토양으로부터 分離한 Bacteriophage의 특성

박현균,김도경,박경진,전홍성,박종필,김종승,김성준 조선대학교 부설 유전생물공학연구소 1992 유전생물공학연구지 Vol.2 No.-

환경 미생물 분야와 산업미생물 분야 및 분자생물학 분야에서 주목을 받고 있고, 많은 연구가 된 새로운 E. coli의 bacteriophage를 토양으로 부터 분리해서, 그 물리적인 특성과 핵산을 밝혀내, phage상호간의 관련성을 검토하여 보았다. 그 결과를 요약하면 다음과 같다. 1. 토양으로 부터 분리한 phage는 숙주균에 따라 SJ-1, SJ-2, SJ-3, SJ-4, SJ-5, SJ-6, SJ-7이라 명명하였다. SJ phage들은 높은 숙주특이성을 나타내 phage SJ-2, SJ一7은 단일종 숙주균에만 감염이 되었고, SJ-1, SJ-3, SJ-4, SJ-5, SJ-6은 다중독성을 나타내었다. 2. 7종류의 SJ-phage는 본 실험조건하에서는 모두 virulent phage임이 밝혀졌다. 모든 용균반의 형태는 중앙이 투명한 둥근 모양이었으며 주변에 무리가 있는 것과 없는것 두가지 형태를 나타냈다. 용균반의 크기는 1-5mm이었으며, SJ-5phage의 용균반이 5mm로서 가장 컸고 SJ-7 phage가 가장 적었다. 숙주균은 OD_600 0.3-0.5상태에서 가장 감염되기가 좋았다. 그리고 상층배지의 한천 농도와 양은 0.5%와 3ml이 가장 좋은 조건이었다. 3. 일단 증식 실험의 결과를 보면 SJ-phage는 잠복기가 15-50분이었고 평균 phage방출수는 190-250이었다. SJ-6과 SJ-7이 잠복기가 15분으로 가장 짧았고 SJ-4가 50분으로 가장 길었다. 평균 방출수는 SJ-3과 SJ-5가 250정도로 많았으며 SJ-1과 SJ-2는 약 190정도였다. 4. SJ-phage는 PD buffer에서 안정하였고, magnesium ion 5×10^-3M의 첨가는 phage에게 큰 안정성을 주었다. SJ-phage는 pH6과 pH9사이에서 안정하였으며 그 pH는 숙주균의 최적 pH였다. SJ-phage의 온도 불활성화는 40 ℃이상에서 불활성화되기 시작하여 60 ℃이상에서는 완전히 불활성화 되었다. 자외선 조사에 의한 불활성화에서 SJ-phage는 120초 이후에 완전히 불활성화 되었다. All phages used in this experiment were isolated from soil of the surburbs of Chun-Nam, and they designated as phage SJ-1, SJ-2, SJ-3, SJ-4, SJ-5, SJ-6, SJ-7. The hosts were JM105, JM109, K802, LE392, MC1000, MV1184 and PSM CC, respectively. These phages were virulent under the experimental conditions. They produced plaques with dear and round without halo. The size of plaques was 1-5mm in diameter. SJ-phages were stable in 5×10^-3M Mg^2+. Thermostability experiments indicated that SJ-phages were stable at 37℃. Optimal pH of the phages were 6-9. Exposure of the phages to U.V. for 120 seconds resulted in complete inactivation. In one-step growth experiments, the latent period at 37 ℃ was about 15-50 min and the average burst see was 190-250. Agarose gel electrophoresis of the phage DNA showed single band of high molecular weight from SJ-1 to SJ-7 phages

