혈관평활근세포에서 산화에너지대사 억제에 의한 아밀로이드전구단백질 대사의 변화

한문구,최웅,김헌식,안희열,한설희 대한치매학회 2002 Dementia and Neurocognitive Disorders Vol.1 No.1

Background: A reduction in the activity of cytochrome c oxidase(COX) has been recently identified in mitochondria from platelets and postmortem brain tissue of AD patients Sodium azide (NaN₃). a COX inhibitor, is an effective chemical agent producing energy shortage and oxidative stress both in vitro and in vivo system Furthermore it has been suggested that vascular compromise could be either directly involved AD pathogenesis or indirectly associated with triggering pathogenetic events leading to AD This study was performed to investigate amyloid precursor protein (APP) metabolism by inhibition of mitochondrial energy metabolism in cultured vascular smooth muslce cells (VSMCs) Materials and Methods: VSMCs isolated from the aorta of seven weeks old Spraque-Dawley rat were treated with NaN₃in a low concentration (100-500μM) or in a high concentration (1-100mM) Cellular proliferation and viability were determined by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenly)-2H-tetrazolium) assay Cellular APP was detected with N-terminal specific antibody 22C11. Celldeath was determined by observation of morphology and terminal deoxynucleotidyl transferase-mediated dUTP-fluorescein nick end labeling stain (TUNEL) We used ginkgo biloba extract(EGb761) and melatonin as anti-oxidants to investigate the mechanism of latered APP metabolism Results: The viability of VSMCs was increased after treatment with 1 mM and 10mM NaN₃(p<0.05) unitl 3 hr and then dimnished Many TUNEL positive cells were found in 10mM and 100mM treatment group. but were not apoptotic in nature 22C11 immunoreactivity was not changed at 3 hr, 6hr, 12hr Anti-oxidants reduced cellular proliferation (p<0.05). but did not block TUNEL positivities and did not influence the 22C11 immunoreactivity In a low concentration NaN₃ treatment group the viability of VSMCs was increased concentration dependently(p<0.05) Immunoblot with 22C11 showed the concentration dependent decrease at 145 kDa, 125 kDa. and high molecular weight range (>160kDa) TUNEL staining showed DNA fragmentations and condensations of nuclear chromatin suggesting apoptosis After treatment with anti-oxidants, the cellular proliferation was more decreased (p<0.05), and TUNEL positive cell deaths were blocked Immunoreactivities of 125 kDa (immature APP). 145 kDa (mature APP). and higher molecular weight bands were recovered below 400μM of NaN₃ Immunoreactivity of 145 kDa was recovered in 100 μM NaN₃ treated group Conclusions: The presumed mechanism of low concentration COX inhibitor is the overproduction of reactive oxygen species resulting from a depression of the mitochondiral electron transport chain. whereas potential consequence of high concentration COX inhibitor might be related to decreassion of ATP synthesis and bioenergetic impairment Reactive oxygen radicals in response to low concentration COX inhibitor alter the processing of APP in VSMCs This investigation demonstrated analtered APP metabolism as a peripheral marker of AD Therefore VSMCs treated with low concentration COX inhibitor could be concsidered as a novel in vitro model of AD.

한랭작업 근로자들의 건강위해에 관한 연구

박호추,정설미,문덕환,이종태,김대환,김정호,최재일,황용식,이용희,이채언 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.1

In order to evaluate the status of cold exposure and its health hazards of workers at cold storage workplaces and to provide basic data for effective health care, the author measured core temperature and also observed clinical symptoms and signs, past and present history, and general health examination data on 99 cold exposed workers for exposure group and 96 non-exposed workers for control group working at 2 food refrigerating companies in Pusan area from January 6, 1998 to February 24, 1998. The results were as follows: 1. There was statistically significant difference in water intake between the exposure and control group and increased urine volume, urine frequency in exposure group without statistically significant difference. 2. Past and present illness in exposure group were hypertension (18.2%), hepatopathy (8.1%), gastro-intestinal disease(7.1%), arthritis (4.0%), intervertebral disc herniation(4.0%), and so on, and hypertension, arthritis was statistically significant difference compared to control group. 3. Symptoms in exposure group were fatigue(89.9%), headache (64.6%). drowsy(63.6%), neck stiffness(59.6%), excessive food intake(59.6%). general weakness(58.6%), hunger(58.6%), numbness(54.5%), and so on, and there was statistically significant difference between the exposure and control group except fatigue, drowsy. 4. As results of clinical test abnormality rate of the systolic, diastolic blood pressure and electrocardiogram were significantly higher in exposed group than control. 5. Core temperature in exposure group was statistically significantly lower than control group and the highest statistically significant inverse correlation with the working hours and working frequency of daily mean cold storage. As above results, the author suggested that the further studies should be conducted to evaluate the health status of workers about chronic health effects in cold workplaces and to establish effective health care methods for them.

