급성심근경색증의 조기진단에 있어서 Myoglobin의 진단적 의의

이정헌,김종근,정병천,서강석,박정배,조용근,류재근,전재은,채성철,박의현 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: Exact and early diagnosis of acute myocardial infarction (AMI) is essential for the subsequent routine management of this frequent cardiovascular disease. Currently, AMI has been diagnosed using the combination of the history, electrocardiogram(ECG), and biochemical markers of myocardial necrosis. At present, many biochemical markers are used to diagnose AMI. In this study, the predictive values of serum myoglobin and creatine kinase-MB(CK-MB) were compared in the emergency department. Methods: Fifty-four consecutive patients who presented within 12 hours from onset of chest pain of presumed cardiac origin were enrolled into the study. Patients with trauma or renal failure were excluded. The serial serum myoglobin and CK-MB levels were obtained prospectively at admission and 2, 4, 6, 12, 24 and 48 hours after admission. We compare the levels of serum myoglobin and CK-MB within 2, 4, 6, 8, 10, 12, 24, and 48 hours after symptom onset respectively. And we compare the sensitivity, specificity, positive predictive value, and negative predictive value of myoglobin and CK-MB. Results: using World Health Organization criteria, 28 AMI patients were identified. Mean time from symptom onset to presentation was 4.1±1.3 hours. The predictive values of serum myoglobin were better than those of CK-MB within 6 hours after symptom onset. But, 6 hours after symptom onset, the predictive values of CK-MB were better than those of serum myoglobin. The false positive cases of serum myoglobin were 3-one was lung cancer with pleural effusion and the others were unstable angina. The false positive cases of CK-MB were 6-one case was viral myocarditis and the ohters were unstable angina. Conclusion: To compare the serum myoglobin and CK-MB in the diagnosis of AMI, serum myoglobin had better predictive values than CK-MB within 6 hours after symptom onset and useful in the early diagnosis of AMI. But, 6 hours after symptom onset, CK-MB had better predictive values than serum myoglobin and useful in following up.

불안정형 관동맥질환 환자에서 주요 심혈관 사건의 예측 인자로서의 심근 Troponin 1 및 Myoglobin 에 관한 연구

허재웅(Jae Woong Hur),김경현(Kyung Hyun Kim),하영준(Young Jun Ha),박찬수(Chan Su Park),서우종(Woo Jong Seo),조영재(Yeong Jae Cho),차경우(Keung Woo Cha),남시현(Si Hyun Nam),김종연(Jong Yeon Kim) 대한내과학회 2001 대한내과학회지 Vol.61 No.5

Background: Patients with unstable angina or non Q wave myocardial infarction still have an elevated risk for subsequent cardiac events. Therefore early assessment of the risk of future cardiac events is important. In this study, prognostic value of troponin I and myoglobin was evaluated and compared with other known early available risk indicators. Methods: Serum troponin I and myoglobin were measured at presentation and 8 hours, 16 hours, and 24 hours thereafter in 126 patients with unstable angina (n=70) or non Q myocardial infarction (n=56) from Jan 1998, through Feb 2000. Echocardiography was performed with calculation of wall motion score index. The incidence of cardiac death or myocardial infarction was compared between patients with normal troponin I, myoglobin and abnormal ones, respectively. Results: (1) At 6 months, 1 death (2.2%) and 1 myocardial infarction (2.2%) occurred in the 45 unstable angina patient s with normal troponin I compared with 3 deaths (12.0%) and 8 myocardial infarctions (32.0%) in the 25 unstable angina patient s with elevated troponin I. (2) At 6 months, 1 death (1.7%) and 3 myocardial infarctions (5.2%) occurred in the 58 unstable angina patients with normal myoglobin compared with 3 deaths (25.0%) and 6 myocardial infarctions (50.0%) in the 12 unstable angina patient s with elevated myoglobin. (3) When the analysis was limited to patients with normal baseline troponin I (≤0.4 ng/ dl: n=45), increased myoglobin level remained significantly associated with increased incidence of cardiac deaths or myocardial infarction compared with normal myoglobin level (16.7% vs 0%). (4) Together with age, diabetes, wall motion score index, troponin I and myoglobin levels were identified as independent prognostic variables for myocardial infarction or cardiac death in a multivariate logistic regression analysis. (5) The maximal troponin I and myoglobin value obtained during the first 24 hours provides independent and import ant prognostic information regardless of whether the patient is classified as having unstable angina or non Q wave myocardial infarction. Conclusion: The maximum troponin I and myoglobin obtained during the first 24 hours provides independent and important prognostic information in patients with unstable angina or non Q wave myocardial infarction. (Korean J Med 61:496- 505, 2001)

