찜질 훈련에 따른 인체의 내열성과 내한성과의 관련성

박주희(Joo-Hee Park),황수경(Soo-Kyung Hwang),최정화(Jeong-Wha Choi) 한국생활환경학회 2010 한국생활환경학회지 Vol.17 No.4

The purpose of this study is to analyze the relationships between heat tolerance and cold tolerance resulting from continuous exposure to various jjimjilbang. 17 male subjects were participated for 26-weeks-jjimjil training and heat/cold tolerance test. Through the cluster analysis, we studied the changes of heat/cold tolerance level based on physiological values; T<SUB>re</SUB>, △T<SUB>re</SUB>, T<SUB>sk</SUB> △T<SUB>sk</SUB>, TBWL, REE. Then the tolerance level of each subjects were examined to analyze the main effect (improved heat tolerance) and side effect (reduced cold tolerance) of jjimjil training. According to jjimjil training, the heat tolerance tends to improved (n=13) in rectal temperature, skin temperature and sweat rate (total body weight loss) especially in fire sudatorium-jjimjil group and lounge-rest group. And the cold tolerance tends to improved (n=12) in rectal temperature, skin temperature and resting energy expenditure (resting metaboilc rate) especially in ice room-rest group. So, we identified that according to proper jjimjil training, heat tolerance can be improved without reduced their own cold tolerance. It means jjimjil training in this study is generally enough stimulation for promoting heat tolerance, and jjimjil training method including intensity of temperature stimulation, exposure time, resting method and seasonal effect should be considered for proper jjimjil training recommendations.

자연과학편 : 30분 중강도 트레드밀 운동을 적용한 열 적응 훈련이 운동내열성에 미치는 영향

박계순(KyeSoonPark),김경배(KyoungBaeKim),전태원(TaeWoonJun) 한국체육학회 2006 한국체육학회지 Vol.45 No.6

본 연구의 목적은 30분 중강도 트레드밀 운동을 적용한 열 적응 훈련이 운동내열성에 어떠한 영향을 미치는가를 알아보는 것이다. 연구대상은 건강한 남자 대학생 37명이었으며, 3가지 열 적응 훈련 집단으로 배정하였다: Exercise+Heat, Heat, Control. Exercise+Heat 집단은 14일간 매일 30분간 35 ℃, 70% RH 조건 하에서 최대산소섭취량의 60%(전반부 7일)와 75%(후반부 7일) 강도로 트레드밀 운동을 실시하였으며, Heat 집단은 운동 없이 동일한 조건에서 열 적응만을 하였다. 운동내열성은 35 ℃, 70% RH 조건 하에서 5 km/h 속도로 1시간 동안 트레드밀 걷기 운동을 실시할 때, 직장온, 피부온, 심박수, 총 발한량, 국소 발한량, 발한개시시간, 땀 Na⁺ 농도, 생리적 피로도 등을 통해 검사하였다. 연구 결과, 총 발한량 이외에는 열 적응 훈련의 효과가 나타나지 않았다. 열 적응 효과를 얻기 위해서는 환경기후(온도, 습도, 계절)와 운동(강도, 기간, 시간) 조건을 적절히 조합하여야 할 것이다. The purpose of this study was to investigate the effects of heat acclimation exercising in 30 minutes, moderate intensity, and treadmill on exercise heat tolerance. The subjects, 37 healthy university male students, were assigned to three groups of heat acclimation: Exercise+Heat, Heat, and Control. The Exercise+Heat group was acclimated over 14 consecutive days through 30 min/day treadmill walking (35 ℃, 70% relative humidity (RH)) and the Heat group was acclimated at same conditions without exercise. The rectal and skin temperature, heart rate, total and local sweat rates, onset time of sweating, sodium concentration of sweat, and physiological strain index (PSI) were measured in a climatic chamber at 35 ℃, 70% RH, with treadmill walking (5 km/h, 1 hr) for the analysis of exercise heat tolerance. As the results, the interaction between the heat acclimation type and time was not shown the effect on exercise heat tolerance, but there was a significant tendency in the total sweat rate. These results suggest that the environment climates (temperature, humidity and season) and the exercise modes (intensity, duration and time) should match each other to have any effect of heat acclimation.

