for larval development and oviposition.

Characteristics of Sawdust and Cocopeat Beddings, and Their Usefulness According to the Fan and Pen Location for Rearing Hanwoo Cattle

Ahn, Gyu Chul,Jang, Sun Sik,Lee, Kang Yeon,Kwak, Wan Sup,Oh, Young Kyun,Park, Keun Kyu Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.3

This study was designed to examine the characteristics of sawdust and cocopeat bedding materials, including physicochemical properties (Exp. I) and on-farm trial (Exp. II). In Exp. I, the proportion of particle size was in the order of sawdust>cocopeat India>cocopeat Vietnam (p<0.05), and cocopeat contained higher proportion of small particles ($250{\mu}m$+below $250{\mu}m$) than sawdust, causing a dust production problem. Bulk density was cocopeat India>cocopeat Vietnam>sawdust (p<0.05), thus cocopeat treatments showed 4.4 times higher bedding cost than sawdust. The water absorption rates were 702.0% in cocopeat India, 678.3% in cocopeat Vietnam, and 444.0% in sawdust, showing cocopeat had approximately 1.5 times higher water absorption rate than sawdust. Moisture evaporation rates after 12 h of air blowing (2.00 m/s) were higher (p<0.05) in cocopeat Vietnam (80.4%) than sawdust (71.2%) and cocopeat India (72.8%). In vitro ammonia emissions were higher (p<0.05) in sawdust ($2.71mg/m^2/h$) than cocopeat India ($1.59mg/m^2/h$) and Vietnam ($1.22mg/m^2/h$), and total ammonia emissions were higher (p<0.05) in sawdust ($37.02mg/m^2$) than cocopeat India ($22.51mg/m^2$) and Vietnam ($13.60mg/m^2$). In Exp. II, an on-farm trial was conducted with 48 Hanwoo cattle in 16 pens using the same bedding materials as in Exp. I, with fan (blowing 2.00 m/s) and no fan treatments, and feed bunk side (FB) and water supply side (WS) within a pen (4.5 m, $width{\times}9.0m$, length). Beddings were replaced with fresh bedding materials when moisture concentrations were over 65%. No interactions among treatments were detected for moisture concentration and increment rates, and ammonia concentrations, but a significant effect was observed (p<0.01) for each of the treatments. Both concentrations and increment rate of moisture were higher (p<0.01) in the beddings without fan than with fan. Moisture concentrations and increment rate within a pen were also higher (p<0.01) in FB than WS. Thus, the whole no-fan-FB and sawdust-fan-FB were replaced with fresh bedding material between 4 to 5 experimental weeks. The ammonia concentrations and pH of beddings were not significantly different among treatments. Therefore, using cocopeat bedding with a blowing fan can extend twice the bedding utilization period, and WS within a pen showed twice the bedding-life compared to FB. Despite the outstanding characteristics of cocopeat compared with sawdust, using cocopeat as an alternative for sawdust bedding is not recommended for cattle management, considering it has 4.4 times higher bedding cost and a dust production problem.

