젤여과법을 이용한 토끼 심근 미오신의 분리 및 부분정제

정성탁,김대중,김호덕,라봉진 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.2

Myosin, the major protein required for the production of force and shortening in muscle, is a hexamer consisting two heavy chains (molecular mass∼200kDa) and pairs of light chains (molecular mass∼16 and 20kDa). Myosin and other contractile apparates from the left ventricular myocardium of rabbit were extracted using pyrophospate buffer system and was purified by using the gel filtration. And partially purified each protein component was confirmed by the electrophoresis in non-dissociation and dissociating conditions respectively. On Disc-PAGE of the crude extract, 4 bands clearly appeared and 3 protein components were separated by chromatography in the 5 fractions. In dissociating electrophoresis, each bands were separated into subunit in the pyrophosphate buffer. The first fraction contained the heavy chain of myosin, tropomyosin and myosin light chain 1 and 2. The second and third fraction were composed of tropomyosin and actin and the proteins that had molecular mass smaller than 15kDa were in the fourth and fifith fractions.

유도전동기의 자속제어형 Inverter에 의한 가변속 운전

이승환,우정인,이준탁 동아대학교 공과대학 부설 한국자원개발연구소 1987 硏究報告 Vol.11 No.2

Many techniques for variable speed drives of induction motor had been introduced. Recently, all of them are replaced by PWM control techniques with transitor inverter. But the troublesome problems appeared in variable speed drives include the determination of PWM switching patterns, the generation of control signals, the minimization of torque pulsation, acoustical noises, mechnical vibrations, harmonics and etc.. Therefore, the pursuits of high performance drives and compactness in control circuits without the aids of micro-computer or micro-processor with the functions of control and simple calculations are impossible. In this paper, one technique for the digital and software processing of all control factors is introduced. The determinations of PWM switching patterns are based on the selection of flux vector equivalent to the integral value of applied voltage during every sampling period. And, the real flux vector is controlled so that the trajectory of it can be tracked to the circularform one of ideal flux vector. Also, introduction of the concept of g-u-w plane from d-p plane make digitization and quantization of all control factors convenient. Specially, the predicted control techniques in selection of differential flux vector, Δλ, have functions which can compensate the delay time due to the micro-processor processing, so the real time control system by 8086 micro-processor, simulation and experimental results are in detail compared each other.

行列多項式 LYAPUNOV 函數 構成에 依한 誘導電動機의 安定度 解析

禹靖仁,李浚柝,文在星 동아대학교 공과대학 부설 한국자원개발연구소 1988 硏究報告 Vol.12 No.2

In this paper, when the steady state values for Multidimensional and Nonlinear Dynamic Models of Space Representations are given, Lyapunov functions of scalar types using matrix quadratic polynomials are constructed by Special Matrix Transformation Techniques and applied to Stability Analysis of Induction Motors. Futhermore, Lyapunov function construction procedures for linear systems can be specified in detail. But, unfortunately, for nonlinear systems, there are no the complete construction techniques. Therefore, in this paper, differently from conventional methods using Linearization obtained from Taylor series expansions about steady state operating points, linear terms and nonlinear ones are invidually separated, and by special matrix transformation techniques, similar Lyapunov function construction procedures such as in the cases of linear systems, are introduced, and are applied to Stability Analysis of induction motor. The hyperspaces of multidimensional stability region, obtained from these techniques, were larger than the obtained ones from conventional linearlization techniques about equilibrium points and were assured to be Lyapunov Asymptotic Stability Region ensuring the stable operation of induction motor.

만성 활동성 간염에 있어서 Ursodeoxycholic acid의 치료효과

김성록,안병철,윤영미,탁원영,곽규식,최용환,정준모 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.4

The purpose of this study is to evaluate the clinical and biochemical effects of SGD-F^(R)(UDCA, Vitamin B_1, Vitamin B_2 complex) in chronic active hepatitis. Observed cases were given orally after each meal(three times a day) for 4 weeks. The results were as follows: 1. Among subjective symptoms, improvement rate showed in easy fatigability 86.7%, anorexia 68.0%, indigestion 80.0%, nausea and vomiting 72.7%, RUQ pain 83.3%. 2. Biochemical parameters such as SGOT, SGPT, showed to be improved significantly after the 4 weeks treatment.(p<0.01) 3. There was no specific side effect during the study period in all cases. In conclusion, SGD-F^(R) capsule may be safe and effective for chronic active hepatitis.

