상추 종자의 코팅 물질 개발과 종자코팅에 영양물질 첨가가 발아력에 미치는 영향

강점순,조동,손병구,최영환,안종길 密陽産業大學校 農業技術開發硏究所 1998 農業技術開發硏究所報 Vol.2 No.1

상추종자는 크기가 불균일하여 기계화 파종이 어렵다. 코팅의 목적은 종자크기를 증가시켜 기계화 파종을 가능케하여 파종과 솎음 노력을 절감하고 종자를 절약하는데 있다. 접착제 종류 및 농도에 따라 발아율과 발아속도에는 큰 차이가 있었다. 사용된 접착제중 polyvinyl alcohol(PVA)에서 전반적으로 발아가 원활하였고 다음이 polyvinyl pyrrolidone(PVP), hydroxyethyl cellulose(HEC), methy cellulose(MC), tween 80 순으로 나타났다. 코팅물질의 종류에 따라 발아율이 달랐느데 전반적으로 diatomaceous earth 및 talc + calcium carbonate가 다른 코팅물질에 비해 발아율도 높고 발아속도도 단축되어 상추종자의 종자 코팅에 적합한 고형물질이였다. 반면 Limestone, calcium oxide, bentonite 등은 발아율이 저조 하였다. 상추종자는 코팅후 종자의 크기가 33배 증가 되었다. 코팅과정중 영양물질 첨가는 발아율이 약간 감소되었고 발아속도도 지연되는 경향이었다. 첨가되는 영양물질의 급원에 따라서도 코팅종자의 발아력에도 차이가 있었는데, 대체적으로 MS medium이 monosodium phosphatic 보다 발아억제 정도가 낮았다. Priming 후 코팅된 종자는 무처리 종자를 코팅한 경우에 비해 발아율이 높았고 조기발아 하는 경향이었다. Seedling mechanization of lettuce is known to be difficult due to the small seed size and the irregular seed shape. The purpose of seed coating is to make seedling mechanization possible by enlarging the seed size. After that, it can reduce seedling and thinning labours and can also save seeds. According to the results, there were signiflcant differences on the percent germination and day to 50% final germination in accordance with the coating polymer. Among the coating polymer, germination of seeds using polyvinyl alcohol(PVA) was generally smooth, and followed by polyvinyl pyrrolidone (PVP), hydroxyethyl cellulose (HEC), methyl cellulose (MC), and tween 80. The germination rate was also different according to the coating particulate matters. Generally, percent germination and speed of seed using the mixture of diatomaceous earth, talc and calcium carbonate were higher and faster than using other coating materials, respectively. Thus, it should be suitable coating particulate matter for the coating of lettuce seeds. On the other hands, germination of seeds using limestone, calcium oxide and bentonite were low. The seed size of lettuce after coating was 33 times as large as the raw seed size. During the process of seed coating, nutrient addition induced the decrement on the germination and the delay of germination speed. Also. there was differences in the germinability of coated seeds in accordance with the addition of nutrient sources. MS medium was generally lower than monosodium phosphate in inhibition of seed germination. Germination of coated seeds after priming was higher than the seeds without the treatment, and also showed the trend of early germination.

