Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

배차계획을 위한 대화형 의사결정지원시스템

박양병,홍성철 한국경영과학회 1998 經營 科學 Vol.15 No.2

Truck dispatching is one of the most commonly occurring problems of transport management. We developed an interactive decision support system named IDSSTD, for the truck dispatching problem where two conflicting objectives are treated and travel speed varies depending on the passing areas and time of day. The IDSSTD aids the decision-making process by allowing the user to interact directly the database, to direct data to a decision model, and to portray results in a convenient form. The IDSSTD is consisted of two major interactive phases. The pre-scheduling interactive phase is to reduce the complexity of a given problem before applying the BC-saving heuristic algorithm and the post-scheduling interactive phase is to improve practically the algorithmic solution. The IDSSTD has the capabilities of its own manipulation(analysis and recommendation) and diverse graphic features in order to facilitate a user's interaction.

ASEA-SKF 래들용 고알루미나 내화재의 용손성향

박홍채,이윤복,양태영,장윤식,오기동 釜山大學校生産技術硏究所 1997 生産技術硏究所論文集 Vol.53 No.-

ASEA-SKF 래들에 사용된 고알루미나 내화물의 광물 및 화학적 변화를 화학분석, 현미결관찰, X-선회절분석으로 조사하였다. 미사용 벽돌의 주요 광물상은 α-Al₂O₃, mullite, tridymite이었다. 가동면에서 SiO₂결정상이 소실되었으며 mullite와 α-Al₂O₃가 다소 감소하였고 2FeO·SiO₂와 hercynite, FeO, Cr₂O₃상이 생성되었다. 침식율은 강종에 따라 다소간 차이가 있었으며 용강 SF-45와 60분간 반응시켰을때 SK36벽돌의 침식율은 75.9%인 반면에 SK38벽돌의 침식율은 20.47%이었다. 대부분의 용손벽돌에서 가동면으로부터 내부로 감에 따라 열적 및 화학적 스폴링으로 인하여 미세균열이 존재하였다. Mineral and chemical changes of alumina refractories used in ASEA-SKF ladle were examined by chemical analysis, microscopy and X-ray diffraction method. Main minerals in unused high alumina runner bricks were α-Al₂O₃, mullite and tridymite. In working face of high alumina runner bricks, SiO₂phase was not observed, mullite and α-Al₂O₃phases more or less decreased. On the other hand, 2FeO·SiO₂, hercynite, FeO and Cr₂O₃phases were formed. Th corrosion of high alumina runner bricks showed different behavior by using various types of iron melts. Expecially, corrosivites of SK36 bricks and SK38 bricks, which immersed in SF-45 Iron melts for 60min, were 75.9% and 20.47%, respectively. Microcracks in high alumina runner bricks increased from the working face to the inside due to thermal and chemical spalling.

무지개 松魚의 遺傳 育種學的 硏究 : Ⅳ.血淸成分 水準의 年中 變化 Ⅳ.Annual Changes of Serum Components Levels

