다중 덮개시스템의 모세관 방벽 효과 평가

이정환,조현진,정재열,정해룡,윤정현,Lee, Jeong-Hwan,Cho, Hyun-Jin,Cheong, Jae-Yeol,Jung, Haeryong,Yoon, Jeong Hyoun 한국지하수토양환경학회 2016 지하수토양환경 Vol.21 No.1

Capillary barriers, consisting of relatively fine-over-coarse materials, have been suggested as an alternative to traditional compacted soil covers. So, We were analysed to capillary barrier effect according to five cases of multi-layer cover systems. Water balance simulation was conducted with unsaturated flow model HELP to assess unsaturated hydraulic parameters such as hydraulic conductivity, climate affecting the performance of capillary barriers. Simulation were conducted for 5 Cases in the Ulsan area. Result of simulation indicated that three cases was formed unsaturated condition and capillary barrier effect.

Effect of Capillary Barrier on Soil Salinity and Corn Growth at Saemangeum Reclaimed Tidal Land

Sanghun Lee,Su-Hwan Lee,Hui-su Bae,Jang-Hee Lee,Yang-Yul Oh,Tae-Hwan Noh,Geon-Hwi Lee 한국토양비료학회 2014 한국토양비료학회지 Vol.47 No.6

Salt accumulation at soil surface is one of the most detrimental factors for crop production in reclaimed tidal land. This study was conducted to investigate the effect of capillary barriers beneath the soil surface on dynamics of soil salts at coarse-textured reclaimed tidal land. A field experiment was conducted at Saemangeum reclaimed tidal land for two years (2012-2013). Capillary barriers (3.5x12 m) were treated with crushed-stone, oyster shell waste, coal briquette ash, coal bottom ash, rice hull and woodchip at 40-60 cm depth from soil surface. Silage corn (Zea mays) was cultivated during the experimental period and soil salinity was monitored periodically. Soil salinity was significantly reduced with capillary barrier compared to that of control. Oyster shell waste was one of the most effective capillary barrier materials to control soil salinity at Saemangeum reclaimed tidal land. At the first growing season capillary barrier did not influence on corn growth regardless of types of the material, but plant biomass and withering rate of corn were significantly improved with capillary barrier at the second growing season. The results of this study showed that capillary barrier was effective on the control of soil salinity and improvement of corn growth, which indicated that capillary barrier treatment can be considered one of the best management practices for stable crop production at Saemangeum reclaimed tidal land.

Effect of Capillary Barrier on Soil Salinity and Corn Growth at Saemangeum Reclaimed Tidal Land

Lee, Sanghun,Lee, Su-Hwan,Bae, Hui-Su,Lee, Jang-Hee,Oh, Yang-Yul,Noh, Tae-Hwan,Lee, Geon-Hwi 한국토양비료학회 2014 한국토양비료학회지 Vol.47 No.6

Salt accumulation at soil surface is one of the most detrimental factors for crop production in reclaimed tidal land. This study was conducted to investigate the effect of capillary barriers beneath the soil surface on dynamics of soil salts at coarse-textured reclaimed tidal land. A field experiment was conducted at Saemangeum reclaimed tidal land for two years (2012-2013). Capillary barriers ($3.5{\times}12m$) were treated with crushed-stone, oyster shell waste, coal briquette ash, coal bottom ash, rice hull and woodchip at 40-60 cm depth from soil surface. Silage corn (Zea mays) was cultivated during the experimental period and soil salinity was monitored periodically. Soil salinity was significantly reduced with capillary barrier compared to that of control. Oyster shell waste was one of the most effective capillary barrier materials to control soil salinity at Saemangeum reclaimed tidal land. At the first growing season capillary barrier did not influence on corn growth regardless of types of the material, but plant biomass and withering rate of corn were significantly improved with capillary barrier at the second growing season. The results of this study showed that capillary barrier was effective on the control of soil salinity and improvement of corn growth, which indicated that capillary barrier treatment can be considered one of the best management practices for stable crop production at Saemangeum reclaimed tidal land.