일산화탄소 폭로후 고압산소 재폭로시 흰쥐 신장 및 심장의 SOD 활성 변동에 관한 연구

전영희,백두진,황세진,박준숙,정호삼 한양대학교 의과대학 1997 한양의대 학술지 Vol.17 No.1

Carbon monoxide is generated from the incomplete combustion of carbon-containing materials. Carbon monoxide cause muscle necrosis leading to myoglobinuria and acute renal failure. The hyperbaric oxygen exposure is increased production of active free radicals. Free radicals which promoted peroxidation of unsaturated fatty acids may destroy essential cellular components and itself cytotoxic materials. Superoxide dismutase, one of the protective mechanisms against tissue damage, catalyzed the dismutation of superoxide anion to less toxic product. The author performs the present study to investigate on Mn-SOD and Cu, Zn-SOD immunoreactivities in kidney and heart carbon monoxide (CO) and hyperbaric oxygen (HBO) exposure. The animals were divided CO exposure group, air release after CO exposure group, HBO exposure group and, CO and HBO exposure group. The specimen were sectioned in 16㎛ with cryostate. The Mn-SOD and CuZn-SOD immunohistochemistry was performed in renal corpuscle, proximal and distal convoluted tubules of kidney and, in sarcoplasm and sarcolemma of heart muscle. The results obtained were as follows: 1. The immunoreactivity of Mn-SOD were increased in renal corpuscles and distal convoluted tubules, were similar to control group in proximal convoluted tubules. 2. The immunoreactivity of Cu, Zn-SOD were increased than that of control group in renal corpuscles and distal convoluted tubules, were same that of the control group in proximal convouted tubules. 3. The immunoreactivity of Mn-SOD were increased at all experimental group of sarcoplasms of heart muscle. 4. The immunoreactivity of Cu,Zn-SOD were increased than that of control group in sarcolemma of heart muscle. These results suggest that the immunoreactivity of SOD after CO or HBO exposure is greatly increased in kidney and heart.

複合 作動液을 갖는 回轉型 히트파이프의 傳熱特性

박춘근,전철호,서해성 동아대학교 공과대학 부설 한국자원개발연구소 1991 硏究報告 Vol.15 No.1

Heat transfer characteristics of internally triangler finned rotating heat pipe with the water-ethanol mixture as working fluid have been numerically and experimentally studied. The numerical and experimental results showed good agreement and when the ethanol content change, application limit of rotating heat pipe could change. By increasing rpm or vapour pressure of rotating heat pipe, heat transfer rate was increased slightly but when ethanol content increase, heat transfer rate was decreased. In case of pure ethanol for working fluid, heat transfer was about 0.8 compared with distilled water. When ethanol content was about 0.2 heat transfer rate and applicable temperature was profitably appeared. When ethanol content was about 0.2, heat transfer rate and applicable temperature difference of rotating heat pipe.

계층적인 탐색점 배치와 화소 간축을 이용한 움직임 추정 기법

박준호,류명춘,김준한,김신희 경운대학교 산업기술연구소 1999 産業技術硏究論文誌 Vol.1 No.1(B)

In motion estimation of moving pictures compression, fast block matching method using hierarchical search point displace and the pixel decimation is supposed. The method is based on TSS(Three step Search) method and pixel decimation. This method improves image quality and local minima by increasing search points, but operation counts that resulted from increasing search points are reduced by the pixel decimation method. So, the method can improve image quality and operation counts are nearly the same with TSS. MP@ML video is simulated by this method, which shows good results compared with TSS.

치과용 고무인상재의 영구변형 및 압축시 변형률

전창주,김준철,정석민,박영준 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.1

This study investigated the permanent deformation of dental rubber impression materials in order to evaluate their degree of recovery from displacement during removal through undercut area. We also investigated the stain in compression in order to evaluate the materials' flexibility during removal from the month, or during removal of the cast from the impression. Additional silicone, polyether, and polysulfied rubber impression materials were tested for their properties of permanent deformation and strain in compression according to the testing methods of the ADA specificaltion no. 19. 1.The permanent deformation values were as follows: polysulfide (2.25%), polyether (2.14%), and additional silicone (0.28∼1.21%, 2.38% for Perfect F). Examix, Express and Provil, which are additional silicone rubbers, showed lower permanent deformation values of 0.29%, 0.28% and 0.34%, respectively. Perfect F, although it is an additional silicone rubber, showed the highest permanent deformation value (2.38%) within the tested materials. 2.The strain in compression values were as follows: polysulfide (10.63%), additional silicone (2.88∼10.09%), and polyether (2.38%). The additional silicone rubber samples showed various values. Contrary to our expectations, it is also notable that Vinylate, which is used when taking an one-step impression, had higher stain in compression value (10.09%) than the light body impression materials even though it is classified as a heavy body material. Express (a.s.) had a low strain in compression (2.88%) which is similar to that (2.83%) of Impregum F (p.e.). In light body rubber materials, Permlastic had the highest value (p.s. 10.63%), followed by Perfect F (a.s. 7.38%), Correct VPS (a.s. 7.13%), Examix (a.s. 6.04%), Provil (a.s. 3.82%) and Express (a.s. 2.88%). Although the tested materials showed various values, all of them satisfied the requirements of A.D.A. Specification No. 19, which require that permanent deformation be less than 2.5%, and strain in compression be between 2∼20%.

