Genetic Resources Management with Networks: the Example of the Network "Carrot and Other Daucus"

Mathilde Briard,Valerie Le Clerc,Didier Peltier,Anita Suel,Jacky Granger,Cecile Dubois,Emmanuel Geoffriau 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

당근 추출 성분의 Quinone reductase 유도활성 효과

한은주,노승배,배송자 한국생명과학회 2000 생명과학회지 Vol.10 No.1

Chemoprevention is one of the major strategies for cancer control. It is well established that dietary factors play an important role in modulating the development of certain types of human cancer. The experiment was conducted to determine quinone reductase(QR) activity induction of Daucus carota L. on HepG2 cells. Among various partition layers of roots of Daucus carota L., the ethyl acetate partition layer(DCMEA) and the n-hexane partition layer(DCMH) tested to be most effective which resulted 2.1 and 1.6 respectively compared to the control value of 1.0. In the case of seeds of Daucus carota L. n-butanol partition layer (DCMB) on HepG2 cells at a dose of 200 $\mu\textrm{g}$/$m\ell$ showed the highest induction activity of QR which was 3.0. These results suggest that potentially useful cancer chemoprevention chemicals could be isolated from DCMEA and DCMH of the roots and DCMB of the seeds of Daucus carota L.

NO와 Pro-Inflammatory Cytokine의 억제를 통한 호라복(胡蘿蔔)의 항염증효과

이동진,박상미,황보민,정태영,김상찬,지선영,Lee, Dong-Jin,Park, Sang-Mi,Hwangbo, Min,Jung, Tae-Young,Kim, Sang-Chan,Jee, Seon-Young 대한한방안이비인후피부과학회 2013 한방안이비인후피부과학회지 Vol.26 No.2

Objectives : Daucus carota sativa has been frequently used as food supplements in many of the Asian countries, and a nutritional medical drug in traditional medicine. This research investigated the effects of Daucus carota sativa extract (DCE) on acute phases of inflammation in Raw 264.7 cells treated with lipopolysaccharide (LPS) in terms of the inhibition of nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$) and pro-inflammatory cytokines production. Methods : NO, $PGE_2$, tumor necrosis factor (TNF)-${\alpha}$, interleukin-$1{\beta}$ and interleukin-6 contents were assayed by ELISA, and expressions of inflammation-related proteins such as inducible NO synthase (iNOS) were determined by immunoblot analyses. Results : DCE treatment attenuated the LPS ability to increase the productions of NO and $PGE_2$ as well as the protein level of iNOS in a concentration-dependent manner. Consistently, treatment of the cells with DCE suppressed the production of TNF-${\alpha}$, interleukin-$1{\beta}$ and interleukin-6. DCE also caused decreases of inhibitor of ${\kappa}B{\alpha}$ phosphorylation induced by LPS in the cells, which means DCE inhibition of NF-${\kappa}B$ activity. Furthermore, DCE blocked LPS-induced phosphorylation of p38 and SAPK/JNK. Conclusion : This study showing here may be of help to understand the action mechanism of DCE, and provide the information for the medical use of Daucus carota sativa for the inflammatory disease.

당근의 체세포배로부터 발생한 경정부의 구조적 특성

홍성식,소웅영 한국자원식물학회 1999 한국자원식물학회지 Vol.12 No.2

당근(Daucus carota L. cv. Hongshim)의 접합자배와 체세포배의 경정부의 구조는 주사전자현미경과 종단면 관찰을 통하여 이루어졌다. 접합자배의 경정부는 도립된 보우트형이고, 2ㆍ3ㆍ4자엽 체세포배의 경정부는 각각 도립된 보우트형, 피라미드형 및 볼록한 다이아몬드형으로 나타났다. 접합자배의 경정부는 다소 작은 세포들로 구성되어 있고, 초층과 내체의 구별이 된다. 체세포배의 경정부는 다소 큰 세포들이 불규칙하게 배열되어 있다. The structure of shoot apex in zygotic and somatic embryos of Daucus carota L. cv. Hongshim was observed by using SEM and longitudinal sections. Shoot apex of zygotic embryo was of an inverted boat shape, and these of two, three and four cotyledon somatic embryos were of an inverted boat shape, a pyramid shape and a convex diamond shape, respectively. In zygotic embryo shoot apex is consisted of small cells which are arranged in layers (tunica) and show corpus in central region. In somatic embryos shoot apices are consisted of somewhat large cells which are arranged in irregularly or slight regularly.

Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways

Wassim N. Shebaby,K.B. Bodman-Smith,Anthony Mansour,Mohamad Mroueh,Robin I. Taleb,Mirvat El-Sibai,Costantine F. Daher 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.7

Daucus carota L. ssp. carota (Apiacea, wild carrot, Queen Anne’s lace) has been used in folk medicine throughout the world and recently was shown to possess anticancer and antioxidant activities. This study aims to determine the anticancer activity of the pentane fraction (F1) and the 1:1 pentane:diethyl ether fraction (F2) of the Daucus Carota oil extract (DCOE) against human colon adenocarcinoma cell lines (HT-29 and Caco-2). Treatment of cells with various concentrations of F1 or F2 fractions produced a dose-dependent inhibition of cell proliferation. Flow cytometric analysis indicated that both fractions induced sub-G1 phase accumulation and increased apoptotic cell death. Western blot revealed the activation of caspase-3, PARP cleavage, and a considerable increase in Bax and p53 levels, and a decrease in Bcl-2 level. Treatment of HT-29 cells with either fraction markedly decreased the levels of both phosphorylated Erk and Akt. Furthermore, the combined treatment of F1 or F2 with wortmannin showed no added inhibition of cell survival suggesting an effect of F1 or F2 through the phosphatidyl inositol 3-kinase (PI3K) pathway. This study proposes that DCOE fractions (F1 and F2) inhibit cell proliferation by inducing cell cycle arrest and apoptosis in HT-29 cells through the suppression of mitogen-activated protein kinase (MAPK)/Erk and PI3K/Akt pathways.

야생당근(Daucus carota L.) 주스를 첨가한 개호식품(젤리) 제조 및 품질 특성

강희선 ( Hee-sun Kang ),김민주 ( Min-ju Kim ),노정옥 ( Jeong-ok Rho ),최형일 ( Hyong-il Choi ),한명륜 ( Myung-ryun Han ),명정호 ( Jeung-ho Myung ),김애정 ( Ae-jung Kim ) 대한영양사협회 2017 대한영양사협회 학술지 Vol. No.

This study evaluated the quality characteristics of jelly prepared with different levels (0%, 5%, 10%, 15%, 20%, and 25%) of wild carrot (WC, Daucus carota L.) juice as a care food for the elderly. The lightness, redness, yellowness, and delta (Δ) values of the jelly (Control, WCJ5, WCJ10, WCJ15, WCJ20, and WCJ25) decreased with increasing amounts of wild carrot juice added. The mechanical properties, such as hardness, springiness, chewiness, and gumminess, of the jelly were decreased with increasing amounts of wild carrot juice added. The total polyphenol and total flavonoid contents of the jelly increased with increasing amounts of wild carrot juice added. The DPPH radical scavenging activity (IC₅₀) also increased with increasing amounts of wild carrot juice added. The α-glucosidase inhibitory effects of wild carrot (WC) and WCJ25 were 71% and 54.4%, respectively, compared to the positive control (acarbose). The lipase inhibitory effects of WC and WCJ25 were 44.2% and 14.4%, respectively, compared to the positive control group (orlistat). On the other hand, the sensory evaluation score was the best at WCJ20, which contained 20% wild carrot juice. In conclusion, WCJ20 or WCJ25 is expected to be a care food for the elderly with respect to texture as well as the antioxidant and enzymatic activity (α-glucosidase inhibitory and lipase inhibitory activities).

야생당근(Daucus carota L.) 주스를 첨가한 개호식품(젤리) 제조 및 품질 특성

강희선,김민주,노정옥,최형일,한명륜,명정호,김애정,Kang, Hee-Sun,Kim, Min-Ju,Rho, Jeong-Ok,Choi, Hyong-Il,Han, Myung-Ryun,Myung, Jeung-Ho,Kim, Ae-Jung 대한영양사협회 2017 대한영양사협회 학술지 Vol.23 No.4

This study evaluated the quality characteristics of jelly prepared with different levels (0%, 5%, 10%, 15%, 20%, and 25%) of wild carrot (WC, Daucus carota L.) juice as a care food for the elderly. The lightness, redness, yellowness, and delta (${\Delta}$) values of the jelly (Control, WCJ5, WCJ10, WCJ15, WCJ20, and WCJ25) decreased with increasing amounts of wild carrot juice added. The mechanical properties, such as hardness, springiness, chewiness, and gumminess, of the jelly were decreased with increasing amounts of wild carrot juice added. The total polyphenol and total flavonoid contents of the jelly increased with increasing amounts of wild carrot juice added. The DPPH radical scavenging activity ($IC_{50}$) also increased with increasing amounts of wild carrot juice added. The ${\alpha}$-glucosidase inhibitory effects of wild carrot (WC) and WCJ25 were 71% and 54.4%, respectively, compared to the positive control (acarbose). The lipase inhibitory effects of WC and WCJ25 were 44.2% and 14.4%, respectively, compared to the positive control group (orlistat). On the other hand, the sensory evaluation score was the best at WCJ20, which contained 20% wild carrot juice. In conclusion, WCJ20 or WCJ25 is expected to be a care food for the elderly with respect to texture as well as the antioxidant and enzymatic activity (${\alpha}$-glucosidase inhibitory and lipase inhibitory activities).

