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신기혜,소문수,이린아,이인혜,이수민,박순기 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.1
Ethylene controls myriad aspects of plant growth through-out developmental stages in higher plants. It has been well established that ethylene-responsive growth entails extensive crosstalk with other plant hormones, particularly auxin. Here, we report a genetic mutation, named 1-aminocy-clopropane carboxylic acid (ACC) resistant root1-1 (are1-1) in Arabidopsis thaliana (L.) Heynh. The CONSTITUTIVE TRIPLE RESPONSE1 (CTR1) encodes a Raf-related protein, functioning as an upstream negative regulator of ethylene signaling in Arabidopsis thaliana. We found that the ctr1-1, a kinase-inactive allele exhibited slightly, but significantly, longer root length, compared to ACC-treated wild-type or ctr1-3, a null allele. Our genetic studies unveiled the existence of are1-1 mutation in the ctr1-1 mutant, as a second-site modifier which confers root-specific ethylene-resistan-ce. Based on well-characterized crosstalk between ethylene and auxin during ethylene-responsive root growth, we performed various physiological analyses. Whereas are1-1 displayed normal sensitivity to synthetic auxins, it showed modest resistance to an auxin transport inhibitor, 1-N-naphthylphthalamic acid. In addition, are1-1 mutant exhibited ectopically altered DR5:GUS activity upon ethylene-treatment. The results implicated the in-volvement of are1-1 in auxin-distribution, but not in auxin-biosynthesis, -up-take, or -sensitivity. In agreement, are1-1 mutant exhibited reduced gravitropic root growth and defective redistribution of DR5:GUS activity upon gravi-stimulation. Taken together with genetic and molecular analysis, our results suggest that ARE1 defines a novel locus to control ethylene-responsive root growth as well as gravitropic root growth presumably through auxin distribution in Arabidopsis thaliana.
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Overexpression of C-Repeat Binding Factor1 (CBF1) Gene Enhances Heat Stress Tolerance in Arabidopsis
윤상대,Kim Myung-Hee,Oh Sung Aeong,소문수,박순기 한국식물학회 2022 Journal of Plant Biology Vol.65 No.3
High temperature affects plant growth, development, and seed production. We generated Arabidopsis transgenic plants overexpressing abiotic stress-tolerance associated genes (CBF1, GolS1, NDPK2, YUC6, and PRE1) to investigate the effects of these genes on heat stress (HS) tolerance. We evaluated thermotolerance by assessing the survival rate of T2 seedlings under acute HS conditions. C-repeat binding factor 1-overexpressing (CBF1-OE) plants exhibited drastically improved HS tolerance, whereas overexpression of the other examined genes yielded marginal effects. Consistent with the ability of CBF1 to regulate transcription, we found that the transcription of several heat-shock inducible genes was remarkably increased in CBF1-OE plants under HS conditions. Furthermore, the expression of several reactive oxygen species (ROS)-responsive genes related to oxidative stress was notably upregulated in CBF1-OE plants. Thus, the thermotolerance of CBF1-OE plants is at least partly associated with the elevated expression of several HS-inducible and ROS-responsive genes. Our study showed that CBF1 plays a role in increasing HS tolerance in Arabidopsis in addition to its previously established roles in cold/drought tolerance.
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Expression analysis of two rice pollen-specific promoters using homologous and heterologous systems
Tien Dung Nguyen,Moe Moe Oo,문선옥,배현경,오성앵,소문수,송종태,김정회,정기홍,박순기 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.5
Tissue-specific promoters are a very useful tool for manipulating gene expression in a target tissue or organ; however, their range of applications in other plant species has not been determined, to date. In this study, we identified two late pollen-specific rice promoters (ProOsLPS10 and ProOsLPS11) via meta-anatomical expression analysis. We then investigated the expression of both promoters in transgenic rice (a homologous system) and Arabidopsis (a heterologous system) using ProOsLPS10 or ProOsLPS11::GFP-GUS constructs. As predicted by microarray data, both promoters triggered strong GUS expression during the late stages of pollen development in rice, with no GUS signals detected in the examined microspores and sporophytic tissues. Interestingly, these promoters exhibited different GUS expression patterns in Arabidopsis. While in Arabidopsis, the OsLPS10 promoter conferred GUS expression at the uniand bi-cellular pollen stages, as well as at the shoot apical region during the seedling stage, the OsLPS11 promoter was not active in the pollen at any stage, or in the examined sporophytic tissues. Furthermore, by performing a complementation analysis using a sidecar pollen (scp) mutant that displays developmental defects at the microspore stage, we found evidence that OsLPS10 promoter, which can be applied in Arabidopsis, is useful for directing gene expression in the early stages of pollen development. Our results indicate that the OsLPS10 and OsLPS11 promoters can drive the expression of target genes during the late stages of pollen development in rice, but not in Arabidopsis. Our results also emphasize the necessity of confirming the applicability of an established promoter to heterologous systems.
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