Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events

Yoo, Min-Sang,Shin, Minguk,Kim, Younghun,Jang, Min,Choi, Yoon-E,Park, Si Jae,Choi, Jonghoon,Lee, Jinyoung,Park, Chulhwan Elsevier 2017 CHEMOSPHERE - Vol.175 No.-

<P><B>Abstract</B></P> <P>We developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of <I>Bacillus subtilis,</I> one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-<I>B. subtilis</I> were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (10<SUP>2</SUP>–10<SUP>10</SUP> CFU/mL) and the detection limit (10<SUP>2</SUP> CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target <I>B. subtilis</I> was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (<I>Staphylococcus aureus</I>, <I>Flavobacterium psychrolimnae</I>, and <I>Aquabacterium commune</I>). In addition, target <I>B. subtilis</I> detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A SWCNTs-based biosensor was developed for the detection of <I>Bacillus subtilis.</I> </LI> <LI> The biosensor was composed of SWCNTs, 1-PBSE, and anti-<I>B. subtilis</I> antibody. </LI> <LI> The performance of the biosensor was assessed using a sensor sensitivity and specificity tests. </LI> <LI> The detection limit and detection range were 10<SUP>2</SUP> and 10<SUP>2</SUP>–10<SUP>10</SUP> CFU/mL, respectively. </LI> <LI> The detection time of the biosensor was identified as 10 min. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

An automated quality assurance system with deep learning for small cube-ball phantom localization in noisy megavoltage images

Park Min-Jae,Lee Gyemin,Kwak Jungwon,Jeong Chiyoung,Goh YoungMoon,Kim Sung-woo,Cho Byungchul,Song Si Yeol,Kim Jong Hoon,Jung Jinhong,Shin Young Seob,Oh Jungsu 한국물리학회 2024 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.84 No.9

To enhance effi ciency and minimize errors, we automated the quality assurance (QA) process in radiation oncology, specifi - cally laser localization. Additionally, we explored the use of a convolutional neural network (CNN) to enhance the detection of small cube-ball phantoms in noisy images. Laser localizations were measured manually on the acquired images. To automate the QA workfl ow, we developed a Linux server equipped with database and web servers. Digital Imaging and Communications in Medicine (DICOM) fi les were retrieved 40 times for 10 linear accelerators (LINACs). The center of the cube-ball phantoms was estimated through Gaussian fi tting. We applied CNN using 6,968 stored results to improve the estimation performance in noisy megavoltage (MV) images. Subsequently, both analysis time and accuracy were compared. Our hospital has been employing the automated QA system since 2018, notably reducing the time for laser localization from 30 min to just 1 min. The average and standard deviation (SD) of inter-observer variability in the X- and Y-axes were 0.06 ± 0.01 mm and 0.05 ± 0.01 mm, respectively. Absolute diff erences between manual assessment and Gaussian fi tting presented average and SD values of 0.40 ± 0.51 mm and 0.23 ± 0.24 mm, respectively. In contrast, absolute diff erences between manual assessment and CNN presented average and SD values of 0.12 ± 0.10 mm and 0.11 ± 0.09 mm, respectively. Overall, the automated QA system signifi cantly hastened procedures in our large hospital and improved the estimation of the cube-ball phantom’s position in noisy images through deep learning.

붕장어 부산물로 제조한 붕장어탕의 식품학적 특성

허민수(Min Soo Heu),이택상(Take Sang Lee),김혜숙(Hye-Suk Kim),지성준(Seung Joon Jee),이재형(Jae Hyoung Lee),김형준(Hyung Jun Kim),윤민석(Min Seok Yoon),박신호(Shin Ho Park),김진수(Jin-Soo Kim) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.4

