Streptomyces sp. YSA-130이 생산하는 AIkaline Amylase의 특성

김동섭 密陽大學校 生命資源開發硏究所 2003 生命資源開發硏究 Vol.7 No.-

토양에서 분리되고 강한 protease를 생산하는 균으로 발표된 Streptomyces sp. YSA-130로부터 분리정제된 alkaline amylase를 연구하기 위해 정제된 효소의 특성을 검토하였다. 정제된 효소의 최적온도와 pH는 40℃와 9.0이었으며, pH안정성은 pH 6.0-11.0사이였고 열안정성은 40℃까지 안정하였으며, CaCl_(2) 첨가시 열안정성이 약간 증가하였다. 정제된 amylase의 분자량은 약 25,000 이었으며 PCMB와 PMSF에 의해 강력한 저해를 받았다. The properties of the purified alkaline amylase, produced by Streptomyces sp. YSA-130 which was isolated from soil and published as a strong protease producing microorganism in alkaline condition, were tested. Optimal temperature and pH of the purified enzyme were 40℃ and pH 9.0, respectively. The purified enzyme was stable between the pH range of 6.0 and 11.0. The purified alkaline amylase showed heat stability up to 40℃, and addition of CaCl_(2) increased heat stability of the enzyme. The purified enzyme was inhibited by p- chloromercurybenzoate (PCMB) and phenylmethlsulfonylfluoride (PMSF).

저온기에 통기성 부직포의 파종기별 피복시기가 잎상추의 생육 및 수량에 미치는 영향

안종길,최영환,강점순,손병구,이용재 密陽大學校 生命資源開發硏究所 2003 生命資源開發硏究 Vol.7 No.-

저온기에 노지 및 무가온 하우스 재배시에 통기성 간이 피복재료 파종기별 파종시기 및 피복방법에 의한 잎상추의 생육촉진효과를 구명하기 위하여 공시재료는 청치마와 작치마상추를 이용하였고 농PO계 필름 하우스 내에서 작형별로 '파스라이도' 피복재를 이용하여 1차 파종은 9월 7일에 하여 9월 27에 정식하고 10월 13일부터 10월 31일까지 피복하였으며 2차 파종은 9월 29일에 하여 10월 29일에 정식하고 11월 5일부터 I1월 30일까지 피복하여 그 효과를 구명한 결과는 다음과 같다. 피복기간중의 평균기온, 평균지온 및 평균엽온은 직접+터널피복구, 직접피복구, 터널피복구, 대조구의 순위로 높았고 1차 파종시의 피복보다 2차 파종시의 피복이 매우 낮았다. 일평균 상대습도, 일중평균 상대습도는 직접+터널피복구, 직접피복구, 터널피복구, 대조 구의 순위로 높았고 1차보다 2차 파종시의 피복처리에서 더 높았다. 초장, 엽수, 엽면적등의 생육은 청치마상추, 적치마 상추의 2품종 모두 직접+터널피복구, 직접피복구, 터 널피복구, 대조구의 순위로 1차 파종시 보다 2차에서 매우 저조하였다. chlorophyll 함량은 작형별로 2품종 모두 대조구, 터 널피복구, 직접피복군, 직접+터널피복구의 순으로 높았고 1차 파종시 보다 2차에서 약간 낮았다. 생체중은 2품종 모두 직접+터널피복구, 직접피복구, 터널피복구, 대조군 순위였고 2차 파종시의 파복이 1차보다 50%이상의 감량을 나타냈었다. 건물중도 같은 경향을 나타내었으나 처리간의 차이는 적었다. This study was conducted to clarify effect of cultivar, covering method and seeding date on growth in leaf lettuce during low temperature season. 'Chungchima' and 'Jukchima' leaf lettuce were seeded on Sept. 7(1st sowing) and on Sept. 29(2nd sowing), and planted on Sept. 27 and on Oct. 29, respectively Lettuce cultivars sown 1st time were covered with 'pasrido' film in one kind of PO film house, Oct 13 to Oct. 31, and these sown 2nd time covered Nov. 5 to Nov. 30. The mean air temperature and mean soil temperature and mean leaf temperature were high in order of direct+tunnel covering, direct covering, tunnel covering and control, and lower in 2nd sowing time than in 1st sowing time. Daily mean relative humidity and daytime mean relative humidity were high in order of direct+tunnel, direct, tunnel and control, and higher in 2nd sowing time than 1st sowing time. The plant height, leaf number and leaf area of two cultivar were increased in order of direct+tunnel, direct, tunnel and control, and worse in 2nd rowing tune than 1st sowing time. Chlorophyll content of two cultivar was higher in order of control, tunnel, direct and direct+tunnel, and lower in 2nd sowing time than 1st sowing time. The fresh weight of two cultivar was higher in order of direct+tunnel, direct, tunnel and control, and more weight loss of 50% over in 2nd sowing time than in 1st sowing time. Also dry weight showed similar tendency, but the difference among treatment was a little.

