Evaluation of human papillomavirus (HPV) genotyping assays using type-specific HPV L1 reference DNA

한경호 한국유전학회 2021 Genes & Genomics Vol.43 No.7

Background Human papillomaviruses (HPV) are known to play a central etiological role in the development of cervical cancer. General HPV genotyping methods consist of PCR with consensus primers combined with various detection methods. Objective The aim was to develop HPV L1 DNA reference materials to evaluate the sensitivity, specifcity, and accuracy of genotyping results obtained from the HPV DNA Genotyping Chip (HPV CHIP) and RFMP assays. Methods In this study, the Ministry of Food and Drug Safety (MFDS) established reference DNA materials for the L1 gene from 41 subtypes of anogenital HPV to aid in genotyping human papillomavirus (HPV) strains. Of these, 22 subtypes were obtained from cervical scrape samples of Korean women and 19 subtypes were synthesized. These reference materials include 13 high-risk types (HPV-16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68), 3 probable high-risk types (HPV-26, 53, and 66), 16 low-risk types (HPV-6, 10, 11, 27, 34, 40, 42, 43, 44, 54, 55, 61, 70, 72, 73, and 81), and 8 undetermined-risk types (HPV-3, 57, 62, 67, 69, 71, 74, and 84). After confrming the sequences by standard methods, these HPV L1 DNA reference materials were then used to compare results from the HPV DNA Genotyping Chip (HPV CHIP) and restriction fragment mass polymorphism (RFMP) assays. Results Data collected from the HPV CHIP and RFMP assay showed comparably high sensitivity and accuracy. Both assays could detect 102 or more copies/μl of HPV L1 DNA from 39 types of HPV, with higher accuracy in detecting samples with mixed types of HPV. Conclusion The present study confrms the HPV L1 DNA reference materials developed by MFDS are reliable and useful for the evaluation of HPV genotyping assays.

한국 여성 자궁경부 종양 환자에서 인유두종 바이러스 유전자형 분석

박태철,김찬주,이근호,윤주희,김지훈,고영미,남궁성은,박종섭 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.1