부동스트레스에 의한 심방근육세포막 투과성 변화의 형태학적 관찰

신해영(Hae Young Shin),송창호(Chang Ho Song),한인혁(Eui-Hyeog Han),손장신(Jang Sihn Sohn),박춘수(Chun Soo Park),이무삼(Moo Sam Lee) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.4

부동스트레스가 심방근육세포의 막투과성에 미치는 영향을 알아보기 위하여, 흰쥐를 6시간, 12시간, 24시간동안 플라스 틱관에 넣어 스트레스를 준 후 면역조직화학적 방법으로 심방근육세포내 myoglobin과 α-actinin의 변화를 관찰하고, 전자 현미경으로 lanthanum 추적법을 이용하여 심방근육세포의 막투과성을 관찰하였다. 심방근육세포내 myoglobin과 α-actinin의 변화 부동스트레스 6시간군과 12시간군에서는 심방근육세포내 myoglobin과 α-actinin의 양성반응이 대조군에 비하여 약하였다. 부동스트레스 24시간군에서는 myoglobin과 α-actinin 반응이 12시간군에 비하여 부분적으로 증가하는 경향이었으나 대조군 수준보다는 약하였다. Lanthanum (La) 추적법에 의한 전자현미경 관찰대조군에서 La는 심방근육세포의 세포사이공간에만 침착되었다. 부동스트레스 6시간군에서는 심방근육세포의 미세구조적 변화를 보였으며, La는 심방근육세포내 근육원섬유와 사립체 주위, 사립체 바탕질에 침착되었다. 부동스트레스 12시간군과 24시간군에서도 약간의 심방근육세포의 형태학적 변화를 보였으며, La는 부동스트레스 12시간군에서 심방근육세포의 근육원섬유와 사립체 주위, 사립체 바탕질에 침착되었으나, 부동스트레스 24시간군에서는 주로 심방근육세포의 세포사이공간에 침착되었고, 드물게 근육형질에서도 관찰되었다. 위의 결과들은 부동스트레스가 심방근육세포의 막투과성과 미세구조에 영향을 미친다는 것을 보여준다. This study was performed to investigate the effect of immobilization stress on the ultrastructural changes and membrane permeability in rat atrial myocyte using immunohistochemical and lanthanum tracer techniques. Male Sprague-Dawley rats, body weight 160~200 g, were used for all immobilization stress group. Rats were immobilized in small round plastic tube for 6, 12 or 24 hours, except for the control group. Alterations of myocardial myoglobin and α-actinin as well as membrane permeability after immobilization stress were examined by immunohistochemistry, and lanthanum permeability of the rat atrial myocyte were observed by electron microscopy. In the control group, there was no loss of myoglobin or α-actinin from the atrial myocytes. After 6 and 12 hours immobilization stress, the loss of myoglobin and α-actinin could be identified the atrial myocytes. In the 24 hour immobilization groups, the content of the myoglobin and α-actinin recovered partially. Lanthanum was deposited only in the intercellular space of the atrial myocardium in the control group. In the 6 hour immobilization group, the atrial myocytes showed severe ultrastructural changes during immobilization stress. Lanthanum deposited in the sarcoplasm, myofibrils, adjacent of mitochondria, and mitochondrial matrix. In the 12 or 24 hour immobilization groups, the morphological alteration of atrial myocytes appeared weekly. In the 12 hour group, lanthanum deposited in myofibrils, adjacent of mitochondria and in the mitochondrial matrix. In the 24 hour group, lanthanum deposited mainly in intercellular space of atrial myocardium, and rarely in the sarcoplasm of myocytes. These results suggest that the immobilization stress may induce the alteration of cardiac cell membrane permeability and the ultrastructures of atrial myocardium.

Identification of peptides that selectively bind to myoglobin by biopanning of phage displayed-peptide library

Padmanaban, G.,Park, H.,Choi, J.S.,Cho, Y.W.,Kang, W.C.,Moon, C.I.,Kim, I.S.,Lee, B.H. Elsevier Science Publishers 2014 Journal of biotechnology Vol.187 No.-