옥색긴꼬리 산누에나방의 조직별 수용성 단백질의 전기영동패턴과 수종의 산누에나방의 종간의 체액단백질비교

조현주,이호주,장보영,최계종,박남숙,박상봉,이상몽 密陽大學校 生命資源開發硏究所 2002 生命資源開發硏究 Vol.6 No.-

Haemolymph and soluble proteins in several tissues of several lepidoteran species were investigated electrophoretically by native-PAGE and SDS-PAGE. The eletrophoretic profiles of the soluble proteins in the haemolymph, fat-body, silkgland, midgut and integument of the wild silkmoth, Actias gnoma, were monitered very differently. Also in the comparison of haemolymph proteins among lepidopteran insects, the electrophoretic patterns varied greatly. These results reveals that different tissues or organs present in a same insect species can have their own soluble protein patterns, and even though in a same tissue (haemolymph), speciesdependant electrophoretic patterns can be generated.

전국 목조 문화재의 생물열화에 의한 피해 조사

박현철,이광석,김정열,노태경 密陽大學校 生命資源開發硏究所 2002 生命資源開發硏究 Vol.6 No.-

According to the surveys of biodegradation on wooden construction buildings including National Treasures and Treasures in national wide, most of wooden cultural assets in this country were damaged by various biological agents including termites, although it was shown more damages in the southern part of Korea. Most of damages usually found on the wooden structure near the ground or within lm height, and the damages were hardly found on the structure around ceiling or roof area. According to the correlation between moisture inside of the woods and biodegradation, there were close relationship between moisture and biodegradation. Most of damaged woods contained more than 15% of moisture. On the other hand, moisture in undamaged woods was less than 12%.

양질 녹용의 대량 생산기술 개발

이길왕,김상우 密陽大學校 生命資源開發硏究所 2002 生命資源開發硏究 Vol.6 No.-

Deer (Cervidae) have been important to human since a long time ago. They were valuable for human being as sources of meat, skins for clothing and bones and antlers for tools and weapons. Deer have been an important game and sporting animal in the world, and in the oriental countries, especially in Korea, China and Taipei, they have been a precious being for human health as its velvet for Chinese medicine over the last hundred years. Because deer are now becoming valuable farm animals, they are numerous in many temperate areas. Our country has the largest market and has been most imported for velvet, the main products of deer farming, in the world. There was rapid development of deer farming in our country shared with much consumption of velvet. By the results of our researches, velvet production was the main source of income in deer farming, but the production was not efficient due to unscientification of feeding management. And also the feed was used with those for other animals in most deer farm and yet feed for deer was not development in earnest. It has been caused by immoderate introduction of foreign data for venison production or for goat and no establishment of standard feeding system which is suitable in the actual condition of our deer farming for velvet production. This study was conducted in order to development of the producing technique of young antlers of good quality in deer. Casting of staghorn was achieved on 16day±3day after MPA treatment. Could advance about month that do pleasure the time of horn casting account as that MPA treatment. It was no difference between control group and MPA group for yield of velvet antlers of the deer by each 712g and 718g. Testosterone density in blood looked tendency that increase according as growth of velvet antlers of the deer is proceeded. Testosterone level that is concerned at horn casting for keratinization of velvet antlers of the deer MPA group did not increase greatly. IGF-1 density in blood looked tendency that decrease according as young antlers of the deer growth is proceeded. IGF-1 level of MPA group is higher than control group at staghorn cutting.