인유두종 바이 러스(human papillomavirus; HPV)는 자궁경부암의 발생 에 있어서 가장 중요한 원인으로 인정되고 있으며, 자궁경부 세포진 검사에 대해 보조적인 방법으로 HPV 진단법의 효용성이 확인되었다. HPV 백신의 개발이 예상되고 있는 시점에서 한국 여성에서 HPV 유전자형 분포도에 대한 지식은 자궁경부암의 진단과 치료와 예방에 있어서 매우 중요할 것으로 생각된다. 2001년 11월부터 2002년 5월까지 가톨릭대학교 의과대학 강남 성모 병원 산부인과를 방문한 외래 및 입원 환자를 대상으로 하여, 자궁경부 세포진 또는 병리 조직 검사 소견에 따른 HPV 유전자형의 분포를 분석하였다. 환자들의 평균 연령은 43.4세이며, 21세에서 91세의 범위에 있었다. 자궁경부 세포진 또는 병리 조직 검사 소견에 따라 정상 세포진 소견을 보이는 여성(n=290), LSIL (low-grade squamous intraepithelial lesion) 세포진 검사와 일치하는 조직 검사 결과로 HPV 감염 또는 경증 상피이형증으로 나타난 환자(n=68), HSIL (high-grade squamousintraepithelial lesion) 세포진 검사와 일치하는 조직 검사 결과로 중등도 상피이형증, 중증 상피이형증 또는 상피내암으로 나타난 환자(n=51), 자궁경부암 환자(n=55)로 분류하였으며, HPV DNA chip을 이용하여 각 군에서 채취한 자궁경부 세포에서 HPV의 존재를 파악하고 유전자형 분포와 복합 감염을 관찰하였다. 각 군당 HPV 발견율은 정상군 290명 중 51명(17.6%)에서 HPV 양성이었으며, LSIL에서 68명 중 50명(73.5%),HSIL에서 51명 중 47명(92.2%),자궁경부암에서 62명 중 59명(95.1%)에서 HPV 양성이었으며, 그 중 편평세포암환자 55명 중 53명(96.4%)에서, 선암 환자 7명 중 6명(85.7%)에서 HPV 양성 소견이 관찰되었다 HPV-16형은 정상군에서 7.9% (23/290), LSIL에서 24.2% (16/68), HSIL에서 33.3% (17/51), 자궁경부암에서 75.8% (47/62) 검출되었으며, 자궁경부 병소의 중증도가 진행됨에 따라 HPV-16형의 발견 빈도가 유의하게 증가됨을 관찰할 수 있었다(β<0.01). 그 외 HPV-18, -58형들은 자궁경부암 환자와 HSIL에서 높게 검출되었으며, HPV-18, -51, -52, -58, -68형들은 LSIL에서 높게 검출되었다(β<0.05). 여러 종류의 HPV들이 검출되는 복합 감염의 경우는 정상군에서 19명(37.3%), LSIL에서 21명(42.0%), HSIL에서 13명(27.7%), 자궁경부암에서 17명(28.8%)에서 바이 러스가 발견되 었다한국 여성의 자궁경부 정상 세포진에서 HPV 감염, 자궁경부 상피이형증을 거쳐서 자궁경부암으로 진행되는 상태에 따라 HPV 감염률의 차이와 HPV 유전자형의 분포도 및 복합 감염 여부를 파악할 수 있었다. 자궁경부병소를 가진 여성에서 HPV를 탐색한 본 연구 결과로서 HPV감염과 자궁경부 상피 이형증의 예후를 결정할 수있는 분자 생물학적 지표로 사용하거나 자궁경부 상피내 암과 자궁경부암을 조기 진단하기 위한 보조적 인 방법으로 가능성을 고려할 수 있을 것으로 생각되며, 자궁경부암의 예방을 위하여 향후 개발될 HPV 백신을 사용하기위한 한국 여성에서의 기초 자료로서 가치가 있을 것으로 사료된다. Objective : It has been widely acknowledged that human papillomavirus (HPV) is the most important cause of cervical carcinoma, and the HPV DNA test has been proved as an effective adjuvant to Pap smear. As the development of HPV vaccine is now at the planning stage, the role of HPV genotype profile has become very important in early diagnosis, management and prevention of cervical neoplasia. Methods : Analysis was made on the HPV genotype profile of a number of the inpatients and outpatients who visited at the Department of Gynecology, Kangnam St. Mary’s Hospital between November, 2001 and May, 2002, based on the Pap smear test and tissue pathology. The age range of the patients covered in this study was between 21 years and 91 years and the average age of the patients was 43.4 years. Based on the results of the Pap smear and tissue pathology, the patients were broken down into the following 4 groups; the women with normal cytology (n=290), the patients with LSIL (low-grade squamous intraepithelial lesion); koilocytosis and mild dysplasia (n= 68), the patients with HSIL (highgrade squamous intraepithelial lesion); moderate and severe dysplasia, or carcinoma in situ (n= 51), and the patients with cervical cancer (n=55), respectively and a study was made on the HPV genotype profile of cervix of each of the above 4 groups by utilizing HPV DNA chip. Results : The proportions of the detected HPV-16 in the individual groups were 7.9% (23/290) for women with normal cytology, 24.2% (16/68) for the patients with LSIL, 33.3% (17/51) for HSIL, 75.8% (47/62) for those with cervical cancer, respectively and it was noticed that detection rates increased significantly in accordance with advancing the severity of cervical lesions (P<0.01). Of the rest of the HPV types, HPV -18, -33, -35, -39, -52, -56, -58 were related with cervical cancer (P<0.05); HPV-18, -58 were related with HSIL (P<0.05); HPV -18, -39, -51, -52, -58, -68 were related LSIL (P<0.05). In the case of multiple HPV infections, 19 (37.3%) were detected in women with normal cytology, 21 (42.0%) were detected in patients with LSIL, 13 (27.7%) were detected in HSIL, and 17 (28.8%) were detected in those with cervical cancer, respectively, but there was no statistically significant differences. Conclusion : As a result of this study, HPV genotype profiles and multiple infections by pathological severity can be obtained for the Korean female patients groups ranging from normal cervical cytology through cervical intraepithelial neoplasia to cervical carcinoma. It is expected that the results of this study might be utilized as a molecular biological index to determine prognosis of HPV infection and cervical dysplasia or as an adjunctive diagnostic method for early detection of carcinoma in situ and cervical carcinoma, and that the results can also become very valuable basic data that can be used in developing HPV vaccine for prevention of cervical carcinoma in the near future.