HepG2 세포에서 AMPK 활성화를 통한 胡蘿蔔 에탄올 추출물의 간 세포 보호 효과

김도연,박상미,변성희,박정아,조일제,김상찬 대한한의학방제학회 2018 大韓韓醫學方劑學會誌 Vol.26 No.4

Objectives : In Traditional Korean medicine, Daucus carota L. has been used for treating dyspepsia, diarrhea, dysentery and cough. Recent pharmacognosic evidence showed D. carota has anti-oxidant, anti-cancer, anti-fungal, and hypotensive effects. Present study investigated hepato-protective effect of D. carota ethanol extract (DCE) against oxidative stress in HepG2 cells. Methods : After HepG2 cells were pretreated with different concentrations of DCE, the cells were exposed to tert-butyl hydroperoxide (tBHP) for inducing oxidative stress. Cell viability, hydrogen peroxide production, glutathione concentration, and mitochondrial membrane potentials were measured to explore hepato-protective effect of DCE. Phosphorylation of AMP-activated protein kinase (AMPK) and effect of compound C on cell viability were determined to investigate the role of AMPK on DCE-mediated cytoprotection. Results : DCE significantly decreased the tBHP-mediated cytotoxicity in a concentration dependent manner and reduced the changes on apoptosis-related proteins by tBHP in HepG2 cells. In addition, DCE significantly prevented hydrogen peroxide production, glutathione depletion, and mitochondrial membrane impairment induced by tBHP. Treatment with DCE increased phosphorylation of AMPK, and the DCE-mediated cytoprotection was abolished by pretreatment with compound C. Conclusions : These results demonstrate that DCE can protect hepatocytes from oxidative stress through activation of AMPK.

Cardiovascular Actions of Daucus carota

Gilani, A.H.,Shaheen, F.,Saeed, S.A. The Pharmaceutical Society of Korea 1994 Archives of Pharmacal Research Vol.17 No.3

Ethanolic extract of Daucus carota (DC) at the dose of 10-100 mg/kg caused a dose-dependent fall in systolic and diastolic arterial blood pressure in nomotensive anesthetized rats. These effects were not blocked by atropine (1 mg/kg) and pretratment with DC did not alter the pressor response to norepinephrine indicating that cardiovascular effects of DC are independent of cholinergic or adrenergic recptors involvement. In spontaneously beating guinea-pig paired atria, DC induced a concentration-dependent (03-5 mg/ml) decrease in force and rate of atrial contractions. In rabbit thoracic aorta, DC caused inhibition of $K^+$-induced contractions at similar concentrations. These results suggest that the extract may exhibit $Ca^{2+}$ channel blocking-like direct relaxant action on cardiac and smooth muscle preperations and this action may be responsible for its hypotensive effect observed in the in vivo studies.

자색당근 첨가가 돈육 햄버거 패티의 품질에 미치는 영향

고영주,유승석,Ko, Young-Joo,Yoo, Seung-Seok 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.3

The purpose of this study was to investigate the effects of black carrot (Daucus carota L.) on the quality characteristics and antioxidant activity of pork patties. Patties were provided with different amounts (0, 2, 4, 6 and 8%) of black carrot. Notably, the cooking loss rate of the group added with 8% black carrot was lowest (p<0.001). As the content of black carrot increased, the L value decreased and the a, b value increased. The texture analysis results showed that the hardness and chewiness to decrease as the amount of black carrot increased (p<0.05). Evaluation of consumer acceptability revealed that the 4% black carrot groups had a higher score in respect to overall preference and taste to the other groups (p<0.05). For antioxidant activities, both total phenolic compounds content and DPPH were increased (p<0.001) as the amount of added black carrot was increased. As a result, it can be concluded that adding the black carrot to patties in processing can improve antioxidant activities and quality of the patties. Considering various aspects of quality, the suitable amount is 4% of total weight.