기능성 소화불량증환자에서 Winstal® 투여에 관한 임상경험

이창형,김영탁,금민수,권중구,안병철,윤영미,권영오,김성국,최용환,정준모 慶北大學校 醫科大學 1994 慶北醫大誌 Vol.35 No.3

목적 : 기능성 소화불량증은 소화, 흡수등 장관의 기능적인 이상과 관련되어 있을 것으로 생각되며 복합소화효소제(Winstal®)를 투여하여 그 임상효과를 평가하고자 본 연구를 실시하였다. 대상 및 방법 : 1994년 3월 부터 6월까지 경북대학교병원 내과를 방문한 기능성 소화불량증을 호소하는 환자 20명을 대상으로 Winstal®을 1회 1정씩, 1일 3회 식후 30분내에 경구로 2주간 투여하였다. 결과 : 소화불량증의 증상은 복부불쾌감, 복부팽만감, 식욕부진 및 오심, 복부동통, 공기연하증, 고창 및 구토순이었으며, 증상의 개선은 복부불쾌감이 76.4%(13/17)로 가장 높았으며, 그 다음으로 복부팽만감 및 공기연하증이 66.5%(10/15, 6/9)이었고, 오심 61.5%(8/13), 식욕부진 53.8%(7/13), 복부동통 41.6%(5/12), 고창 37.5%(3/8)이었다. 각 환자별 종합적인 증상의 개선도는 현저한 개선이 2예(10%), 중등도 개선이 3예(15%), 약간개선이 11예(55%), 불변이 4예(20%)이었으며 종합적인 유효율은 80%(16/20)이었다. 부작용은 한 예에서도 관찰되지 않았다. 결론 : 본 제제는 기능성 소화불량증 환자의 증상개선에 추천할 만한 유효한 약제로 생각된다. Dyspepsia is a common symptom in gastroenterologic practice and trigger for numerous consultations with physician. The treatment of chronic functional dyspesia is unsatisfactory. This study was performed to evaluate the efficacy of the digestive compound (Winstal®) on 20 functional dyspepsia patients. On open trial, all patients were given 6 tablets daily for 2 weeks and we evaluated the efficacy of this preparation according to the degree of the improvement. The improvement of symptoms was 76.4%(13/17) in abdominal discomfort, 66.5%(10/15, 6/9) in abdominal distension and aerophagia, 61.5%(8/13) in nausea, 53.8%(7/13) in abdominal pain, and 37.5% (5/12) in flatulence, respectively. Overall effectiveness of subjective symptoms was 80% (16/20) and there were no untoward effects of the preparations during this study. As a result, We think that this preparation is an effective one to relieve symptoms of functional dyspepsia.