Differences in perceived parental stress between parents with very low birth weight infants and nurses in neonatal intensive care units, South Korea

Moon, Seol-Hee,Park, Ho-Ran,Kim, Dong Yeon Korean Academy of Child Health Nursing 2021 Child Health Nursing Research Vol.27 No.3

Purpose: This descriptive study compared the perceived parental stress levels between parents with very low birth weight infants (VLBWIs) and nurses in the neonatal intensive care unit (NICU). Methods: In total, 83 parents of VLBWIs and 78 NICU nurses were enrolled. Data were collected with the Parental Stress Scale (PSS) and analyzed using the t-test and analysis of variance in SAS version 9.4. Results: The average PSS score was 3.31 among parents and 3.45 among nurses. The stress score was significantly higher among nurses with children (t=2.46, p=.016) and senior nurses (t=2.12, p=.037). There was a significant difference in the stress score according to parents' education (t=3.29, p=.002) and occupation (F=3.14, p=.049) in the sights and sounds subscale. Mothers had significantly higher stress scores than fathers in the parental role alterations subscale (t=2.32, p=.023). Parental stress scores were higher than those perceived by nurses in the infant's appearance and behaviors subscale for breathing patterns (t=2.95, p=.004), followed by jerky/ restless behavior (t=2.70, p=.008). Conclusion: Nurses should provide explanations to parents of VLBWIs in order to reduce parental stress about the appearances and behavior of VLBWIs. This is more important than aspect of the NICU environment and education about parental roles.

Sub-acute toxicity study in female ICR mice following repetitive intramuscular injection of cervical cancer vaccines

Seol-Hee Moon,Du-Yeol Kim,Jung-Min Lee,Hee-Won Park,Hye-Yeong Lee,Yong-Hoon Lee,Jaesung Lee,Jiwon Jung,Min-Ju Kim,Kyoung-Baek Choi,Yu-Kyoung Oh,Young-Bong Kim,Sujeong Kim,Seung Min Oh 환경독성보건학회 2014 환경독성보건학회지 Vol.29 No.-

Objectives : The sub-acute toxic effects following repetitive intramuscular injection of two cervical cancer vaccines newly developed against human papillomaviruse (HPV)16/58/18 and HPV16 were investigated in female ICR (CrljOri: CD1) mice, and the no-observedadverse-effect-level (NOAEL) of the cervical cancer vaccines was estimated. Methods : Female ICR mice (n=15 in each group) were exposed to a 1:1 mixture of two cervical cancer vaccines by repetitive intramuscular injection (once a week, 5 times) for 5 weeks. Mortality, body weight, organ weight, hematological/biochemical parameters, and histopathological effects were examined at different concentrations (0, 1×10<SUP>8</SUP>, 5×10<SUP>8</SUP>, and 2.5×10<SUP>9</SUP> copies/animal) of the cervical cancer vaccines. Results : The cervical cancer vaccines did not show toxic responses for body weight, absolute/relative organ weight, hematological/biochemical parameters, or histopathological parameters. Conclusions Female ICR mice exposed to vaccines for cervical cancer did not show any toxic response. We suggest that a NOAEL of the vaccine following repetitive intramuscular injection for 5 weeks is >2.5×10<SUP>9</SUP> copies/animal.

상업지역 가로경관의 색채조화를 위한 연구

문설희(Moon Seol-Hee),성인수(Seong In-Soo) 대한건축학회 2007 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.27 No.1

One of the most important factors affecting the quality of the streetscape is the color conditioning. The color of streetscape can make various image about the street. Currently, the color of streetscape is used planlessly. As a result of the planless color conditioning, streetscape becames confused. The purpose of this study is to search for the best way to make the color condition of streetscape harmonious. For this study we selected the free zone of cars in Ulsan which was designated as specialize street.

형제에서 발생한 항-M 항체에 의한 신생아 용혈성 질환

안설희 ( Seol Hee Ahn ),이연경 ( Yeon Kyung Lee ),고선영 ( Sun Young Ko ),신손문 ( Son Moon Shin ),조동희 ( Dong Hee Cho ) 대한주산의학회 2015 Perinatology Vol.26 No.3

Hemolytic disease of the newborn (HDN) caused by anti-M is rare and clinical manifestations are variable ranging from mild anemia and hyperbilirubinemia to hydrops fetalis and intrauterine fetal death. There were few reports of HDN caused by anti-M in Korea but no case in siblings. We experienced a case of 2 male siblings, both of whom had anti-M induced HDN and prolonged anemia persisted for over a month. We report this case with a brief review of literature. This report documents the first case of HDN caused by anti-M in siblings in Korea.