흰점박이꽃무지 유충의 발육에 적합한 베리류톱밥 발효기간에 관한 연구

임주락,이상식,이은진,김웅,최창학 한국응용곤충학회 2023 한국응용곤충학회지 Vol.62 No.3

베리류톱밥 발효기간중 톱밥내 온도변화와 발효기간에 따른 흰점박이꽃무지 유충의 발육특성을 조사한 결과 참나무톱밥에 비해 발효속도가 빠르고, 발효기간 50~70일이 유충 발육에 가장 적합하였다. 발효기간중 톱밥내 온도변화는 발효초기에 베리류톱밥이 참나무톱밥이 40℃이하로 경과한데 반하여 베리류톱밥은 60℃ 이상 고온으로 경과되면서 빠르게 발효가 되었고, 60일 이내에 안정화 되었다. 개체사육에서 발효기간별 흰점박이꽃무지 유충의 총 발육기간은 베리류톱밥은 30일간 발효에서 104.8일로 가장 길고, 유의차가 인정되었으며, 50~90일 발효는 90일 정도로 차이가 없었다. 참나무톱밥도 베리류톱밥과 마찬가지 경향이나, 유충 발육기간이 30일정도 길었다. 유충 령기별 발육기간은 베리류톱밥과 참나무 톱밥에서 차이가 컸고, 유의차가 인정되었다. 톱밥 발효기간에 따른 유충 무게는 베리류톱밥과 참나무톱밥 모두 발효기간이 긴 90일 발효에서 가장 가볍고, 발효기간 30~70일 발효에서는 차이가 없었다. 생존율은 개체사육에서는 95% 이상으로 발효기간에 따른 차이는 없었으나, 집단사육에 서는 개체사육보다 생존율이 낮고, 발효기간 90일에서 가장 낮았다. 집단사육에서 출하기준인 2.5 g 이상 유충 비율은 베리류톱밥 발효기간 30~70일까지는 85% 정도로 차이가 없었지만, 발효기간 90일에서는 70%로 낮고, 유의차가 인정되었다. 참나무톱밥도 마찬가지 경향이며, 베리 류톱밥에 비해 10%정도 낮았다. 또한, 베리류톱밥은 40~60일이면 유충의 무게가 2.5 g 이상이 되었고, 참나무 발효톱밥은 75~105일이 걸렸다. After examining the temperature change in berry sawdust during the fermentation period and the developmental characteristics of Protaetia brevitarsis larvae according to the fermentation period, it was found that the fermentation speed was faster than with oak sawdust and a fermentation period of 50-70 days was the most suitable for larval growth. During the fermentation period, the temperature change in sawdust was quickly fermented as berry sawdust passed a high temperature of more than 60℃ in the early stages of fermentation compared to fermentation speed of oak sawdust, and stabilized within 60 days. In individual breeding, the total growth period of P. brevitarsis larvae by fermentation period was the longest at 104.8 days after fermentation for 30 days, which was a significant difference. There was no difference in the growth period between 50 and 90 days of fermentation. Oak sawdust tended to be the same as berry sawdust, but the larval development period was approximately 30 days. The developmental period by larval stage differed greatly between berries and oak sawdust. The weight of larvae according to the fermentation period of sawdust was the lowest after 90 days of fermentation, with a long fermentation period for both berry and oak sawdust, and there was no difference in the 30-70 days fermentation periods. The survival rate was more than 95% in individual breeding, and there was no difference among the fermentation periods. However, in group breeding, the survival rate was lower than that of individual breeding and was lowest at 90 days of fermentation. In group breeding, the ratio of larvae weighing ≥ 2.5 g, which is the standard for shipment, was 85% after 30-70 days of fermentation of berry sawdust, but was lower at 70% after 90 days of fermentation, which was a significant difference. The survival rate with oak sawdust showed the same trend, which was approximately 10% lower than that with sawdust. In addition, the larvae from the berry sawdust weighed > 2.5 g in 40-60 days, while it took 75-105 days with oak-fermented sawdust.

