Ellagic Acid가 조골세포 분화에 미치는 영향

손효은(Hyo-Eun Son),김은아(Eunna Kim),변미순(Misoon Byun),장원구(Won-Gu Jang) 한국식품영양과학회 2019 한국식품영양과학회지 Vol.48 No.3

본 연구에서는 ellagic acid가 조골세포 분화에 미치는 영향을 조사하였다. 먼저 MTT assay를 통하여 세포독성 실험을 하였고 독성이 확인되지 않은 3 μM의 농도에서 실험을 진행하였다. 그리고 조골세포로 분화할 수 있는 중간엽 줄기세포인 C3H10T1/2 세포에 ellagic acid를 처리하여 BMP2, Runx2, ALP의 발현을 확인하였다. 또한 ellagic acid는 BMP2, Dlx5, Runx2의 단백질 발현을 증가시킴을 확인하였다. 그리고 ALP 활성 및 Alizarin Red S 염색을 확인한 결과 ellagic acid 처리에 의해서 ALP 활성이 현저히 증가하고 칼슘 침착 또한 증가하는 것으로 확인하였다. 뿐만 아니라 LPS에 의한 조골세포 분화의 감소가 ellagic acid에 의해 회복됨을 확인하였다. 이러한 결과들을 토대로 천연 폴리페놀 화합물인 ellagic acid는 조골세포 분화를 촉진한다는 것을 알 수 있고, ellagic acid는 골 질환 예방 가능성이 있을 것으로 생각된다. Ellagic acid is a compound found widely in strawberries, raspberries, and green tea and has been reported to have anti-oxidant, anti-inflammatory and anti-cancer activities. In previous reports, anti-oxidants induced osteoblast differentiation via the expression of osteogenic genes. This study examined the effect of ellagic acid on osteoblast differentiation using C3H10T1/2 cells. First, a MTT assay was performed to determine the concentration at which ellagic acid does not exhibit cytotoxicity. The cells were treated at a concentration of 3 μM without cytotoxicity. The effects of ellagic acid on the mRNA expression levels of the osteogenic genes in C3H10T1/2 cells were determined by real-time PCR. Ellagic acid increased the expression of bone morphogenetic proteins 2 (BMP2), runt-related transcription factor 2 (Runx2), and alkaline phosphatase (ALP). In addition, ellagic acid induced the protein expression levels of BMP2, distal-less homeobox 5 (Dlx5), and Runx2 according to a western blots assay. Next, a staining assay was performed to evaluate the ALP activity or mineralization. Ellagic acid enhanced both the ALP activity and mineralization. In addition, ellagic acid recovered lipopolysaccharide-induced osteoblast differentiation. These results suggest that ellagic acid may enhance osteoblast differentiation.

Ellagic Acid Shows Different Anti-proliferative Effects Between the MDA-MB-231 and MCF-7 Human Breast Cancer Cell Lines

Hyun-Ah Kim,이령아,문병인,Kuk-Jin Choe 한국유방암학회 2009 Journal of breast cancer Vol.12 No.2

Purpose: It has been demonstrated ellagic acid can inhibit tumor growth. However, the mechanism that elicits the antiproliferative effect of ellagic acid is poorly understood. Our objective in this study was to evaluate the biological activity of ellagic acid by comparing the anti-proliferative effect and the apoptotic pathway of ellagic acid between the 2 human breast cancer cell lines. Methods: The MCF-7 and MDAMB-231 human breast cancer cell lines were used as cell models. The anti-proliferstive effect was evaluated by using a MTT assay. The cell cycle was analyzed by flow cytometry. Western blotting was performed to show the expressions of bcl-xL, cytochrome c, surviving, c-fos and pS2. Results: The ellagic acid in the MDA-MB-231 cells showed significant antiproliferative effects with dose dependent pattern. The antiproliferative effects in MCF-7 cells were observed in only at a high concentration. Ellagic acid had no effect on the cell cycle in both breast cancer cells. In MDA-MB-231, the expression of bcl-xL was decreased with the decreasing concentration of ellagic acid. The expression of cytochrome c in the cytosol was increased with the decreased expression of bcl-xL. Ellagic acid also decreased the expression of survivin. In the MCF-7 cells, the expressions of bcl-xL and cytochrome c showed no change after treatment with ellagic acid even at a high dose. Ellagic acid was able to induce an upregulation of c-fos and pS2 protein in MCF-7. Conclusion: Ellagic acid has an anti-proliferative effect in the MDA-MB-231 cells. This effect of ellagic acid is through the intrinsic pathway in MDA-MB-231 cells. However, the expression of bcl-xL showed no change in the MCF-7 cells. Ellagic acid has a different anti-proliferative effect between the two human breast cancer cell lines.

