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      • 홍국균의 최적배양조건에 관한 연구

        정순택,임종환,박양균,황금택,박현진,안찬영,강성국,문길만 木浦大學校 工業技術硏究所 1994 工業技術硏究誌 Vol.4 No.-

        Cell growth and pigment productivity of Monascus anka KCCM 11832 and Monascus sp. has isolated from the Korean Maeju(soybean koji) were determined for effect of culture medium composition and incubation conditions. Increase of NH₄NO₃, and KH₂PO₄ concentration as mineral source promoted mycelium growth, and the highest cell growth was showed in the medium with 0.05% KH₂PO₄ when C/N ratio is 20:1, but pigment production was significantly inhibited at higher concentration than this level. Cell growth and pigment production in porato dextrose medium were higher than those in czapeck-dox broth and malt extract medium. In terms of temperature, the highest cell growth was showed at 34℃, but pigment productivity was the highest at 26℃.

      • Ontogeny of the locomotive behavior in a subsocial spider

        Kil Won Kim,Soon Kil Ahn,Kyoung Hye Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

        Post-maternal social organisation of Amaurobius ferox (Araneae, Amaurobiidae) provides an opportunity to investigate the mechanisms of social behaviour in spiderlings while controlling for potential effects of maternal presence. Development and characteristics of locomotory behaviours in young individuals of the subsocial Amaurobius ferox were examined. Young collectively show a very rapid and linear locomotion in a short distance in response to an intruder in the natal nest. Such a locomotory behaviour of the spiderlings resembles the successive synchronised stepping towards prey of the permanently social spider, Anelosimus eximius. We observed the development of different types of locomotion relative to the age of young, collective tendency (i.e., proportion of individuals within the clutch engaging in the behaviour), and directional properties of the locomotion. Locomotory behaviours of spiderlings fell into two categories: ‘abrupt locomotion’ (AL), involving fast and linear movement over a short distance; and ‘ordinary locomotion’ (OL), involving slower movement over longer distances and times. The frequency of AL (abrupt locomotion) did not vary much throughout the days post-matriphagy, occurring for a limited period of time. On the other hand, the frequency of OL (ordinary locomotion) varied over the days post-matriphagy and increased during the maternal social period.

      • KCI등재
      • 무종자의 발아, 묘출현 및 유묘생장에 미치는 Priming의 효과

        안종길,조동,손병구,최영환,강점순,최인수 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.3

        priming 처리제 및 농도에 따라 발아속도에는 차이가 있었는데, 고농도에서 발아속도가 지연되었으나, 저농도에서는 조기발아하였다. 적정 priming 처리제 및 농도는 PEG -0.50 MPa,였다. 무종자의 발아촉진에 최적 priming 조건은 20℃에서 24시간 처리였다. priming 종자는 발아적온 뿐만 아니라 불량발아 조건인 저온과 고온에서 발아력이 높았다. 묘출현율은 priming 처리가 무처리나 수침종자보다 8.1∼4.5% 높았으나, PEG 8000 -0.50 MPa + KH₂PO₄50mM 혼용처리간에는 유의성은 인정되지 않았다. 유묘출현 개시소요일수와 40% 유묘출현 소요일수는 종자처리에 관계없이 2일이 소요되어 종자처리간 차이는 없었다. 파종 25일후 묘생육은 PEG 8000 -0.50 MPa priming처리된 종자는 수침종자나 무처리종자보다 잎생육 촉진되었고 건물증이 증가하는 경향이나, 유의성인 인정되지 않았다. The purpose of this study was to evaluate seed priming influence on germination, emergence and seedling growth in radish. We observed the difference in germination speed on the kinds and concentration of priming chemicals. In the high concentration, we notified the germination was delayed, but in the low concentration, we noticed the germination was accelerated. The optimum concentration of priming chemicals was PEG -0.50 MPa. The optimum priming condition in radish was to treat the seeds for 24hrs at 20℃. The primed seeds showed the improved germination rate in the optimum condition as well as high or/and low temperature. We observed 4.5∼8.1% higher emergence of seedlings from primed seeds than those of non-primed and imbibed. There was no significant difference between PEG 8000 -0.50 MPa and PEG 8000 -0.50 MPa + KH₂PO₄50mM on emergence of seedlings. Start of emergence and days of 40% emergence of seedlings took for 2days regardless of seed treatment. Leaf growth and dry weight 25 days after sowing, were better from primed seeds with PEG 8000 -0.50 MPa than those of non-primed and imbibed.

