Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations

반재준,정진영,임우석,김만호,김세훈 대한수의학회 2012 Journal of Veterinary Science Vol.13 No.4

Adipose-derived stem cells (ASCs) are believed to have potential use for treating many illnesses. Most cells, including ASCs, are generally cultured in medium containing fetal bovine serum (FBS). However, FBS, which could induce an immune response or infection, is not recommended for clinical applications. In the present study, we evaluated the morphology, proliferation rate, and characterization of rabbit ASCs grown in medium containing autologous serum (AS) and compared these cells to ones cultured with FBS. Morphological changes were monitored by microscopy and flow cytometry. Proliferation rates were assessed with cell counting and ASC phenotypes were characterized by flow cytometry using representative surface markers (CD44 and CD45). Expression of epidermal growth factor, brain-derived neurotrophic factor, and vascular endothelial growth factor was measured by reverse transcription-polymerase chain reaction. Results of our study showed that ASCs had a greater expansion rate in AS without developing morphological heterogeneity than cells grown in FBS. AS-cultured ASCs expressed representative growth factors, CD44 but not CD45, similar to cells cultured in FBS. Expression levels of some growth factors were different between AS and FBS. In conclusion, our findings indicated that AS could potentially be used as a culture medium supplement for the expansion of autologous ASCs.

Synergistic Effect of Carboxymethyl Chitosan and Adipose-Derived Mesenchymal Stem Cells on Osteoarthritis Model in Rabbits

김지훈,윤성호,서민수,배슬기,장민,구새광,권영삼,이해범 한국임상수의학회 2020 한국임상수의학회지 Vol.37 No.5

The purpose of this study is to investigate the clinical effects of carboxymethyl chitosan (CMC) and adiposederived mesenchymal stem cells (MSCs) on osteoarthritis (OA). Thirty New Zealand white rabbits were used as cranial cruciate ligament transection and partial meniscectomy models. The rabbits were divided into five groups (n = 6) according to the intra-articular injection materials: the control group with PBS, the HA group with hyaluronic acid, the CMC group with CMC, the MSC group with MSCs emerged in PBS, and the MSC+CMC group with CMC and MSCs. Knee thickness, extension angle, gross morphology, histopathology and immunohistochemistry were performed to evaluate the effects of CMC and MSCs on rabbit OA. On the morphologic and histologic examination, the articular surfaces of the femur and tibia were markedly damaged in control group with higher Mankin score and lower cartilage surface thickness. However, OA related cartilage defects were alleviated by the treatment of MSC and/ or CMC. The expressions of apoptotic and inflammatory cytokines were decreased and cartilage extracellular matrix (ECM) related collagens I and II were enhanced by the treatment of MSC and/or CMC. In conclusion, this study showed that CMC and MSC treatments have a beneficial effects on OA via the protection of cartilage damage, the stimulation of ECM, and the inhibition of inflammatory and apoptotic reaction.

Effect of lactoferrin-impregnated porous poly(lactide-co-glycolide) (PLGA) microspheres on osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs)

Kim, S.E.,Yun, Y.P.,Shim, K.S.,Park, K.,Choi, S.W.,Suh, D.H. Elsevier 2014 Colloids and Surfaces B Vol.122 No.-

The aim of this study was to develop lactoferrin (LF)-impregnated porous poly(lactide-co-glycolide) (PLGA) microspheres (PMs) to induce osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). Porous PLGA PMs were fabricated by a fluidic device and their surfaces were modified with heparin-dopamine (Hep-DOPA). Then, LF (100μg, 500μg, and 1000μg) was impregnated on the surface of heparinized PMs (Hep-PMs) via electrostatic interactions to yield LF-impregnated PMs. PMs and modified PMs were characterized by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Osteogenic differentiation of rADSCs on PMs and modified PMs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and mRNA expression of osteocalcin and osteopontin. Successful immobilization of Hep-DOPA and LF on the surface of PMs was confirmed by XPS analysis. LF-impregnated PMs generated significantly greater ALP activity, calcium deposition, and mRNA expression of osteocalcin and osteopontin compared with PMs. These results suggested that LF-impregnated PMs effectively induced osteogenic differentiation of rADSCs.

