http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Yoo, Si-Youl,Min, Junhong,Haga, Tomoaki,Hirakawa, Hidehiko,Nagamune, Teruyuki,Choi, Jeong-Woo American Scientific Publishers 2017 Journal of Nanoscience and Nanotechnology Vol.17 No.8
<P>Various kinds of biologic gates have been developed to realize biocomputing system with enzyme reactions. However, logic gate with summation function based on putidaredoxin reductase and cytochrome c has not been reported yet. Here, we developed biologic gate with summation function. To fabricate the biologic gate system, putidaredoxin reductase was immobilized on two gold electrodes for input system and cytochrome c was immobilized on one gold electrode for reading output signal. Then polydimethylsiloxane based microfluidic chip was fabricated on the gold electrodes and each electrode was connected with microfluidic channels. To operate the biologic gates, two AND gates with putidaredoxin reductase and one OR gate with cytochrome c was checked by confirming enzyme reactions firstly. And then, we checked summation function with fabricated two AND gates and one OR gate. The benzoquinone injected to each putidaredoxin reductase was acted as input and it was defined to '0', '1' and '2' by different concentrations. Two input signals were flowed to cytochrome c and summated signal was checked by cyclic voltammetry. The summated output signals were defined from '0' to '4' with various thresholds of current intensity. Proposed enzyme logic gate with summation function can be applied to bioprocessing and biocomputing system.</P>
Influence of oxygen on the microstructural growth of SiC nanowires
Choi, Yoo Youl,Kim, Jun Gyu,Park, Si Jung,Choi, Doo Jin Elsevier 2012 Chemical physics letters Vol.531 No.-
<P><B>Graphical abstract</B></P><P><ce:figure id='f0030'></ce:figure></P><P><B>Highlights</B></P><P>► The influence of O<SUB>2</SUB> on the growth of SiC nanowires by CVD was examined. ► O<SUB>2</SUB> content is highly related to the crystallization and density of SiC nanowires. ► Crystallization of SiC nanowires improved as deposition temperature increased in O<SUB>2</SUB> environments.</P> <P><B>Abstract</B></P><P>We examined the influence of oxygen (O<SUB>2</SUB>) on the growth of silicon carbide nanowires (SiC NWs) by chemical vapor deposition (CVD), because vacuum environments are susceptible to O<SUB>2</SUB> leakage inflow. We investigated the effect of increasing the O<SUB>2</SUB> flow rate on the microstructural changes of SiC NWs and then evaluated the effect at various growth temperatures. The O<SUB>2</SUB> content was highly related to the density of NWs, which continued to increase actively at higher temperatures. High-resolution transmission electron microscopy (HR-TEM) revealed that NWs change from a single-crystalline phase to a polycrystalline phase and then to a SiC/SiO<SUB>2</SUB> core–shell structure.</P>
DNA-Recombinant Azurin Conjugation as a Biomemory Platform with Enhanced Sensitivity
Chen, Qi,Yoo, Si-Youl,Lee, Taek,Kim, Sang-Uk,Nam, Eun Sook,Min, Junhong,Choi, Jeong-Woo American Scientific Publishers 2016 Journal of Nanoscience and Nanotechnology Vol.16 No.11
<P>Biocomputing devices with DNA, protein/enzymes and other biomaterials have been studied to develop unconventional computing devices. However, the high sensitivity of these devices is still in doubt due to difficulty detecting the efficient electron transfer between biomolecules and the device surface. In this study, oligonucleotides were introduced to the recombinant azurin to enhance the current signal. The recombinant azurin self-assembled on the gold surface due to the formation of gold-sulfur covalent bonds. Single stranded DNA (ssDNA) sequences were further conjugated with self-assembled recombinant azurin via a chemical linker. The fabricated biofilm was confirmed by cyclic voltammetry (CV) and atomic force microscopy (AFM). Moreover, enhancement of the electrochemical signal sensitivity and durability to harsh pH conditions were achieved by conjugation with biomolecular oligonucleotides that can contain Pb2+. The biofilm consisting of DNA and recombinant azurin was further verified to be a suitable platform for biomemory. The sensitivity and durability of the biomemory platform was enhanced by simply by introducing DNA into the system, without any other labels or nanoparticles. The proposed biofilm can be applied to bioelectronics devices such as bioprocessors and biocomputers.</P>
Controlled releasing properties of gelatin nanofabric device containing chlorhexidine
( Dae-ung Park ),( Heung-sik Um ),( Beom-seok Chang ),( Si-young Lee ),( Ki-yeon Yoo ),( Won-youl Choi ),( Jae-kwan Lee ) 조선대학교 치의학연구원(구 조선대학교 구강생물학연구소) 2021 Oral Biology Research (Oral Biol Res) Vol.45 No.2
We aimed to evaluate the degradation and antimicrobial activity of novel chlorhexidine-containing gelatin nanofabric devices and their cytotoxicity in animals. The electro-spun device with a size of 3.4.0.4 mm<sup>3</sup> was prepared by entrapping chlorhexidine gluconate (CHX) in a gelatin matrix. The devices were divided into three groups based on the CHX percentage (G1: 46%, G2: 50%, and G3: 54%), and the commercial product, PerioChip (CP), was used for the control group. We used an in vitro test for the degradation and antibacterial activity and investigated cytotoxicity using an in vivo test. Artificial saliva was used for the degradation test of chips and blood agar plates seeded with the oral bacteria Streptococcus sanguinis to address antibacterial activity. Furthermore, the devices were inserted between the skin and muscle of rat abdomens to evaluate infection and inflammatory reactions. We detected no obvious differences in the antibacterial or degradation test results between the test and control groups. In the histological analysis, the scaffold without CHX showed no signs of infection or inflammatory reaction. Both CHX-containing groups exhibited inflammation and necrosis in the muscle and skin, although the reaction in the test group was milder. In the degradation tests, antibacterial tests, and cytotoxic reactions in animal experiments, the electro-spun gelatin-based device showed similar results to those of the commercial device in the control group. Further studies on the quantitative analysis and clinical outcome evaluation of the electro-spun gelatin-based device in humans are necessary.
2급 응급구조사 양성과정 개선방안 연구 : 양성기관 현황 및 지정기준 검토
이남종,신동민,김병우,박시은,유은지,윤병길,윤성우,윤형완,이경열,최재웅,황성훈,Lee, Nam-Jong,Shin, Dong-Min,Kim, Byung-Woo,Park, Si-Eun,Yoo, Eun-Ji,Yoon, Byoung-Gil,Yun, Seong-Woo,Yun, Hyeong-Wan,Lee, Kyoung-Youl,Choi, Jae-Woong,Hwang, 한국응급구조학회 2019 한국응급구조학회지 Vol.23 No.2
Purpose: To discuss the records and legal standards of emergency medical technician training institutions in Korea and abroad, to identify the problems, and to provide the basic resources for improving the EMT-basic training institutions. Methods: We received advice through an advisory meeting of experts (professors of department of emergency medical technicians) and interested parties (Korean Association of Emergency Medical Technicians, officials of emergency medical technician training institutions) and referred to various reports published by governments, official institutions, and other trustworthy organizations. Also, we communicated with the related experts abroad (3 countries) on the phone or by email for surveys. Results: Compared to the abroad, it is necessary to categorize the standards and procedures of designating the emergency medical technician training institutions in Korea and improve the management of training institutions to train competent emergency medical technicians. Conclusion: It is necessary to designate and manage continuously the emergency medical technician-basic training programs for the systematic primary healthcare service.