붕장어 가공부산물인 머리와 frame를 효율적으로 이용하기 위한 일련의 연구로 붕장어 부산물을 이용한 붕장어탕의 개발을 시도하였고, 아울러 이의 식품성분 특성에 대하여도 살펴보았다. 살균조건 및 가온일수에 따른 붕장어탕의 저장성 부여를 위한 최적 살균조건은 F? value=8분으로 판단되었다. 붕장어탕의 일반성분은 수분의 경우 90.7%, 조단백질의 경우 4.8%, 조지방의 경우 2.6%, 조회분의 경우 1.5%를 나타내었다. 붕장어탕의 엑스분 질소 함량은 243.1 ㎎/100 g으로 시판 추어탕의 208.0 ㎎/100 g보다 높았다. 붕장어탕의 총 아미노산 함량은 4,310 ㎎/100 g이었고, 주요 구성아미노산은 glutamic acid(637.3 ㎎/100 g, 14.8%), glycine(409.1 ㎎/100 g, 9.5%) 및 alanine(404.4 ㎎/100 g, 9.3%)등이었다. 붕장어탕은 관능적인 비린내는 거의 감지되지 않았고, 맛은 우수하였으며, 항산화능이 인정되었으나 ACE 저해능은 크게 기대할 수 없었다. For the effective use of the conger eel by-products, such as head and frame, Tang, which is the Korean-type soup, from conger eel by-products (TCEB) was developed and its food component characterization was compared with that of commercial Chueotang, loach Tang. According to the results of viable cells and coliform group of TCEB heated at 115oC for various times, the reasonable F0 value was 8 min. The proximate composition of TCEB was 90.7% for the moisture, 4.8% for the protein, 2.6% for the lipid, and 1.5% for the ash. The extractive-nitrogen content of TCEB was 243.1 ㎎/100 g, which was higher than that of commercial Chueotang, 208.0 ㎎/100 g. The total amino acid content of TCEB was 4,310 ㎎/100 g and its major amino acids were glutamic acid (637.3 ㎎/100 g, 14.8%), glycine (409.1 ㎎/100 g, 9.5%) and alanine (404.4 ㎎/100 g, 9.3%). TCEB was not felt in the sensual fish odor and its sensual taste was good. The health functional properties for health of TCEB were 1.29 as a PF (protection factor) for antioxidative activity and 39.4% for angiotensin Ⅰ converting enzyme (ACE) inhibiting activity.

허혈 유사 조건이 심방근의 활동전위 특성에 미치는 영향

김재하,주정민,조용범,신동호,안병희,주신배,김상형 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11

Poly(vinylbenzyl chloride-glycidyl methacrylate)/Polyethylene Composite Anion Exchange Membranes for Vanadium Redox Battery Application

Park, Min-A,Shim, Joonmok,Park, Se-Kook,Jeon, Jae-Deok,Jin, Chang-Soo,Lee, Ki Bong,Shin, Kyoung-Hee Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.6

Anion exchange membranes for a vanadium redox flow battery (VRB) were prepared by pore-filling on a PE substrate with the copolymerization of vinylbenzyl chloride (VBC) and glycidyl methacrylate (GMA). The ion exchange capacity, water uptake and weight gain ratio were increased with a similar tendency up to 65% of GMA content, indicating that the monomer improved the pore-filling degree and membrane properties. The vanadium ion permeability and open-circuit voltage were also investigated. The permeability of the VG65 membrane was only $1.23{\times}10^{-7}\;cm^2\;min^{-1}$ compared to $17.9{\times}10^{-7}\;cm^2\;min^{-1}$ for Nafion 117 and $1.8{\times}10^{-7}\;cm^2\;min^{-1}$ for AMV. Consequently, a VRB single cell using the prepared membrane showed higher energy efficiency (over 80%) of up to 100 cycles compared to the commercial membranes, Nafion 117 (ca. 58%) and AMV (ca. 70%).

Chlorpropamide 2-hydroxylation is catalysed by CYP2C9 and CYP2C19 <i>in vitro</i>: chlorpropamide disposition is influenced by CYP2C9, but not by CYP2C19 genetic polymorphism

Shon, Ji-Hong,Yoon, Young-Ran,Kim, Min-Jung,Kim, Kyoung-Ah,Lim, Young-Chae,Liu, Kwang-Hyeon,Shin, Dong-Hoon,Lee, Chung Han,Cha, In-June,Shin, Jae-Gook Blackwell Science Ltd 2005 British journal of clinical pharmacology Vol.59 No.5