위장질환에 따른 H.pylori 감염빈도와 검사방법의 비교분석

김대현,조재현,금민수,최성곤,이창형,탁원형,권영오,김성국,최용환,정준모 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : H. pylori가 위장관 질환의 병인에 깊은 관련이 있어 이의 적절한 진단 및 치료가 중요하게 대두되고 있다. 이에 저자들은 소화기 증상을 주소로 내원한 환자들을 대상으로 내시경 검사를 실시하여 H. pylori검출을 위한 CLO검사, IgG 항체검사 및 H&E 염색 등을 시행하여 각 질환별로 H. pylori 감염의 양성율을 알아보고 검사법에 따른 민감도 및 특이도를 그 유용성에 대하여 조사하였다. 대상 및 방법 : 1996 4월에서 8월까지 경북대학교병원에 소화기증상을 주소로 내원한 총 313명의 환자를 대상으로 내시경 검사를 실시하여 H. pylori 검출을 위한 CLO 검사, IgG 항체검사, 조직염색 검사를 실시하였다. 결과 : 전체 대상 환자수는 313명이었고 연령별 분포는 16세부터 80세 까지 다양하였으며 그중40대, 50대, 60대가 각각 69예(22%), 73예(23.3%), 65예(20.8%)로 전체 환자중 66%를 차지하였다. 남녀비는 2.07 대 1로 남자가 많았다. 대상환자의 평균나이는 52±15세였고, 전체 대상환자에서 H. pylori 양성군과 음성군의 평균나이는 각각 50±15세, 54±15세 였고, 궤양환자군에서 H. pylori 양성군과 음성군사이의 평균나이는 각각 49±14.8세, 55±13.8세로 나타났다. 각 질환별분포는 위염이 72명(23%), 십이지장 궤양이 85명(27.2%), 위궤양이 80명(25.6%), 위암이 20명(6.4%), 비궤양성 소화불량이 13명(4.2%), 기타가 43명(13.7%)이었으며, H. pylori 감염 양성율은 위염이 71%, 십이지장 궤양이 86.8%, 위궤양이 58.3%, 위암이 75%, 비궤양성 소화불량이 75%였으며 특히 유문륜 궤양에서는 100%의 양성율을 보였다. 소화성 궤양군과 비궤양군에서의 H. pylori 양성율을 비교해 본 결과 궤양군에서는 73.1%, 비궤양군에서는 72%로 통계학적으로 차이는 없었다(P=0.91). 소화성 궤양환자에서 출혈이 동반된 군과 동반되지 않은 군에서의 H. pylori 양성율을 비교해 본 결과 출혈이 동반된 군에서의 양성율은 54.5%였고, 출혈이 동반되지 않은 군에서는 77.5%의 양성율을 보여 출혈이동반되지 않은 군에서 통계학적으로 유의하게 높았다(P=0.014). 나이에 따른 양성율을 알아본 결과 50세 이하에서는 78.5%였고, 50세 이상에서는 67.5%로서 양성율이 낮았으나 통계학적으로 유의성은 없었다(P=0.052). 각 검사법에 따른 민감도 및 특이도는 전체 환자중에서는 CLO검사가 88.9%, 96.1%, IgG항체검사 90.8%, 58.5%, H&E 염색검사 86.9%, 79.2%로서 민감도는 비슷하게 나왔으나 CLO검사는 상대적으로 특이도가 다른 검사법에 비해 높았다. 궤양군에서의 CLO검사의 민감도와 특이도는 92.0%, 95.6%였고, 비궤양군에서의 민감도와 특이도는 84.3%, 96.8%로서 비궤양군에서는 CLO검사의 민감도는 약간 낮았다. 나이에 따른 CLO 검사의 민감도 및 특이도는 50세 이하에서는 91.5%, 93.1% 였고, 50세 이상에서는 86.3%, 97.9%로서 50세 이상에서는 상대적으로 CLO검사의 민감도가 낮았다. 결론 : 본 연구에서는 위장질환에 따른 H. pylori 양성율을 알아보았는데, 다른 보고에서 처럼 높게 나왔으며, 진단 방법으로 사용한 CLO검사, IgG항체 검사, 조직염색검사 등은 모두 비교적 높은 예민도를 나타냈고, 그중 CLO검사가 간편하고 경제적이라는 면에서 임상적으로 유용한 검사로 여겨지나 상대적으로 CLO검사의 민감도가 낮은 비궤양성 질환과 50세 이상의 환자군에서는 CLO검사 외에 다른 검사방법을 병행함으로써 H. pylori의 진단율을 높일 수 있을 것으로 사료된다. Background: Gastric colonization by Helicobacter. pylori is common among patients with peptic ulcer and gastritis. And various diagnostic tests are available in confirming H. pylori infection. The aim of this paper is to estimate the prevalence of H. pylori infection in Gastroduodenal disorders and compare the sensitivity and specificity of the most widely available tests. Methods: A total of 313 patients were tested for H. pylori infection by IgG Ab, CLO test and H&E stains. Results: The prevalence of H. pylori infection was significantly higher in duodenal ulcer(86.8%) than in gastric cancer(75%), gastritis(71%) and gastric ulcer(58.3%)(P=0.009). The sensitivity and specificity of each test was as follows: CLO test(88.9%, 96.1%), IgG Ab test(90.8%, 58.5%), H&E stain(86.9%, 79.2%). Sensitivity of CLO test in peptic group was 92.0%, whereas in non-peptic group, it was 86.3%. Sensitivity of CLO test in age under 50 was 91.5%, that over 50 it was 86.3%. Conculsions: There is a strong association between H. pylori and gastrointestinal disorders. CLO tests have relatively high sensitivity and specificity, especially in peptic group and younger age(below 50 yr)group. But in those patients in which CLO tests are less sensitive (age>50 or non-peptic group), more than one test may be necessary to diagnose the presence of the organism.