Genotype Distribution of Human Papillomavirus in Women with Abnormal Cervical Cytology in an Esophageal Carcinoma High Incidence Area of China

Mai, Rui-Qin,Huang, Bo,Shen, Ling,Zhang, Guo-Hong,Hong, Liang-Li,Cai, Ying-Mu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Infection with human papillomavirus (HPV) could affect genesis of both cervical and esophageal cancers. The type-specific distribution of HPV in cervical cytology abnormalities of women has remained unclear in Shantou, an esophageal cancer high-incidence area of China. Data from 22,617 women who were subjected to cervical HPV DNA testing with simultaneous cervical cytological examination during 2009-2013 were therefore here retrospectively evaluated in a hospital-based study. Overall, 16.2% (3,584/22,114)of women with normal cytology were HR-HPV positive, with HPV-52 (4.07%) as the most common type followed by -16 (3.63%), and -58 (2.46%). Prevalence of HR-HPV was 50.3% (253/503) in women with cervical cytological abnormalities, of which in ASC-H 71.4%, ASC-US 39.1%, HSIL 80.3% and LSIL 73.7%. HPV-58 (14.12%) was the most common type for all cervical cytological abnormalities, followed by HPV-16 (13.72%), and -52 (12.72%), while the more common HPV-16 type in ASC-H (42.9%) and HSIL (36.1%), HPV-52 and -58 were the most common types for ASC-US (10.3%) and LSIL (25%), respectively. Multiple HPV co-infections were identified in 33.2% (84/253) cytology abnormalities with positive HR-HPV, and the highest prevalence of HPV-58/16 combination in HSIL (28.6%, 6/21) was observed. Our data indicated a relative high prevalence of HPV-58 and -52 in women with cervical cytological abnormalities, which should be considered in the development of next-generation vaccines for Shantou.

HPV Detection and Genotyping in Vulvar Squamous Cell Carcinoma in Northern Thailand

Siriaunkgul, Sumalee,Settakorn, Jongkolnee,Sukpan, Kornkanok,Srisomboon, Jatupol,Utaipat, Utaiwan,Lekawanvijit, Suree,Khunamornpong, Surapan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Background: The study was aimed to evaluate the prevalence and genotype distribution of HPV infection in vulvar squamous cell carcinoma (SCC) in northern Thailand and the clinicopathological difference with regard to HPV infection status. Materials and Methods: Formalin-fixed paraffin-embedded tissue samples of vulvar SCC diagnosed between January 2006 and December 2012 were collected. HPV infection was detected by nested polymerase chain reaction (PCR) with primers MY09/11 and GP5+/6+. HPV genotyping was performed using the Linear Array Genotyping Test, followed by type-specific PCR targeting the E6/E7 region of HPV16/18/52 if the Linear Array test was negative. The histologic slides of vulvar lesions and the medical records were reviewed. Results: There were 47 cases of vulvar SCC included in the study (mean patient age $57.9{\pm}13.2$ years). HPV infection was detected in 29 cases (62%), all of which had single HPV infections. HPV16 accounted for 23 (49%). The patients with HPV-positive SCC had a significantly younger mean age than those with HPV-negative tumors (52.7 years vs 66.2 years, p<0.001). There was no significant difference in tumor stage distribution with regard to the status of HPV infection. The presence of vulvar intraepithelial neoplasia (VIN) of usual type (basaloid or warty) was significantly more frequent in HPV-positive cases compared with HPV-negative cases (62% vs 6%, p<0.001), whereas differentiated-type VIN was more common in HPV-negative cases (24% vs 0%, p=0.019). Conclusions: HPV infection was detected in 62% of vulvar SCC in northern Thailand. HPV16 was the predominant genotype similar to the data reported from other regions. HPV-positive SCC occurred in younger patients compared with HPV-negative SCC, and was associated with usual-type VIN. Vaccination against HPV16/18 may potentially prevent almost one half of vulvar SCC in northern Thailand.