Effect of Capillary Barrier on Soil Salinity and Corn Growth at Saemangeum Reclaimed Tidal Land

이상훈,이수환,배희수,이장희,오양율,이근휘,노태환 한국토양비료학회 2014 한국토양비료학회지 Vol.47 No.6

Salt accumulation at soil surface is one of the most detrimental factors for crop production in reclaimed tidalland. This study was conducted to investigate the effect of capillary barriers beneath the soil surface ondynamics of soil salts at coarse-textured reclaimed tidal land. A field experiment was conducted atSaemangeum reclaimed tidal land for two years (2012-2013). Capillary barriers (3.5x12 m) were treated withcrushed-stone, oyster shell waste, coal briquette ash, coal bottom ash, rice hull and woodchip at 40-60 cmdepth from soil surface. Silage corn (Zea mays) was cultivated during the experimental period and soil salinitywas monitored periodically. Soil salinity was significantly reduced with capillary barrier compared to that ofcontrol. Oyster shell waste was one of the most effective capillary barrier materials to control soil salinity atSaemangeum reclaimed tidal land. At the first growing season capillary barrier did not influence on corngrowth regardless of types of the material, but plant biomass and withering rate of corn were significantlyimproved with capillary barrier at the second growing season. The results of this study showed that capillarybarrier was effective on the control of soil salinity and improvement of corn growth, which indicated thatcapillary barrier treatment can be considered one of the best management practices for stable crop productionat Saemangeum reclaimed tidal land.

LPS에 의한 급성 폐손상에서 게르마늄에 의한 폐포-모세혈관 장벽의 변화

이윤정,조현국,신건호,전경희,Lee, Yoon-Jeong,Cho, Hyun-Gug,Sin, Gun-Ho,Jeune, Kyung-Hee 한국현미경학회 2009 Applied microscopy Vol.39 No.1

급성호흡곤란증후군은 다양한 원인에 의해 발생하는 급성 염증성 폐질환으로써 폐포-모세혈관 장벽의 파괴로부터 기인하며 파괴 기전에 호중구가 중요한 역할을 담당한다. 이에 대한 게르마늄의 항염증 효과 즉, 호중구의 폐조직 내 유주 억제로 인한 폐포-모세혈관의 손상의 감소로 폐손상이 경감되는지를 확인하고자 하였다. 실험군은 생리식염수를 투여한 대조군(CON), 내독소 5시간 투여군(LPS), 그리고 게르마늄 1시간 전처리 후 내독소 5시간 투여군(Ge+LPS)으로 나누었으며, 내독소는 $100{\mu}g$을 0.5 mL 생리식염수에 녹여 기도내로 분무하고, 게르마늄은 체중 100 g당 26 mg을 복강으로 투여하였다. 내독소의 주입으로 급성 폐손상을 유도시킨 내독소 투여군에서는 대조군과 비교하여 폐부종(p<0.001), 기관지폐세척액 내 단백질 함량(p<0.05), 호중구의 침윤(p<0.001)이 현저히 증가하였으나 게르마늄을 전처리 한 군은 내독소 투여군과 비교하여 모든 항목에서 유의하게 감소하였다. 현미경을 통한 관찰 결과에서도 게르마늄 전처리군은 내독소 투여군에 비해 내피세포, 제I형 폐포세포, 기저판으로 구성된 폐포-혈관장벽의 구조가 비교적 잘 보존되었고, 제II형 폐포세포의 Lamellar body와 미세융모 그리고 기저판의 구조도 비교적 정상적으로 보존된 상태로 나타났다. 그러므로 게르마늄은 항염증물질로 작용, 즉 호중구의 폐조직 내로의 유주현상을 방해하여 활성화된 호중구의 독성물질로 인한 폐포-모세혈관의 손상을 감소시킬 수 있었으므로 결과적으로 내독소로 유도된 폐손상은 게르마늄의 전처리에 의해 억제되었다고 판단된다. Acute respiratory distress syndrome (ARDS), also known as an acute inflammatory lung disease is developed by various factors that is originated from the destruction of alveolar-capillary barrier, and neutrophils plays an important role in the destruction. The study intended to confirm, the anti-inflammatory effect of germanium, whether a lung injury has been mitigated with the reduction of injury in alveolar-capillary barrier resulting from inhibition of neutrophils migration in lung tissue. Test groups were divided in saline administered CON, 5 hours of endotoxin administered LPS and 5 hours of endotoxin administered Ge+LPS following 1 hours of pre-processed germanium. $100{\mu}g$ endotoxin was melted in 0.5 mL saline and sprayed into airway and 26 mg germanium per 100 g weight was administered into abdominal cavity. The endotoxin group which induced an acute lung injury with administered endotoxin showed dramatic increase of pulmonary edema (p<0.001), protein contents in bronchoalveolar lavage fluid, BALF (p<0.05) and neutrophils of infiltration in BALF (p<0.001) comparing with a control group, while a pre-treated germanium group showed significant decrease in all categories comparing to the endotoxin administerd group. In the result of a microscopic observation, the structure of alveolar-capillary barrier which is constructed with basal lamina, alveolar type I cells and endothelial cell were preserved of the pre-treated germanium group relatively well compare to the endotoxin administered group. And the construction of lamellar body, microvilli and basal lamina of alveolar type II cells were also preserved relatively well. Hence, germanium activates as an anti-Inflammatory mediator in other words, it interfered neutrophils migration into lung tissue, thereby reduced injury of alveolar-capillary barrier from toxic substances of activated neutrophils. Consequently, the study has determined that the acute lung injury induced by endotoxin has been decreased by the pre-treated germanium.