Alanine, glycine, proline의 급여가 broiler의 성장 및 도체성적에 미치는 영향

조병욱,신택순,김선구,강한석,이길왕,전해열 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.2

비필수 아미노산 alamine, glycine, proline 등을 첨가한 사료의 급여가 broiler의 성장률 및 도체성적에 미치는 영향을 구명함으로서 아미노산 급여기술의 실용화에 기초 자료를 제시코져 아미노산의 급여수준에 따라 대조구는 아미노산 0%, A구는 alanine 0.5%를, B구는 glycine 0.5%를, C구는 proline 0.5% 를, AB구는 alanine 0.25% 와 glycine 0.25%를, AC구는 alanine 0.25% 와 proline 0.25%를, BC구는 glycine 0.25%와 proline 0.25%를, ABC구는 alanine 0.17%, glycine 0.17%, proline 0.17% 등 8개의 실험구를 배치하여 수행한 실험의 결과는 다음과 같다. 성장률과 사료섭취량 및 사료효율은 아미노산을 급여한 1주일과 2주령까지는 대조구가 높았으나, 3주령과 4주령에는 유의적 차이가 없었고, 몇 개의 아미노산 처리구에서는 성장저하 현상이 나타났다. 대조구와 처리구간의 생체중, 깃털중량, 머리중량, 복강지방의 차이는 나타나지 않았고, 발과 내장의 무게는 처리구들이 대조구보다 낮았다. 도체중, 흉심중량 및 대퇴중량은 유의적 차이가 없었으나, 날개의 중량은 아미노산의 처리구들이 높게 나타났다. 아미노산의 급여가 계속되는 동안에는 명확한 효과가 나타났으나, 중단된 이후 성적의 저하를 나타내는 원인의 규명과 적정한 아미노산의 첨가량, 종류, 첨가시간 및 첨가시기 등 규명해야 할 부분이 많아 앞으로 비필수 아미노산에 이용에 대한 지속적인 연구를 기대한다. This study was conducted to investigate the physio-chemical properties of chicken which was fed with single or mixed amino acids - alanine, glycine and proline. Raw chicken samples were prepared with one of eight treatments : Control : no amino acids ; A : 0.5% alanine added on basal diet ; B : 0.5% glycine added on basal diet ; C : 0.5% proline added on basal diet ; AB: 0.25% alanine and 0.25% glycine added on basal diet ; AC : 0.25%alanine and 0.25% proline added on basal diet ; BC : 0.25% glycine and 0.25% proline added on basal diet ; ABC : 0.17% alanine, 0.17% glycine and 0.17% proline added on basal diet. The physio-chemical properties were analyzed during the storage periods of 1, 4 and 8 days at 4±1℃. Growth performance and efficiency of feeding diet were respectively increased more than the control in the 1-week-old and 2week-old chicks, but there were no significant differences between the treatments and control in 3-week-old and 4-week-old chicks, even though there were growth reduction among the chicks with the supply of some amino acids. There were no significant differences in live, feather, head, and abdomen fat weight between the treatments and control, but the weight of legs and intestinal organs in the treatments were higher than those of the control. There were no significant differences in carcass, breast and leg weight, but the feather weight was much higher in the amino acid treatments than in control. According to the observation, significant effects of amino acids were detected with the maintenance of amino acid supply, but the effects were dramatically dropped with termination of amino acid supply. Therefore, the effects of amino acids such as the amount of supply and the duration of supply are needed to find out for the future studies.

Bacterial Cellulose 생산세균의 분리 및 동정

김용균,김한수,김근기,손홍주 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.2

세균유래 cellulose는 식물유래 cellulose와는 달리 lignin이나 hemicellulose가 전혀 없는 순수한 상태로 생산되며 결정화도, 흡수성 등이 높아 스피커 진동판 등의 산업적 응용 가능성이 높으며, 지혈대, 피부치환제, 식이섬유 등의 용도로서도 개발가능성이 높다. 따라서 자연계에 다양하게 존재하는 세균중 cellulose 생산성이 우수한 균주를 분리 및 동정함으로서 새로운 생물자원을 확보하고자 하였다. 사과에서 분리된 A9균주를 공시균으로 선정하여 형태학적, 배양적 및 생리화학적 특성을 검토한 결과 Acetobacter 속으로 동정되어 편의상 Acetobacter sp. A9으로 동정하였다. 본 균주는 ethanol을 acetic acid로 산화한 후, 다시 C0₂와 H₂O 로 산화하는 특성을 가지고 있었다. 본 균주는 HS 액체배지에서 정치배양 했을 때 두꺼운cellulose pellicle 을 합성하였다. Bacterial cellulose is now being used as transducer diaphragm. In addition, the application of bacterial cellulose to wound dressing and as a carrier for mammalian cell culture has been attempted on the basis of its high water permeability and mechanical strength when moist, as a result of the network structure of the cellulose fibrils forming the membrane. Extensive screening for cellulose-producing bacteria was done using differential media. A total of 57 strains were isolated; the isolate A9 strain from apples was selected and examined to determine its taxonomical characteristics. The bacterium identified as the genus Acetobacter with respect to morphological, cultural and biochemical properties. A9 strain acetic acid from ethanol, and then decomposed acetic acid to C0₂and H₂O. When A9 strain was cultivated statically in Hestrin and Schramm liquid medium, thick cellulose pellicle was formed.