착유우의 톱밥발효우사 이용 연구 제2보 :착유우 톱밥발효우사의 톱밥상 처리방법에 따른 이용효과 비교

권두중,권응기,정석근,한정대,정석찬,강승원,강상열,정형섭,장학주 한국축산환경학회 1995 축산시설환경학회지 Vol.1 No.2

톱밥우사의 우상바닥처리 형태와 톱밥의 효율적 이용을 구명하기 위하여 PVC lightsheet지붕에 우상바닥처리별로 톱밥 10cm+콘크리트바닥, 톱밥 30cm+콘크리트바닥, 톱밥 30cm+바닥에 각각 경산우 6두(1두당 톱밥우상 16.5㎥)씩을 공시하여 시험한 결과 톱밥우상내 온도는 톱밥 30cm+흙바닥 처리가 가장 높았고, 수분증발량은 톱밥 10cm+콘크리트바닥에서 가장 많았다. 톱밥우사에서의 사료섭취 장소 및 톱밥우상의 분뇨배설은 단위면적당 톱밥우상에서는 91% 사료섭취장소에서는 9%가 배설되었으며, 착유우의 1일 분뇨 배설량은(착유시간 제외) 분 31.45kg, 뇨 10.7kg, 계 42.23kg이었으며 우분의 수분함량은 83.96%이었다. 공시완료한 분뇨가 혼합된 톱밥의 성분함량은 우분비율이 많은 톱밥 10cm+콘크리트바닥에서 무기물 성분이 가장 많이 함유되었으며, 흙바닥 처리구의 토양 중 NO$_3$-N함량이 허용한계 (10ppm이하)이상으로 검출되어 수질오염 가능성이 나타났다. 톱밥우상의 처리방법별 년간 유지비용은 톱밥 10cm+콘크리트바닥 처리가 년간 가장 적게 소요되었으며, 톱밥의 효율적 이용, 톱밥 교체시 노력절감 등으로 보아 가장 유리한 것으로 나타났다. This study was carried out to investigate the effect of depth of sawdust bedding and ground material in dairy cattle barn. Treatment included the concrete floor with a 10cm or 30cm sawdust and the earth floor with a 30cm sawdust. Eighteen cows were assigned to 3 pens with 16.5㎥/head bedding area. The results obtained were as follows : 1. The temperature of sawdust bed was highest in the earth floor with 30cm sawdust, and the moisture content of sawdust bed was highest in the concrete floor with 10cm sawdust bed. 2. Cows defecate feces 9.2, 8.7 and 9.3 times a day in 10cm sawdust on concrete floor(10S+C), 30cm sawdust on concrete floor(30S+C) and 30cm sawdust on non concrete floor(30S+ NC) respectively. In average, they excreta 9.1 times/day(85.8%) in the sawdust beds and 1.5 times/day(14.2%) in the feeding alley. 3. The ratio of daily water amount deposited vapor to total water amount deposited in sawdust beds was 74.0%, 61.5% and 47.1% in 10S+C, 30S+C and 30S+NC respectively. 4. N.P.K contents in the sawdust beds were higher for 10S+C compared with other treatments. 5. When 30cm of sawdust was applied on the earth ground NO$_3$-N contents in the sawdust bed was 37.7, 14.1 and 15.0ppm in depth of 30, 60 and 90cm under the ground, respectively, indicating some possibility of water pollution.

The effect of sawdust fermentation period and storage period after sawdust fermentation on the development of Protaetia brevitarsis larvae

임주락,Sang-Sik Lee,이은진,김웅,최창학 한국잠사학회 2024 International Journal of Industrial Entomology Vol.48 No.1

To investigate the effect of sawdust fermentation period and storage period after fermentation on development as food for P. brevitarsis larvae, individual and group breeding were conducted on berry sawdust and oak sawdust. In individual breeding, the growth period of P. brevitarsis larvae was reduced by 12 days from the 60-day fermentation of berry sawdust to the 40-day fermentation of berry sawdust, 30 days from the 90-day fermentation of oak sawdust, and the weight of the larvae was the heaviest. In group breeding, the time it takes for P. brevitarsis larvae to change from 1st to 3rd instar is about 30 days after hatching from 60-day fermentation of berry sawdust, while 90-day fermentation of oak sawdust took more than 60 days, so the growth speed was fast and the survival rate was good. The results of the farmhouse demonstration test were the same trend, and it was judged that it would be possible to produce P. brevitarsis larvae with berries fermented sawdust, and it would be advantageous in terms of economy. In addition, for both individual and group breeding, the growth period of P. brevitarsis larvae was longer as the storage period was longer, the weight of the larvae decreased, and the survival rate was no different. The development period of P. brevitarsis larvae was the longest in the storage period of 18 months for berries fermented sawdust, and the storage period of oak fermented sawdust was longer in the storage period of 12 months and 18 months. Therefore, considering the results of individual breeding and group breeding, the fermentation period was appropriate for 60 days for berries sawdust, and the storage period for berries sawdust was stable from 0 to 12 months, and 0 to 6 months for oak sawdust.