집오리의 생체중에 따른 도체 생산량에 관한 연구

김선구,강한석 密陽大學校 生命資源開發硏究所 2003 生命資源開發硏究 Vol.7 No.-

집오리 28수로부터 생체중에 따른 도체 생산량을 조사하였다. 오리의 생체중이 1669.8g에서 2472.5g까지 증가함에 따라 도체율이 61.6%에서 69.4%로 증가 하였다. 생체중(x)과 도체율(y^)의 관계는 y^=46.773+ 0.995x로 추정되었다. 생체중에 따른 부분육의 생산비 율은 차이가 보이지 않았으며, 부분육의 생산량은 가슴 부분 24.8%, 대퇴부와 하퇴부 21.3%, 날개부 14.8%, 등과 허리가 각각 1l.7%와 15.5%를 나타내었다. 생체중에 대한 가식 내장 생산비율은 간 2.26%, 심장 0.76%, 근위 3.44%이었다. Twenty-eight heads of domestic ducks were used to investigate the yields of carcass as influenced by live weight. The dressing percentages were increased 61.6% to 69.4% with increasing live weight from 1669.8g to 2472.5g. The regression equation of dressing percentage(y) on live weight(x) was y=46.773+0.995x. The yields of cut-ups on carcass weight were 24.8% in breast, 21.3% in tights and drums, 14.8% in wings, 11.7% in back and 15.5% in loin. The yields of giblets on live weight were 2.26% in liver, 0.7655 in heart and 3,44% in gizzard.

남은 음식물을 이용한 오리 사육 시험

강한석,전해열,신택순,김선구 密陽大學校 生命資源開發硏究所 2003 生命資源開發硏究 Vol.7 No.-

본 연구는 남은 음식물을 가축에게 이용하기 위하여 개발된 발효사료를 이용하여 오리를 사육했을 때 사양 효과를 검토하기 위하여 수행되었다. 육추가 끝난 16 일령 오리 80마리를 공시하여 배합사료의 50%를 남은 음식물 사료로 대체 급여하고 급여기간에 따라 대조구(0일), T_(1)(10일), T_(2)(15일), T_(3)(20일), T_(4)(25일)의 5개 처리에 배치하였다. 오리들은 57일령에 도살되었다. 시험완료시 체중은 대조구, T_(1), T_(2), T_(3) 및 T_(4)에서 각각 2376.Og, 2313.1g, 2308.1g, 2235.8g 및 2269.6g 으로 처리간에 차이가 없었다. 처리별 도체율은 각각 69.6%, 69.4%, 66.6%, 64.1% 및 70.8%로 T_(2)와 T_(3)에서 유의적으로 낮은 편이었다. (p<0.05) 가식내장 중 근위의 무게는 각각 78.7g, 78.6g, 71.79g, 66.4g, 76.0g으로 T_(2)와 T_(3)에서 낮은 편이 었다. (p<0.05) 본 시험결과 육용오리 사육시 남은 음식물 사료를 25일 간은 농후사료의 50%를 급여해도 좋을 것으로 나타났다. This study was carried out to investigate the feeding effects of food waste in growing duck. The food waste was fermented before being used as a substitude of duck feed. The substitution level of fermented food waste during experment was 50% of commercial broiler feed. Eighty ducklings, 16days old age, were allocated to five treatments. The feeding periods of fermented food waste were control, 0 day ; T_(1), 10 days ; T_(2), 15 days ; T_(3), 20 days and T_(4), 25 days. Five ducks in each treatment were slaughtered at 57 days old. The live weight of control, T_(1), T_(2), T_(3) and T_(4) at 57 days old were 2,336.0g, 2,313.1g, 2,308.1g, 2,235.8g and 2,269.6g, respectively. and there were no significant difference among treatments. Dressing percentage of control, T_(1), T_(2), T_(3) and T_(4) were 69.6%, 69.4%, 66.6%, 64.1% and 70.8%, respectively, and those of T_(2) and T_(3) were significantly lower than other treatments(P<0.05) The yields of gizzards of control, T_(1), T_(2), T_(3) and T_(4) were 78.7g, 78.6g,71.7g, 66.4g and 76.0g, respectively, and those of T_(2) and T_(3) were significantly lower than other treatment(P<0.05) This results indicated that the food waste could be used as a substitude of growing duck feed for 25days.