      • 종자코팅의 고형물질 및 수용성 폴리머가 무 종자의 발아력에 미치는 영향

        안종길,최영환,조동,손병구,강점순 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.2

        코팅종자는 채소, 관상식물을 비롯하여 많은 작물에서 기계화 정밀파종을 위해 사용되고 있다. 코팅의 목적은 종자크기를 증가시켜 기계화 파종을 가능케 하여 파종과 솎음 노력을 절감하고 종자를 절약하는데 있다. 코팅물질의 종류에 따라 발아율이 달랐는데 전반적으로 diatomaceous earth 및 talc +calcium carbonate가 다른 코팅물질에 비해 발아율도 높고 발아속도도 단축되어 무 종자의 종자코팅에 적합한 고형물질이였다. 반면 Limestone, calcium oxide, bentonite 등은 발아율이 저조하였다. 코팅물질로 코팅된 종자는 무처리 종자에 코팅물질을 첨가한 처리에 비해 발아율이 감소하는 경향이었다. 접착제 종류 및 농도에 따라 발아율과 발아속도에는 큰 차이가 있었다. 접착제중 polyvinyl alcohol(PVA) 에서 전반적으로 발아가 원활하였고 다음이 polyvinyl pyrrolidone(PVP), hydroxyethyl cellulose(HEC), methy cellulose(MC), tween 80 순으로 나타났다. 이러한 무처리 종자에 접착제를 공급한 처리보다는 접착제로서 코팅한 종자에서 현저하였다. 코팅배율에 따라 발아율과 발아속도에는 차이가 있었는데, 코팅배율이 낮을수록 신속하게 발아하였다. 무종자에서 적정 코팅배율은 6.0이 좋았다. The use of coated seeds in the production of various field, vegetable, and ornamental crops is a common practice to facilitate precision planting. The purpose of seed coating is to make seeding mechanization possible by enlarging the seed size. After that, it can reduce seeding and thinning labours and can also save seeds. The germination rate was also different according to the coating particulate matters. Generally, percent germination and germination speed of seeds using the mixture of diatomaceous earth, talc and calcium carbonate were higher and faster than using other coating materials, respectively. Otherwise, percent germination using limestone, calcium oxide and bentonite were low, and percent germination of coated seeds with coating material showed the tendency of decreasing more than that of the seeds just added with coating particulate matters. There were significant differences of percent germination and germination speed in accordance with the coating polymer and the concentration. Among the coating polymer, germination of seeds using polyvinyl alcohol (PVA) was generally smooth, and followed by polyvinyl (PVP), hydroxyethyl cellulose (HEC), methyl cellulose (MC), and tween 80. This phenomenon was remarkable in the seeds coated with the polymer than the seeds just added with coating polymer. Also, there were significant differences of the coating size in percent germination and germination speed. Seeds were rapidly germinated as low weight increase of coating seed. The optimal weight increase of coating seed for radish seeds was 6.0 in this study.