朴弘陽,尹鐘萬,金桂雄 건국대학교 동물자원연구센터 1991 動物資源硏究誌 Vol.16 No.-

본 연구는 무지개 松魚 암컷 3년생 120마리에 대하여 1989년 3월부터 1990년 2월까지 계절 및 人工産卵前後에 따른 血淸成分 含量 水準을 측정하였고, 각 血淸 成分間의 상관관계를 조사하였다. 장차 무지개 松魚의 繁殖效率 增進에 필요한 遺傳育種學的 基礎 資料를 얻기 위하여 실시되었다. 본 연구에서 얻어진 결과를 요약하면 다음과 같다. 1) 血淸中 albumin, calcium, glucose, total protein, alkalin phosphatase, SGOT 및 phosphorus의 함량은 産卵期中에 높은 수준이었으나, cholesterol, creatinine 및 triglycerides의 含量은 卵黃形成期中에 비교적 높은 수준을 유지 하였다. 2) 人工産卵展, 産卵後 및 過熟時期에 따른 血淸中 albumin, calcium, cholesterol, creatinine, total protein, triglycerides, alkaline phosphatase 및 phosphorus의 含量은 人工産卵前에 비교적 높은 수준을 유지한 반면에, BUN, glucose, SGOT 및 SGPT의 含量은 過熟時期에 높은 수준이었다. 그리고 A/G 比率은 非産卵期인 6월, 7월 및 8월에 最高水準을 유지하다가 産卵時期直前부터 감소하여 산란시기 동안에 낮은 수준으로 계속 유지되었다. 3) 産卵期中 albumin수준과 calcium(r=-0.793)간에는 부(-)의 상관이었으나, 그 밖의 다른 성분간에는 有意性이 인정되는 정(+)의 상관이 존재하였다. BUN 含量水準과 alkaline phosphatase(r=0.790), SGOT(r=0.881) 및 SGPT(r=0.902)와는 正(+)의 高度相關이 존재하였다. Glucose수준과 모든 다른 성분간에는 負(-)의 相關關係였으마, cholesterol수준과 그 밖의 다른 성분간에는 正(+)의 相關이 존재하였다. Glucose수준과 total protein(r=0.856), triglycerides(r=0.973), alkaline phosphatase(r=0.738), SGOT(r=0.711) 및 SGPT(r=0.738), 및 SGPT(r=0.657)間에는 正(+)의 高度相關을 보였으며, creatinine 수준과 total protein(r=0.672)에도 正(+)의 상관관계가 존재하였다. Total protein과 triglycerides(r=0.911)와는 正(+)의 高度相關이었으며, alkaline phosphatase 水準과 SGOT(r=0.936) 및 SGPT(r=0.926)間은 正(+)의 高度相關關係가 있다. 또한 SGOT와 SGPT(r=0.995)와는 매우 높은 정(+)의 상관관계가 존재하였다. 4) 産卵期中 호르몬과 血淸成分間의 상관관계에 있어서 血淸中 LH 濃度와 BUN(r=0.747)과는 유의한 負(-)의 相關이었으나, creatinine(r=0.887) 및 total protein(r=0.688)과는 正(+)의 高度相關이 존재하였다. FSH濃度와 creatinine(r=0.831) 및 total protein(r=0.709)간에는 正(+)의 상관관계가 있었다. Estradion-17β와 creatinine(r=0.899), total protein(r=0.899), total protein(r=0.859) 및 trigpycerides(r=0.673)간에 비교적 높은 正(+)의 상관이었으나, progesterone 濃度과 BUN(r=-0.606)간에는 負(-)의 상관관계가 존재하였다. This study was carried out to investigate serum components levels(12 components) obtained from 120 of 3-year-old female rainbow trout(Oncorhynchus mykiss). All data were collected and analysed monthly from March in 1989 to February in 1990. Serum protein components were assayed by blood analyzer. The results obtained in this study were as follows; 1. The contents of serum albumin ,calcium, glucose, total protein, alkalkine phosphatase, SGOT, and phosphorus increased during spawning period, but those of serum cholesterol, creatinine, and triglycerides maintained relatively high levels during vitellogenetic period. 2. The contents of serum albumin, calcium, cholesterol, creatinine, total protein, triglycerides, alkaline phosphatase and phosphorus prior to handstripping were relatively high, while those of serum BUN, glucose, SGOT and SGPT were high in over-ripe stage. 3. The correlation between serum albumin and calcium during correlations of albumin and other contents were significantly positive. Those of serum BUN with alkaline phosphatase(r=0.790), SGOT(r=0.881) and SGPT(r=0.902) were highly positive. The content of calcium was highly correlated with various serum contents, and that of cholesterol was a signigicantly correlated with other serum contents. The correlations of serum glucose with total protein(r=0.856), triglycerides(r=0.973), alkaline phosphatase(r=0.738), SGOT(r=0.711) and SGPT(r=0.657) were significantly high. The correlation coefficients between creatinine and total protein, between total protein and triglycerides, and between SGOT and SGPT were significantly high. In addition, those of serum alkaline phosphatase with SGOT and SGPT were also significantly high. 4. The correlation of serum LH with BUN during spawning period was highly negative(r=-0.793), while the correlations of LH with creatinie and total protein were high(r=0.887 and 0.688, respectively). Similarly, the relationships of serum FSH with creatinine(r=0.831) and total protein(r=0.709) were significantly highly positive. The concentration of estradiol-17βwas highly correlated with creatinie, total protein, and triglycerides. But the relation of serum progesterone with BUM was significantly negative(r=-0.606).

전신마취중 에피네프린사용으로 발생되는 부정맥

박선규,진홍용,양홍석 중앙대부설한국의과학연구소 1985 한국의과학 Vol.17 No.3

Mulligan 치료법과 고유수용성 신경근 촉진법이 동결견 환자의 치료에 미친 효과

양정애,윤홍일,박현식,신영일,전범수 대한정형도수치료학회 2006 대한정형도수물리치료학회지 Vol.12 No.1

The purpose of this study was to compare the effects of Mulligan method and PNF method on the pain and limitation of range of motion in patients with frozen shoulder. The subjects of this study were 20 patients, 10(50%) males and 10(50%) females. They visited clinic for physical treatment within 6 months after onset of shoulder pain and limitation of range of motion with frozen shoulder. One group was applied with Mulligan method and other group was with PNF method . The patient were treated 5 times session weekly for 6 weeks from March 4th, 2002. And each treatment session was 15min. with physiotherapy. The pain was measured by visual analogue scale (VAS) and rage of motion (ROM) of flexion, abduction, external rotation, internal rotation were measured by goniometer. The data was analysed by paired T-test and independent T-test. The results of this study were summarized as follow : 1. The ROM of Mulligan method group increase in after treatment in comparison with ROM in before treatment, it is significant increase. Although the VAS of MMG decrease in before than after treatment, it is significant difference. 2. There is significant difference in before and after treatment of ROM of shoulder flexion, abduction, internal rotation, external rotation between PNF method group. The VAS of PMG is decreasing in before than after treatment, it is significant difference. 3. There is significant difference in before movement and after movement ROM of flexion, abduction, internal rotation, external rotation and VAS between Mulligan method session and PNF method session then the scale which measured by Mulligan method was more increased than that of PNF method. The results showed that both Mulligan method and PNF method were effective in pain reduce and ROM increase, but Mulligan method was superior to PNF method in ROM increase and pain reduce.