Biopanning of phage displayed-peptide library was performed against myoglobin, a marker for the early assessment of acute myocardial infarction (AMI), to identify peptides that selectively bind to myoglobin. Using myoglobin-conjugated magnetic beads, phages that bound to myoglobin were collected and amplified for the next round of screening. A 148-fold enrichment of phage titer was observed after five rounds of screening relative to the first round. After phage binding ELISA, three phage clones were selected (3R1, 3R7 and 3R10) and the inserted peptides were chemically synthesized. The analysis of binding affinity showed that the 3R7 (CPSTLGASC) peptide had higher binding affinity (K<SUB>d</SUB>=57nM) than did the 3R1 (CNLSSSWIC) and 3R10 (CVPRLSAPC) peptide (K<SUB>d</SUB>=125nM and 293nM, respectively). Cross binding activity to other proteins, such as bovine serum albumin, troponin I, and creatine kinase-MB, was minimal. In a peptide-antibody sandwich ELISA, the selected peptides efficiently captured myoglobin. Moreover, the concentrations of myoglobin in serum samples measured by a peptide-peptide sandwich assay were comparable to those measured by a commercial antibody-based kit. These results indicate that the identified peptides can be used for the detection of myoglobin and may be a cost effective alternative to antibodies.

인체에 적용한 전기자극이 Serum myoglobin과 Aldolase에 미치는 영향에 대한 생리학적 효과

김순희,천기영,최영덕,Kim, Soon-Hee,Chon, Ki-Young,Choi, Young-Deok 대한물리치료과학회 1999 대한물리치료과학회지 Vol.6 No.3

Prior studies have revealed that several stimulation to the muscle have released serum myoglobin into the blood vessel and increased aldolase activity. The present authors carried out a study which effect of electrical stimulation treatment (induced a isotonic wrist exerceise) on serum myoglobin(Mb) levels and aldolase(Al) activity were investigated in 6 healthy female. There were four groups of female: 1. no electrical stimulation control: 2. electrical stimulation 10min (EST10'); 3. electrical stimulation 20 min (EST20'); 4. electrical stimulation 30min (EST30'). Each groups is all the same one. Radioimmunoassay and Ultraviolet Spectrophotometry were performed to increased or decreased of serum myoglobin and aldolase. Serum myoglobin significantly increased in electrical stimulated groups[EST10' $(30.20{\pm}5.27ng/ml)$, EST20'$(31.65{\pm}3.96ng/ml)$, EST30'$(31.95{\pm}2.0ng/ml)$] to be compared with control group$(24.43{\pm}2.20ng/ml)$. Aldolase significantly increased in electrical stimulated groups [EST10' ($6.85{\pm}1.17$ Sigma U/mL), EST20'($6.70{\pm}1.46$ Sigma U/mL), EST30'($6.56{\pm}1.01$ Sigma U/mL)) to be compared with control group($5.03{\pm}1.86$ Sigma U/mL). The results of this study show that isotonic exercise result in electrical stimulation treatment increased serum myoglobin content and aldolase activity. In conclusion, our results support that stimulation release serum myoglobin and increase aldolase activity.

어육의 배소에 의한 지질산화에 관한 연구 III. 적색육어의 배소 및 재가열에 의한 heme 화합물의 변화

조호성,이강호,주동식,김경업,임상선,이종호,CHO Ho-Sung,LEE Kang-Ho,JOO Dong-Sik,KIM Gyeong-Eup,LIM Sang-Sun,LEE Jong-Ho 한국수산과학회 1998 한국수산과학회지 Vol.31 No.4