<P>Aims</P><P>We evaluated the involvement of cytochrome P450 (CYP) isoforms 2C9 and 2C19 in chlorpropamide 2-hydroxylation <I>in vitro</I> and in chlorpropamide disposition <I>in vivo</I>.</P><P>Methods</P><P>To identify CYP isoforms(s) that catalyse 2-hydroxylation of chlorpropamide, the incubation studies were conducted using human liver microsomes and recombinant CYP isoforms. To evaluate whether genetic polymorphisms of CYP2C9 and/or CYP2C19 influence the disposition of chlorpropamide, a single oral dose of 250 mg chlorpropamide was administered to 21 healthy subjects pregenotyped for CYP2C9 and CYP2C19.</P><P>Results</P><P>In human liver microsomal incubation studies, the formation of 2-hydroxychlorpropamide (2-OH-chlorpropamide), a major chlorpropamide metabolite in human, has been best described by a one-enzyme model with estimated <I>K</I><SUB><I>m</I></SUB> and <I>V</I><SUB>max</SUB> of 121.7 ± 19.9 µ<SMALL>M</SMALL> and 16.1 ± 5.0 pmol min<SUP>−1</SUP> mg<SUP>−1</SUP> protein, respectively. In incubation studies using human recombinant CYP isoforms, however, 2-OH-chlorpropamide was formed by both CYP2C9 and CYP2C19 with similar intrinsic clearances (CYP2C9 <I>vs.</I> CYP2C19: 0.26 <I>vs.</I> 0.22 µl min<SUP>−1</SUP> nmol<SUP>−1</SUP> protein). Formation of 2-OH-chlorpropamide in human liver microsomes was significantly inhibited by sulfaphenazole, but not by <I>S</I>-mephenytoin, ketoconazole, quinidine, or furafylline. In <I>in vivo</I> clinical trials, eight subjects with the <I>CYP2C9</I>*<I>1/</I>*<I>3</I> genotype exhibited significantly lower nonrenal clearance [*<I>1/</I>*<I>3 vs.</I>*<I>1/</I>*<I>1</I>: 1.8 ± 0.2 <I>vs.</I> 2.4 ± 0.1 ml h<SUP>−1</SUP> kg<SUP>−1</SUP>, <I>P</I> < 0.05; 95% confidence interval (CI) on the difference 0.2, 1.0] and higher metabolic ratios (of chlorpropamide/2-OH-chlorpropamide in urine: *<I>1/</I>*<I>3 vs.</I>*<I>1/</I>*<I>1</I>: 1.01 ± 0.19 <I>vs.</I> 0.56 ± 0.08, <I>P</I> < 0.05; 95% CI on the difference − 0.9, − 0.1) than did 13 subjects with <I>CYP2C9</I>*<I>1/</I>*<I>1</I> genotype. In contrast, no differences in chlorpropamide pharmacokinetics were observed for subjects with the <I>CYP2C19</I> extensive metabolizer <I>vs.</I> poor metabolizer genotypes.</P><P>Conclusions</P><P>These results suggest that chlorpropamide disposition is principally determined by CYP2C9 activity <I>in vivo</I>, although both CYP2C9 and CYP2C19 have a catalysing activity of chlorpropamide 2-hydroxylation pathway.</P>

법무부 교정국 복제(服制) 휘장디자인개발에 관한 연구 : Centered around the Badge Development of Correction Bureau Ministry of Justice

신재용,김민 한국기초조형학회 2003 기초조형학연구 Vol.4 No.1

공공기관들은 저마다의 자긍심을 표현하는 상집물을 보유하고 있으며, 제복에도 나름대로의 개성과 의미를 지닌 표지장을 부착하고 있다. 이러한 표지장은 기업이 가지고 있는 Cl와 그 성격이 비슷할 수도 있지만 그 표현에 있어서 나타나는 형태와 내포하고 있는 성격에 있어서는 다른 면을 지니고 있다. 이 논문은 표지장의 성격을 그 형태와 내용의 측면에서 분류하고 국내외 복제 표지장을 조사분석하여, 테크노 전문대학원에서 이루어진 교정공무원의 표지장 디자인 프로젝트에 대한 내용을 담고 있으며, 그에 앞서 오늘날 공공기관의 복제표지장의 근원과 일종의 권위를 나타내고 있는 서양과 동양의 상징물들을 조사하였으며, 이러한 결과에 의해 일련의 분류분석을 꾀하고, 우리나라의 실정에 맞는 표지장을 개발하기 위한 조사작업에 대하여 서술하였으며, 최종적으로 그에 따른 디자인 작업에 대한 연구를 기술하였다. Public organizations have the symbols representing their own self-esteem and the staffs have a badge on their own uniform, which has respective characteristics and meanings. Seemingly, this badge may have the characteristics similar to those of Corporate Identity(Cl) every company has. But the badge has different aspects between its external appearance and implied characters in terms of its expression. This study categorized the characters of badge in the aspect of its shape and contents, then examined and analyzed domestic or foreign uniform badges. Furthermore, the study addressed considerations on the design project of badge for educational officials, which was carried out by Graduate School of Techno Design. Before the project in advance, the study examined the origin of uniform badge available in represented contemporary public organizations and the symbolic objects around western and oriental worlds, which represent a sort of authority or dignity.

