A Rapidly New-typed Detection of Norovirus Based on F0F1-ATPase Molecular Motor Biosensor

Zhuo Zhao,Jie Zhang,Mei-Ling Xu,Zhi-Peng Liu,Hua Wang,Ming Liu,Yan-Yan Yu,Li Sun,Hui Zhang,Hai-Yan Wu 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.1

In order to adapt port rapid detection of food borne norovirus, presently we developed a new typed detection method based on F0F1-ATPase molecular motor biosensor. A specific probe was encompassed the conservative region of norovirus and F0F1-ATPase within chromatophore was constructed as a molecular motor biosensor through the “ε-subunit antibody-streptomycinbiotin- probe” system. Norovirus was captured based on probe-RNA specific binding. Our results demonstrated that the Limit of Quantification (LOQ) is 0.005 ng/mL for NV RNA and also demonstrated that this method possesses specificity and none cross-reaction for food borne virus. What’s more, the experiment used this method could be accomplished in 1 h. We detected 10 samples by using this method and the results were consistent with RT-PCR results. Overall, based on F0F1-ATPase molecular motors biosensor system we firstly established a new typed detection method for norovirus detection and demonstrated that this method is sensitive and specific and can be used in the rapid detection for food borne virus.

Protective Effects of Verapamil against H2O2-Induced Apoptosis in Human Lens Epithelial Cells

( Zhuo Wang ),( Dan Wang ),( Yan Li ),( Xiuli Zhang ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.6

Verapamil is used in the treatment of hypertension, angina pectoris, and atrial fibrillation. Recently, several studies have demonstrated that verapamil increased the optic nerve head blood flow and improved the retrobulbar circulation. All these show that verapamil is potentially useful for ophthalmic treatment. Thus, the aim of this study is to investigate whether verapamil could protect human lens epithelial cell (HLEC) from oxidative stress induced by H2O2 and the cellular mechanism underlying this protective function. The viability of HLEC was determined by the MTT assay and apoptotic cell death was analyzed by Hoechst 33258 staining. Moreover, Caspase-3 expression was detected by immunocytochemistry and flow cytometry analysis. We also detected Caspase-3 mRNA expression by reverse-transcription-polymerase chain reaction and the GSH content in cell culture. The results showed that oxidative stress produced significant cell apoptotic death and it was reduced by previous treatment with the verapamil. Verapamil was effective in reducing HLEC death mainly through reducing the expression level of apoptosis-related proteins, caspase-3, and increasing glutathione content. Therefore, it was suggested that verapamil was effective in reducing HLEC apoptosis induced by H2O2.

Effects on Range of motion of Neck joint through Five-Animals Micro-Exercises

ZHUO YAN,XU Jiang,HE LI,ZHU WANLING 한국웰니스학회 2018 한국웰니스학회지 Vol.13 No.1

This research was aiming to provide new-styles sport exercises for white and blue-collar workers and prevented people from suffering occupational neck joint illnesses, released the pains from joints of neck and improving the ranges of motions of neck joints throughout the observations that was investigated among staffs who were from a research institute. This research carried out the suggested intervention called Five-Animals Micro-Exercises and then observe its effects that indexes which reflected the flexion, extension, lateral flexion, rotation and pain degree of joint neck would change. There were 320 staffs were chosen from all working staffs who employed by the institute as individual subjects. These 320 staffs did the Five-Animal Micro-Exercise and lasted for 20 weeks during the intervention period. In the progress of intervention, all subjects were divided into groups according to their working locations and organized to do the exercise which lasted for 10 to 15 minutes then exercise frequency was required at least 2 times a day and no more less than 5 days a week. Groups leaders would be elected among the groups and needed to record sport situations of group members. All group members could decide to join the grouped exercise or did the sport alone when they are convenient. The results of intervention would be collected when beginning and ending of this experiment, which is in order to determine the indexes flexion, extension, lateroflexion, rotation, pain degree of joint neck and body attitude assessment. Moreover, 177 subjects who showed higher compliance and reached the required standard of exercising were selected from weekly sport situations subjects’ feedback. The neck joint range of motion had significant changes after intervention, include flexion, extension, lateroflexion, and rotation(p<.05).Besides,, the pain degree and points of body attitude assessment of subjects stated dramatically variation (p<.05).The Five-Animal Micro-Exercise contributes on enlarging the degrees of flexion, extension, lateroflexion, rotation, and pain degree of joint neck are is helpful on improving joint neck degree. It can also relief joint pain, relax muscle, upraises endurance of muscles and increases the coordination of body, which are good for preventing cervical spondylopathy, and help people to maintain their body cures.