한국 여성 자궁경부 조직에서 분리된 인유두종 바이러스 16형의 상류전사 조절 부위 유전자 서열 변이 분석

전혜원(HW Jeon),이효표(HP Lee) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.4

It is widely accepted that human papillomaviruses (HPV), especially type 16 and type 18 are the primary causal agent for the development of invasive cervical cancer and its precursor lesions. Although the mechanisms by which HPV acts to transform cells are still not completely understood, complex interactions between virus and host cell seem to determine the biologic course of cervical cancer. In Korea, 50∼60% of the patients with cervical cancer have been associated with HPV 16. Recently phylogenetically distinct HPV 16 variants that differ from the prototype HPV 16 sequence have been identified and the significance of them in carcinogenesis is being studied. The upstream regulatory region (URR) does not encode for proteins, but it contains a complex array of overlapping binding sites for many different transcriptional factors and their genomic sequences usually diverge more than coding regions. We were analyzed to detect HPV 16 URR enhancer region variants in Korean women and to determine whether risk of cancerous lesion was associated with HPV 16 variants. Cervical tissues were taken from the women with cervical cancerous (N=125) and normal cervical biopsies (N=307). Of the HPV 16 infections detected in 70 cervical cancerous tissues and 43 normal cervical tissues, 56.0% and 14.0%. We amplified by nested-PCR and sequenced a 364-bp noncoding segment of the HPV 16 genome (nucleotide 7458∼7841). By direct sequencing of PCR product, a total of 11 point variations that differed from prototype HPV 16 sequence was detected in various combinations, giving rise to 8 distinct types of variants. Of the HPV 16 URR variants detected in 68 cervical cancerous tissues and 39 normal cervical tissues, 97.1% and 90.7%. In addition, four transcriptional factors binding sites (TEF-1, 2; YY1, 2) were found to be mutated; nucleotide 7797 variation was found in only cervical cancerous tissues. Variants of an YY1 binding site in cervical cancerous tissues were correlated to depth of invasion not to age, stage, tumor size, histologic type, and nodal metastasis. Variants of a TEF-1 binding site were not show any difference in the clinicopathologic factors. These results suggest that the URR variant is not a mutation that occurs during disease progression in susceptible individuals but is a distinct virus isolate of HPV type 16. The association with cervical cancer suggests that either the mutation may also influence the oncogenic properties of the HPV type 16 variant or that the base change is a marker of other mutations within the virus genome that may increase its oncogenicity. To trace the transmission route and natural history of HPV-associated disease, more than one gene segment` strategy using a highly variable one as a genetic marker will be necessary.

Genotype Frequency of Human Papillomavirus Determined by PCR and DNA Sequencing in Korean Women

Lee, Kyung-Ok,Seong, Hye-Soon,Chung, Soo-Jin,Jung, Na-Young,Lee, Hye-Jung,Kim, Kyung-Tae Korean Society for Clinical Laboratory Science 2006 대한임상검사과학회지(KJCLS) Vol.38 No.2