SUMO-specific protease SUSP4 positively regulates p53 by promoting Mdm2 self-ubiquitination

Lee, Moon Hee,Lee, Sung Won,Lee, Eun Joo,Choi, Soo Joon,Chung, Sung Soo,Lee, Jae Il,Cho, Joong Myung,Seol, Jae Hong,Baek, Sung Hee,Kim, Keun Il,Chiba, Tomoki,Tanaka, Keiji,Bang, Ok Sun,Chung, Chin Ha Nature Publishing Group 2006 Nature cell biology Vol.8 No.12

The p53 tumour suppressor has a key role in the control of cell growth and differentiation, and in the maintenance of genome integrity. p53 is kept labile under normal conditions, but in response to stresses, such as DNA damage, it accumulates in the nucleus for induction of cell-cycle arrest, DNA repair or apoptosis. Mdm2 is an ubiquitin ligase that promotes p53 ubiquitination and degradation. Mdm2 is also self-ubiquitinated and degraded. Here, we identified a novel cascade for the increase in p53 level in response to DNA damage. A new SUMO-specific protease, SUSP4, removed SUMO-1 from Mdm2 and this desumoylation led to promotion of Mdm2 self-ubiquitination, resulting in p53 stabilization. Moreover, SUSP4 competed with p53 for binding to Mdm2, also resulting in p53 stabilization. Overexpression of SUSP4 inhibited cell growth, whereas knockdown of susp4 by RNA interference (RNAi) promoted of cell growth. UV damage induced SUSP4 expression, leading to an increase in p53 levels in parallel with a decrease in Mdm2 levels. These findings establish a new mechanism for the elevation of cellular p53 levels in response to UV damage.

일부 특수학교 장애인의 치아우식증과 식생활실천지침 이행정도의 관련성

문희정 ( Hee Jung Moon ),김설악 ( Seol Ak Kim ),박희정 ( Hee Jung Park ) 한국치위생학회(구 한국치위생교육학회) 2012 한국치위생학회지 Vol.12 No.6

Objectives: The purpose of our study was to examine the relationship of oral health status such as dental caries and the performance of dietary action guides with six items. Methods: Oral examination and self-administrated questionnaire were conducted on 87 disabled students, aged 6-19 who were attending special school in the area of the metropolitan Seoul. Two logistic regression models to estimate odds ratios and 95% confidence intervals were used to investigate the association dental caries experience and the performance of dietary action guides after controlling for potential confounders. Results: The proportion of study subjects with caries were 37.9%, 26.9%(male) and 54.3%(female), respectively(p<0.05). There was significantly difference the subjects with caries and perceived oral health status(p<0.01). Among the dietary action guides items, skipping breakfast and not avoiding waste food were significantly higher in the proportion of subjects` caries(p<0.05, p<0.01, respectively). The avoiding waste food groups had more possibility of having caries significantly(p<0.05), even after controlling gender, and perceived oral health status. Conclusions: These results indicate that oral health promotion policy with dietary action guides should be strengthened for support of disabled student`s dental health.

급성 A형간염 이환기간 중 발생한 횡문근융해증 1예

안서희 ( Soe Hee Ann ),안건희 ( Gun Hee An ),이수연 ( Su Yeon Lee ),옥주현 ( Ju Hyun Oak ),문형일 ( Hyung Il Moon ),문설경 ( Seol Kyung Moon ),한남익 ( Nam Ik Han ),이영석 ( Young Sok Lee ) 대한간학회 2009 Clinical and Molecular Hepatology(대한간학회지) Vol.15 No.1

Caffeine prevents human prion protein-mediated neurotoxicity through the induction of autophagy

MOON, JI-HONG,LEE, JU-HEE,PARK, JIN-YOUNG,KIM, SUNG-WOOK,LEE, YOU-JIN,KANG, SEOG-JIN,SEOL, JAE-WON,AHN, DONG-CHOON,PARK, SANG-YOUEL Spandidos Publications 2014 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.34 No.2

<P>The human prion protein (PrP) fragment PrP(106?126) possesses the majority of the pathogenic properties associated with the infectious scrapie isoform of PrP, known as PrPSc. The accumulation of PrPSc in the brain of humans and animals affects the central nervous system. Recent epidemiological studies have suggested that caffeine, one of the major components of coffee, exerts protective effects against the development of neurodegeneration. However, the protective effects of caffeine against prion disease have not been reported to date. In this study, we therefore investigated the effects of caffeine on PrP-mediated neurotoxicity. The protein expression of the autophagosomal marker, LC3-II, was increased by caffeine in a dose-dependent manner, and the autophagy induced by caffeine protected the neuronal cells against PrP(106?126)?induced cell death. On the contrary, the downregulation of LC3-II using the autophagy inhibitors, 3-methyladenine (3-μα) and wortmannin, prevented the caffeine-mediated neuroprotective effects. To the best of our knowledge, the present study provides the first evidence that treatment with caffeine protects human neuronal cells against prion?mediated neurotoxicity and these neuroprotective effects are mediated by caffeine-induced autophagy signals. Our data suggest that treatment with caffeine may be a novel therapeutic strategy for prion peptide?induced apoptosis.</P>