Chiral Separation of (±)-Higenamine by Capillary Electophoresis

Choi,One-Kyun,Jung,Kyo-Soon,Choi,Heisook-Yun,Yang,Deok-Chun 한국자원식물학회 2003 Plant Resources Vol.6 No.1

Higenamine [1-(4-hydroxy-6, 7-dihydroxy-l, 2, 3, 4-tetrahydroisoquinoline) is a cardiotonic constituent of Aconiti tuber, one of the most widely prescribed oriental medicines. S-(-)higenamine was reported to have a stronger cardiotonic activity than R-(+)-higenamine and known as a central intermediate in the biosynthesis of various benzyl isoquionoline alkaloids in plants. The separation of higenamine enantiomers has been accomplished with capillary electrophoresis using cyclodextrins (CDs) as chiral selectors. Good resolution of this enantiomers was obtained using a 50 mM sodium phosphate buffer containing hydroxypropyl β-CDs using 27 cm fused silica capillary (50㎛ i.d., 20 cm to detector) at 25℃. With the electric field of 340 V/cm, the separation time of higenamine enantiomers was less than 6 min. Under this optimum conditions, the relative standard deviations of migration time and peak area were less than 1.6% and 3.2%. A 512-channel diode array detector was confirmed for the higenamine. The detection limits (S/N = 3) of these enantiomers are 1.5mutextrmm/mL. We confirmed the chiral form of higenamine in medicinal plants.

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 - Ⅴ. 過去의 健康疾病 및 苦痛에 대한 態度 -

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),임채환(Chae-Hwan Rhim),양종순(Jong-Soon Yang),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeong-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choe),변대석(Dae-Seok Byun),김무남(Mu-Nam 군산대학교 수산과학연구소 1994 군산대학교 수산과학연구소 연구논문집 Vol.1 No.-

The present study was designed to be link a part of the research on the investigation on daily life and consciousness of the longevous people in Korea, and to investigate the health conditions, and the attitude on disease and suffering of longevous people in the past. 1. By the health conditions of longevous people between 40 and 60 years, ""very good"" showed the highest figure of 71.0%, followed by ""ordinary"" (21.6%). Consequently it is found that 92.6% of total longevous people surveyed maintained the good health conditions above ordinary. 2. Of the experiences of disease and/or injury after 60 years of longevous people, ""never"" showed the highest figure of 74.7%, while ""had"" showed the only 22.6% of total longevous people surveyed. 3. Of the details of disease and/or injury after 60 years of longevous people. ""digestive disease"" showed the highest figure of 32.6%. followed by ""apoplexia cerebri & hypertensions""(14.0%) and ""heart disease""(14.0%), and followed by ""respiratory disease""(12.3%). 4. Of the experiences of suffering between 40 and 60 years of longevous people, ""never"" showed 53.7% of total longevous people surveyed, while the frequency of suffering decreased in the order of ""once""(22.7%), ""2 times""(15.8%) and ""3 times""(4.7%). By the details of suffering between 40 and 60 years of longevous people. ""family life"" showed the highest figure of 85.1%, followed by ""business""(10.1%). 5. By the attitude on suffering of longevous people, ""tried to forget soon"" showed the highest figure of 54.2%, followed by ""continuous worried""(23.3%), and followed by ""not worried""(7.2%).

8년간(1992~1999) 산업안전보건연구원에 의뢰된 직업병 심의 사례 분석

강성규,김규상,김양호,최정근,안연순,진영우,최병순,양정선,김은아,채창호,최용휴,김대성,박정선,정호근 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : Pneumoconisis and noise-induced hearing loss (NIHL) have been reported as main occupational diseases by the Special Health Examination. The Industrial Accident Compensation Insurance has reported various work-related diseases, however, these two diseases occupied almost a half of compensated cases. Therefore, it was not well known about the status of occupational diseases other than pneumoconiosis, NIHL, and cardio-cerebrovascular accident (CVA). This study was conducted to analyze claimed cases as an occupational disease, that was requested to the Korea Industrial Safety and Health Agency (KOSHA). Methods : The local office of the Korea Labor Welfare Corporation (KLWC) has asked the KOSHA for confirmation of claimed cases as an occupational disease. We analyzed 379 cases requested from KLWC, the Ministry of Labor, employers, unions and occupational health agencies from 1992 to 1999. Results : Male was 80.7 % of the requested cases. Their mean age was 42 years old and 75.5 % of them were more than 35 years old. The requested cases were increased rapidly from 25 cases in 1992 to 108 cases in 1999 and the accept rate was 50.7 %. The majority of requested cases were respiratory diseases(22.4%), cancers(18.5%), Neuropsychiatric problems(14.5%), and musculoskeletal problems(13.5%). The accept rate was high in reproductive, respiratory, musculoskeletal and digestive disorders and low in neuropsychiatric, renal and otologic problems and occupational cancers. 73.6% of them were caused by chemical agents, especially 28.5 % were by organic solvents. 67% of them were clinically confirmed at university hospitals. A half of the cases were from KyongIn area, even the request came from the whole country. Conclusions : A claim was common in workers whose age was over 35 years old and exposure history was over 10 years. The respiratory diseases and neuropsychiatric disorders were still main problems in occupational health and occupational cancers was increasing even though its accept rate was not high yet.