Thermophile mushroom cultivation in Cambodia : Spawn production and development of a new substrate, acacia tree sawdust

Hyun-You Chang,Youn-ju Huh,Pisey Soeun,Seung-ho Lee,Iva Song,Reaksmey Sophatt,Geum-Hui Seo 한국버섯학회 2016 한국버섯학회지 Vol.14 No.1

To minimize cultivation costs, prevent insect-pest infestation, and improve the production efficiency of thermophilic mushrooms, plant substrates obtainedfrom local areas in Cambodia were used for production of both spawn and mushrooms. In this experiment, different sawdusts different organic wastes and grain ingredients and analyzed for improvement of spawnproduction efficiency. Four thermophilic mushroom species, Pleurotus sajor-caju (oyster mushroom, Sambok), Ganoderma lucidum (deer horn shaped), Auricularia auricula (ear mushroom), and Lentinula edodes (shiitake), were used to identify efficient new substrates for spawn and mushroom production. Although the mycelia in the rubber tree sawdust medium showed a slightly slower growth rate (10.9 cm/15 days) than mycelia grown in grains (11.2 cm/15 days in rice seeds), rubber tree sawdust appeared to be an adequate replacement for grain spawn substrates. Th findings indicate that rubber tree sawdust, sugarcane bagasse, and acaciatree sawdust supplemented with rice bran and calcium carbonate could be new alternative the substrates for . Although sugarcane bagasse and rubber tree sawdust showed similarly high biological efficiencies (BE) of 60% and 60.8%, respectively, acacia tree sawdust exhibited relatively a low biological efficiency of 22.4%. However, it is expected that acacia sawdust has potential for the mushroom cultivation when supplemented with currently used sawdust substrates in Cambodia, because of its relatively low price. The price of the sawdust (20 kg sawdust= 6500 Riel or 1.6 USD) currently used was 6.5 times higher than the price of acacia sawdust (201000 Riel or 0.25 )USD). Therefore, utilization for acacia sawdust for mushroom cultivation could become feasible as it would reduce by producing costs of mushrooms in rural areas of Cambodia.

Thermophile mushroom cultivation in Cambodia: Spawn production and development of a new substrate, acacia tree sawdust

Chang, Hyun-You,Huh, Youn-ju,Soeun, Pisey,Lee, Seung-ho,Song, Iva,Sophatt, Reaksmey,Seo, Geum-Hui The Korean Society of Mushroom Science 2016 한국버섯학회지 Vol.14 No.1

이 연구는, 느타리버섯과 풀버섯만 재배하던 캄보디아에서 영지버섯, 목이버섯 그리고 표고버섯 등과 같은 여러 종류의 고온성 버섯들의 종균을 생산하고, 캄보디아에서 생산되는 경제적이고 효율적인 새로운 버섯배지를 찾기 위해 시도되었다. 종균 및 버섯재배 배지로, 여러 종류의 유기물찌꺼기, 곡물, 그리고 톱밥이 사용되었다. 고무나무 톱밥배지에서 자란 균사 (10.9 cm/15일)는 곡물배지에서 자란 균사 (11.2 cm/15일)에 비해 성장이 조금 느렸지만, 고무나무 톱밥은 낮은 가격 및 취급의 용이성이 있어, 비싼 곡물종균을 대치할 수 있는 매우 적절한 배지인 것으로 나타났다. 버섯생산을 위해서, 고무나무톱밥(60.8% BE), 사탕수수박(60% BE), 그리고 아카시아나무 톱밥 등에 미강과 탄산칼슘을 혼합하여 사용하였는데, 사탕수수박과 고무나무 톱밥은 매우 비슷한 높은 생물학적 효율(BE)을 보여주었고, 아카시아나무 톱밥은 비교적 낮은 22.4%의 생물학적 효율을 보여주었다. 따라서 낮은 가격에 구입할 수 있는 아카시아톱밥이 현재 사용되고 있는 높은 가격의 배지들과 혼합하여 사용된다면 매우 가능성이 높은 새로운 배지가 될 수 있을 것으로 보였다. 현재, 사용되고 있는 톱밥의 가격 (20 kg 고무나무톱밥=6500 Riel 혹은 1.6 USD)과 비교했을 때, 아카시아톱밥의 가격(20 kg=1000Riel 혹은 0.25USD)이 매우 낮아 버섯의 생산에 투자되는 비용을 크게 줄여 경제성을 높일 수 있을 것으로 사료된다. To minimize cultivation costs, prevent insect-pest infestation, and improve the production efficiency of thermophilic mushrooms, plant substrates obtained from local areas in Cambodia were used for production of both spawn and mushrooms. In this experiment, different sawdusts different organic wastes and grain ingredients and analyzed for improvement of spawn-production efficiency. Four thermophilic mushroom species, Pleurotus sajor-caju (oyster mushroom, Sambok), Ganoderma lucidum (deer horn shaped), Auricularia auricula (ear mushroom), and Lentinula edodes (shiitake), were used to identify efficient new substrates for spawn and mushroom production. Although the mycelia in the rubber tree sawdust medium showed a slightly slower growth rate (10.9 cm/15 days) than mycelia grown in grains (11.2 cm/15 days in rice seeds), rubber tree sawdust appeared to be an adequate replacement for grain spawn substrates. Th findings indicate that rubber tree sawdust, sugarcane bagasse, and acacia tree sawdust supplemented with rice bran and calcium carbonate could be new alternative the substrates for. Although sugarcane bagasse and rubber tree sawdust showed similarly high biological efficiencies (BE) of 60% and 60.8%, respectively, acacia tree sawdust exhibited relatively a low biological efficiency of 22.4%. However, it is expected that acacia sawdust has potential for the mushroom cultivation when supplemented with currently used sawdust substrates in Cambodia, because of its relatively low price. The price of the sawdust (20 kg sawdust= 6500 Riel or 1.6 USD) currently used was 6.5 times higher than the price of acacia sawdust (201000 Riel or 0.25 USD). Therefore, utilization for acacia sawdust for mushroom cultivation could become feasible as it would reduce by producing costs of mushrooms in rural areas of Cambodia.