Osteogenesis and new bone formation of alendronate-immobilized porous PLGA microspheres in a rat calvarial defect model

Lee, Jae Yong,Kim, Sung Eun,Yun, Young-Pil,Choi, Sung-Wook,Jeon, Daniel I.,Kim, Hak-Jun,Park, Kyeongsoon,Song, Hae-Ryong Elsevier 2017 Journal of industrial and engineering chemistry Vol.52 No.-

<P><B>Abstract</B></P> <P>In this study, we evaluated <I>in vitro</I> osteogenic differentiation and <I>in vivo</I> new bone formation using the alendronate-releasing porous PLGA microsphere (Aln/PMS) system in rats with a critical-sized calvarial defect. Aln/PMS system significantly enhanced <I>in vitro</I> osteogenic differentiation and maturation, showing significantly enhanced ALP activity, calcium deposition and the expression of osteocalcin and osteopontin, relative to PMS alone. Also, micro-CT analyses and histology results suggested that Aln/PMS system increased mineralization and bone matrix formation compared to PMS alone. This study demonstrated that the local delivery of Aln, a potent-osteoinductive factor, significantly enhances rabbit adipose-derived stem cells osteogenesis and bone regeneration.</P>

Mechanistic target of rapamycin and an extracellular signalingregulated kinases 1 and 2 signaling participate in the process of acetate regulating lipid metabolism and hormone-sensitive lipase expression

Li Yujuan,Fu Chunyan,Liu Lei,Liu Yongxu,Li Fuchang 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.9

Objective: Acetate plays an important role in host lipid metabolism. However, the network of acetate-regulated lipid metabolism remains unclear. Previous studies show that mitogen-activated protein kinases (MAPKs) and mechanistic target of rapamycin (mTOR) play a crucial role in lipid metabolism. We hypothesize that acetate could affect MAPKs and/or mTOR signaling and then regulate lipid metabolism. The present study investigated whether any cross talk occurs among MAPKs, mTOR and acetate in regulating lipid metabolism.Methods: The ceramide C6 (an extracellular signaling-regulated kinases 1 and 2 [ERK1/2] activator) and MHY1485 (a mTOR activator) were used to treat rabbit adipose-derived stem cells (ADSCs) with or without acetate, respectively.Results: It indicated that acetate (9 mM) treatment for 48 h decreased the lipid deposition in rabbit ADSCs. Acetate treatment decreased significantly phosphorylated protein levels of ERK1/2 and mTOR but significantly increased mRNA level of hormone-sensitive lipase (HSL). Acetate treatment did not significantly alter the phosphorylated protein level of p38 MAPK and c-Jun aminoterminal kinase (JNK). Activation of ERK1/2 and mTOR by respective addition in media with ceramide C6 and MHY1485 significantly attenuated decreased lipid deposition and increased HSL expression caused by acetate.Conclusion: Our results suggest that ERK1/2 and mTOR signaling pathways are associated with acetate regulated HSL gene expression and lipid deposition.

Osteogenesis and new bone formation of alendronate-immobilized porous PLGA microspheres in a rat calvarial defect model

이재용,김성은,윤영필,최성욱,Daniel I. Jeon,김학준,박경순,송해룡 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.52 No.-

In this study, we evaluated in vitro osteogenic differentiation and in vivo new bone formation using thealendronate-releasing porous PLGA microsphere (Aln/PMS) system in rats with a critical-sized calvarialdefect. Aln/PMS system significantly enhanced in vitro osteogenic differentiation and maturation,showing significantly enhanced ALP activity, calcium deposition and the expression of osteocalcin andosteopontin, relative to PMS alone. Also, micro-CT analyses and histology results suggested that Aln/PMSsystem increased mineralization and bone matrix formation compared to PMS alone. This studydemonstrated that the local delivery of Aln, a potent-osteoinductive factor, significantly enhances rabbitadipose-derived stem cells osteogenesis and bone regeneration.