韓國人 靑少年의 身長과 體重의 成長過程에 따른 形態的 變化에 關한 硏究

崔重明,朴良元,朴淳永 慶熙大學校 1989 論文集 Vol.18 No.-

The purpose of this study is to identify the physical growth in Korean. The subjects of this study include both of male and female, who are attending elementary school, middle school, high school, college in middle and large cities and rural areas. The total number of subjects was 144, 583 persons, including 76,402 persons of male and 68,181 persons of female. The researcher measured the physical status of the male and female - in terms of body height and body weight - through cross - sectional method. On the basis of the results, calculated the standard values of each body structure, the physical and nutritional indices, fatness and estimate the standard body weight, under - and over - weight, the weight of malnutrion and obesity in accordance with age, sex and body height for decision of health level. These surveys and measurements took for 18 months from 1 May, 1988 to 30 April, 1989, and the data from these surveys were analyzed through computer. The results are as follows. 1. Body Measurements 1) Body height (㎝) In the growth condition of body height, rapid growth has been observed among male in the ages 7-15 and among female 7-13. Growth in terms of body height turned out to be slower among higher ages by both sexes, and the average body height of full-grown Korean youth (20-24 age group) was 170.6±7.1㎝ for male and 159.9±6.3㎝ for female. The maximum growth age and annual increment of body height were 10-11 years old and 6.73㎝ per year for female and 11-12 years old and 6.8㎝ for male. These data show a fact thai the female starts to grow earlier than the male by about one year. The age of crossover between the sexes come around 9.5-11.5 years of age, where upon female outgrows male. 2) Body weight(㎏) Rapid growth. in terms of body weight, which was represented by a straight line on a growth curve was observed among the both sexes of 7-15 years of ages and growth turned out to be slower among higher ages by both sexed. The average body weight of full - grown Korean youth (20 - 24 age group) was 63.8±6.8㎏ for male and 52.95±6.0㎏ for female. The maximum growth age and annual increment of body weight were 12 - 13 years old and 6.0㎏ per year for male and 10-11 years old and 5.6㎏ for female. Those data show a fact that the female starts to grow earlier than the male by about two year. The age of crossover between the sexes come around 9.5-12.5 years of age, whereupon outgrows male. 2. Physical and Nutritional Indices 1) Relative body weight Rapid growth, in terms of relative body weight, which was represented by a straight line on a growth curve was observed among the both sexes of 7 - 16 years of age and the age of crossover between the sexes come around 7 - 12.5 years of age. 2) Rohrer index Rohrer index displayed more rich value in case of female than male and in the adolescent period, the level of fullness in lower than after the completion of development. 3) Kaup index In both sexes, Kaup index increased in accordance with advancing ages. The index comes to under 2.02 from 7 to 14 years old in case of male and from 7 to 13 years old in case of female. So the growth of longitudinal axis seemed to be more poor than the one of vertical axis. The index increases beyond 2.0 in case of male of after 15 years old for female. 3. The Fat Amount Through the Method of Measurement of Physical Body In 7 years of age group, the amount of body fat of male was 3.77±1.25 ㎏ and that of female was 9.49±2.24㎏. The amount in creased with age to reach for male in 20-24 age group an average 11.3±2.81㎏ and for female 14.81±2.12㎏. In 7 years of age group, the body fat ( % ) of male was 15.35±5.14% and that of female was 18.01±2.87%. The body fat ( % ) increased with age to reach for male in 20-24 age group an average 17.63±2.67 % and 24.85±4.47 % for female. In cases of male, the body fat ( % ) of 8-13 age group indicated normal range but the other age group indicated higher range. In cases of female, the body fat (%) of below 10 age group indicated lower range and over 11 age group indicates normal range. 4. The Standard Body Weight of the Koreans 1) The standard body weight of Korean youth In case of 7 years age group, correlation coefficient of r=+0.572(p 0.001) between body height and body weight was found in a male group of 2,358 subjects and r=+0. 353(p 0.001) was found in female group of 2,461 subjects, from which respective linear regression equation of body weight and body height was established for male and female as follow : For male ; Y ( B. W, ㎏ )=0.4059×( B. H, ㎝ )-26.1, ( Sy. x =±3.7) female ; Y ( B. W, ㎏ )=0.1471×( B. H, ㎝)+6.3, (Sy. x=±5.4) Similar regression equations of body weight and height were found all age groups from 7 to 19 years old and listed in table 12. 2) Standard body weight of Koreans A correlation coefficient of r=+0.320( p<0. 001 ) between body height and body wight was found in a 20-24 age male group of 7,659 subjects and r=+0.301( p<0.001) in a 20 - 24 female group of 6,693 subjects, from which respective linear regression equation of body weight and height was established for male and female as follows : For male ; Y ( B. W. ㎏ )=0.306×( B. H. ㎝ )+11.6, ( Sy. x =±6.4) female ; Y ( B. W. ㎏ )=0.2856×( B. H, ㎝)+7.3, (Sy. x=±5.7) The standard body weight, under - and over - weight, weight of malnutrition and obestiy of Koreans calculated and listed in table 13-a and 13-b.