성숙도에 따른 복분자 열매의 미백 활성 비교

박정용(Jeong-Yong Park),이지연(Ji Yeon Lee),서경혜(Kyung Hye Seo),장귀영(Gwi Young Jang),이승은(Seung Eun Lee),지윤정(Yun-Jeong Ji),김형돈(Hyung Don Kim) 한국영양학회 2020 Journal of Nutrition and Health Vol.53 No.2

본 연구에서는 복분자 열매의 성숙도에 따른 미백 활성의 차이를 확인하기 위해 B16F10 cell에서 melanin 생성량을 측정하고, 기능성분인 ellagic acid의 함량 변화를 확인하였다. 또한 ellagic acid의 함량에 따른 tyrosinase 저해 활성도 측정하였다. B16F10 cell에서 MRF 및 IRF 추출물은 200 ㎍/mL 농도까지 세포 독성을 나타내지 않았고, Melanin 생성량은MRF 추출물에 비해 IRF 추출물에서 더 좋은 억제 활성을 보여주었다. 또한 복분자 성숙도에 따른 ellagic acid의 함량 변화를 확인한 결과, MRF 추출물에 비해 IRF 추출물에서 ellagic acid의 함량이 높게 측정되었고, tyrosinase 저해 활성 평가에서도 MRF 추출물에 비해 IRF 추출물에서 더 좋은 억제 활성을 보였다. 결과를 종합해보면, MRF 추출물에 비해 IRF 추출물에서 ellagic acid의 함량이 더 높았으며, 이로 인해 tyrosinase의 저해 및 melanin 생성 억제 또한 IRF 추출물에서 더 좋은 것으로 판단된다. 따라서, 복분자 미성숙과는 천연 미백 소재로써 우수한 기능성 화장품 원료가 될 것이라 생각한다. Purpose: The Rubus coreanus fruit (RF) is an important traditional medicinal herb having antioxidant, anti-inflammatory, and immunoregulatory properties. These activities are known to change dramatically, depending on maturity of the RF. It is presumed that change of functional components, such as flavonoids, tannins, phenolic acids, triterpenoids and organic acids in RF, affect the various bioactivities. This study aimed to confirm changes in the anti-melanogenic effects of RF based on maturity, and to identify the bioactive compounds responsible. Methods: The cell viability of mature RF (MRF) and immature RF (IRF) extracts was investigated using B16F10 cells. To compare the anti-melanogenic effect of MRF and IRF extracts, we first assessed the melanin content. High-performance liquid chromatography analysis was performed to evaluate changes in the level of ellagic acid according to maturity of the RF. In addition, tyrosinase inhibitory activity of both extracts was examined. Results: MRF and IRF extracts (50–200 ㎍/mL) do not affect the cell viability of B16F10 melanoma cells. IRF extract more effectively inhibited melanin synthesis than MRF extract. The content of ellagic acid in IRF extract was higher than that obtained in MRF extract. Furthermore, greater inhibition of tyrosinase activity was observed after exposure to IRF extract than MRF extract. A positive correlation was determined between ellagic acid content and tyrosinase inhibitory activity, and a negative correlation was obtained between ellagic acid content and melanin content. Taken together, our results indicate that ellagic acid is one of the major bioactive compounds of RF that imparts a whitening effect. Conclusion: Our results indicate that ellagic acid in MRF and IRF extracts affect the antimelanogenesis effect through inhibition of tyrosinase activity. Therefore, the ellagic acid rich IRF has greater potential for application as a natural and functional cosmetic material.