Use of Heat Stress Responsive Gene Expression Levels for Early Selection of Heat Tolerant Cabbage ( <i>Brassica oleracea</i> L.)

Park, Hyun Ji,Jung, Won Yong,Lee, Sang Sook,Song, Jun Ho,Kwon, Suk-Yoon,Kim, HyeRan,Kim, ChulWook,Ahn, Jun Cheul,Cho, Hye Sun Molecular Diversity Preservation International (MD 2013 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.14 No.6

<P>Cabbage is a relatively robust vegetable at low temperatures. However, at high temperatures, cabbage has disadvantages, such as reduced disease tolerance and lower yields. Thus, selection of heat-tolerant cabbage is an important goal in cabbage breeding. Easier or faster selection of superior varieties of cabbage, which are tolerant to heat and disease and have improved taste and quality, can be achieved with molecular and biological methods. We compared heat-responsive gene expression between a heat-tolerant cabbage line (HTCL), “HO”, and a heat-sensitive cabbage line (HSCL), “JK”, by Genechip assay. Expression levels of specific heat stress-related genes were increased in response to high-temperature stress, according to Genechip assays. We performed quantitative RT-PCR (qRT-PCR) to compare expression levels of these heat stress-related genes in four HTCLs and four HSCLs. Transcript levels for heat shock protein <I>BoHsp70</I> and transcription factor <I>BoGRAS</I> (<I>SCL13)</I> were more strongly expressed only in all HTCLs compared to all HSCLs, showing much lower level expressions at the young plant stage under heat stress (HS). Thus, we suggest that expression levels of these genes may be early selection markers for HTCLs in cabbage breeding. In addition, several genes that are involved in the secondary metabolite pathway were differentially regulated in HTCL and HSCL exposed to heat stress.</P>

폭염 대응을 위한 고령 남녀의 여름철 행동성 체온조절 반응과자각적 내열성 비교 분석

박준희,이주영 한국생활환경학회 2018 한국생활환경학회지 Vol.25 No.6

This study was conducted to investigate behavioral thermoregulatory responses and self-identified thermal tolerance in summer for elderly males and females. A total of 259 older people participated in this survey: 121 males (76.2±6.7 yr in age) and 138 females (79.2±6.7 yr in age). A questionnaire with 29 questions was used and the questions consisted of self-identified heat and cold tolerances, wearing habits, lifestyles related to the heat, and demographic characteristics. There was no difference in the number of clothes outdoor between older males (5.3±0.9 items) and females (5.5±1.0 items) but older females wore higher number of clothes at home and during sleep compared to older males (P<0.01). Older males preferred to wear clothes cooler than older females when they are inside an airconditioned building or vehicle (P<0.05). Respondents with vulnerable self-identified heat tolerance were 3.4 times higher than those with invulnerable self-identified heat tolerance while no difference was found between vulnerable and invulnerable self-identified cold tolerances. Only females had correlations between self-identified heat tolerance and wearing habit preferences related to thermal sensation for going out (r=0.343), at home (r=0.334) and during sleep (r=0.328) (P<0.01). Anthropometric variables had no correlations with self-identified heat tolerance. Sex difference should be considered when developing the guidelines for the elderly’s behavioral thermoregulation in summer and advising them for improving their health and heat tolerance.

Downregulation of PyHRG1, encoding a novel secretory protein in the red alga Pyropia yezoensis, enhances heat tolerance

Narae Han,Jiwoong Wi,Sungoh Im,Ka-Min Lim,Hun-Dong Lee,Won-Joong Jeong,Geun-Joong Kim,Chan Song Kim,Eun-Jeong Park,Mi Sook Hwang,최동욱 한국조류학회I 2021 ALGAE Vol.36 No.3

An increase in seawater temperature owing to global warming is expected to substantially limit the growth of marine algae, including Pyropia yezoensis, a commercially valuable red alga. To improve our knowledge of the genes involved in the acquisition of heat tolerance in P. yezoensis, transcriptomes sequences were obtained from both the wild-type SG104 P. yezoensis and heat-tolerant mutant Gy500. We selected 1,251 differentially expressed genes that were up- or downregulated in response to the heat stress condition and in the heat-tolerant mutant Gy500, based on fragment per million reads expression values. Among them, PyHRG1 was downregulated under heat stress in SG104 and expressed at a low level in Gy500. PyHRG1 encodes a secretory protein of 26.5 kDa. PyHRG1 shows no significant sequence homology with any known genes deposited in public databases to date. However, PyHRG1 homologs were found in other red algae, including other Pyropia species. When PyHRG1 was introduced into the single-cell green alga Chlamydomonas reinhardtii, transformed cells overexpressing PyHRG1 showed severely retarded growth. These results demonstrate that PyHRG1 encodes a novel red algae-specific protein and plays a role in heat tolerance in algae. The transcriptome sequences obtained in this study, which include PyHRG1, will facilitate future studies to understand the molecular mechanisms involved in heat tolerance in red algae.