學校建築의 設計計劃에 관한 硏究 (Ⅱ) : 學校施設關係令 및 施設水準에 관한 考察 Servey of School Facility and Standard

朴春根,兪吉濬 동아대학교 공과대학 부설 한국자원개발연구소 1986 硏究報告 Vol.10 No.1

This study is on the point at issue of primary and middle school building planning to the betterment of teaching environment. The conclusions are as follows ; 1. It need to establishment of definition about the land areas occupied by school and improvement about the standard of land areas. 2. It need to flexibility of the school building design and regulation about the standard of building areas.

서울시 주택내 휘발성 유기화합물 공기오염농도의 계절변화에 관한 연구

전정윤,박준석,손장열 대한건축학회 2005 대한건축학회논문집 Vol.21 No.3

For the purpose of examining about seasonal change in concentration of indoor organic compounds in Seoul, 96 houses in the winter and 49 houses in the summer were investigated. Indoor organic compounds concentration were measured and survey was performed by questionnaire. Indoor organic compounds were monitored with two diffusive samplers. One was a cartridge treated with 2,4-dinitrophenylhydrazine for aldehydes sampling. The other was a passive charcoal tube for VOCs sampling. Two samplers were simultaneously exposed in living room during 24 hours. Aldehydes extracted with 2㎖ acetonitrile from the cartridge were analyzed by HPLC equipped with an UV diode array detector. VOCs were extracted with 2㎖ carbon disulfide from the charcoal tube by shaking and then analyzed by chromatograph and mass selective detector. The main Results were as follows. (1) Both of Formaldehyde and VOCs concentration in the summer are lower than that in the winter. This is because of increasing ventilation rate according to the opening of windows in the summer. (2) Formaldehyde, toluene and ethylbenzene were indicated as the major indoor volatile organic compounds in Seoul Residence. (3) There were 10 times of deference in concentration between non-occupied new house and that of post-occupied. (4) There was no significant relation between house type and indoor concentration.

태양열 집열기용 평판 히이트파이프의 전열예측

박춘근,전철호,장영석 동아대학교 공과대학 부설 한국자원개발연구소 1994 硏究報告 Vol.18 No.2

The purpose of the research is to study the prediction of heat transfer on flat heat pipe applid to solar energy collector by the numerical study. Numerical analysis was performed by finite element method for the prediction of heat transfer on flat transfer on flat heat pipe. A comparison between the experimental and the numerical results show qualitatively agreement and it was able to predict the performence of flat heat pipe collector. The Biot number, (h_(ev)l)/Ks in order to know the effect of heat transfer on flat heat pipe was show to decrease by increas of heat transfer coefficient in vapor region, and with higher conductivity of container, Ks.

內部熱源을 갖는 直方體의 傳熱特性에 관한 硏究

朴春根,全哲昊,徐海成 동아대학교 공과대학 부설 한국자원개발연구소 1984 硏究報告 Vol.8 No.2

A numerical and experimental study have been performed on the heat transfer in rectangular solid owned heat source. The effects of thermal conductivity k, heat transfer coefficient h, temperature of surrounding fluid To and the rate of heat flow Q were studied numerically by F. E. M. The experiment were studied by given rectangular solid. The results of F. E. M. and experimental are as follows. 1) The F. E. M. to calculate the temperature distribution in rectangular solid showed good agreement with experimental. 2) Thermal conductivity influenced strongly temperature distribution in the solid but heat transfer cofficient influenced only surface. 3) Temperature gradient in the solid was unconcerned by temperature of surrounding fluid and rate of heat flow but temperature in the solid changed at a fixed rate.

工具材料의 高速에 있어서의 摩擦摩耗

全泰玉,朴春根 동아대학교 환경문제연구소 1981 硏究報告 Vol.5 No.1

This study has analyzed examined an experiment on the frictional wear of tool materials at high speed. The results of experiment are as follows. 1. With the increase in the frictional velocity and the contact pressure, the decrease of the frictional coefficient is influenced more by the softness of the frictional surface layer than by oxide. 2. Specific wear rate is influenced more by the frictional velocity than by the contact pressure. 3. The character of the specific wear rate and the contact pressure of each test piece is similar to one another.