UVA가 유도하는 산화적 스트레스에 Ellagic Acid가 미치는 인간 모유두 세포 보호효과

김경숙,한송희,안인숙,안규중 한국피부과학연구원 2016 아시안뷰티화장품학술지 Vol.14 No.2

Purpose: One of solar radiation components, ultraviolet A (UVA), has deeply penetrating capacity and generates reactive oxygen species (ROS) in the skin. These characteristics arouse oxidative stress in dermis, such as photoproducts, senescence, cell death, inflammation, and alterative gene expression. Thus, UVA is considered a major factor of external stimuli that irritates the skin. This study aimed at investigating the antioxidant and anti-inflammatory effects of ellagic acid on UVA-irradiated human dermal papilla cells (HDPCs). Methods: The cell viability of HDPCs were analyzed using the water-soluble tetrazolium salt (WST-1) assay. 2,7-dichlorofluorescin diacetate (DCFH-DA) was used in evaluating intracellular ROS scavenging activity. All the mRNA expression levels were carried out via quantitative real-time polymerase chain reaction (qRT-PCR). Cellular senescence was evaluated using senescence-associated β-galactosidase (SA-β-gal) staining kit. The wound healing assay in HDPCs were performed to assess effects of ellagic acid on the cell migration activity. Results: No cell cytotoxicity of ellagic acid was observed at concentration up to 25 μM. In irradiated HDPCs, ellagic acid showed protective effects on UVA-induced cytotoxicity. Further, ellagic acid represented ROS scavenging activity and regulating antioxidant gene expression, catalase transcriptional levels under UVA-irradiated condition. It also modulated tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) mRNA expresison levels and cellular senescence on UVA-stimulated HDPCs. Cell migration assay showed the protective effects of ellagic acid on movement of HDPCs damaged by UVA. Conclusion: These results demonstrate that ellagic acid protects UVA-induced oxidative stress in HDPCs. 목적: 태양광을 구성하는 성분 중 하나인 UVA는 피부에서 활성산소(reactive oxygen species, ROS를) 생산하고 피부 깊숙히 침투할 수 있는 능력을 가지고 있다. 이러한 특성은 진피층에 광분해생성물이나 세포노화, 세포 사멸, 염증, 유전자 발현 변화와 같은산화적 스트레스를 유발한다. 따라서, UVA는 피부를 자극하는 주요 외부인자로 여겨지고 있다. 본 연구의 목적은 UVA에 의한자극에 ellagic acid의 항산화 및 항염 효과를 인간 모유두 세포를 이용하여 확인하였다. 방법: 세포 생존력은 WST-1 방법을 사용하여 수행하였으며, DCFH-DA 을 이용하여 세포 내 활성산소 소거능을 연구하였다. 본 연구에서 시험한 모든 mRNA 발현은 qRT-PCR을 통해 분석하였으며 세포노화는 SA-β-gal 세트를 사용하여 조사하였다. 세포이동능 분석을 통해 ellagic acid가 HDPC의 이동능에 미치는 영향을 알아보았다. 결과: Ellagic acid 25 μM까지 세포독성은 확인되지 않았으며, UVA로 인한 세포독성에 ellagic acdi가 보호하는 효과를 확인하였다. 더불어, 항산화 효소인 CAT의 유전자 발현변화를 유도하는 효과와 세포 내 활성산소를 소거하는 효과를 실험을 통해 입증하였다. 이뿐만 아니라, UVA 조사로 인해 발현이 증가한 염증유도인자, TNF-α, IL-6의 mRNA 발현 수준을 조절하는 것으로 나타났으며UVA로 인해 손상된 세포의 세포 이동능을 보호하는 것에도 유의적인 효과가 있음을 확인할 수 있었다 . 결론: 본 연구결과는 ellagic acid가 UVA로 인해 유도되는 산화적 스트레스를 보호하는 효과를 인간 모유두 세포를 이용하여 입증하였다.

고창 복분자의 기능성원료 표준화를 위한 지표성분으로서 Ellagic Acid의 분석법 개발

김윤정(Yunjeong Kim),한송희(Song-Hee Han),전지영(Ji-Yeong Jeon),황민호(Minho Hwang),임용진(Yong-Jin Im),채수완(Soo-Wan Chae),김민걸(Min-Gul Kim) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