포스터 전시 : 간 15 ; 만성 간질환에서 혈중 gelatinase (Matrix metalloproteinase-2와 9)의 측정 의의

권오상 ( Oh Sang Kwon ),정혁상 ( Hyuk Sang Chung ),주기탁 ( Ki Tak Ju ),정문기 ( Moon Gi Chung ),박동균 ( Dong Kyun Park ),김선숙 ( Sun Suk Kim ),김연석 ( Yeon Suk Kim ),구양서 ( Yang Suh Koo ),김유경 ( Yu Kyung Kim ),최덕주 ( Duc 대한소화기학회 2002 대한소화기학회 추계학술대회 Vol.2002 No.-

Clinical Outcomes of Continuous Addition of Androgen Deprivation Therapy During DocetaxelChemotherapy for Patients With Castration-Resistant Prostate Cancer

Dong Hoon Lee,Jung Ho Kim,Won Ik Seo,Jong Kil Nam,Tae Nam Kim,Cheol Kyu Oh,Soo Dong Kim,Sung-Woo Park,Jae Sung Chung,Sang Hyun Park,Wan Lee,Gyung Tak Sung,Moon Kee Chung,Jae Il Chung 대한비뇨기종양학회 2017 대한비뇨기종양학회지 Vol.15 No.2

Purpose: This study compared the oncologic results of docetaxel chemotherapy (DOC) in castration-resistant prostate cancer (CRPC) according to continuous addition of androgen deprivation therapy (ADT) during chemotherapy. Materials and Methods: We retrospectively reviewed the medical records of 106 patients who received DOC in 6 medical institutes. Among them, 72 patients had a complete medical record: 28 patients with ADT (DOC+continuous ADT group) and 44 without ADT (DOC only group). We compared the progression-free survival of these groups after DOC. Results: Docetaxel was administered an average of 28 months after primary ADT as the first treatment. A median number of 6 cycles of DOC was administered in both groups. In the DOC+continuous ADT group, orchiectomy was performed in 18 patients and luteinizing hormone-releasing hormone agonist was injected in 10 patients. During DOC treatment, prostate-specific antigen (PSA) progression-free survival was statistically different (6.0±4.75 months in DOC+continuous ADT group vs. 4.8±3.2 months in DOC only group, p=0.024), whereas radiologic progression-free survival was not statistically different (5.0±3.12 months in DOC+continuous ADT group vs. 5.0±2.79 months in DOC only group, p=0.387). Conclusions: In our cohort, continuous addition of ADT showed a significant benefit in PSA progression-free survival during DOC in CRPC patients. Further prospective studies are needed to confirm these observations.

위암과 직장암이 병발한 이중 원발성 암 1예

정준모,안병철,김성록,탁원영,곽규식,윤영미,최용한 대한소화기내시경학회 1990 Clinical Endoscopy Vol.11 No.1

Double primary cancer is defined as the case of primary malignant tumors, which must be arise in different sites and have a different histologic appearences. The number of reported cases of double primary malignant tumors has increased in recent and the occurrence of multiple primary cancers in the same individuals is one of the interesting subjects for investigation and research in relation to the carcinogenic mechanism and/or genetic factors. We have experienced a case with double primary malignant tumors of different site origins such as stomach adenocarcinoma and rectal adenocarcinoma, which were diagnosed by biopsies with gastro and colono fiberscopy. For its great rarity, we report this case with review of literatures.