Mass transfer and kinetics study on the sulfite forced oxidation with manganese ion catalyst

Zhao Bo,Li Yan,Zhuo Yuqun,Tong Huiling,Zhang Xiaowen,Chen Changhe 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.3

limestone scrubbing is the most common flue gas desulfurization process (FGD) for control of sulfurdioxide emissions from the combustion of fossil fuels. Forced oxidation, which controls the overall reaction of the sulfurdioxide absorption, is the key path of the process. Manganese which comes from the coal is one of the catalysts duringthe forced oxidation process. In the present work, the two-film theory was used to analyze the sulfite forced oxidationreaction with an image boundary recognition technique, and the oxidation rate was experimentally studied by contactingpure oxygen with a sodium sulfite solution. There was a critical sulfite concentration 0.328 mol/Lwithout catalyst ortion constant k; furthermore, we obtained the order with respect to the sulfite and Mn2+ concentrations. When the Mn2+catalyst concentration was kept unchanged, the sulfite oxidation reaction rate was controlled by dual film and the reac-tion kinetics was first order with respect to sulfite while concentration was below 0.328 mol/L; the sulfite oxidationreaction rate was controlled by gas film only and the reaction kinetics was zero order with respect to sulfite while concentration over 0.328 mol/L. When concentration was kept unchanged, the sulfite oxidation reaction rate de-pended on gas-liquid mass transfer and the reaction kinetics was diferent in various stages with respect to Mn2+ con-centrations.

Protectin DX increases alveolar fluid clearance in rats with lipopolysaccharideinduced acute lung injury

Xiao-Jun Zhuo,Yu Hao,Fei Cao,Song-Fan Yan,Hui Li,Qian Wang,Bi-Huan Cheng,Bin-Yu Ying,Bin-Yu Ying,Sheng-Wei Jin 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Acute respiratory distress syndrome is a life-threatening critical syndrome resulting largely from the accumulation of and the inability to clear pulmonary edema. Protectin DX, an endogenously produced lipid mediator, is believed to exert anti-inflammatory and pro-resolution effects. Protectin DX (5 μg/kg) was injected i.v. 8 h after LPS (14 mg/kg) administration, and alveolar fluid clearance was measured in live rats (n = 8). In primary rat ATII epithelial cells, protectin DX (3.605 × 10−3 mg/l) was added to the culture medium with LPS for 6 h. Protectin DX improved alveolar fluid clearance (9.65 ± 1.60 vs. 15.85 ± 1.49, p < 0.0001) and decreased pulmonary edema and lung injury in LPSinduced lung injury in rats. Protectin DX markedly regulated alveolar fluid clearance by upregulating sodium channel and Na, K-ATPase protein expression levels in vivo and in vitro. Protectin DX also increased the activity of Na, K-ATPase and upregulated P-Akt via inhibiting Nedd4–2 in vivo. In addition, protectin DX enhanced the subcellular distribution of sodium channels and Na, K-ATPase, which were specifically localized to the apical and basal membranes of primary rat ATII cells. Furthermore, BOC-2, Rp-cAMP, and LY294002 blocked the increased alveolar fluid clearance in response to protectin DX. Protectin DX stimulates alveolar fluid clearance through a mechanism partly dependent on alveolar epithelial sodium channel and Na, K-ATPase activation via the ALX/PI3K/Nedd4–2 signaling pathway.

Risk Effects of GST Gene Polymorphisms in Patients with Acute Myeloid Leukemia: A Prospective Study

Zhou, Lei,Zhu, Yan-Yun,Zhang, Xiao-Dong,Li, Yang,Liu, Zhuo-Gang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Glutathione S-transferase (GST) enzyme levels are associated with risk of many cancers, including hematologic tumours. We here aimed to investigate the relationships between GSTM1, GSTT1 and GSTP1 polymorphisms and the risk of AML. Genotyping of GSTs was based upon duplex polymerase-chain-reactions with the confronting-two-pair primer (PCR-CTPP) method in 163 cases and 204 controls. Individuals carrying null GSTT1 genotype had a 1.64 fold risk of acute leukemia relative to a non-null genotype (P<0.05). A heavy risk was observed in those carrying combination of null genotypes of GSTM1 and GSTT1 and GSTP1 Val allele genotypes when compared with those carrying wild genotypes, with an OR (95% CI) of 3.39 (1.26-9.26) (P<0.05). These findings indicate that genetic variants of GST and especially the GSTT1 gene have a critical function in the development of AML. Our study offers important insights into the molecular etiology of AML.