신속하고 균일한 묘출현 증진을 위한 종자전처리 기술

조동,손병구,강점순,최영환,안종길 密陽産業大學校 農業技術開發硏究所 1998 農業技術開發硏究所報 Vol.2 No.1

Preplant seed hydration treatments, particularly at low water potential, mobilized seed's resources that are utilized for rapid and synchronous germination, improved emergence rare, and larger stand size and yields. Seed priming is ideally suited to combat or lessen the impact of a number of soil and climatic constraints, including cold and wet conditions, thermoinhibition, drought, and incidence of diseases. The priming treatment is versatile enough to be integrated with other preplant physiological and non-physiological treatment to combat other internal and external constraints. These include chilling to break the primary dormancy, permeation with hormones to prevent the induction of secondary dormancy, and treatment with pesticides and beneficial microbes to combat seed and seedling diseases during stand establishment. The success or failure of priming treatment are influenced by a complex interaction of factors including plant species, osmeticum, water potential of the priming agent, during of priming, temperature, seed vigor, and dehydration and storage conditions following priming. Seed priming treatment under controlled temperature conditions, allows seeds to imbibe water and go through the initial stages of germination without radicle protrusion through seed coat. The use of a matricum for solid matrix priming(SMP) follows the same principals as for solution osmotic priming. The choice in mainly based on choosing a material wherein water uptake by the seed can be more closely regulated. Concentration of the matricum or osmoticum can be altered to fine-tune water relations The other factors that effect the results from seed priming, aeration, temperature, duration, light. dehydration, and storage are dependent on the species being primed. All crop seeds need air to germinate, thus optimizing aeration during priming. Germination rate and uniformity can be improved by priming for a vast array of crops as evidenced. The numbers of different species commercially primed and available to producers is quite small. The reasons for this might include the high charges to the grower of primed seed, lack of knowledge and or experience by growers of the results obtained from priming. Finally, more practical and essential subjects such as seed vigor and priming effectiveness, effects of dehydration after priming, storage conditions, and the ideal combination of matrix-solid, water, and seeds in SMP treatments are in need of further studies.

제주재래마 특이 표지인자 개발

신택순,김선구,강한석,이길왕,조병욱,전해열 密陽産業大學校 農業技術開發硏究所 1998 農業技術開發硏究所報 Vol.2 No.1

본 연구는 제주재래마의 유전적 특성파악과 이를 통한 타품종과의 유전적 특이성을 가지는 DNA 표지인자를 개발하고자 PCR-RAPD기법을 이용하여 제주재래마와 더러브렛, 가고시마의 3품종에서의 다형 band에 대한 특성파악을 실시한 결과이다. 각 품종별로 10두의 혈액을 혼합한 후 분석하였고 200개의 random primer들 중 PCR증폭양상의 재현성을 기준으로 70개의 primer를 선별하였다. 또한 선별된 primer들중 다형성을 9개의 primer를 이용하여 개체별 DNA분석을 실시하였으며 9개의 prkmer에 의해 나타난 15개의 band는 제주재래마 특이 band, 4개는 더러브렛종에 그리고 4개는 가지고시마 재래마에 품종특이 RAPD band가 나타났다. 따라서 RAPD기법에 의해 품종간의 차이를 보이는 표지인자의 개발이 가능할 뿐 아니라 품종간의 특성차이를 보이는 유전인자 개발과 특성파악이 용이한 것으로 평가되었다. This study was carried out using PCR-RAPD technique to estimate genetic characteristics of Cheju Horse (Jorangmal) and to develop the DNA marker for bread identification in horse. Each three horse breeds was represented by pooled DNA from 10 individuals and tested for 200 different random primers for their reproducible PCR pattern. From them 70 primer were selected and 9 primers were used to detect the difference between breeds at the individual level. Fifteen highly polymorphic bands were observed between breeds using 9 primers . Additionally, the 15 most polymorphic bands showed great difference between breeds in their appearing frequencies, implicating their usefulness in the breed discrimination and in the characterization of genetic structure. Therefore, the polymorphism of RAPD could be used in characterizing genomic difference between breeds and some genetic mechanism that give rise to breed disctimination in horse.