고등어와 꽁치를 온도와 시간을 달리하여 배소한 것과 배소한 시료를 저온 ($5\pm2^{\circ}C$)에서 저장한 후 단계적으로 가열처리했을 때 발생하는 지질산화에 heme 화합물이 미치는 영향을 알아보았다. Heme 화합물 중 myoglobin 함량은 고등어나 꽁치모두 $180^{\circ}C$에서 20분간 어피 제거육의 상태로 배소한 것이 가장 감소폭이 컸으며 가열. 재가열에 의해서도 지속적으로 감소하였다. 특히 $180^{\circ}C$에서 어피 제거육의 상태로 20분간 배소한 것은 생시료에 비해 myoglobin 함량이 약 $62\%$ 정도 감소하였다 Myoglobin의 감소에 의한 metmyoglobin 함량은 고등어와 꽁치 모두 점차증가하는 경향이였으며, 배소온도가 낮을수록 그리고 어피를 제거한 경우가 metmyoglobin의 생성이 가장 많았다. 총철 함량은 배소온도와 시간을 달리하여 처리하여도 두 어종 모두 생시료와는 함량 차이가 거의 없었다. 비heme 철은 고등어의 경우 총철 함량에 비해 약 $28\%$의 비heme 철이 함유되어 있었으며. $180^{\circ}C$에서 20분간 어피 제거육의 상태로 배소했을 경우 생시료에 비해 약 $96\%$ 정도 비heme 철 함량이 증가하였다. 꽁치는 생시료에는 건물당으로 약 1.6mg/100g 함유되어 있었으며, $220^{\circ}C$에서 10분 배소시에는 총철 함량에 대해 약$40\%$. $200^{\circ}C$에서 15분 배소시에는 $41\~43\%$ 그리고 $180^{\circ}C$에서 20분 배소시에는 약 $48\%$의 비heme 철이 생성되었다. Heme 철 함량은 고등어와 꽁치 모두 비heme철의 증가에 반비례해서 감소하는 경향이였으며 또한 배소시간이 길수록 그리고 어피 잔존육보다는 어피 제거육의 감소폭이 더 컸다. In order to elucidate the effect of heme compounds on lipid oxidation in roasted and/or reheated mackerel and pacific saury, changes in myoglobin, metmyoglobin, total iron, nonheme iron and heme iron content were measured. Throughout the steps of roasting, heating and reheating, myoglobin content was decteased more rapidly roasted at $180^{\circ}C$ for 20 min in fillet samples than those roasted at $200^{\circ}C$ for 15 min or $220^{\circ}C$ for 10 min. When the skinless samples were roasted at $180^{\circ}C$ for 20 min, about $38\%$ of myoglobin in raw meat were remained. The skinless fillet roasted at the lower temperature resulted the higher level of metmyoglobin due to the reduced myoglobin. Regardless of roasted temperature and time, total iron content was retained the level of raw meat throughout processing. 2 times of nonheme iron content was noted in the skinless fillet samples roasted at $180^{\circ}C$ for 20 min. All samples, heme iron content was decreased much lower by roasted temperature and in absence of skin on fillet. It was decreased about $33\%$ when roasted at $180^{\circ}C$ for 20 min in the skinless fillet in case of pacific saury.

BPM에 따른 운동강도가 스피닝 운동 시 중년여성의 CK, LDH, Myoglobin과 CRP, WBC, Fibrinogen에 미치는 영향

박설아(Park,Seul-A),김찬회(kim,Chan-Hoi),안재만(An, Jae-Man),이태복(Lee Tae-Bok) 한국웰니스학회 2018 한국웰니스학회지 Vol.13 No.3

본 연구는 BPM에 따른 운동강도가 스피닝(Spinning)운동 시 중년 여성의 CK, LDH, Myoglobin(근육손상)과 CRP, WBC, Fibrinogen(염증인자)의 변화 및 근육 회복을 확인하기 위하여 40~50대 중년여성 14명을 대상으로 중강도 운동그룹 7명, 고강도 운동 그룹 7명으로 나누어 무선 할당 배정방식으로 실시하였다. 피험자들은 운동 전 30분간 휴식을 취한 후 1차 채혈을 실시, 스피닝 중강도(60HRmax, 150bpm)와 고강도(75%HRmax, 160bpm)운동 프로그램으로 각각 실시한 뒤 2차 채혈을 실시하였으며, 30분간 휴식기를 갖고 3차 채혈을 실시하여 다음과 같은 결과를 도출하였다. CK, LDH, CRP변화는 유의한 차이가 나타나지 않았으나, Myoglobin변화는 시기에서 전〈후 유의한 차이(p〈.05)가 나타났다. WBC, Fibrinogen 변화는 집단에서 유의한 차이(p〈.05)가 나타났으며, 집단 간 차이검증 결과 유의한 차이(p〈.05)가 나타났다. 결론적으로 유의한 차이는 나타내었으나,두 집단의 운동 전, 운동 직후, 휴식 후 CK, LDH, Myoglobin과 CRP, WBC, Fibrinogen 변화에서 기준범위를 벗어나지 않아 중강도(60HRmax, 150bpm)와 고강도(75%HRmax, 160bpm)가 영향을 미치지 않는 것으로 적절한 강도로 사료된다. This study examined the effect of exercise strength in spinning exercise according to BPM on the changes of CK, LDH, Myoglobin (muscle damage) and CRP, WBC, Fibrinogen (inflammatory factors) and muscle recovery. To analyze the effect, this study used 14 women in their 40s and 50s, dividing them into 2 groups : the mid-strength group(n=7), high-strength group(n=7). They were randomly allocated. Those women were under blood-gathering three times : the first one was done after 30 minute rest before spinning exercise ; the second one was done after finishing mid-strength exercise(60HRmax, 150bpm) and high-strength exercise (75%HRmax, 160bpm) ; the third one after 30 minute rest. The findings of the tests are as follows. While there were no significant differences in changes in CK, LDH, and CRP, there was significant differences in Myoglobin before and after exercise (p<.05). There was significant differences between groups in changes of WBC, Fibrinogen (p<.05). The test of differences between groups showed significant differences (p<.05). In conclusion, the changes in muscle damages and inflammatory factors do not exceed the standard values for two groups before exercise, immediately after exercise, and after rest. So, it was found that mid-strength exercise (60HRmax, 150bpm) and high-strength exercise (75%HRmax, 160bpm) have no effect on CK, LDH, Myoglobin and CRP, WBC, Fibrinogen.