Fucoidan inhibits LPS-induced acute lung injury in mice through regulating GSK-3β-Nrf2 signaling pathway

De-Zhang Zhu,Yan-Ting Wang,Yan-Li Zhuo,Kong-Juan Zhu,Xiang-Zhen Wang,Ai-Jie Liu 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.6

The purpose of this study was to investigatethe protective eff ects of fucoidan on Lipopolysaccharide(LPS)-induced acute lung injury (ALI) in mice. The micewere divided into the control, LPS, and LPS + fucoidan(20, 40, or 80 mg/kg) groups. LPS was given by intrachealinstillation and fucoidan was given 1 h before LPS treatment. Myeloperoxidase (MPO) activity, malondialdehyde(MDA), superoxide dismutase (SOD), reactive oxygen species(ROS), glutathione (GSH) contents, and infl ammatorycytokine production were detected. The results showed thatLPS-induced TNF-α, IL-1β, and IL-6 production, lung wet/dry (W/D) ratio, ROS, MDA content, and MPO activity weresuppressed by fucoidan. The levels of SOD and GSH wereincreased by fucoidan. Meanwhile, LPS-induced nuclearfactor kappa-B (NF-κB) activation was dose-dependentlyattenuated by fucoidan. Furthermore, fucoidan increasedthe expression of nuclear factor erythroid-2 related factor2 (Nrf2), Glycogen synthase kinase3β (GSK-3β), and hemeoxygenase (HO-1). In vitro, the results demonstrated thatfucoidan or GSK-3β inhibitor signifi cantly inhibited LPSinducedTNF-α production in A549 cells. And the inhibitionof fucoidan on TNF-α production was blocked by Nrf2siRNA. This study showed fucoidan protected mice againstLPS-induced ALI through inhibiting inflammatory andoxidative responses via regulating GSK-3β-Nrf2 signalingpathway.

Plasma metabolites associated with physiological and biochemical indexes indicate the effect of caging stress on mallard ducks (Anas platyrhynchos)

Zheng Chao,Wu Yan,Liang Zhen Hua,Pi Jin Song,Cheng Shi Bin,Wei Wen Zhuo,Liu Jing Bo,Lu Li Zhi,Zhang Hao 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.2

Objective: Cage rearing has critical implications for the laying duck industry because it is convenient for feeding and management. However, caging stress is a type of chronic stress that induces maladaptation. Environmental stress responses have been extensively studied, but no detailed information is available about the comprehensive changes in plasma metabolites at different stages of caging stress in ducks. We designed this experiment to analyze the effects of caging stress on performance parameters and oxidative stress indexes in ducks. Methods: Liquid chromatography tandem mass spectrometry (LC/MS-MS) was used to determine the changes in metabolites in duck plasma at 5 (CR5), 10 (CR10), and 15 (CR15) days after cage rearing and traditional breeding (TB). The associated pathways of differentially altered metabolites were analyzed using Kyoto encyclopedia of genes and genomes (KEGG) database. Results: The results of this study indicate that caging stress decreased performance parameters, and the plasma total superoxide dismutase levels were increased in the CR10 group compared with the other groups. In addition, 1,431 metabolites were detected. Compared with the TB group, 134, 381, and 190 differentially produced metabolites were identified in the CR5, CR10, and CR15 groups, respectively. The results of principal component analysis (PCA) show that the selected components sufficiently distinguish the TB group and CR10 group. KEGG analysis results revealed that the differentially altered metabolites in duck plasma from the CR5 and TB groups were mainly associated with ovarian steroidogenesis, biosynthesis of unsaturated fatty acids, and phenylalanine metabolism. Conclusion: In this study, the production performance, blood indexes, number of metabolites and PCA were compared to determine effect of the caging stress stage on ducks. We inferred from the experimental results that caging-stressed ducks were in the sensitive phase in the first 5 days after caging, caging for approximately 10 days was an important transition phase, and then the duck continually adapted. Objective: Cage rearing has critical implications for the laying duck industry because it is convenient for feeding and management. However, caging stress is a type of chronic stress that induces maladaptation. Environmental stress responses have been extensively studied, but no detailed information is available about the comprehensive changes in plasma metabolites at different stages of caging stress in ducks. We designed this experiment to analyze the effects of caging stress on performance parameters and oxidative stress indexes in ducks.Methods: Liquid chromatography tandem mass spectrometry (LC/MS-MS) was used to determine the changes in metabolites in duck plasma at 5 (CR5), 10 (CR10), and 15 (CR15) days after cage rearing and traditional breeding (TB). The associated pathways of differentially altered metabolites were analyzed using Kyoto encyclopedia of genes and genomes (KEGG) database.Results: The results of this study indicate that caging stress decreased performance parameters, and the plasma total superoxide dismutase levels were increased in the CR10 group compared with the other groups. In addition, 1,431 metabolites were detected. Compared with the TB group, 134, 381, and 190 differentially produced metabolites were identified in the CR5, CR10, and CR15 groups, respectively. The results of principal component analysis (PCA) show that the selected components sufficiently distinguish the TB group and CR10 group. KEGG analysis results revealed that the differentially altered metabolites in duck plasma from the CR5 and TB groups were mainly associated with ovarian steroidogenesis, biosynthesis of unsaturated fatty acids, and phenylalanine metabolism.Conclusion: In this study, the production performance, blood indexes, number of metabolites and PCA were compared to determine effect of the caging stress stage on ducks. We inferred from the experimental results that caging-stressed ducks were in the sensitive phase in the first 5 days after caging, caging for approximately 10 days was an important transition phase, and then the duck continually adapted.