韓國生 참꽃창포(Iris ensata Thunb. var. spontanea Nakai)의 自生地 生育環境 및 形態的 特性에 關한 硏究

鄭鳳鐸,朴仁鉉,劉成吾,朴潤点,裵鍾響 한국화훼연구회 1993 화훼연구 Vol.2 No.2

The present works were carried out to develop wild Iris ensata var. spontanea native to Korea into an flowering plant. Growth environment and characteristics of its morphology were studied. The results obtained are as follows. 1.The Iris ensata var. spontanea has been distributed all over the country, and it has grown naturally in wet soil of the sunnyside sandyloam areas ranged from low mountaintop to 1,300m above the sea level. 2.To study the habitat environment, Goksung is selected where the major habitat for Iris ensata var. spontanea. This study showed that the lowest air temperature was 0.2℃ in January and the highest of 26.2℃ in July to Augut. The maximum air temperature in July was 34.5℃. On the other hand, the minium in January was -6.1℃. The maximum percipitation was 337.0mm in July and the minimum was 19.0mm in October. The number of raining days was 10 in July, and October was 3. The hours of daylight was 229.4 in April and 135.0 in February. 3.Plarrt species showing high frequency rate in the habitat area were Iris ensata var. spontanea, Miscanthus anderss var. purpurasces and Imperata cylindrica var. koenigii. 4.The soil in native area was acidic and soil pH was 5.31, and average humus content, P2O5 SiO2, total N, Ca, Mg, and K were 6.970%, 80ppm 102ppm 0.41%, 4.43me/100mg, 1.28me/100mg, 0.648me/100mg respectively. 5.Among the characteristics of plant height, leaf, rhizome, floral stem, inflorenscence, flower, spathe, stigma, anther, filament, overy, capsule and seed were also surveyed. Plant height was ranged from 50.0cm to 130.0cm. The leaf type was ensiform 2-row alternate, and leaf was costa. The leaf length was ranged from 30.0cm to 70.0cm The characteristics of rhizome were thick, short and brownish. The roots are greyish white, and fiber rich. The spathe type was broad lanceolate and number of spathe was 3. The length of spathe was ranged from 5.0cm to 8.2cm and the width of spathe was ranged 0.4-1.1cm. Flower diameter was ranged from 12.0cm to 18.0cm. Flower colour was red-purple or violet. A flowering duration was from and June to July. Inflorescence type was biflorous, 3 flowers terminal or raceme. The length of floral stem ranged from 46.7cm to 104.1cm and the diameter of floral stem ranged 0.4-0.7cm. The length of pedicel was ranged from 3.2cm to 5.1cm and the dismeter of pedicel ranged 0.2-0.5cm. Outer perianth type was oval. The length of outer perianth segment ranged from 6.9cm to 9.4cm and the width of outer perianth segment, 3.4-4.7cm. Inner perianth type was oblong. The length of inner perianth segment ranged 4.1cm to 5.4cm and the width of inner perianth ranged 0.4-0.9cm. The style branch type was linear-lanceolate. The length of style branch ranged from 3.9cm to 4.7cm and width of style branch ranged 0.7-1.3cm. The style branch crest was two and lacerated triangle. Its top was sharply pushed in, and its bottom was wide and bent down. The length of style branch crest ranged from 0.9cm to 1.2cm. Length of stigma ranged from 2.5cm to 3.7cm and length of anther ranged 1.9-2.3cm. The length of filament ranged from 2.6cm to 3.1cm. Stigma were triangular, white and semi-orbricular. Ovary colour was yellowish green. The length of ovary ranged from 1.7cm to 2.3cm and the width of ovary ranged 0.5-1.0cm. The length of capsule ranged from 2.5cm to 3.0cm and the width of capsule ranged 1.3-1.8cm. Seed colour was brown and seed type was variform. The length and width of seed was ranged from 0.4cm to 0.8cm.