파필로마바이러스(Human papilloma virus; HPV)는 자궁경부암의 주요한 원인균으로 30종 이상의 여성성기감염과 관련된 유전자형이 보고되었으며 자궁경부암과 관련성이 높은 고위험군과 관련성이 낮은 저위험군으로 나뉘어 진다. 최근 HPV 유전자형의 임상적 활용이 높아짐에 따라 신속하고 정확하게 HPV 유전자형을 선별할 수 있는 방법이 요구되고 있다. 본 연구의 목적은 여러 가지 분자생물학적 방법 중에서 정확도가 높은 DNA 염기서열분석을 이용하여 한국인 여성에서 HPV의 유전자형분포와 빈도를 구하고자 하였다. 전국 각 지역의 3,978명으로부터 채취한 자궁경부 검체에서 DNA를 추출하고, HPV L1 유전자 영역에서 PCR을 실시하였다. PCR 양성이 나온 경우 DNA 염기서열분석을 실시하였으며 GenBank BLAST program을 이용하여 HPV 유전자형을 분석하였다. 검사대상의 평균 년령은 37.6세였으며 년령 범위는 20-73세였고, 30대 여성이 검사를 가장 많이 실시하였다(42.2%). 총 3.978명 중에서 1,174명(1,174/3,978, 29.5%)이 HPV 양성을 보였으며 136명(11.6%)이 중복감염을 보여, 총 1,310개의 HPV 유전자를 분석하였다. 본 연구에서는 21종의 고위험군, 16종의 저위험군을 포함하여 총 37종의 HPV 유전자형이 검출되었으며, HPV 고위험군의 빈도는 69.8%(914/1,310), 저위험군은 26.0% (340/1,310)로 나타났다. 년령은 20대에서 HPV 양성률이 가장 낮았으며(69.5%), 60대 이상의 검체에서 발견된 HPV는 대부분이 고위험군이었다. 고위험군에서는 HPV 16형이 13.21%로 가장 높게 나타났으며, HPV 53형이 9.62%, 58형이 9.24%로 높게 나타났다. 다음으로 HPV 70(5.50%), 33(4.73%), 66(4.20%), 18(4.05%), 52 (4.05%), 31(3.97%), 56(3.51%)의 순으로 나타났다. 저위험군에서는 HPV 62(4.20%), 61(3.89%), 6(3.59%), 81(3.59%), 84(3.51%), 11(2.6%)의 순으로 검출되었다. DNA 염기서열분석을 이용한 한국인 여성의 HPV 유전자형빈도 분석 결과는 HPV의 역학적 연구와 백신개발을 위한 자료로 유용할 것이며, 자궁경부암의 치료와 관련한 특이적 HPV 유전자형 관련 연구에 도움을 줄 것으로 사료된다. Human Papilloma viruses (HPVs) are etiological agents for cervical cancer and are classified into low- and high-risk categories. The aim of this study was to determine the frequency of the HPV genotype in the HPV screening test of Korean women using PCR-direct sequencing. Consensus primers of L1 legion were used for the amplification of HPV DNA and the PCR products (450 bps) obtained were analyzed by automatic sequencing. Sequences were compared with those in GenBank by using the BLAST program. Cervical swab samples of 3,978 women (20-73 years) were tested and the average age was 37.6 years. In this study, 1,174 samples were HPV positive out of 3,978 cervical swab samples screened (29.5%) and 136 samples (11.6%) showed a double infection. A total of 1,310 HPV genotypes were analyzed. The HPV positive rate was the lowest in the 20 years group (69.5%) and most of the samples of the > 60 years group were found HPV positive. Among thirty seven different HPV types identified by sequencing, 21 were HPV high risk types and 16 HPV low risk types were 69.8% (914/1,310) and 26.0% (340/1,310), respectively. In HPV high-risk types, 16 (13.21%), was the most frequently found. HPV 53 (9.62%) and 58 (9.24%) were also frequently found. This group was followed by HPV types 70 (5.50%), 33 (4.73%), 66 (4.20%), 18 (4.05%), 52 (4.05%), 31 (3.97%) and 56 (3.51%) in descending order of frequency. Among HPV low-risk types, 62 (4.20%), 6 (3.59%), 81 (3.59%), 84 (3.51%), and 11 (2.6%) were frequently found. In conclusion, PCR-direct sequencing could be used for quick and reliable typing of known and novel HPVs from clinical specimens. This data could be useful for epidemiological study of HPV and it also allows type-specific follow-up of women who have been treated for cervical intraepithelial neoplasia.