Morphological, Physiological and Biochemical Responses of Gerbera Cultivars to Heat Stress

Chen, Wen,Zhu, Xiaoyun,Han, Weiqing,Wu, Zheng,Lai, Qixian Korean Society of Horticultural Science 2016 원예과학기술지 Vol.34 No.1

Heat stress is an agricultural problem for Gerbera jamesonii, and it often causes poor seedling growth, reduced flower yield and undesirable ornamental characteristics of flowers. However, little is known about the effect of heat stress on the morphological, physiological and biochemical characteristics of gerbera plants. Here, the responses of six cultivars of Gerbera jamesonii to heat stress were investigated. Under a 1-d heat treatment at $45^{\circ}C$, the leaves of gerbera cultivars showed yellowing, wilting, drying and death to varying degrees. The heat treatment also resulted in increased electrical conductivity, decreased soluble protein and chlorophyll contents, and the accumulation of malondialdehyde (MDA) and proline in leaves. Moreover, heat tolerance differed among the six tested gerbera cultivars. Our results demonstrated that among the six gerbera cultivars, 'Meihongheixin' is a heat-resistant cultivar, whereas 'Beijixing' is a heat-sensitive one. 'Shijihong' and 'Linglong' are relatively heat-resistant cultivars, and 'Dadifen' and 'Taiyangfengbao' are relatively heat sensitive.

주요 국산밀 품종과 내고온성 터키 유전자원을 이용한 내고온성 관련 SSR 마커 평가

손재한,김경훈,정영근,박종철,김경호,김양길,오영진,송태화,김보경,강천식 한국작물학회 2015 한국작물학회지 Vol.60 No.3

밀의 등숙기간, 종자 수, 종자무게와 관련된 SSR 마커14개를 이용하여 터키에서 분양받은 내고온성 유전자원23개와 국산밀 품종 7개, Chinese spring 1개 등 31개를분석한 결과, 전체 86개의 대립유전자(평균 6.14개)가 확인되었다. 평균 PIC 값은 0.64로 나타났다. 다형성 분석을통한 마커 데이터를 이용하여 계통 분석을 한 결과 크게세 그룹으로 형성되었다. 올밀이 가장 바깥 그룹으로 형성되었고 올그루, 고소, 조품 등이 터키자원과 다르게 단일그룹으로 형성되었다. 금강, 조경, 백중 등 세 품종은 터키자원들과 같은 그룹으로 나타났지만 밀접하게 연관되어있지는 않은 것으로 나타났다. 국내 품종 중 올, 올그루, 조품, 고소 등 4개와 금강, 조경, 백중 등 3개가 서로 다른그룹으로 형성되었다. 두 그룹의 차이는 파성 II와 III의 차이로 구별되었다. High temperature is one of major environmental stress. Heat tolerance managing is difficult through the phenotypic selection, so marker assistant selection (MAS) using molecular markers like as RAPD, SSR etc. was tried to select useful traits for heat tolerance. Fourteen SSR markers reported by previous research were selected for this research. We tried to evaluate 14 SSR markers for MAS using 31 useful wheat resources including 24 crossing line from Turkey, six Korean wheat cultivars and Chinese spring. The average of the number of alleles and PIC values in this study were 6.14 and 0.64, respectively. Two major clades and four sub clades were grouped by phylogenetic tree using UPGMA. Four Korean wheat cultivars were distinct from other Turkey resources in the phylogenetic dendrogram. From the results, we expected that these markers were able to adapt to screening wheat genotyping for heat tolerance. Keywords : heat tolerance, MAS, SSR, wheat

Morphological, Physiological and Biochemical Responses of Gerbera Cultivars to Heat Stress