일간지 생산-배송계획 문제를 위한 혼성유전알고리듬 해법

박양병,홍성철 한국경영과학회 2003 한국경영과학회 학술대회논문집 Vol.- No.1(1)

많은 기업들은 그들의 생산과 배송 기능을 분리하여 각각의 최적화를 꾀하고 있는데, 이러한 접근방법에 의해 이룰 수 있는 절약은 제한적일 수밖에 없다. 최근의 공급사슬경영(SCM)에서는 이들 두 기능의 관련 비용을 총체적 관점에서 잘 조절하면서 동시에 통합 분석하는 것이 더 중요하다고 인식되고 있다. 본 논문에서는 일간지의 생산-배송계획 문제를 다룬다. 일간지는 재고로 유지될 수 없으며, 고객이 정한 시간보다 늦게 배송되면 큰 손실을 야기하는 일종의 단일기간 재고품목이다. 저자들은 일간지 생산-배송의 통합계획을 위한 혼성유전알고리듬 해법을 개발하고 예제를 이용하여 Hurter and Buer의 순차적 분리계획 해법과 성능을 비교한다. 계산실험 결과, 개발된 해법의 성능은 효과적인 것으로 나타난다.

폐결핵 환자의 우울 상태

홍정표,최선희,조만종,이성주,박종,김기순,김양옥 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.1

The main purpose of this study is to identify and evaluate the depressive states for the patients with chronic pulmonary tuberculosis. A questionnaire(Self-rating Depression Scale, SDS) survey was performed toward 57 pulmonary tuberculosis patients with initial treatment who started chemotherapy and 53 pulmonary tuberculosis patients with retreatment, admitted to one tuberculosis-centered hospital and two general hospital. Fifty three healthy persons as control group were investigated with the same questionnaire for comparison. The survey was performed from July to August 1996. The results are as follws : 1. The patients had significantly high scores in the depression than control and the patient with retreatment showed significantly high scores in the depression than the patient with initia1treatment(P<0.01). 2. The depressive levels of the patients were associated with duration of admission and the expectancy of recovery (P<0.05). 3. The percentage of the subjects with 40 or more scores tentatively classifiable as psychologically depressed was 67.1% of the total subjects, 48.4% of the control groups, 75.0% of the patient with initial treatment and 84.1% of the patient with retreatment. Because patient with pulmonary tuberculosis with initial treatment showed higher depression scores than normal persons, and patients with retreatment due to failure of initial treatment showed higher depression scores than initial treatment, it is necessary to give attention to depression of the patients during treatment of pulmonary tuberculosis.

교류전해에칭용 에틸렌글리콜의 알루미늄박판의 부식억제 효과

박성민, 양정진, 김홍일, 김한주, 김성한, 신진식, 박수길 충북대학교 건설기술연구소 2008 충북대학교 건설기술연구소 논문집 Vol.27 No.1

In this study, additions of aluminium foil eching characteristics were compared with sulfuric acid for capacitor. sulfuric acid and ethylene glycol is used as addition, characteristics of funtional group from addition were guessed by comparing of aluminium foil eching characteristics. When sulfuric acid was added in HCI etching solution, corrosion of aluminum metal was inhibited by the chemical adsorption of sulfate ion, when ethylen glycol was added in HCI etching solution, corrosion inhibited by the chemical adsorption of OH­^ion.

우묵사스레피(Eurya emarginata)의 플라보노이드 화합물의 분리 및 동정

양홍철,박수영,강희경,이남호 제주대학교 생명과학연구소 2001 제주생명과학연구 Vol.4 No.-

The extracts of Eurya emarginata showed inhibitory effect on the growth of HL-60 leukemia cells. So, 80% methanol(MeOH) extract of its leaves was partitioned to hexane, ethyl acetate, n-butanol and residual water fractions. The ethyl acetate fraction, which showed desirable activities, was further purified using SiO2 column chromatography(CC) followed by reverse phase silica gel CC, then another normal CC to give fraction 4-1-8 as a pure compound. The compound 4-1-8 was analyzed by NMR spectrometry using 1H, 13C, DEPT, HMQC and HMBC spectra, and finally proved to be well-known flavono glycoside, Quercitrin.