당조고추 70% 에탄올추출물의 항산화 및 항암활성측정

이연리 ( Youn Ri Lee ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.5

당조고추의 기능성 소재로서의 가능성을 알아보기 위하여 당조고추 70% 에탄올 추출물에 대한 항산화 활성 및 항암활성을 측정하였다. 당조고추 70% 에탄올 추출물의 총 폴리페놀은 30.29 mg GAE/g extract로 나타났다. 당조고추 70% 에탄올 추출물에 대한 DPPH radical 및 hydroxyl radical 소거활성을 IC50% 측정한 각각 2.87, 10.55 mg/mL의 소거활성을 보여주었으며, α-glucosidase 및 α-amylase 저해활성은 10 mg/mL의 농도에서 각각 35.67, 58.41%로 나타났다. 당조고추 70% 에탄올 추출물에 대한 항암활성은 대장암 세포주의 0.5 mg/mL 농도에서 50% 암세포 성장억제를 보였지만, 다른 암세포에서는 억제효과를 나타내지 않았다. To evaluate the anti-oxidant and anti-tumor potential of the green pepper (Capsicum annuum L. cv. DangZo), total polyphenol content, radical scavenging activities and anti-tumor properties were measured. The total polyphenol content of the 70% ethanol extracts from green pepper (Capsicum annuum L. cv. DangZo) was 30.29 mg gallic acid equivalent/g extract. The DPPH radical and hydroxyl radical scavenging activities of 70% ethanol extracts of green pepper (Capsicum annuum L. cv. DangZo) were documented at 2.87 and 10.55, respectively. For α-glucosidase and α-amylase inhibitory activity, 70% ethanol extracts of green pepper (Capsicum annuum L. cv. DangZo) were documented at 35.67% and 58.41% respectively. The green pepper (Capsicum annuum L. cv. DangZo) demonstrated greater capability in terms of anti-neoplastic activity vis-a-vis colon cancer cell lines when compared to other cancer cell lines.s. er (Capsicum annuum L. cv. DangZo) higher activities of anticancer activities on colon cancer cell lines compared to other cancer cell lines.

고추 자엽에서 식물체 재분화의 품종간 차이

오명규,이영만,박문수 한국식물생명공학회 1998 식물생명공학회지 Vol.25 No.5

자엽으로 부터 신초 유기율은 MS기본배지와 MS기본배지에 IAA 0.25mg/L 또는 0.50mg/L과 zeatin 2.0mg/L 또는 4.0mg/L를 단독 및 조합 처리한 배지중에서 M배지에 IAA 0.25mg/L + zeatin 2.0mg/L 첨가된 배지가 63%로 가장 양호하였다. 신초 유기시기는 품종 및 배지에 따라 치상후 9~25일의 차이가 있었는데 MS배지 + zeatin 2.0mg/L + IAA 0.25 mg/L배지에서 영양재래, 풋고추, 342, Kakovskij-A-35, Gris I-A-1가 빠르게 유기 되었으며, MS배지 + BA 2.0mg/L + IAA 1.0mg/L 배지에서 COO485 Long Hot P5-3, Gris I-A-1에서 유기가 빨았다. 신초 유기율도 품종 및 배지에 따라 달랐는데 MS 배지 + zeatin 2.0mg/L + IAA 0.25mg/L 배지에서는 621, 영양재래, Nikko(일광), 적색물고추, Ch-6Num-216, 풋고추, Kakovskij-A-35가, MS배지 + BA 2.0mg/L + IAA 1.0mg/L 배지에서는 Fresno Chile, PI 169126, 적색물고추, PI 297438가 90%이상으로 높았다. Effects of genotype and culture medium on plant regeneration from cotyledon segments of red pepper(Capsicum annuum L.) was investigated. Among combinations of IAA(0.25 and 0.50 mg/L) and zeatin(2.0 and 4.0 mg/L) added to MS medium, combination of 2.0 mg/L zeatin and 0.25 mg/L IAA was shown to be the best for shoot differentiation from cotyledon segments. Shoot regeneration from cotyledon explants took 9 to 25days, depending on genotypes and culture media. Early shooting was observed in Yeongyangjaelae, Putgochw, Karkovskij-A-35, Gris I-A-1 on MS medium containing 2.0 mg/L zeatin and 0.25 IAA mg/L. Percent of explants producing shoots, as also influenced by genotypes and culture media, were over 90% for 621, Yeongyangjaelae, Putgochw, Nikko jacksacgmulgochw, Ch-6-Num-216, and Kajenskij-A-35 when cultured on MS medum supplemented with 2.0 mg/L zeatin and 0.25 mg/L IAA and for Fresno chile, PI 169126, Kajenskij-A-35, jacksacgmulgochw, and PI 297438 on MS medium including 2.0 mg/L BA and 1.0 mg/L IAA.