包裝箱子의 理論的 壓縮强度 設計와 劣化要因 分析

朴鍾民 密陽産業大學校 農業技術開發硏究所 1998 農業技術開發硏究所報 Vol.2 No.1

裝籍子 材料로는 대부분 골판지가 이용되고 있으며, 包裝시 사용되던 각종 플라스틱 緩衝材들이 환경오염과 관련되어 그 사용이 규제됨으로써 골판지의 이용이 더욱 확대될 추세에 있다. 따라서 골판지籍子와 골판지 자체에 관련된 제반연구 즉, 골판지의 品質管理. 골판지籍子의 品質規格化, 緩衝效果 및 層積耐久性 향상등 다각적인 연구가 매우 시급하다. 본 연구에서는 우리나라의 주요과실류의 包裝籍子에 대하여 이들 籍子의 수분흡습에 따른 壓縮强度 劣化要因을 분석하였고, 이론적인 壓縮 强度設計 프로그램을 개발하였다. 이 연구의 결과를 요약하면 다음과 같다 (1)包裝籍子의 最大壓縮强度는 골판지의 총평량 보다는 원지의 종류에 더많은 영향을 받 았으며, 상대습도의 증가에 따라 급격히 감소하는 경향이었다. 대체로 온도 보다는 상대습도가 壓縮强度에 미치는 영향이 뚜렷하였으며 籍子의 最大壓縮强鹿는 함수율의 증가에 따라 2차 함수적으로 감소하였다. (2)온·습도에 따른 籍子의 最大壓縮처짐율과 臨界진짐율 모두 상대습도에 따라 거의 직선적으로 증가하였으나, 온도에 따라서는 일률적인 경향이 없었다. 또한 함수율에 따라서 2차 함수적으로 증가하였다. (3) 국·내외의 각종 규정과 자료를 이용하여 包裝籍子의 壓縮强度設計 프로그램을 개발 하였으며, 일부 자료의 부족으로 범용적인 프로그램의 작성이 어려웠으므로 앞으로 계속적인 보완의 필요성이 있다. Major factors in reducing the stacking strength corrugated fiberboard boxes in cold storage or transport conditions are high relative humidity, causing elevated moisture absorption by the boxes. The bottom boxes in a stack will deform to the critical deflection causing agricultural products damage there, and eventually additional deflection will cause box collapse and finally topping of the stack. Therefore, the compression strength of the corrugated fiberboard boxes is very important information to the manufacturers and the end users. The study was conducted to analysis the enviromental condition effects on the compressive strength of the packaging boxes and the develop compression strength design program. The results obtained from the study were summarized as follows: (1) The maximum compressive strength of Box A was about 100 ㎏ greater than that of Box B on the same enviromental conditions. The strength of the sample boxes decreased rapidly with the increasing of relative humidity. The effect of relative humidity on the strength was a little higher than that of temperature. (2) The maximum compressive deflection ratio and the critical deflection ratio of the sample boxes were increased linearly with the increasing of relative humidity, but trends for its ratios showed inconsistant response to temperature. (3) The developed computer program could be applied to only the boxes produced according to the Korean Standards. Also this program needs to be revised continuously by the newly added and developed data.