Estimation of Lipoprotein-lipase Activity (LPL) and Other Biochemical Changes in Two Breeds of Overfeeding Geese

Xu, Hengyong,Wang, Yan,Han, Chunchun,Jiang, Li,Zhuo, Weihua,Ye, Jianqiang,Wang, Jiwen Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.9

This study aimed to examine the effect of overfeeding on biochemical parameters and lipoprotein-lipase (LPL) mRNA expression in different tissues associated with hepatic lipogenesis in Sichuan white and Landes geese. Fifty healthy male Sichuan white geese and fifty male Landes geese (Cygnus atratus) were hatched on the same day under the same feeding conditions and were selected as experimental animals. After overfeeding for 14 days (from 14 weeks to 16 weeks) and then slaughtering, the biochemical changes of hepatic lipogenesis were evaluated. Results showed that i) in Landes geese, the plasma concentration of glucose was higher (p<0.001), while plasma concentrations of insulin and VLDL were both lower (p<0.01); ii) the LPL mRNA level in pectoralis muscle and leg muscle of the overfed groups in both breeds was higher (p<0.05) than in the control groups; iii) in Sichuan white geese, the proportion of fatty liver weight was positively correlated with plasma triacylglycerols (TG)(p<0.05) and VLDL concentrations (p<0.05), while these correlations were not significant in Landes geese; and iv) the activity of LPL had significant positive correlation with the proportions of lipids in subcutaneous adipose tissue and abdominal adipose tissue in Sichuan white geese, while in Landes geese the correlation was negative (p<0.05) with proportions of lipids in the liver, LPL activity had a significant positive correlation with the proportions of lipids in subcutaneous adipose tissue. These results suggest that the Landes geese have a better ability to use the massive amount of ingested food and to store lipids preferentially in the liver, but the Sichuan white geese have a relatively lower ability to use energetic nutrients and lipid storage is more efficient in the adipose tissues.

An Optimized Motion Estimation Algorithm and Application in the FRUC System

Min-Jun Deng,Ping Gan,Zhuo Chen,Xiao-Qing Shen,Dong-Lian Li,Ming-Yan Yu,Yu Zhang,Cai -Lan Zeng,He Huang 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.8

Based on the optimized three-step search algorithm. Combining threshold judgment as well as local full search, a more efficient motion estimation algorithm is proposed. The algorithm not only inherited the traditional three-step algorithm’s quick speed but also kept the advantages of a relatively small amount of calculation, besides it can avoid the local optimum problem in the three-step search algorithm (TSS). In addition, the algorithm combined with the threshold judgment and local full search algorithm, so it also maintains satisfactory visual quality. Comparing the algorithm with TSS and local full search algorithm (LFS). The algorithm has great performance in search points and peak signal-to-noise ratio. Experimental results show that compared with LFS, search points drop by 34.61% ~ 54.47% .While compared with the TSS, the search points only rise by 6.15% ~ 12.21%. The average PSNR of proposed Algorithm is 0.24dB higher than LFS and 3.30dB higher than TSS.