토양 모세관상승 분석을 통한 염분차단층 비교실험 연구 – 영종도 간척지 중심으로 –

이정규,이시영 전북대학교 부설 휴양및경관계획연구소 2023 휴양및경관연구 (J East Asian Landscape Studies) Vol.17 No.2

A recent golf course considers an ecosystem and environment by location diversification therefore it builds up a reclaimed land of littoral. This study is for helping to grow economical and normal trees and plants in a long-term by planning of growing trees and plants from the first of business. We researched for water drainage system of the A golf course in Yong Jong Do to improve the soil environment. It preceded a catabolism study of a reclaimed land of littoral soil environment. This study will analyze natural obstacles for building up a golf course and investigate important point for improving and be a guide to the economical construction with reducing a trial and error. It analyzes that a salt rising or a salt excess of ground affect directly to grow a plant in a reclaimed land of littoral. Therefore, it needs to establish the interception layer and improve soil through desalting. Especially, it is effective for the interception of capillary rising that the bigger crop material and thicker interception layers. This study is worth deciding a material selection and interception layer thickness by various reclamation methods. However, the setting of the composition thickness of the planting cross-section layer was not sufficient due to the limitations of the indoor test, and especially in the case of the southwest coast, factors that have environmental effects (tidal level difference between high and low tide, sunlight, wind direction, air volume, etc.) Although the limitations of non-application of variable conditions for and the increase in salinity can be expected when the salt barrier layer is installed, countermeasures against drying damage due to blocking of the parent water supply were lacking in the research process. Research on the lacking part in the future should be continued continuously in the future. 본 연구는 임해 매립지에 조성되는 골프장 부지의 자연적 장해요인을 분석하고 개선 요소를 검토하 여 향후 매립지에 조성되는 식재공사 등의 시행착오를 줄이고 경제성을 향상시키고자 한다. 선행 연 구자들의 임해 매립지 토양 환경의 이화학적 연구 결과를 토대로 영종도 국제공항 내 골프장의 토양 환경을 개선하는 방법을 배수 처리 체계와 함께 연구하였다. 그 결과로 사업 초기 단계에서부터 수목 또는 식물의 생장을 고려한 계획을 통해 경제적이며, 장기적으로 정상적인 식물 또는 수목의 생장에 도움을 꾀하고자 하였다. 연구결과로서 임해 매립지에서 식물 생육에 영향을 주는 요인 중 염분 상승 또는 식생 지반의 염분 과다는 식물 생육에 직접적인 영향이 있는 것으로 분석되었고 따라서 임해 매립지의 경우 탈염 과정을 거쳐 식물 생육에 적합한 토양으로 개선하여야 될 필요가 있으며, 이 과정 에서 염분 상승의 차단을 위한 차단층의 설치가 필요하다. 특히 모세관 상승의 차단은 입도가 큰 재료 일수록 차단 효과가 컸으며, 실험 결과를 미루어 볼 때 차단층의 두께가 클수록 확실한 모세관상승 차단 효과가 있을 것이라 판단된다. 본 연구는 임해 매립지 조성 시 염분 상승에 의한 식물 생육 저해 요소를 감소시키기 위한 방법으로 식재기반 조성 시 매립 방법을 달리하여 지하수위를 저하 시킴으로써 모세관상승에 의한 염분 상승을 근본적으로 차단하고자 실험적인 방법을 통해 차단층의 재료선정 및 차단층의 두께를 결정하는데 연구의 의의를 두고 있다. 그러나 식재 단면층의 조성 두께 등의 설정은 실내시험으로 인한 한계를 보이고 있다. 특히 서남해안의 경우 환경적 영향(밀물과 썰물의조위 차, 일조량, 풍향, 풍량 등 염분 상승)이 되는 인자들의 변수 조건에 대한 미적용의 한계성과 염분차단층 설치 시 염분 상승은 기대할 수 있으나 모관수 차단으로 인한 건조피해에 대한 대책은 연구과정에서 부족했던 부분이다 추후 부족한 부분에 대한 연구는 향후 지속적으로 계속되어야 할 것이다.