남·녀 대학생의 인유두종 바이러스(HPV), HPV백신의 지식과 성 전파성질환 감염예방행위의도

백선숙,송미승,전미순 한국자료분석학회 2012 Journal of the Korean Data Analysis Society Vol.14 No.6

Purpose: This study was conducted to verify the knowledge of HPV, HPV vaccine and intention for STD prevention and to find out the relationship of them among undergraduate students, both male and female. Method: The data was collected from 274 undergraduate students from May to June 2011. The data collection was done by structural survey in the form of questionnaire. Result: The average score of knowledge of HPV and HPV vaccine was 1.98 and 2.13 out of 3 respectively. There was no significant difference between the level of knowledge of HPV and HPV vaccine among them. The level of the knowledge of HPV was low, while the intention for STD prevention was high. There was a significant difference between the level of knowledge of HPV vaccine and the intention for STD prevention the students. Conclusions: Therefore we should develop the program of sexual education in order to prevent the STDs infection for the students. 본 연구는 남·녀 대학생의 인유두종 바이러스(HPV)와 HPV백신의 지식, 그리고 성 전파성질환 감염 예방 행위 의도와의 관계를 확인하기 위하여 시도되었다. 자료수집은 C시와 Y시에 있는 각 대학교의 학생 274명을 대상으로 구조화된 설문지를 이용하였으며 2011년 5월에서 6월까지 실시되었다. 자료는 SPSS Win 19.0 프로그램을 이용하여 t-검정(t-test)과 일원분산분석(one way ANOVA), Scheffe 방법, 상관관계 분석을 사용하였다. 분석결과 남녀대학생의 HPV 지식은 평균보다 약간 높았으나, 성 전파성 질환 예방의도는 매우 높았다. 대학생들의 HPV 백신의 지식 정도 및 성 전파성 질환 예방 의도에서 여학생이 남학생보다 HPV 백신지식과 성전파성질환 예방의도가 더 높았다. 또한 남녀 대학생의 HPV 백신 지식이 높으면 성 전파성 질환 예방의도가 높은 것으로 확인되었다, 따라서 대학생의 성전파성 질환을 예방하기 위한 남녀 대학생 모두를 대상으로 한 성교육 프로그램 개발과 교육이 요구된다.

사람유두종바이러스의 검출과 유전자형 분석을 위한 HPV28 Detection 검사와 HPV DNA Chip 검사의 비교 평가

신은심,배효진,송완근,정선경,황유성 대한진단검사의학회 2013 Laboratory Medicine Online Vol.3 No.4

Background: The HPV28 Detection test (Seegene) is a real-time polymerase chain reaction assay that is designed for testing a total of 28 human papillomavirus (HPV) genotypes and estimating the approximate HPV viral load. The aim of this study was to evaluate the clinical applicability of the HPV28 Detection test with regard to the prevalence of HPV infection and distribution of HPV genotypes by using the HPV28 Detection and HPV DNA Chip tests (Biomedlab). Methods: HPV DNA Chip and HPV28 Detection tests were performed for 500 cervical swab specimens. HPV genotype results were confirmed by sequencing analysis of the specimens that showed discordant results in the 2 test methods. Results: The positive rate of HPV detection determined by using HPV28 Detection and HPV DNA Chip tests were 43.8% and 40.6%, respectively. The sequencing results in 64 discordant specimens that showed single HPV infection in the 2 test methods were in complete agreement with the test results obtained with the HPV28 Detection test. The genotyping results of the HPV28 Detection test were 100% concordant in repeated experiments with HPV-infected specimens that have 12 different HPV genotypes, i.e., types 16, 31, 33, 39, 42, 51, 52, 53, 58, 66, 68, and 70. The HPV28 Detection test was 100-fold more sensitive than the HPV DNA Chip test with serially diluted HPV DNAs. Conclusions: The HPV28 Detection test can be applied in the clinical field as an HPV genotyping test can accurately identify various HPV genotypes with high specificity and low detection limit.