Alanine, glycine, proline의 급여가 계육의 저장성에 미치는 영향

이길왕,이영근,강한석,신택순,김선구,조병욱 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.2

비필수 아미노산 alanine, glycine, proline 등을 첨가한 사료의 급여가 broiler의 저장성에 미치는 영향을 구명함으로서 아미노산 급여기술의 실용화에 기초 자료를 제시코져 아미노산의 급여수준에 따라 대조구는 아미노산 0%, A구는 alanine 0.5%를, B구는 glycine 0.5%를, C구는 proline 0.5% 를, AB구는 alanine 0.25% 와 glycine 0.25%를, AC구는 alanine 0.25%와 proline 0.25%를, BC구는 glycine 0.25%와 proline 0.25%를, ABC구는 alanine 0.17%, glycine 0.17%, proline 0.17% 등 8개의 실험구를 배치하여 수행한 실험의 결과는 다음과 같다. 흉심근과 대퇴근의 휘발성 염기태 질소의 변화는 저장기간이 경과함에 따라 증가하였고, 흉심근의 휘발성 염기태 질소의 량이 대퇴근보다 높았으며, 흉심근과 대퇴근의 BC처리구의 휘발성 염기태 질소가 모두 유의적으로 높게 나타나 이에 대한 연구가 필요하다고 사료된다. 산패도는 저장기간의 경과에 따라서 증가하였고, 흉심근과 대퇴근에서 단독으로 아미노산을 급여한 처리구들이 대조구보다 높은 산패도를 나타내었고, 혼합급여한 처리구들은 대조구와 유사한 산패도를 나타내서 아미노산의 급여가 지방의 산패에는 부정적 영향을 주나 혼합하여 급여하면 이를 어느 정도 경감시킬 수 있으리라 사료된다. 저장성은 육의 상품가치를 얼마나 오래 유지하는가에 대한 문제이고 경제적인 측면에서 대단히 중요하게 고려하는 문제이므로 비필수 아미노산이 저장성에 미치는 영향에 대한 연구가 수행되기를 기대한다. This study was conducted to investigate the storage characteristics of chicken which was fed with single or mixed amino acids - alanine, glycine and proline. Raw chicken samples were prepared with one of eight treatments : Control : no amino acids ; A : 0.5% alanine added on basal diet ; B : 0.5% glycine added on basal diet ; C : 0.5% proline added on basal diet ; AB: 0.25% alanine and 0.25% glycine added on basal diet ; AC : 0.25% alanine and 0.25% proline added on basal diet ; BC : 0.25% glycine and 0.25% proline added on basal diet ; ABC : 0.17% alanine, 0.17% glycine and 0.17% proline added on basal diet. The storage characteristics were analyzed during the storage periods of 1, 4 and 8 days at 4±1℃. Variation of VBN of breasts and legs were respectively increased as the storage periods were passed, and VBN of breasts was higher than that of legs. In BC treatment, all VBN values of breasts and legs were significantly high, and it should be studied further in this aspect. TBARS also increased as the storage periods were passed. TBARS of breasts and legs, which the chickens were only supplied with amino acid, showed higher than that of control, and that of the groups supplied with mixtures was similar with the control. Therefore, only amino acid supply may be caused negative effects, but the mixture can be reduced the problems. Storage characteristics can be influenced on the maintenance of meat quality and are important in economical aspects. Thus, the effect of non-essential amino acids on meat storage characteristics are needed to find out for the future studies.

Alanine, glycine, proline의 급여가 계육의 연도에 미치는 영향

김선구,조병욱,이길왕,강한석,김용균,전해열,신택순 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.2

비필수 아미노산인 alanine, glycine, proline 등을 첨가한 사료의 급여가 broiler 흉심근의 연도에 미치는 영향을 구명함으로서 아미노산 급여기술의 실영화에 기초자료를 제시코져 아미노산의 급여수준에 따라 대조구는 아미노산의 급여수준 0%, A구는 alanine 0.5%를 B구는 glycine 0.5%를 C구는 proline 0.5%를 AB구는 alanine 0.25% 와 glycine 0.25%를, AC구는 alanine 0.25%와 proline 0.25%를, BC구는 glycine 0.25%와 proline 0.25%를, ABC구는 alanine 0.17%, glycine 0.17%, proline 0.17% 등 8개의 실험구를 배치하여 수행한 실험의 결과는 다음과 같다. 경도, 부착성, 응집성, Gum성, 파쇄성 등은 저장기간의 경과에 따라서 감소하였고, 처리구간의 유의적 차이는 저장초기에만 나타나고 저장기간이 경과하면서 나타나지 않았다. 탄력성은 대조구가 저장기간의 경과에 따라서 감소한데 반해서 처리구들은 거의 변화가 없었다. 따라서 비필수 아미노산을 급여하여 생산된 계육의 조직감은 일반적으로 생산된 계육의 조직감과의 이질성을 나타내지 않아서 생산성의 향상된다면 소비자들에게 쉽게 받아들여질 가능성이 있다고 시료되며, 앞으로 이 분야에 대한 많은 연구를 기대한다. This study was conducted to investigate the tenderness of chicken breast which was fed with single or mixed amino acids - alanine, glycine and proline. Raw chicken samples were prepared with one of eight tretments : Control : no amino acids ; A : 0.5% alanine added on basal diet ; B : 0.5% glycine added on basal diet ; C: 0.5% proline added on basal diet ; AB: 0.25% alanine and 0.25% glycine added on basal diet ; AC : 0.25% alanine and 0.25% proline addd on basal diet ; BC : 0.25% glycine and 0.25% proline added on basal diet ; ABC: 0.17% glycine and 0.17% proline added on basal diet. The tenderness of chicken breast was analyzed during the storage periods of 1, 4 and 8 days at 4±1℃. Hardness, adlhesiveness, cohesiveness, gumminess and brittless were respectively decreased with the increment of storage periods, and the significance among the treatments showed only during the early stage of storage. Elasticity in the control was decreased according to the increment of storage days, but there were no significant changes in the treatments. According to the aforementioned results, it assumed that there was no differences in tenderness of meats in tenderness of meats between the normally bred chickens and the chickens fed with amino acids. Thus, the chicken meats with amino acids can be easily adopted for consumers if the productivity is improved, and it may be need to investigate on this aspect for future studies.