Nitrone계 항산화제 (PBN)의 뇌에서 혈액으로의 배출과 뇌 수송 특성

이나영,강영숙 대한약학회 2003 약학회지 Vol.47 No.4

We have investigated the transport characteristics of synthetic antioxidant and free radical scavenger, $\alpha$-phenyl-n-tert-butyl nitrone (PBN) at the blood-brain barrier (BBB) by in vitro uptake study in conditionally immortalized rat brain capillary endothelial cell line (TR-BBB). Also, the efflux of PBN from brain to blood is estimated using the brain efflux index (BEI) method. Choline is a charged organic cation, including nitrogen-methyl group and shows the carrier-mediated distribution to the brain. [$^3$H]Choline uptake by TR-BBB cells was significantly inhibited by PBN with $IC_{50}$/ of 1.2 mM, which appears to be due to similar structures between choline and PBN. And, PBN was microinjected into Par2 of the rat brain by BEI method, and was eliminated from the brain with an apparent elimination half-life of about 2 min. Also, [$^3$H]choline efflux was significantly inhibited by PBN using BEI method. In conclusion, the efflux transport of PBN takes place across the BBB and PBN may be transported into the brain and eliminated from the brain by BBB choline transporter.

Effects of Bisphosphonates on Glucose Transport in a Conditionally Immortalized Rat Retinal Capillary Endothelial Cell Line (TR-iBRB Cells)

( Na Young Lee ),( Hyun Joo Park ),( Young Sook Kang ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.1

The objective of the present study was to elucidate the effect of bisphosphonates, anti-osteoporosis agents, on glucose uptake in retinal capillary endothelial cells under normal and high glucose conditions. The change of glucose uptake by pre-treatment of bisphosphonates at the inner blood-retinal barrier (iBRB) was determined by measuring cellular uptake of [3H]3-O-methyl glucose (3-OMG) using a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB cells) under normal and high glucose conditions. [3H]3-OMG uptake was inhibited by simultaneous treatment of unlabeled D-glucose and 3-OMG as well as glucose transport inhibitor, cytochalasin B. On the other hand, simultaneous treatment of alendronate or pamidronate had no significant inhibitory effect on [3H]3-OMG uptake by TR-iBRB cells. Under high glucose condition of TR-iBRB cells, [3H]3-OMG uptake was increased at 48 h. However, [3H]3-OMG uptake was decreased significantly by pre-treatment of alendronate or pamidronate compared with the values for normal and high glucose conditions. Moreover, geranylgeraniol (GGOH), a mevalonate pathway intermediate, increased the uptake of [3H]3-OMG reduced by bisphosphonates pre-treatment. But, pre-treatment of histamine did not show significant inhibition of [3H]3-OMG uptake. The glucose uptake may be down regulated by inhibiting the mevalonate pathway with pre-treatment of bisphosphonates in TR-iBRB cells at high glucose condition.