부산지역 유흥업소 종사 여성의 HPV 감염 및 유전자형 분포 조사

민상기(Sang-Kee Min),김성순(Sung Soon Kim),최병선(Byeong-Sun Choi),조경순(Kyung-Soon Cho),이주연(Joo-Yun Lee),김성준(Seong-Joon Kim),빈재훈(Jae-Hun Bin),박호국(Ho-Kuk Park) 한국생명과학회 2009 생명과학회지 Vol.19 No.2

부산지역의 유흥업소 종사여성을 대상으로 HPV DNA chip 시험을 통하여 HPV 유병율 및 유전자형 분포를 조사하였다. 총 660건의 자궁경부도찰 검체로부터 258건(양성율 39.1%)이 검출되었으며, 20대에서 가장 높은 유병율을 나타내었다. 양성자의 유전자형 분포는 고위험군 유전자형의 경우 HPV-16 (15.9%), -53 (10.2%), -58 (7.7%), -18 (5.2%)순이었고, 저위험군 유전자형의 경우 HPV-70 (10.4%), -6 (4.1%), -11 (2.0%)순이었다. 특이하게 고위험군 감염여성의 분포비 (62.0%)가 저위험군(14.7%) 및 고/저위험군 혼합감염(12.0%)보다 4배 가까이 높았다. 전체 양성자중 단일유형 감염자는 175건(67.8%)이고 혼합유형 감염자는 83건(32.2%)이었다. 본 연구 결과 밝혀진 부산지역 유흥업소 종사여성에서 많이 발견되는 HPV 유전자형 분포의 다양성은 국내 도입된 자궁경부암 예방백신의 효과 예측 및 국내 특성에 맞는 다가백신 개발의 기초 자료가 될 수 있을 것으로 사료된다. We tried to analyze the HPV prevalence and HPV genotypes of sexually high-risk women living in Busan, the biggest seaport of South Korea. Six hundred sixty women engaging in high-risk occupations participated in this study. The prevalence of HPV infection and HPV genotyping were determined with MyGene<SUP>®</SUP> HPVDNA chip, which consisted of 16 high-risk HPV genotypes (oncogenic genotypes) and 8 low-risk HPV genotypes. The overall prevalence of HPV infection in this study population was 39.1% (258/660) and the 20's showed the highest prevalence of HPV infection (51.5%). The dominant HPV genotypes including single or multiple HPV-infected women were resulted in HPV-16 (15.9%), -53 (10.2%), -58 (7.7%), -18 (5.2%) in case of high-risk HPV genotype and HPV-70 (10.4%), -6 (4.1%), -11 (2.0%) in case of low-risk HPV genotypes. Remarkably, the proportion of women infected with high-risk HPV genotypes (62.0%) was almost four times higher than those of women infected with low-risk HPV genotypes (14.7%) and high/low-risk HPV genotypes (12.0%). Among the 258 HPV-infected women, single infection was 175, double infection 66, triple infection 12, quadruple infection 4, quintuple infection 1, respectively. Our finding suggests that the introduction and development of effective HPV vaccines should consider the current status of HPV genotypic infection in South Korean women.

Distribution of Human Papillomavirus 52 and 58 Genotypes, and Their Expression of p16 and p53 in Cervical Neoplasia

김태은,이경은,김활웅 대한병리학회 2014 Journal of Pathology and Translational Medicine Vol.48 No.1

Background: This study investigates the prevalence of human papillomavirus (HPV) 52 and 58 genotypes among women residing in Busan, and the expression of p16 and p53 proteins in cervical neoplasia with HPV 52 and 58 infections. Methods: A total of three hundred fifteen cases were analyzed using the HPV DNA chip test for HPV genotypes, and of these, we retrospectively examined p16 and p53 expression in 62 cases of cervical tissues infected with HPV 52 and 58 using immunohistochemistry. Results: HPV 52 and 58 genotypes were identified in 62 (54.9%) out of 113 high-risk, HPV-infected cases. Of the cases examined, there were 19 single HPV 52 infections (16.8%), 23 single HPV 58 infections (20.4%), 4 multiple HPV 52 infections (3.5%), and 16 multiple HPV-58 infections (14.2%). Immunoreactivity of p16 and p53 was observed in 41 (66.1%) and 23 (37.1%) of the 62 cases of cervical neoplasia infected with HPV 52 and 58 genotypes, respectively. Conclusions: This study demonstrates a high prevalence of HPV 52 and 58 genotypes, in addition to HPV 16, among high-risk strains of cervical neoplasia in Korea. These findings suggest that development of more vaccines would be beneficial for the prevention of the various HPV genotypes.