추출 조건에 따른 고춧잎 추출물의 항산화 활성 및 α-Glucosidase 억제 활성

서예슬,김숙정,김태한,이수홍,양은주 한국산업식품공학회 2022 산업 식품공학 Vol.26 No.2

This study investigated optimal extraction conditions for applying Capsicum annuum L. leaf as a functional food resource. Capsicum annuum L. leaf was extracted at different extraction solvents (water and 95% ethanol), extraction temperatures (80oC and 100oC), and e xtraction times ( 30, 60, and 9 0 min), a nd t he extracts were e valuated for extraction yield, luteolin content as a major flavonoid component, antioxidant activity, and α-glucosidase inhibitory activity. The extraction yield, luteolin content, DPPH and ABTS radical scavenging activities, and α-glucosidase inhibitory activity of the hot water extract were higher than those of the ethanol extract. In evaluating the extraction temperature of Capsicum annuum L. leaf, the antioxidant activities were similar, but the extraction yields, luteolin contents, and α-glucosidase inhibitory activities were higher at 100oC extraction. In evaluating the extraction time of Capsicum annuum L. leaf, extraction yield increased as the extraction time increased, but antioxidant activity and α-glucosidase inhibitory activity were the highest at 60 min of extraction. These results suggest that the optimum extraction conditions of Capsicum annuum L. leaf are hot water for 60 min at 100oC, and the extracts can be used as a functional food resource.

형질전환된 고추( Capsicum annum L.) 식물체의 Mouse Adenosine Deaminas 유전자 발현

양덕춘,이계연,유영숙,최경화,임학태 한국식물생명공학회 1997 식물생명공학회지 Vol.24 No.1

고추의 형질전환율을 높이기 위하여 우선적으로 효율적인 재분화조건을 구명하였다. 고추의 하배축과 자엽 모두 2mg/L zeatin과 0.1 mg/L NAA ( I )에서 51%, 1.0 mg/L BAP와 10.0 mg/L IBA ( II)는 45.1%의 재분화율을 보였으며, 두 배지에 5 $\mu$M AgNO$_3$을 첨가하였을 때 I의 배지에서 보다 건강한 식물체를, II의 배지에서는 재분화율이 약 8%로 증가함을 보였다. 따라서 II의 배지 조건은 가격이 비싼 zeatin의 효과를 대치할 수 있었다. 이렇게 얻어진 효율적인 재분화배지에 고추의 하배축과 자엽을 ADA와 NPT II 유전자를 함유한 Agrobacterium tumefaciens pDY183을 이용하여 형질전환을 유도하여, kanamycin 100 mg/L에서 선발하여 성공적으로 형질전환체를 얻었다. 식물체내로의 ADA와 NPT II 유전자의 도입은 PCR을 이용하여 확인하였으며, Northern blot에 의하여 ADA 유전자의 전사여부를 확인하였다. ADA 효소의 활성도는 spectrophotometer를 이용하여 측정하여 본 결과 고추세포내에서 정상적으로 발현하였으므로 동물유전자인 ADA가 식물체 형질전환시 표시 유전자로서의 사용가능성이 확인되었다. The in vitro regeneration and genetic transformation systems in hot pepper(Capsicum annuum L.) have not been routinely available, which has been a major limiting factor in the application of new genetic manipulations. An efficient procedure to regenerate whole pepper plants and to generate transgenic plants expressing a foreign gene was established. A relatively high frequency of plant regeneration was observed when hypocotyl and cotyledon explants were cultured on MS medium supplemented with NAA 0.1 mg/L plus zeatin 2.0 mg/L or IBA 10.0 mg/L plus BAP 1.0 mg/L. Addition of AgNO$_3$5 $\mu$M to these media improved the regeneration frequency up to 8%. For plant transformation, hypocotyl and cotyledon explants of hot pepper were precultured on shoot induction media without kanamycin added for 2 days, and then cocultured with Agrobacterium tumefaciens pDY183 for 2 days. Putative transformants were obtained from selection media containing 100 mg/L kanamycin sulfate and 500 mg/L carbenicillin. Putatively selected transformants were confirmed by amplification of selectable marker genes (ADA and NPT II) by polymerase chain reacion. Successful transcripts of ADA gene were detected by Northern blot analysis. Enzyme activity of ADA was also examined by spectrophotometric analysis, and expression of ADA gene in hot pepper suggests the potential application of ADA gene as a selectable marker in plants.