Alanine, glycine, proline의 급여가 계육의 육색에 미치는 영향

이길왕,전해열,강한석,신택순,김선구,조병욱 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.2

비필수 아미노산인 alanine, glycine, proline 등을 첨가한 사료의 급여가 broiler 흉심근의 육색에 미치는 영향을 구명함으로서 아미노산 급여기술의 실영화에 기초자료를 제시코져 아미노산의 급여수준에 따라 대조구는 아미노산의 0%, A구는 alanine 0.5%를, B구는 glycine 0.5%를, C구는 proline 0.5%를, AB구는 alanine 0.25% 와 glycine 0.25%를, AC구는 alanine 0.25%와 proline 0.25%를, BC구는 glycine 0.25%와 proline 0.25%를, ABC구는 alanine 0.17%, glycine 0.17%, proline 0.17% 등 8개의 실험구를 배치하여 수행한 실험의 결과는 다음과 같다. L값은 대조구와 BC, ABC구가 저장기간의 경과에 다라서 유의적으로 증가하는 경향이었고, 다른 처리구들은 유의적 변화가 없었다. 이러한 현상은 육색이 탈색되는 듯한 상태가 되지 않는다는 결과로 바람직한 영향으로 사료된다. 적색도는 저장기간의 경과에 따라 거의 변화가 없거나 감소하는 경향이었고, 저장초기에는 처리구간에 유의성이 나타났으나, 저장 8일에는 처리구간에 유의성이 나타나지 않았다. 저장4일에 A, C, AC, BC 구 등이 급격히 적색도가 증가하는 현상을 나타내었으나 그 원인은 알 수 없었다. 황색도는 전 저장기간동안 A구가 다른 처리구에 비해 유의적으로 높게 나타났고, C구가 낮게 나타났다. C구와 BC구를 제외한 대조구와 실험구들은 저장기간의 경과에 다라서 황색도가 증가하는 경향을 나타내었다. 육색은 소비자의 선택에 중대한 영향을 미치는 요인이므로 육색에 미치는 영향을 규명하는 연구가 계속되기를 기대한다. This study was conducted to investigate the color of chicken breast which was fed with single or mixed amino acids - alanine, glycine and proline. Raw chicken samples were prepared with one of eight treatments : Control : no amino acids ; A : 0.5% alanine added on basal diet ; B : 0.5% glysine added on basal diet ; C : 0.5% proline added on basal diet ; AB : 0.25% alanine and 0.25% glycine added on basal diet ; AC: 0.25% alanine and 0.25% proline added on basal diet ; BC : 0.25% glycine and 0.25% proline added on basal diet ; ABC: 0.17% alanine, 0.17% glycine and 0.17% proline added on basal diet. The color of chicken breast was analyzed during the storage periods of 1, 4 and 8 days at 4±1℃. L-value in the control, in BC treatment and in ABC treatment were respectively increased with the increasement of storage period, although there were no significant differences in other treatments. This phenomenon may be positive effects for the meat markets and consumptions because meat color seemed to be not changed a-value showed no changes or reduction, although there were significant differences among the treatments during the early stage of storage. However, the value showed no significant changes after the 8th days of storage among the treatments. On the 4th day of storage, a-values in A, C, AC and BC treatments were dramatically increased, but the reasons of increments were not known. B-value was significantly higher in A treatment and lower in C treatment than other treatments during the whole storage period. In general, b-values in the all treatments, except C and BC treatments tended to be increased according to the increment of storage days. The various factors influencing meat color, which is an important factor for consumer's meat selection, should be carried out for the future studies.

