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하태문,지정현,주영철,성재모 한국버섯학회 2006 한국버섯학회지 Vol.4 No.4
We have investigated cultural circumstance and given condition of king oyster mushroom(Pleurotus eryngii) growing farmer. We collected many pathogens from King oyster mushroom growing farmer and identified with chemicobiological test and microscope. Most of investigated farmers neglected their`s growing room cleaning and washing, after harvesting At pin-heading induction time, humidity degree in growing room was kept of high level and Air ventilation volume was so little that fruit-body formation ratio was low. The collected pathogens were twenty eight strains and identified with Pseudomonas sp., Trichoderma sp. mostly. During the spawn running time and pin-heading induction time, contamination by Trichoderma sp. occurred mostly, but during the fruit-body growing time, contamination by Pseudomonas sp., Erwinia sp. etc, occurred
하태문,최종인,전대훈,주영철,신평균 한국버섯학회 2013 한국버섯학회지 Vol.11 No.2
A new variety of Pleurotus eryngii which is named ‘Gonji No. 8’ was bred by mating monokaryotic strain isolated from E085D2 and a monokaryotic strain ‘aerini No.3’ obtained from the Mushroom Research Institute, Gyeonggi-Do A.R.E.S. The characteristics of the new variety `Gonji No.8` is as follows. The optimum temperature for mycelium growth was from 23 oC to 26 oC on potato dextrose agar (PDA) medium. For the primodia formation and the growth of fruit bodies, the optimum temperature was from 14 oC to 16 oC. The period of spawn running was around 30 days at 22 oC and the days taken after the removal of the spawn layer to initiate primodia was seven days. The hardness value of fruit body was 8,4322,193 g/cm2, which was two times more than that of ‘Keunneutari No.2’. The yield of ‘Gonji No.8’ was about 133 g per bottle(900cc) and it was same as ‘Keunneutari No.2’.
하태문,주영철,전대훈,최종인,이태수 한국버섯학회 2011 한국버섯학회지 Vol.9 No.1
We bred a new strain of Pleurotus eryngii. It’s name is ‘Gongi No.3’ and it was bred by mating monokaryotic strain isolated from E08-5D2 and dikaryotic strain GMPE25016 from 2006 to 2010 in Mushroom Research Station, Gyonggi province A.R.E.S. The characteristics of a new strain ‘Gongi No.3’ is as follows ; The optimum temperature for mycelium growth was from 26 to 29 degrees celsius on PDA medium and those for the premodium formation and the growth of fruit body were from 14 to 18 degrees celsius. The period of spawn running was around 30days at 22 degrees celsius and the period taken from scratching old spawn to make premodium were 8 days. The color degree of cap surface was measured by color difference meter and that of a new strain ‘Gongi No.3’ was 54.4 by L-value. it was seem to be dark, compared with ‘Keunneutari No.2’. The hardness of fruit body of a new strain was higher than ‘Keunneutari No.2’. The yield was about 180g per bottle(1100cc). it was 10g more than ‘Keunneutari No.2’.
느타리 균상 폐면배지 야외발효 조건별 이화학적 특성 변화
하태문,주영철,지정현,김영호,성재모 한국버섯학회 2005 한국버섯학회지 Vol.3 No.3
we studied on chemical & physical variation of cotton waste according to covering materials during outdoor fermentation for oyster mushroom bed culture with cotton waste medium. P.E vinyl and nonwoven thin cloth were used for the covering material. Temperate in the medium ascended up to 75℃ in treatment using nonwoven thin cloth but 60℃ in P.E vinyl treatment for fifth day to seventh day, after that time fell slowly. Before the fermentation, pH of cotton waste medium was 7.2∼7.5 but during the fementation, pH in using nonwoven thin cloth treatment was about 8.9 and pH in P.E vinyl fell 5.1. Ammonium ion concentration in cotton waste medium was 70-85ppm in treatment using P.E vinyl and higher than nonwoven thin cloth. Total carbon content of cotton waste medium was increased but total nitrogen content was decreased while fermentation make progress. when we investigated cotton texture wholly femented by SEM & TEM, cell wall of cotton in treatment using nonwoven thin cloth mostly degraded but it in P.E vinyl was kept unimpaired
품종 및 재배지역별 과채류와 과실류의 유리당 함량 비교 분석
하태문,원선이,서재순,강희윤,이대형,권영희,Ha, Tai-Moon,Won, Seon-Yi,Seo, Jaesoon,Kang, Heui-Yun,Lee, Dae-Hyoung,Keuyn, Young-Hee 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.4
The objective of this study was to analyze the free sugar contents of ten kinds of fruits and fruit vegetables that were collected from many district in Korea. The analyzed results will be used as basic nutrition component reference to processing. The results were summarized as follows. The total free sugar contents among the ten kinds of fruit vegetables was highest in grape. The contents of glucose, fructose and sucrose were 6.39%, 6.27%, 0.28%, respectively. The average total sugar contents of four cultivar of apple was 11.42% and average fructose contents was 6.13%. The free sugar contents of thirteen samples of the same cultivar of apple were all differing with statistical significance. The average total free sugar contents of eight cultivar of peach was 8.66%. In case of tomato, glucose content was approximately 1.35~2.83% and fructose was 1.26~2.51%. The contents of glucose, fructose and sucrose in paprika were 2.19%, 2.06%, 0.1%, respectively.