The Leaf Part of Capsicum annuum L. var. angulosum Mill Reduces the Hepatic Detoxification Capacity

Won Sup Lee,Mi-jin Seo,Soo-Young Kwak,Un-Joo Lee,Yong-Za Chung 대한암예방학회 2007 Journal of cancer prevention Vol.12 No.4

We previously demonstrated that the leaf part of Capsicum annuum L. var. angulosum Mill (leaf of green peppers, CA-L) has anti-tumor activity on the cancer cells. In this study, we evaluated the changes in hepatic enzymes especially relating to the detoxification for the evaluation of its effects on the liver, because it is recently reported that deficiency of hepatic enzymes relating to the detoxification can be a predisposing factor for the drug-induced hepatitis, and the other adverse clinical effects on rats and mice. We found that daily administration of the extract of CA-L (ECA-L) to the rats and mice for 2 weeks lead to decreases in glutathione and glutathione S-transferase levels of the liver in dose-dependent manners in rats and mice (p=0.029, p=0.022, respectively). In addition, the γ-GTP levels in the liver were increased 2 weeks after administration of ECA-L in rats and mice in dose-dependent manners. No other adverse clinical effects on rats and mice were found except on the liver. Our study suggests that 2-weeks administration of ECA-L to the rats and mice should decreases in hepatic detoxification capacity in dose-dependent manners. In this report, it can be assumed that more than 2 weeks administration of ECA-L should be a risk factor for drug-induced hepatitis, especially in combination with potential hepatotoxic drugs. (Cancer Prev Res 12, 287-295, 2007) KeyWords:Capsicum annuum L. var. angulosum Mill, Detoxification, Glutathione, Glutathione S- transferase, Hepatotoxicity, Leaf

고추 품질 관련 특성 연구를 위한 F6 RIL 집단의 원예적 특성 평가

김정호,조명철,조영일,이혜은,강원희,우종규,안율균 한국육종학회 2012 한국육종학회지 Vol.44 No.4

본 연구는 고추의 다양한 품질 관련 특성 연구를 위한 분자육종시스템 구축의 기초 연구로서, 고추의 매운맛과 고색소, 다양한 색소, 과형 등이 다양한 고세대(F6) RIL 집단을 육성하여 그 원예적 특성을 조사하였다. 자방친으로 단고추 계통인 “만다린(Capsicum annuum L.)”을 이용하였고, 화분친으로는 “블랙클러스터(Capsicum annuum L.)”를 이용하여 F1 조합을 작성한 후, 자식으로 세대를 유지한 고세대(F6) RIL 집단 129계통들에 대해서 과실특성과 초장, 잎, 꽃 등 13가지 원예적 특성들을 평가했다. 미숙과에서 성숙과로의 색 변화의 경우 그 변화 양상이 매우 다양하여 모두 12가지 양상으로 구분해 볼 수 있었는데, 녹색에서 바로 적색으로 변화하는 양상이 51계통으로 가장 많은 분포를 차지했고, 녹색에서 진보라색으로의 변화가 45계통, 상아색에서 주황색으로 변색하는 양상이8계통 등 숙기에 따른 과색 변화가 대단히 다양한 것을 확인할 수 있었다. 숙과색은 적색과가 99계통으로 가장 많은 분포를 보였고, 주황색이 17계통, 황색이 13계통이었다. 과형은 크게 세가지로 구분되었는데, 삼각형 과실이 64계통으로 가장 많은 분포를 보였고, 타원형이 52계통, 심장형이 13계통이었다. 착과방향은 61.2%가 상향 착과성을 보였고, 38.7%가 하향이었다. 원예적 특성 평가 결과 본 F6 RIL 집단은 그 다양성이 풍부하여 향후 고추 품질 특성 연구에 유용하게 활용될 수 있을 것으로 기대된다. Thirteen horticultural characteristics included fruit color, fruit shape, fruit-tip orientation and others were evaluated using an intra-specific population of 129 F6 recombinant inbred lines (RILs), which derived from a cross between ‘Mandarin’ (Capsicum annuum L.), large sized yellow bell pepper, and ‘Blackcluster’ (Capsicum annuum L.), small sized, deep purple fruit colored and highly pungency. There were 12 patterns of fruit color changed during fruit maturity. The most distribution of the pattern was from green to red observed in 51 RILs, the following pattern was green to purple and ivory to orange in 45 and 8 RILs, respectively. Red mature fruit color was observed in 99 RILs, orange color in 17 RILs, and yellow color in 13 RILs. The fruit shape was divided into three major groups. Triangular fruit distributed highest number of RILs (64 lines), the following was oval shape (52 lines) and cardioid shape (13 lines). Fruit-tip orientation was also examined and resulted that erect type was observed in 79 lines, and pendant type was in 50 lines. The presence of variability of characteristics in study population can be utilized for research of various fruit quality-related traits in the future.