송곳니구름버섯(Irpex zonatus) BN2에 의한 다핵방향족 탄화수소화합물의 분해

최양순,윤경하 순천향대학교 기초과학연구소 1996 순천향자연과학연구 논문집 Vol.2 No.2

우리 나라 자연 환경에서 분리 동정된 송곳니구름버섯 (Irpex zonatus) BN2 균주의 리그닌 분해효소활성과 anthracene, phenanthrene 및 benzo(a)pyrene의 분해능을 조사하였다. 송곳니구름버섯 BN2 균주는 lignin peroxidase (LiP) 와 veratryl alcohol oxidase (VAO)를 생산하지 않고 laccase와 manganese dependent peroxidase (MnP)를 생산했다. MnP는 배양 3일부터 생산되었으나 효소활성은 매우 낮았다. 반면 laccase는 배양 초부터 지속적으로 생산되었고 활성은 대단히 높았다. 균주를 PAHs 화합물에 접종하여 배양했을 때 anthracene 분해율은 배양 1주에서 74.6%, 2주에서 92.9%, 4주에서 100%의 분해율을 접종하여 나타났다. phenanthrene 의 경우 배양 1주와 2주에서 각각 74.3%와 91.6%의 분해율을 나타냈고 4주에서 100%의 분해율을 나타냈다. benzo(a)pyrene 은 각각 41.8%, 49.0% 및 82.5%의 분해율을 나타내서 다른 PAHs 화합물보다 비교적 낮은 분해율을 보였다. 본 실험 결과 송곳니구름버섯 BN2 균주에 의한 PAHs 화합물의 분해는 주로 laccase에 의하여 이루어진다고 생각된다. The present research was undertaken to investigate the activity of ligninolytic enzymes and the biodegradation capability of polycyclic aromatic hydrocarbons (PAHs) with Irpex zonatus BN2, isolated from nature and identified. For the assay of enzyme activities, the isolate did not produce lignin peroxidase (LiP) and veratryl alcohol oxidase (VAO), but laccase and manganese dependent peroxidase (MnP). While the activity for MnP was low (61.6 n㏖/㎎·protein). its laccase activity was very high (1185.9 n㏖/㎎·protein). Moreover. laccase had appeared earlier than MnP When the isolate was incubated with 10㎎/L (final concentration) of anthracene, phenanthrene and benzo(a)pyrene, the isolate removed anthracene 74.6%, 92.9%, and 100% respectively for 7,14 and 28 days incubation. In case of phenanthrene was 74.3%, 91.6% and 100% respectively. Finally the results of benzo(a)pyrene was 41.8%, 49.0% and 82.5%, respectively. The results suggest that laccase of Irpex zonatus BN2 should play an important role in the biodegradation of these PAHs compounds.

자동차 도아프레임 오목부분의 아연도금법 개발

최순돈,민봉기,양승봉,신현준 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.1

We studied the homogeneous plating on the dented part of door frame by decreasing or increasing the current density, installing air blower, adjusting the length between anode and cathode, using auxiliary anode, developing the new plating bath and etc. When we performed the zinc cynade plating, there was no way to achieve the homogeneous plating layer without using auxiliary anode. However, it wasn't a suitable and efficient method for appling to the real process. After we developed the plating bath that had the uniform current density, we were able to get the homogeneous plating layer of zinc on the dented door frame.