큰느타리버섯 재배실태조사와 기형버섯 발생경감에 관한 연구 Ⅱ. 기형버섯 발생경감 재배기술
하태문,주영철,신평균 한국버섯학회 2011 한국버섯학회지 Vol.9 No.2
We have researched on methods which can reduce fruit-body malformations of king oyster mushroom (Pleurotus eryngii). We collected many pathogens from diseased fruit-body or malformated fruit-body and identified with chemicobiological test and microscope. The factors of fruit-body malformations and increment of contamination during the pin- heading induction time were researched with ventilation amounts in growing room. When the pathogens having high pathogenicity were inoculated in spawn running bottle and at pin-heading induction time, symptom appeared or not appeared in according to air ventilation amounts in growing room. During the pin-heading induction time, humidity degree in growing room have kept of high level and air ventilation amounts were so little that fruit-body malformations ratio was high. But, even though pathogens were inoculated at the surface of bottle factitiously, if air ventilation amounts were enough, fruit-body malformation ratio was low.
하태문,정구현,김정숙,최종인,김정한,이용선,정윤경,Ha, Tai-Moon,Jung, Gu-Hyun,Kim, Jeoung-Suk,Choi, Jong-In,Kim, Jeong-Han,Lee, Yong-Seon,Jeong, Yung-Kyeoung 한국버섯학회 2021 한국버섯학회지 Vol.19 No.2
국내육성 품종의 부족과 자실체 발생이 불안정한 단점을 보완하고 병해에 강한 백령버섯 신품종 육성을 위해 2015년부터 국내외에서 유전자원을 수집하고 특성검정 후 교배, 특성검정, 생산력 검정, 농가실증 등의 과정을 통해 육성한 백령버섯 신품종 '우람'의 주요 특성은 다음과 같다. 균사생장적온은 26~29℃, 발이 및 생육온도는 15~18℃, 갓형태 평편형, 갓색 백색으로 대조품종(KME 65035)과 유사하였다. 초발이소요일수는 병재배 시 5일, 봉지재배 시 6일로 대조품종보다 2~4일 짧았다. 대직경은 32.6~37.0 mm로 대조품종 44.3~53.1 mm보다 작았으나, 자실체장은 130.4 mm로 대조품종 114.8~115.2 mm보다 길었다. 자실체 유효경수는 병재배 시 1.8개, 봉지재배 시 2.9개로 대조품종보다 많았다. 발이율은 93.3~100%로 대조품종 보다 안정적이었다. 병재배 및 봉지재배 시 수량은 각각 173.1 g/병(1,100cc), 283.4 g/봉지(1.2 kg)으로 대조품종 138.0 g/병(1100cc), 197.4 g봉지(1.2 kg) 보다 25~44% 높았다. 모본 및 대조품종과 대치 배양시 대치선이 뚜렷하였고, 균사체 DNA PCR반응 결과, 밴드패턴이 모본 및 대조품종과 다른 양상을 보여 교배종임을 확인할 수 있었다. This study was carried out to breed new variety of Pleurotus nebrodensis. We have collected and tested characteristics of genetic resources from domestic and abroad since 2015. The varieties of P. nebrodensis from China are grown by farmers, but those have been unstable fruiting and are weak against bacterial diseases. To solve this problem, we bred the unique domestic variety 'Uram' of P. nebrodensis and the results of the characteristic test for the new 'Uram' are as follows. The proper temperature for the mycelial growth was 26~29℃ and fruit body growth temperature was 15~18℃. It was similar to the control variety KME65035 of P. nebrodensis in the pileus form of a flat and white color. The number of days required for initial fruting was 5 days for bottle cultivation and 6 days for bag cultivation which was 2-4 days shorter than that of the control variety. The pileus diameter was 32.6-37.0 mm which was smaller but the fruit body length was 130.4 mm, which was longer than those of the control variety. The effective number of fruit bodies was 1.8 in bottle cultivation and 2.9 in bag cultivation, which was more than those of the control variety. The yield rate was 93.3-100%, which was more stable than those of the control variety. In bottle cultivation and bag cultivation, the yield was 173.1 g/bottle (1100 cc) and 283.4 g/bag (1.2 kg), respectively, which was 25-44% higher than those of the control variety 138.0 g/bottle (1100 cc) and 197.4 g bag (1.2 kg). When incubating the parent and control varieties of 'Uram', the replacement line was clear and as a result of mycelial DNA RAPD-PCR reaction, the band pattern was different from that of the parent and control varieties, confirming the hybrid species.