各種 Alcohol 濃度의 人蔘酒가 Mouse의 腦 및 心臟組織內 Catecholamine 含量에 미치는 影響

申萬鍊,全普權,閔本泓,李在鉉,金鐸 최신의학사 1985 最新醫學 Vol.28 No.10

중학생들의 식물들에 대한 인지도 연구

신현철,이진희,황현주,심재민 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

To investigate the understanding of middle-school students about the plants described in the middle-school textbooks, a questionnaire concerning the observation and understanding of the plants and the usefulness or kind of materials used in the lecture was developed, and distributed to a sample of middle-school students with diverse grades and locations. Although the middle-school student know plants from the textbook, and observe the plants directly or indirectly, they do not exactly know the reality of plants. Among the many reasons about this problem, the most serious aspect is the absence of the materials about the plants described in textbooks, except the textbook or references. They want to observe the plant speceimens at first, and to see the plant photos, video tape, or CD in computers.

Metallo-β-lactamase 생성 Pseudomonas aeruginosa 및 포도당 비발효그람음성간균의 검출방법에 관한 연구

신경섭,김원식,손재철,홍승복,최재운,형성민 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

연구목적: Metallo-β-lactamse (MBL)는 carbepenem를 포함하는 대부분의 β-lactam 항균제를 가수분해할 수 있으므로 MBL 생성균의 확산은 이들 균에 의한 감염의 치료에 큰 문제를 야기할 수 있다. 따라서 MBL 생성균에 의한 감염의 확산을 막기 위해 이들 균의 조기 검출이 필요하다. 저자들은 MBL 생성균을 검출할 수 있는 세 가지 방법을 PCR 방법과 비교하여 보았다. 재료 및 방법: Imipenem의 MIC가 8 μg/mL 이상인 50개의 포도당 비발효 그람음성 간균을 대상으로 EDTA-double disk synergy test (EDTA-DDS), Etest MBL, Hodge 변법을 시행하였으며 대조 검사로 IMP-1, VIM-1, VIM-2에 대한 PCR을 시행하였다. 결과: 총 50 균주 중 MBL 생성균은 Pseudomonas aeruginosa 1 균주, Alcaligenens xylosxidans 7 균주 등 8 균주(16%)가 검출되었다. 이들 균주는 모두 VIM-2 형이었으며, EDTA-DDS, Etest MBL은 PCR 방법과 100% 일치하였다. Hodge 변법은 8 MBL 생성균주 중 2 균주를 검출하지 못했다. 결론: 50 균주 중 8 균주가 MBL 생성균이었고 모두 VIM-2 형이었다. Etest MBL은 가격이 비싸 검사실에서는 EDTA-DDS가 적당할 것으로 사료된다. Purpose: Because metallo-β-lactamase (MBL) has activity to hydrolysls against most β-lactam drugs, dissemination of MBL producing bacteria may cause problems of treatment of infection by their microorganisms. Early detection or screening for MBLs will contribute to prevent further spread of resistance. Authors compared three MBL detection methods with PCR for MBLs. Materials and Methods: For 50 isolates of g1ucose nonfermentative gram negative bacilli with reduced susceptability against imipenem (MIC≥8 μg/mL), EDTA-double disk synergy (EDTA-DDS), Etest MBL and modified Hodge test were compared with PCR detection method for MBLs. Results: Among 50 isolates of glucose nonfermentative microorganisms, eight MBL producers including a Pseudomonas aeruginosa and 7 Alcaligenes xylosoxidans were detected. Those isolates were VIM-2 producer. The results of EDTA-DDS and Etest MBL completely agreed with PCR for MBL detection. Two isolates among eight MBL Producers were not detected in modified Hodge test. Conclusion: All of MBL producing bacteria were VIM-2 genotype. In conclusion, EDTA-DDS may be useful method for MBL detection in clinical laboratory due to high cost of Etest MBL strip.