Thermophile mushroom cultivation in Cambodia: Spawn production and development of a new substrate, acacia tree sawdust

Hyun-You Chang,Youn-ju Huh,Pisey Soeun,Seung-ho Lee,Iva Song,Reaksmey Sophatt,Geum-Hui Seo 한국버섯학회 2016 한국버섯학회지 Vol.14 No.1

To minimize cultivation costs, prevent insect-pest infestation, and improve the production efficiency of thermophilic mushrooms, plant substrates obtainedfrom local areas in Cambodia were used for production of both spawn and mushrooms. In this experiment, different sawdusts different organic wastes and grain ingredients and analyzed for improvement of spawnproduction efficiency. Four thermophilic mushroom species, Pleurotus sajor-caju (oyster mushroom, Sambok), Ganoderma lucidum (deer horn shaped), Auricularia auricula (ear mushroom), and Lentinula edodes (shiitake), were used to identify efficient new substrates for spawn and mushroom production. Although the mycelia in the rubber tree sawdust medium showed a slightly slower growth rate (10.9 cm/15 days) than mycelia grown in grains (11.2 cm/15 days in rice seeds), rubber tree sawdust appeared to be an adequate replacement for grain spawn substrates. Th findings indicate that rubber tree sawdust, sugarcane bagasse, and acaciatree sawdust supplemented with rice bran and calcium carbonate could be new alternative the substrates for . Although sugarcane bagasse and rubber tree sawdust showed similarly high biological efficiencies (BE) of 60% and 60.8%, respectively, acacia tree sawdust exhibited relatively a low biological efficiency of 22.4%. However, it is expected that acacia sawdust has potential for the mushroom cultivation when supplemented with currently used sawdust substrates in Cambodia, because of its relatively low price. The price of the sawdust (20 kg sawdust= 6500 Riel or 1.6 USD) currently used was 6.5 times higher than the price of acacia sawdust (201000 Riel or 0.25 )USD). Therefore, utilization for acacia sawdust for mushroom cultivation could become feasible as it would reduce by producing costs of mushrooms in rural areas of Cambodia.