Immunohistochemical Study of Myogenic Sarcomas

Lee, Kyo Young,Kang, Chang Suck,Kim, Won Il,Shim, Sang In,Kim, Sun Moo CATHOLIC MEDICAL CENTER 1988 Bulletin of the Clinical Research Institute Vol.16/17 No.1

Using immunohistochemical staining with monoclonal antibodies to muscle cell components, histogenetical grouping and differential diagnosis of myogenic sarcoma could be possible. However immunohistochemical staining properties of myogenic sarcomas are not well defined in the formalin-fixed, paraffin-embedded tissue. We attempted to differentiate leiomyosarcoma from rhabdomyosarcoma. Twenty-nine formlin fixed myogenic sarcomas were investigated by means of immunohistochemical technique with the use of monoclonal antibodies panel against vimentin, desmin and myoglobin. In addition, the degree of differentiation of these sarcomas was investigated in connection with the immunoreactivity and intensity of immunohistochemical stains. The results were as follows; 1. Vimentin was detected in nine (60%) out of fifteen cases of leiomyosarcoma and in seven (50%) out of fourteen cases of rhabdomyosarcoma. 2. Desmin was detected in seven (47%) out of fifteen cases of leiomyosarcoma and in eight (57%) out of fourteen cases of rhabdomyosarcoma. 3. Myoglobin was detected in eleven (79%) out of fourteen cases of rhabdomyosarcoma. In all leiornyosarcomas, myoglobin was not detected. 4. The immunoreactivity and intensity of these sarcomas with anti-desmin and anti-myoglobin were related to the degree of tumor cell differentiation, but vimentin was more frequently detected in the poorly differentiated tumor cells. The above results suggest that demonstration of vimentin, desmin and myoglobin in the formalin-fixed, parnffin-embedded tissue may be a helpful adjunct tool in the diagiosis of myogenic sarcoma.

Purification and characterization of Fab fragments with rapid reaction kinetics against myoglobin.

Song, Hyung-Nam,Kim, Dong-Hyung,Park, Sung-Goo,Lee, Myung Kyu,Paek, Se-Hwan,Woo, Eui-Jeon Japan Society for Bioscience, Biotechnology, and A 2015 Bioscience, Biotechnology, and Biochemistry Vol.79 No.5

<P>Myoglobin is an early biomarker for acute myocardial infarction. Recently, we isolated the antibody IgG-Myo2-7ds, which exhibits unique rapid reaction kinetics toward human myoglobin antigen. Antibodies with rapid dissociation kinetics are thought to be premature IgG forms that are produced during the early stage of in vivo immunization. In the present study, we identified the epitope region of the IgG-Myo2-7ds antibody to be the C-terminal region of myoglobin, which corresponds to 144-154 aa. The Fab fragment was directly purified by papain cleavage and protein G affinity chromatography and demonstrated kinetics of an association constant of 4.02 10(4) M(-1) s(-1) and a dissociation constant of 2.28 10(-2) s(-1), which retained the unique reaction kinetics of intact IgG-Myo2-7ds antibodies. Because a rapid dissociation antibody can be utilized for antibody recycling, the results from this study would provide a platform for the development of antibody engineering in potential diagnostic areas such as a continuous monitoring system for heart disease.</P>

Myoglobin: A Promising Exogenous Reference Marker Using in Proteomics Analysis

Tao Wang,이홍구,황진희,오진주,임지나,강한석,주종길,이규섭 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.2

In 2-dimensional gel electrophoresis (2-DE)analysis, quantification of protein expression difference is difficult because of the high variation of spot sizes among gels. Therefore, identification of a reference marker for data normalization is necessary. In this study, consistent spot size and high reproducibility of myoglobin in 2-DE gels were successfully achieved. The use of myoglobin not only improved quantification of protein expression differences in images from different samples of the same tissue, but also made the comparison of difference in protein expression among different tissues possible. Thus, myoglobin is promising as a reference marker for data normalization in proteomic analysis.