Alanine, glycine, proline의 급여가 계육의 이화학적 성질에 미치는 영향

강한석,김용균,신택순,이영근,전해열 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.2

비필수 아미노산 alanine, glycine, proline 등을 첨가한 사료의 급여가 broiler의 이화학적 성질에 미치는 영향을 구명함으로서 아미노산 급여기술의 실용화에 기초 자료를 제시코져 아미노산의 급여수준에 따라 대조구는 아미노산 0%, A구는 alanine 0.5%를, B구는 glycine 0.5%를, C구는 proline 0.5% 를, AB구는 alanine 0.25% 와 glycine 0.25%를, AC구는 alanine 0.25% 와 proline 0.25%를, BC구는 glycine 0.25%와 proline 0.25%를, ABC구는 alanine 0.17%, glycine 0.17%, proline 0.17% 등 8개의 실험구를 배치하여 수행한 실험의 결과는 다음과 같다. 흉심근의 pH는 AB구가 높았으며, 흉심근과 대퇴근의 pH는 저장기간이 경과하는 동안 모든 실험구가 유의적으로 증가하였고, 아미노산의 급여로 인한 pH의 변화는 보이지 않았다. 그러나 대퇴근의 pH가 대체로 높아 저장8일에 7.00을 넘는 처리구가 많았다. 수분함량은 휴심근과 대퇴근에서 AB와 ABC구가 높게 나타났고, 이는 pH의 수준에 의한 영향으로 사료된다. 수용성 염용성 단백질의 추출성은 대조구와 처리구간에 두렷한 차이가 나타나지 않았고, pH의 영향으로 생각되는 차이는 나타났으며, 대체로 저장기간의 경과에 따라서 감소하는 경향이었다. 비필수 아미노산의 급여로 인한 계육의 이화학적 성질의 변화를 규명하기 위해서는 앞으로 계속된 많은 연구가 필요하며, 이에 대한 영향이 완전히 규명되어야 실용화가 가능하고, 육의 이화학적 성질은 육의 가치와 이용성을 정의하는 작업이므로 계육의 가공기술을 발전시키는 기초자료로 이용할 수 있으리라 사료된다. This study was conducted to investigate the physio-chemical properties of chicken which was fed with single or mixed amino acids - alanine, glycine and proline. Raw chicken samples were prepared with one of eight treatments : Control : no amino acids ; A : 0.5% alanine added on basal diet ; B : 0.5% glycine added on basal diet ; C : 0.5% proline added on basal diet ; AB: 0.25% alanine and 0.25% glycine added on basal diet ; AC : 0.25% alanine and 0.25% proline added on basal diet ; BC : 0.25% glycine and 0.25% proline added on basal diet ; ABC : 0.17% alanine, 0.17% glycine and 0.17% proline added on basal diet. The physio-chemical properties were analyzed during the storage periods of 1, 4 and 8 days at 4±1℃. pH of breast was higher in AB treatment among the treatments, and pH of breast and leg were respectively increased in the all treatments as the storage period was passed. There was no significant changes in pH with amino acid supply. However, pH of leg was generally higher, for instance, the most of treatments showed the pH values more than 7.0 on the 8th day of storage. For the water content, breast and leg in AB treatment and ABC treatment showed respectively higher values, and in may caused by the different pH values. There was no significant difference in the extraction of water and salt soluble protein between the treatments and the control, even thought there was slight difference caused by the different pH values. In general, the extraction or water and salt soluble protein was reduced according to the increment of storage period. The effect of non-essential amino acids supply on the changes of physio-chemical characteristics of chicken meats should be carried our in near future, and it may useful for chicken meat processing technology.