Effects of Bisphosphonates on Glucose Transport in a Conditionally Immortalized Rat Retinal Capillary Endothelial Cell Line (TR-iBRB Cells)

이나영,박현주,강영숙 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.1

The objective of the present study was to elucidate the effect of bisphosphonates, anti-osteoporosis agents, on glucose uptake in retinal capillary endothelial cells under normal and high glucose conditions. The change of glucose uptake by pre-treatment of bisphosphonates at the inner blood-retinal barrier (iBRB) was determined by measuring cellular uptake of [3H]3-O-methyl glucose (3-OMG) using a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB cells) under normal and high glucose conditions. [3H]3-OMG uptake was inhibited by simultaneous treatment of unlabeled D-glucose and 3-OMG as well as glucose transport inhibitor, cytochalasin B. On the other hand, simultaneous treatment of alendronate or pamidronate had no significant inhibitory effect on [3H]3-OMG uptake by TR-iBRB cells. Under high glucose condition of TR-iBRB cells, [3H]3-OMG uptake was increased at 48 h. However, [3H]3-OMG uptake was decreased significantly by pre-treatment of alendronate or pamidronate compared with the values for normal and high glucose conditions. Moreover, geranylgeraniol (GGOH), a mevalonate pathway intermediate, increased the uptake of [3H]3-OMG reduced by bisphosphonates pre-treatment. But, pre-treatment of histamine did not show significant inhibition of [3H]3-OMG uptake. The glucose uptake may be down regulated by inhibiting the mevalonate pathway with pre-treatment of bisphosphonates in TR-iBRB cells at high glucose condition.

Effects of Bisphosphonates on Glucose Transport in a Conditionally Immortalized Rat Retinal Capillary Endothelial Cell Line (TR-iBRB Cells)

Lee, Na-Young,Park, Hyun-Joo,Kang, Young-Sook The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.1

The objective of the present study was to elucidate the effect of bisphosphonates, anti-osteoporosis agents, on glucose uptake in retinal capillary endothelial cells under normal and high glucose conditions. The change of glucose uptake by pre-treatment of bisphosphonates at the inner blood-retinal barrier (iBRB) was determined by measuring cellular uptake of $[^3H]3$-O-methyl glucose (3-OMG) using a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB cells) under normal and high glucose conditions. $[^3H]3$-OMG uptake was inhibited by simultaneous treatment of unlabeled D-glucose and 3-OMG as well as glucose transport inhibitor, cytochalasin B. On the other hand, simultaneous treatment of alendronate or pamidronate had no significant inhibitory effect on $[^3H]3$-OMG uptake by TR-iBRB cells. Under high glucose condition of TR-iBRB cells, $[^3H]3$-OMG uptake was increased at 48 h. However, $[^3H]3$-OMG uptake was decreased significantly by pre-treatment of alendronate or pamidronate compared with the values for normal and high glucose conditions. Moreover, geranylgeraniol (GGOH), a mevalonate pathway intermediate, increased the uptake of $[^3H]3$-OMG reduced by bisphosphonates pre-treatment. But, pre-treatment of histamine did not show significant inhibition of $[^3H]3$-OMG uptake. The glucose uptake may be down regulated by inhibiting the mevalonate pathway with pre-treatment of bisphosphonates in TR-iBRB cells at high glucose condition.