고창 복분자의 개별인정형 건강기능식품 기능성 원료로 개발하기 위하여 지표성분 표준화를 위한 ellagic acid의 분석법 설정과 분석법에 대한 밸리데이션을 실시하였다. 1% formic acid가 첨가된 water와 acetonitrile을 이동상으로 하고 Symmetry®(C18, 4.6×250 ㎜, 5.0 ㎛) 칼럼을 사용하여 기울기용리(gradient elution) 방법으로 분석하였다. 본 연구에서는 분석법을 확립하고 분석법에 대하여 특이성, 직선성, 정확성과 정밀성 그리고 회수율에 대하여 확인하였다. Ellagic acid의 검량선은 R2=0.9996으로 좋은 선형성을 보였으며 LLOQ와 LOD는 각각 1 ㎍/mL와 0.3 ㎍/mL였다. 일내와 일간 분석에서 상대표준편차(RSD)는 각각 2.28%와 2.84% 미만으로 나왔다. 회수율 측정결과에서는 89.17~97.92%로 나왔고, RSD는 0.05~0.14%였다. 그러므로 HPLC를 이용한 ellagic acid의 분석법이 고창 복분자 추출물의 기능성원료 표준화를 위한 지표성분 분석을 위한 적합한 시험법임이 검증되었다. 본 시험법에 따라 분석한 고창 미숙과 복분자 추출물 내의 ellagic acid의 함량은 세 lot를 3번씩 분석하였을 때 약 1.92 ㎍/㎎(0.192%)이 나왔으며 RSD값은 2.36% 이하였다. 따라서 본 연구를 통하여 확립된 ellagic acid의 분석법이 고창 복분자의 개별인정형 건강기능식품 기능성 원료개발을 위한 기초자료로 활용될 것으로 본다. Method development and validation of ellagic acid for the standardization of Gochang Bokbunja as a functional ingredient and health food were accomplished. A Symmetry® (C18, 4.6×250 ㎜, 5.0 ㎛) column was used with a gradient elution system of 1% formic acid in water and acetonitrile. This method was validated according to specificity, linearity, accuracy, precision test, and recovery test. Specificity was confirmed with identical retention time, and calibration curves of ellagic acid showed good linear regression (R2>0.9996). Relative standard deviations (RSD) of data from the intra- and inter-day experiments were less than 2.28% and 2.84%, except in the low limit of quality control (LLOQ, 1 ㎍/mL) sample. The results of the recovery test were from 89.17% to 97.92% with RSD values from 0.05 to 0.14%. Therefore, we performed analysis of ellagic acid as a marker compound in Gochang Bokbunja extracts. The amount of ellagic acid in Gochang Bukbunja was about 1.918 ㎍/㎎ (0.192%) in the three times analysis, and RSD was less than 2.36% by the validated method. These results suggest that the developed HPLC method is simple, efficient, and could contribute to the quality control of Gochang Bokbunja extract as a functional ingredient.

인간모유두세포에서 자외선A에 대한 세포손상에 Ellagic Acid의 세포보호 효과 및 세포보호기전 분석

김경숙 대한피부미용학회 2015 대한피부미용학회지 Vol.13 No.6

This study observed change in expression of transcription of genes, p53 and downstream of p53 in HDPCs that were exposed with UVA, researched into the restraining effect of ellagic acid on cell cycle arrest by UVA and the cell activation effect of ellagic acid, hence implemented in order to provide baseline date for future product development of hair and scalp care products using ellagic acid in HDPCs. In this study, it was proven that ellagic acid reduced occurring cell cycle arrest due to UVA on HDPCs. It was proven that a tumor suppressor gene p53 , which was activated upon DNA damage and cell malfunction and was contributed to cell cycle arrest or apoptosis, was increased by UVA, otherwise it was decreased by ellagic acid treatment. It was found that another tumor suppressor gene Gadd45α and 14-3-3σ also were increased by UVA, to the contrary they were decreased by ellagic acid treatment. Both tumor suppressor genes were a factor which would be avail of adjustment to G2/M arrest could generate G2/M arrest and play a role of repairing DNA. Explaining in other words, it was revealed that ellagic acid worked by repairing DNA and decreasing occurring tumor suppressor genes. UVA on HDPCs generated G2 arrest through the course of synthesizing DNA and passing G2 phase and entering M phase and eventually decreasing Cyclin B occurrence. Results from this study indicates that ellagic acid has a protective effect on human dermal papilla cells where UVA damages. Through which, it is expected that understanding of analysis on ellagic acid’s cell reaction, p53 and p53 downstream molecule, G2/M cell cycle arrest will be of avail for supporting further development of scalp and hair products by employing ellagic acid because it can be used as a basic information and a row material for cosmetics.