强制換羽 産卵鷄에서 産卵能力과 卵質의 變化

安性讚,金善丘 密陽大學校 生命資源開發硏究所 2002 生命資源開發硏究 Vol.6 No.-

This study was carried out to investigate the change of egg production and egg quality in forced molted hen layers. A total of 235 Isa-Brown hens, 80weeks of age, were fasted for 7, 10, 12 or 14days. Egg production and egg quality were evaluated for 26 weeks of production following initiation of fasting. Fifty percentage of egg production appeared at the 5∼6th week after beginning of fasting and peak production was shown at the 10th week. The rate of egg production decreased gradually since the 15th week after beginning of fasting. Egg weight increased to 14th week and thereafter decreased gradually. Albumen height and Haugh-unit were high at the 4th week after beginning of fasting and decreased since 22th week after beginning of fasting. Egg shell weight was increased to the 8th week and maintained to the 22th week. Shell and yolk colour darkness were decreased after molting, but increased since the 6th week after beginning of fasting. Shell density and thickness were increased to the 8th week and after then decreased gradually.

光度, CO_2濃度가 들깨의 光合成速度 및 그 關聯形質에 미치는 影響

李忠烈,金成萬,金容澈,崔仁洙,朴賢哲 密陽大學校 生命資源開發硏究所 2002 生命資源開發硏究 Vol.6 No.-

In order to determine the effects of CO_2 concentration on the net photosynthetic rate, transpiration, stomatal conductance and intercellular CO_2 concentration, perilla was planted in a field. The net photosynthetic rate were increased as the PAR was increased to 700∼1000μ mol/m^2/s in perilla leaves, and was showed higher elevated CO_2 concentration(600ppm, 900ppm) than the control(350ppm). The intercellular CO_2 Concentration was showed higher under low illumination than under high illumination, but was decreased with the increment of light intensity. There were positive correlation with the transpiration rate and stomatal conductanec. Different a linear equations were respectively obtained between net photosynthetic rate and stomatal conductance, and between net photosynthetic rate and transpiration rate in barley.

염료자원식물의 분류에 관한 연구

하순혜,손병구 密陽大學校 生命資源開發硏究所 2003 生命資源開發硏究 Vol.7 No.-

본 연구는 국내외 이용되는 염료식물 총 312종류를 과별, 이용부위, 매염제, 색상 등에 대한 조사로 그 결과는 다음과 같다. 1. 염료식물의 종 조성은 91과 213속 293종 19변종으로 총 312종류이다. 그 중 장미과가 가장 많으며, 국화과, 콩과, 단풍나무과, 참나무과 순서이나, 대다수의 과에서 골고루 나타났다. 2. 이용부위 별로는 대부분이 신엽, 엽, 풀, 가지, 수피 등 이며 종종 꽃봉오리, 수술, 뿌리, 충영도 사용되었다. 3. 매염제로는 알루미늄, 철, 구리, 잿물이 가장 많았으며, 식물이 따라 가끔 크롬, 석회 등도 사용되었다. 4. 색상은 자연의 느낌인 녹색계와 갈색계가 대부분이며, 황색, 적색, 청색계통도 나타났다. This research aims to classify a total of 312 domestic and foreign dye plants on the basis of their family, parts used, catalysts and color. The results of this research can be summarized as follows: 1. The total number of 312 dye plants can be classified into 91 families, 213 genera, 293 species, and 19 varieties. Of these, Rosaceae is most common, and then Compositae Leguminosae, Aceraceae, and Fagacceae in that order. Their distribution is generally even. 2. The parts used (in the order of frequency) are fresh leaf, leaf, grass, branch, and bark. Buds, stamens, root and galls are also used sometimes. 3. The most common dying catalysts are aluminum, iron, copper, and ash juice. Chrome and lime are also used for some plants. 4. Their dying color is mostly green and brown, and sometimes yellow, red and blue.