Inhibitory effects of Capsicum annuum L. water extracts on lipoprotein lipase activity in 3T3-L1 cells

Jongmi Baek,Jaesung Lee,Kyoungkon Kim,Taewoo Kim,Daejung Kim,Cheonan Kim,Kanazawa Tsutomu,Sarangowa Ochir,Kooyeon Lee,Cheol Ho Park,Yong-Jik Lee,Myeon Choe 한국영양학회 2013 Nutrition Research and Practice Vol.7 No.2

Obesity, an intractable metabolic disease, currently has no medical treatment without side effects, so studies have been actively carried out to find natural compounds that have anti-obesity activity with minimum side effects. In this study, the anti-obesity effects of water extracts of seven Capsicum annuum L. varieties being Putgochu (Pca), Oyee gochu (Oca), Kwari putgochu (Kca), Green pepper (Gca), Yellow paprika (Yca), Red paprika (Rca) and Cheongyang gochu (Cca), were examined through the evaluation of lipoprotein lipase (LPL) mRNA expression level in 3T3-L1 cells (mouse pre-adipocytes). After capsaicin elimination by chloroform defatting, freeze-dried powder of Cca was treated to 3T3-L1 cells and anti-obesity effects were examined by determining the LPL mRNA level using the RT-PCR method. Of the primary fractions, only proven fractions underwent secondary and tertiary refractionating to determine anti-obesity effects. From seven different Capsicum annuum L., there was a significant decrease of the LPL mRNA expression level of 50.9% in Cca treatment compared to the control group. A significant decrease of the LPL mRNA expression level was shown in primary fractions (Fr) 5 (36.2% decrease) and 6 (30.5% decrease) of the Cca water extracts. Due to the impurities checked by UPLC chromatography, Fr 5 and 6 were refractionated to determine the LPL mRNA expression level. Treatment of Fr 6-6 (35.8% decrease) and Fr 5-6 (35.3% decrease) showed a significant decrease in the LPL mRNA expression level. When analyzed using UPLC, major compounds of Fr 6-6 and Fr 5-6 were very similar. Subsequently, we refractionated Fr 6-6 and Fr 5-6 to isolate the major peak for structure elucidation. Treatment of Fr 5-6-1 (26.6% decrease) and Fr 6-6-1 (29.7% decrease) showed a significant decrease in the LPL mRNA expression level. Consequently, the fractions may have a possibility to ameliorate obesity through the decrease of the LPL mRNA expression level.

자동화 HPLC/SPE/HPLC 시스템(Sepbox system)을 활용한 고추 잎 (leaf of Capsicum annuum L.) 추출물 분리 및 α-glucosidase 억제 활성 물질 탐색

김민선 ( Min-seon Kim ),진종범 ( Jong Beom Jin ),이정환 ( Jung Hwan Lee ),안혜숙 ( Hye Suck An ),판철호 ( Cheol-ho Pan ),박진수 ( Jin-soo Park ) 한국응용생명화학회(구 한국농화학회) 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.1