Quantitative Analysis and <i>In vitro</i> Anti-inflammatory Effects of Gallic Acid, Ellagic Acid, and Quercetin from Radix Sanguisorbae

Seo, Chang-Seob,Jeong, Soo-Jin,Yoo, Sae-Rom,Lee, Na-Ri,Shin, Hyeun-Kyoo Medknow PublicationsMedia Pvt Ltd 2016 Pharmacognosy magazine Vol.12 No.46

<P><B>Background:</B></P><P>Radix Sanguisorbae has long been used to treat diarrhea, enteritis, duodenal ulcers, and internal hemorrhage.</P><P><B>Objective:</B></P><P>We investigated the <I>in vitro</I> anti-inflammatory effects of Radix Sanguisorbae and performed quantitative analyses of three marker components, namely gallic acid, ellagic acid, and quercetin, using high-performance liquid chromatography coupled with a photodiode array detector.</P><P><B>Materials and Methods:</B></P><P>The three marker components were separated using a reversed-phase Gemini C<SUB>18</SUB> analytical column maintained at 40°C by the gradient elution with two solvent systems. We examined the biological effects of the three marker compounds, gallic acid, ellagic acid, and quercetin, by determining their anti-inflammatory activities in the murine macrophage cell line RAW 264.7.</P><P><B>Results:</B></P><P>All of the marker compounds exhibited inhibitory effects on prostaglandin E<SUB>2</SUB> production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, with no cytotoxicity. Particularly, ellagic acid significantly inhibited production of the proinflammatory cytokines tumor necrosis factor alpha and interleukin-6 in LPS-treated RAW 264.7 cells.</P><P><B>Conclusion:</B></P><P>Our results suggest that ellagic acid is the most potent bioactive phytochemical component of radix Sanguisorbae in the treatment of inflammatory diseases.</P><P><B>SUMMARY</B></P><P><P>Established high-performance liquid chromatography method was applied in the quantitative analysis of gallic acid, ellagic acid, and quercetin present in an extract from radix Sanguisorbae</P><P>Among the three compounds, the ellagic acid.(7.65.mg/g) is main component in radix Sanguisorbae</P><P>Ellagic acid significantly inhibited production of the proinflammatory cytokines tumor necrosis factor alpha and interleukin-6 in lipopolysaccharide-treated RAW 264.7 cells.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviations used:</B> HPLC: High-performance liquid chromatography, PDA: Photodiode array, TNF-α: Tumor necrosis factor alpha, IL: Interleukin, LPS: Lipopolysaccharide, PGE<SUB>2</SUB>: Prostaglandin E<SUB>2</SUB>, NSAIDs: Nonsteroidal anti-inflammatory drugs, COX: Cyclooxygenase.</P>

(PIM-co-Ellagic Acid)-기반의 이산화탄소 분리막의 개발

호세인 이크발,허스너 아스몰,김동영,김태현 한국막학회 2020 멤브레인 Vol.30 No.6

(PIM-1)과 ellagic acid로 만든 랜덤형 공중합체가 간단한 방법으로 합성되었으며, 이산화탄소 분리막에 대한 적용 가능성에 대해서 연구하였다. 이 공중합체의 경우 PIM (polymers with intrinsic microporosity) 고분자의 미세 기공 구조에 기 인한 높은 기체 투과도와 평면 구조와 친수성을 갖는 ellagic acid에 기인한 높은 이산화탄소에 대한 선택성에 의해 우수한 이 산화탄소 기체 분리 성능을 나타내었다. 즉, 이산화탄소에 대한 투과도 4516 Barrer와 CO2/N2 (> 23~26) 및 CO2/CH4 (> 18~19)의 높은 선택성으로 두 쌍의 가스 혼합물에 대해 Robeson 상한(2008)을 초과한 결과를 나타내었다. 이와 같이 PIM-1 에 평면구조를 갖는 ellagic acid을 혼입하면 PIM-1의 꼬인 구조를 방해하여 기체 투과성을 향상 시킬 뿐만 아니라 공중합체 의 강성과 극성이 증가하여 N2 및 CH4에 대한 CO2의 선택성을 증가시키는 결과를 확인하였다. Random copolymers made of both ‘polymer of intrinsic microporosity (PIM-1)’ and Ellagic acid were prepared for the first time by a facile one-step polycondensation reaction. By combining the highly porous and contorted structure of PIM (polymers with intrinsic microporosity) and flat-type hydrophilic ellagic acid, the membranes obtained from these random copolymers [(PIM-co-EA)-x] showed high CO2 permeability (> 4516 Barrer) with high CO2/N2 (> 23~26) and CO2/CH4 (> 18~19) selectivity, that surpassed the Robeson upper bound (2008) for both pairs of the gas mixture. Incorporation of flat-type ellagic acid into the PIM-1 not only enhances the gas permeability by disturbing the kinked structure of PIM-1 but also increases the selectivity of CO2 over N2 and CH4, due to an increase of rigidity and polarity in the resultant copolymer membranes.