Mechanical Performances of Boards Made from Carbonized Rice Husk and Sawdust: The Effect of Resin and Sawdust Addition Ratio(왕겨숯과 톱밥을 이용하여 제조한 보드의 역학적 성능: 수지 및 톱밥첨가량의 영향)

( Jung-woo Hwang ),( Seung-won Oh ) 한국목재공학회 2020 목재공학 Vol.48 No.5

A board was manufactured for each resin and sawdust addition using the chaff made by carbonizing the chaff charcoal, an agricultural by-product that emerge during the rice pounding process, and sawdust. And effects of the additions of resin and sawdust on coefficients of dynamic and static modulus of elasticity, modulus of rupture, as well as the relationship between the dynamic modulus of elasticity, statis modulus of elasticity, and modulus of rupture were investigated. As phenol resin addition of chaff charcoal-sawdust compound boardincreases to 10~25%, the bending performance has increased. This suggests that resin addition largely effects the bending performance. Although the bending performance was gradually increased with the increase in sawdust addition, since the coefficients of determination (R²) between the sawdust addition with the coefficients of dynamic, static modulus of elasticity, and modulus of rupture were 0.4012, 0.0809, and 0.1971, respectively. Thus, it showed a relatively lower correlation, and the effect of sawdust on bending performance was small. Since a high correlation was confirmed between dynamic and static modulus of elasticity, and modulus of rupture of chaff charcoal-sawdust compound board, it was confirmed that prediction of static modulus of elasticity and modulus of rupture can be made in a nondestructive way from the dynamic modulus of elasticity.

느타리 톱밥종균 배지조성에 따른 생산성 비교

최종인,최준영,김정한,구옥,김연진,하태문,정구현,Choi, Jong-In,Choi, Jun-Yeong,Kim, Jeong-Han,Gu, Ok,Kim, Yeon-Jin,Ha, Tai-Moon,Jung, Gu-Hyun 한국버섯학회 2021 한국버섯학회지 Vol.19 No.1

느타리 종균배지재료인 미송톱밥과 미강을 대체할 수 있는 재료를 선발하기 위하여 실시하였다. 톱밥의 pH는 4.9~6.1, 총질소함량은 0.21~0.26%, 총탄소함량은 49.0~50.2%를 나타내며 조지방은 0.3~0.7%를 함유하고 있다. 종균 혼합배지의 pH는 5.3~5.9, 총질소함량은 0.65~1.11%, 총탄소함량은 47.0~49.1%를 나타내었다. C/N율은 총질소 함량이 낮은 미루나무톱밥 혼합배지에서 높게 나타났으며, 단백질 함량은 미송톱밥이 함유되어 있는 배지에서 높게 나타났다. 느타리 균사생장은 미강보다 밀기울이 포함되어 있는 배지에서 균사생장 속도가 빨랐다. 수량은 '곤지7호', '춘추2호', '흑타리', '수한1호' 순으로 높았으며, T4(미루나무톱밥:참나무톱밥:미강) 배지에서 가장 높은 생산성을 나타내었으나 처리 간 유의성이 없었다. 느타리 농가에서는 자가종균 제조 시 주변에서 손쉽게 구할 수 있는 미루나무톱밥, 참나무톱밥을 단용 및 혼용으로 이용할 수 있으며, 보관중에 품질유지가 어려운 미강 대신에 밀기울을 사용하여 종균을 제조할 수 있을 것으로 판단된다. This study was performed to find a medium material that can replace Douglas fir sawdust and rice bran in spawn media for growing spawn directly in the oyster mushroom farm. The pH range, total nitrogen source, and total carbon source of the mixed spawn medium were 5.3~5.9, 0.65~1.11%, and 47.0~49.1%, respectively. The C/N ratio was high in the mixed medium of poplar sawdust, with a low total nitrogen content. The protein content was high in the medium containing fermented Douglas fir sawdust. The mycelium growth rate was higher in the medium containing wheat bran than that in the medium containing rice bran. The highest yield per bottle was observed with poplar sawdust, oak sawdust, and rice bran mixed at a ratio of 40:40:20 (v/v/v); however, there was no significant difference observed in terms of productivity with the other treatments. Thus, when growing sawdust spawns in farms, it is efficient to use poplar sawdust, oak sawdust, and wheat bran, which are also easily available, instead of Douglas fir sawdust and rice bran.