식물 유래 화합물은 다양한 생리활성과 구조 다양성으로 많은 연구자에게 관심을 받고 있으나 화합물 분리는 많은 시간 소요, 과량의 유기용매 소모와 분리과정 중의 활성성분의 유실 등의 어려움을 겪게 된다. 이러한 점을 극복하기 위하여 HPLC/SPE/HPLC를 결합한 Sepbox 분리시스템을 이용하여 고추잎 추출물에서 288개 분획물을 획득하였고 이 중에서 alpha-glucosidase 저해효능을 가지는 화합물인 luteolin 7-O-glucoside를 효과적으로 확인할 수 있었다. 또한 고추잎에 풍부한 플라보노이드 다당체를 가수분해함에 따라 해당 활성이 증가함을 검증하였다. 그러므로 본 연구결과는 Sepbox 시스템이 생리활성 평가와 결합할 경우 식물 내 유용물질 탐색에 효과적임을 보여주었다. Phytochemicals include plant-derived natural products that promote and improve the human metabolism and physiological activity, and there is a lot of research to find the value of the molecules is in progress. Likewise, we obtained 288 fractions of Capsicum annuum L. extract in less than 20 h using HPLC/SPE/ HPLC coupling experiment through Sepbox system, an effective separation system to search for active substances in natural resources and ensure efficacy and reliability. Therefore, this experiment allowed rapid identification of biologically active molecules from the extract compared to traditional separation processes. Of the above fractions, eight fractions showed the α- glucosidase inhibitory (AGI) activity and subsequent LC-MS analysis revealed one of the active molecules as luteolin 7-Oglucoside. In addition, we proved the increase in AGI activity according to deglycosylation of flavonoid glycoside. Therefore, this study suggests that the Sepbox system can quickly separate and identify active components from plant extract, and is an effective technique for finding new active substances.

Construction of a linkage Map in Capsicum annuum L. Using RAPD Markers and Identification of Two QTLs.

Yang, Tae-Jin,Kim, Yong-Jae,Park, Hyo-Guen The Korean Society of Plant Biotechnology 1999 Plant molecular biology and biotechnology research Vol.1 No.2

A linkage map of Capsicum annuum L. was constructed by random amplified polymorphic DNA (RAPD) markers followed in a backcross population of an intraspecific cross between cultivars HDA210 and Yatsufusa. A total of 420 random primers were tested and 311 polymorphic bands were generated by 158 random primers. Among them, 86 Yatsufusa specific bands generated by 52 primers were examined for mapping. Most bands except three segregated in Mendelian fashion fitting the expected 1:1 ratio. The total length of the map was 533 cM distributed in 15 linkage groups. The map distance between adjacent markers ranged 0 to 32.8 cM, with an average distance of 9.1 cM (63 markers). Some markers were clustered and this may be due to the amplification of a repetitive sequence by the RAPDs. Primer pairs for a sequence characterized amplified region (SCAR) were developed and the segregation scores by the SCAR primers were in accordance with the RAPD data. Two QTL markers for number of axillary shoots and for early flowering were developed. One QTL for early flowering located in the linkage group 3 and explained 61 "io of the phenotypic variation. The other QTL for the number of axillary shoots located in the linkage group 4 explained 55 % of the phenotypic variation.tion.

Fungal Elicitor와 Exo-phytohormone이 청양고추(Capsicum annuum L.) 종자발아와 2차 대사산물에 미치는 효과

진한솔 ‧ 전우성 ‧ 김성재 ‧ 맹대현 ‧ 박세익 ‧ 이인순 ‧ 박흠대 대구대학교 산업기술연구소 2018 産業技術硏究 Vol.29 No.2

We studied growth rate and synthesis of secondary metabolite as capsaicin and polyphenolic compound during seed germination of Capsicum annuum L. on condition media with fungal elicitor and exo-phytohormone. The condition media was consisted MS (Murashige & Skoog) medium and sucrose for carbon source with different concentration of elicitor and exo-phytohormone. The fungal elicitor was made by Aspergillus niger KCCM 11240 and exo-phytohormone used kinetin, IAA (indole-3-acetic acid). The secondary metabolites were extracted from the seeding tissue by acetone as solvent after 20 days. As a result, the germination of seed was found on the third day and growth of seeding was observed on the seventh day. The capsaicin concentration was had the highest concentration in R0’’ (no exo-phytohormone, 180 mg/ml of fungal elicitor) extract among all extract solutions. The polyphenolic compound was had high concentration in R1’’ (0.1 mg/L of IAA and kinetin, 180 mg/ml of fungal elicitor) extract. The inhibition rate of lipid peroxidation was showed in proportion to the concentration of capsaicin. The effect of NO radical scavenging was measured more activity to natural pepper fruit than seeding cultured in vitro and that activity considered to be phytonutrient included capsaicin and polyphenolic compound. The fungal elicitor inhibited growth and differentiation of seeding, but it induced the synthesis of secondary metabolites. The secondary metabolites were in inverse proportion to total length of seeding. Therefore, capsaicin as phytonutrient can mass-produce by plant tissue culture with biotic stress.