식품 중 Ellagic acid의 발암수식효과

장동덕,신동환,흥충만,조재천,한정희 한국식품위생안전성학회 1998 한국식품위생안전성학회지 Vol.13 No.1

의약품과 식품을 포함한 자연이나 환경속에 포함되어 있는 여러 가지 화학물질들은 암 및 돌연변이를 유발할 수 있기 때문에 이런 것들에 의한 유해작용을 줄이려 노력하고 있으나 완전히 없애기 어려운 현실이다. 따라서 식생활 습관을 개선하거나 식품내에 존재하는 발암억제물질을 이용하여 암의 발생된 위한 연구는 암의 치료제 개발과 더불어 관심의 대상ㅇ디 되고있다. 여러 가지 식품속에 자연적으로 함유된 ellagic acid는 항돌연변이와 함암역제 효과가 있는 것으로 잘 알려져 있다. 따라서 본 실험에서는 단기간 ellagic acid의 발암억제효과를 알아보기위하여 전암지표효소인 GST-P 양성중식소를 측정하였다. Diethylnitrosamine으로 간장에서 암을 유발하였고 phenobarbital과 간부분절제술로 암을 촉진시켰으며, ellagic acid를 400과 800ppm 투여근으로 구분하고 투여시기를 달리하여 실험하였다. 따라서 diethylnitrosamine으로 유발된 간장의 긴장의 발암은 ellagic acid에 의해 용량의존적으로 억제됨을 알 수 있다. The effect of ellagic acid (EA) on hepatocarcinogenesis induced by diethylnitrosamine (DEN), and promoted by phenobarbital (PB), and hepatectomized partially was investigated in male Wistar rats. All rats were injected 200 mg of DEN intraperitoneally, received 0.05% of PB in drinking water at week 2, and hepatectomized 2/3 of liver at week 3. Rats of group 2, 3 and 4 were fed diet containing 400 ppm Ea for 1week before DEN administration, for 9 weeks from beginning of experiment to sacrifce and for 6 weeks from p treatment to sacrifice respectively. Rats of group 5, 6 and 7 were fed 800 ppm Ea in the sam e manner as group 2, 3 and 4. plastic lesions were quantified the glutathione-S-transferase placental-form (GST-P) positive foci served in the rats fed 400 ppm EA for 9 weeks. In addition, the reduction of the foci can examine in all group fed 800pm EA. In conclusion, Ea inhibited the hepatocarcinogenesis induced by den when it was administrated 800 ppm.

국내유통 복분자와 토종복분자의 이화학적 특성과 엘라그산 함량 비교연구

정성희(Sung Hee Jung),유혜영(Hye Young Yu),서지호(Ji Ho Seo),이용재(Yong Jae Lee),한민우(Min Woo Han) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.2

The purpose of this study was to compare the chemical properties of the bokbunja distributed in Korea with the content of the bioactive substance ellagic acid. The bokbunja was Rubus coreanus group and Rubus occidentalis group were compared, domestic bokbunja and import bokbunja were compared. In bokbunja, free sugar was 30.89 ± 0.7 ㎎/g of Rubus coreanus and 29.05 ± 0.87 ㎎/g of Rubus occidentalis. and 27.28 ± 7.4 ㎎/g of domestic bokbunja and 21.58 ± 6.73 ㎎/g of import bokbunja. The free amino acids was 4.50 ± 0.08 ㎎/g of Rubus coreanus and 5.05 ± 0.08 ㎎/g of Rubus occidentalis. and 4.13 ± 1.09 ㎎/g of domestic bokbunja and 3.75 ± 0.31 ㎎/g of import bokbunja. Validation of the ellagic acid method was confirmed by comparing the retention time and spectrum of the standard and extract using HPLC. The calibration curve (R2) showed linearity of 0.9999. As a result of analyzing the ellagic acid content of each extraction solvent, DMSO and methanol mixture extracts were high, and Rubus coreanus was 2.56 ㎎/g and Rubus occidentalis was 3.16 ㎎/g, which was not significantly different (p < 0.05) In addition, the ellagic acid content of domestic bokbunja and import bokbunja was 2.83 ㎎/g and 2.99 ㎎/g, which was not significantly different (p < 0.05).