韓國, 自由中國, 日本人 初中高校生의 體格과 營養狀態 比較에 關한 硏究

崔重明,朴良元,朴淳永 慶熙大學校 1989 論文集 Vol.18 No.-

On the basis of the presented data which look boy and girl students as their subjects in Korea. Japan and Republic of China in 1987, the author intended to compare and analyze the physique-growth status by age and sex. For that proposal, I had investigated the physical growths(body height, body weight, chest-girth and sitting height) of the three nations students from Aug. 26 in 1987 to Aug. 31. 1988 with the application of the medians of Korea. Republic of China and Japan. The results are as follows. A. Physical growth and development 1. Body height (㎝) In case of male students, Korean students ate superior in their body heights to Japanese students when they are before the year 14, but from 14 to 20 both of nations students are same. And then student of Republic of China are all inferior lo these of Korea and Japan through all these ages. In case of girl students, Koreans are taller than Japanese and Republic of China through all these ages. Chinese arc inferior in their heights to Koreans and Japanese until before the year 15. but same after that age. The interchange ages of the body height occured in the both sexes of 9.5-11.5 in Korean, 95-12.5 in Republic of China and 9.5-12.5 in Japan. During this period, girls are latter than boys, but after this period boys are taller than girls. Maximum growth age per year for male in Korea are the age 11-12(6.3㎝) and those for female are the age 10-11(6.73㎝): of Korean students, MGA appears one and two year earlier than that of Chinese and Japan. 2. Body weight (Kg) In case of male, Korean students show similar growing degrees through all the ages, and the students of Chinese and Japan indicate a little lower degree than Korean, In case of female, the similar phenomena are showed. The interchange ages of the body weight occured in the both sexes of 9.5-12.5 in Korean. 10.5-13.5 in Republic of China, and 10.0-13.5 in Japan. The MGA per year for male is the ages 12-13(5.45㎏) in Korea, 13-14 (5.46㎏) in Republic of China, and 13-14(6.5㎏) in Japan, while for female is the ages 10-11(5.07㎏) in Korea. 10-11 and 11-12(4.39㎏) in Republic of China, and 12-13(5.3㎏) in Japan, respectively. 3. Chest-girth (㎝) In case of both sexes, Korean students are superior of the chest-grith than Republic of China and Japan. The interchange ages of the chest-girth occured in the both sexes of 9.5-13.5 in Korea, 11.5-14.5 in Republic China and 10.5-13.5 in Japan. The MGA per year for male is the ages of 12-13(4.06㎝) in Korea, 13-14(4.7㎝) in Republic of China, and 13-14(3.77㎝) in Japan. While for female is the ages 10-11(4.58㎝), 12-13(3.92㎝), and 12-13(3.6㎝), respectively. The MGA of Korean male students appers one yeat earlier than thai of Chinese and Japanese, while for Korean female two years earlier than that of Chinese and Japanese. 4. Sitting height (㎝) Korean students are superior in their sitting height to Japanese student when they are before the year 15 in male and 16 in female; but after the year, Japanese students are superior. The interchange ages of the sitting height occured in the both sexes of 9.5-12.5 in Korean and 9.5-13.5 in Japan. B. Maximum growth age (M. G. A) Comparison growth age of Korean, Republic n| China and Japanese students are as follows.

ZMP를 이용한 굴삭기의 안정성에 관한 연구

최종환,엄혁,임태형,김승수,양순용,이병룡,안경관 한국공작기계학회 2003 한국공작기계학회 춘계학술대회논문집 Vol.2003 No.-

The hydraulic excavator has been a popular research object for automation because of its multi-workings and economic efficiency. When it works crane tasks, most of disasters happen. The stability of the excavator having crane function has a close relation with excavator's posture, motion and load. In this paper, the stability of tipping-over has been analysed using Zero Moment Point(ZMP),

송곳니구름버섯(Irpex zonatus)에 의한 Poly R-478 염료 탈색

최양순 서강정보대학 1996 論文集 Vol.15 No.-

This research was undertaken to investigate the most suitable source of carbon and nitrogen by white-rot fungi isolated from nature, in which process of decolorization of Poly R-478 dye, as polymeric dyes. 1. The isolates ware identified as Irpex zonatus 2. Glucose and urea were the most effective carbon and nitrogen sources and its optimal concentration were 1.0% and 0.04%, respectively 3. Under optimal conditions, decolorization of Poly R-478 dye for isolate was shown 88.4% after 7 days, and 93.4% after 10 days of incubation 4. In the presence of nitrogen source, the isolate showed the decolorization of dye, and rapidly increased decolorization when nitrogen source was depleted.

겔 확산법에 의한 장내세균과의 약제 감수성 검사법의 비교

최양순 서강정보대학 1992 論文集 Vol.11 No.-

Agar diffusion method is most widely used procedures for antimicrobial susceptibility test in clinical laboratory recently. So author made investigation into this method for antimicrobial susceptibility patterns of bacteria, that are isolated from clinical materials, and compared with results of susceptible patterns according to incubation times. In direct method, the inhibitory zone of 24 hours' exposed bacteria are reduced remarkably than those of 48hrs', 72hrs' and 96hrs'. 48hrs exposed bacteria's inhibitory zone are the same as those of over 48hrs' exposed bacteria. In indirect method, patterns of inhibitory zone resulted in regular, enrichmented in broth which inoculated in decline phase of bacteria, and showed different results between direct and indirect method.