Wen Chen,Xiaoyun Zhu,Weiqing Han,Zheng Wu,Qixian Lai 한국원예학회 2016 원예과학기술지 Vol.34 No.1

Heat stress is an agricultural problem for Gerbera jamesonii, and it often causes poor seedling growth, reduced flower yield and undesirable ornamental characteristics of flowers. However, little is known about the effect of heat stress on the morphological, physiological and biochemical characteristics of gerbera plants. Here, the responses of six cultivars of Gerbera jamesonii to heat stress were investigated. Under a 1-d heat treatment at 45°C, the leaves of gerbera cultivars showed yellowing, wilting, drying and death to varying degrees. The heat treatment also resulted in increased electrical conductivity, decreased soluble protein and chlorophyll contents, and the accumulation of malondialdehyde (MDA) and proline in leaves. Moreover, heat tolerance differed among the six tested gerbera cultivars. Our results demonstrated that among the six gerbera cultivars, ‘Meihongheixin’ is a heat-resistant cultivar, whereas ‘Beijixing’ is a heatsensitive one. ‘Shijihong’ and ‘Linglong’ are relatively heat-resistant cultivars, and ‘Dadifen’ and ‘Taiyangfengbao’ are relatively heat sensitive.

Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato

Danijel Pantelic,Ivana Č. Dragićević,Jelena Rudić,Jianming Fu,Ivana Momčilović 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.3

Potato (Solanum tuberosum L.) plants are highly vulnerable to heat stress. Even moderately elevated temperatures can disturb the process of tuberization in this important crop, causing a decline in tuber initiation, a reduction in tuber bulking, and tuber disorders. In the present study, we investigated the effects of heat stress on tuberization in two potato cultivars, the heat-sensitive cultivar Désirée and the heat-tolerant cultivar Festival, using an in vitro system. A temperature of 29 °C reduced tuber initiation and tuber bulking, and stimulated shoot elongation in cv. Désirée, while this temperature treatment did not significantly alter tuberization or shoot elongation in cv. Festival. In addition, high temperature interfered with the onset of microtuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage in both cultivars. Stress-responsive proteins HSP17.6-CI, HSP101, and eEF1A showed heat-induced accumulation patterns in shoots and microtubers of these two cultivars, with the exception of a decline in the abundance of eEF1A in cv. Désirée microtubers under heat stress. High levels of HSP17.6-CI in microtubers of cv. Désirée did not ameliorate the effects of heat stress on tuberization of this relatively heat-sensitive cultivar. Conversely, a higher level of eEF1A under heat stress in microtubers of the heat-tolerant cv. Festival indicated a possible function of this protein in alleviating the negative effects of high temperature on potato tuberization. This study suggested that analysis of stress-responsive proteins in potato microtubers combined with assessment of tuberization parameters in vitro may represent a useful screening procedure for selection of heat-tolerant potato genotypes.

Overexpression of C-Repeat Binding Factor1 (CBF1) Gene Enhances Heat Stress Tolerance in Arabidopsis

윤상대,Kim Myung-Hee,Oh Sung Aeong,소문수,박순기 한국식물학회 2022 Journal of Plant Biology Vol.65 No.3

High temperature affects plant growth, development, and seed production. We generated Arabidopsis transgenic plants overexpressing abiotic stress-tolerance associated genes (CBF1, GolS1, NDPK2, YUC6, and PRE1) to investigate the effects of these genes on heat stress (HS) tolerance. We evaluated thermotolerance by assessing the survival rate of T2 seedlings under acute HS conditions. C-repeat binding factor 1-overexpressing (CBF1-OE) plants exhibited drastically improved HS tolerance, whereas overexpression of the other examined genes yielded marginal effects. Consistent with the ability of CBF1 to regulate transcription, we found that the transcription of several heat-shock inducible genes was remarkably increased in CBF1-OE plants under HS conditions. Furthermore, the expression of several reactive oxygen species (ROS)-responsive genes related to oxidative stress was notably upregulated in CBF1-OE plants. Thus, the thermotolerance of CBF1-OE plants is at least partly associated with the elevated expression of several HS-inducible and ROS-responsive genes. Our study showed that CBF1 plays a role in increasing HS tolerance in Arabidopsis in addition to its previously established roles in cold/drought tolerance.