      • 고추와 토마토 종자의 발아력 증진에 미치는 Osmotic Priming 및 Solid Matrix Priming의 효과

        안종길,강점순,조동 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.3

        Osmotic priming과 SMP 처리는 종자 발아력을 증진시키는 종자처리이다. Osmotic priming과 SMP 처리과정중 작물별 수분흡수율은 처리한 3시간 이내에 대부분의 수분흡수가 이루어졌다. 최종처리일의 두 처리간 종자함수율은 고추종자에서는 큰 차이는 없었으나, 토마토에서는 osmotic priming이 SMP보다 2.4% 높았다. Osmotic priming과 SMP 처리가 고추와 토마토에서 발아율을 향상시키지는 못했다. 그러나 osmotic priming과 SMP 처리는 평균발아소요일수(MDG) 및 50% 발아에 소요되는 일수(T50)은 단축되어 조기발아를 유도하였다. 고추종자에서는 osmotic priming과 SMP 처리는 무처리 종자에 비하여 T50 이 15℃에서 약 6일, 20℃에서는 약 5일, 25℃에서는 약 4.6일, 30℃에서는 약 4일 정도 단축되었고, 토마토 종자에서는 15℃에서 3.3일, 20℃에서는 1.7일, 25℃에서는 약 4.6일, 30℃에서는 0.5일 정도 단축되었다. 이러한 발아촉진 효과는 불량발아 조건인 저온에서 더욱 뚜렷하였고, 발아적온에 근접할수록 미약해지는 경향이었다. Osmotic priming과 SMP 상호처리간 발아촉진 효과는 고추에서는 SMP 처리가 좋았고, 토마토에서는 큰 차이가 없었다. 종자처리 후 건조방법은 고추는 완전건조가 토마토에서는 표면건조가 조기발아 하는 경향이었다. Osmotic and solid matrix priming treatments enhance germination performance. We compared osmotic with solid matrix priming to determine which was the more effective treatment for improving seed germination in pepper and tomato. Pepper seeds were osmotic primed with 200 mM K₃PO₄and solid matrix were primed with a ratio of 4.0g seed: 1.2 g Micro Cel E: 7.8 ml water for 7 days at 20℃. Tomato seeds were osmotic primed with 150 mM KNO₃and solid matrix primed with a ratio of 4.0 g seed: 1.2 g Micro Cel E: 7.0 ml water for 4 days at 20℃. Seed hydration was immediately observed after osmotic priming and solid matrix priming treatment. The moisture content of solid matrix primed seeds was lower than that of osmotic primed seeds in two vegetable crops. Osmotic priming and solid matrix priming did not increased percent germination, but showed shorter number of days to 50% of the final germination percentage(T50 ) than those untreated seeds regardless of germination temperature. T50 value was reduced in osmotic or solid matrix primed pepper seeds about 6.0, 5.0, 4.6 and 4.0 days than those untreated seeds at 15, 20, 25, and 30℃, respectively. While, that in tomato seeds was reduced about 3.3, 5, 4.6 and 4.0 days than those untreated seeds at 15, 20, 25, and 30℃, respectively. The effectiveness of osmotic priming or solid matrix priming in reducing the T50 was greater when the seeds were germinated at 15℃ than at temperature of higher than 20℃. Solid matrix primed seeds germinated faster than osmotic primed seeds at all temperature in pepper. However, there was no significant difference in seed germination rate between solid matrix and osmotic primed seeds in tomato. After priming, dried-back seeds showed faster germination than surface-dried seeds in pepper. On the other hand, ther was no significant difference in tomato.

      • 광질이 토마토 plug tray 묘의 생육에 미치는 영향

        안종길,강점순,손병구,최영환,이용재 密陽大學校 生命資源開發硏究所 2003 生命資源開發硏究 Vol.7 No.-

        (시험1)착색 셀로판필름과 (시험2)적색광(R)/원적색 광(FR)비 필름을 이용한 토마토묘의 광질반응을 구명 하기 위하여 토마토(挑太郞 8)를 공시재료로 하여 3월 23일에 파종하고 4월 4일~4월 30일간에 (시험1)적색, 청색, 녹색, 황색, 무색투명의 5종류의 착색 셀로판필름과 (시험2)적색광/원적색광 필름을 플러그 트레이 (128공) 육묘상에 터널식 피복처리하여 수행한 결과 (시험1) 셀로판필름의 광투과율이 무색투명, 황색의 순으로 가장 높았고 다음은 적색, 청색, 녹색의 순이었다. 토마토의 초장 및 엽면적등의 생육은 적색이 약간 작았고 녹색, 청색은 초세가 약하여 도장성을 나타내었으나 황색, 무색투명구에서는 묘가 크고 튼튼하였다. 생체중 및 건물중은 지상부, 지하부 모두 황색, 무색 투명군는 컸으나 적색, 청색, 녹색은 작았고 엽록소함량도 적었으며 초세도 연약하였다. 개화기는 황색, 무색투명, 적색의 순으로 빨랐으나 청색, 녹색은 매우 지연되었다. (시험2)R/FR필름의 광투과율이 관행보다 낮았고 토마토묘의 초장이 약간 억제되었으며 엽면적, 생체중 및 건물중도 낮았다. This study was conducted to clarify effect of five cellophane film such as red, blue, green, yellow and transparent color and R/FR film on growth in tomato seedlings(lycopersicum esculentum Mill. cv. Momotaro 8). The tomato was sown in plug tray with 128 hole on March 23, and (Exp.1) five cellophane film and (Exp.2) R/FR film were covered over tomato seedlings from April 4 to April 30, respectively. (Exp.1) The rate of light transmission of each film was the highest in transparent cellophane, and followed in order of yellow, red, blue and green cellophane. The plant height and leaf area were small in red cellophane, green and blue cellophane was weak or rapidly growth, however the plant height in yellow and transparent cellophane was vigor. The fresh and dry weight of seedlings were higher in yellow and transparent cellophane than in red, blue and green cellophane, and also the chlorophyll content was same tendency. The flowering date was earlier in order of yellow, transparent and red cellophane, but was very retarded in blue and green cellophane. (Exp.2) The rate of light transmission was lower in R/FR film than control. The plant height, leaf area, fresh weight and dry weight were small in R/FR film.

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