소아에서 나타난 비구개관낭의 치험례

모정희,정현구,조태식,김효석,박성규,이난영 조선대학교 구강생물학연구소 2002 口腔生物學硏究 Vol.26 No.2

The nasopalatine duct cyst is the most common non-odontogenic cyst in the oral area and is thought to be originated from the epithelial remnants of the nasopalatine duct. The nasopalatine duct cyst have been mentioned to be an overall incidence of 1.7-11.9% of all jaw cyst. More males than females are affected by that. It could occur all around the age, and especially in their 40th and 60th it happens the most and in childhood it is very rare. Proper treatment for nasopalatine duct cyst is enucleation. In the case of very large cysts, it is likely to be a risk of naso-oral or antral-oral fistulas or of devitalization of teeth, marsupialization is indicated. The purpose of case report is that a nasopalatine duct cyst in is very rare in childhood. A 9-year-old boy was referred to the chosun university pedodontics clinic that this child who has anterior palatal swelling is in routine check. The size of the cyst was so big that the marsupialization was committed and obturator was put. This patient is on the continuous observation. we treated the nasopalatine duct cyst with obturator.

Smad에 의한 alkaline phosphatase 유전자의 발현 조절기전

김난진,류현모,김현정,김영진,남순현 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.2

본 실험은 탁월한 골유도능으로 관심의 대상이 되고 있는 RMP의 세포내 신호 전달자로 알려진 Smad 1과 Smad 5가 조 골세포 초기 분화표지인자인 ALP 유전자의 발현에 미치는 영향 및 그 조절기전을 알아보고자 하였다. BMP 처리 없이도 Smad에 의해 ALP가 발현되는가를 알아보기 위해 Smad 1과 Smad 5가 각각 stably transfection된 C2C12 세포를 3일간 배양후 histochemical assay를 하였고, Smad 1과 Smad 5의 expression vector와 ALP promoter vector를 transient co-transfectiongksgn ALP promoter activity를 측정하였다. Smad에 의한 BMP의 효과를 알아보기 위해서 100ng/ml의 BMP-2를 처리한 군과 처리하지 않은 군으로 나누어 세포를 배양한후 ALP 유전자의 발현을 northern blot analysis로 확인하였다. Smad가 ALP 유전자의 발현을 직접적으로 조절하는가를 알아보기 위여서는 단백질 합성억제제인 cycloheximide를 전처리하여 ALP 유전자의 발현을 northern blot analysis하였다. 이상의 실험결과 다음과 같은 결론을 얻었다. ·Smad 1과 Smad 5가 과발현된 세포에서는 BMP 처리없이도 ALP가 발현된다. ·Smad 1과 Smad 5가 과발현된 세포에서 RMP 처리후 ALP 발현 증가율이 대조군 보다 현저히 높게 나타나 Smad가 BMP 효과를 증가시킨다는 것을 알수있다. ·Smad는 새로운 단백질의 합성을 통해 ALP 유전자를 발현시킨다. Bone morphogenetic proteins(BMPs), members of the transforming growth factor β(TGF-β) superfamily were first identified as the factors that induce ectopic bone formation in vivo, when implanted into muscular tissue. Especially BMP-2 inhibits terminal differentiation of C2C12 myoblasts and converts them into osteoblast lineage cells. In the molecular mechanism of the signal transduction of TGF-β and related factors, intracellular signaling proteins were identified as Smad. In previous study, it has been reported that Smad 1 and Smad 5, which belong to the R-Smad family mediate BMP signaling, were involved in the induction of osteoblast differentiation in C2C12 cells. To understnad the role of Smads involved in osteogenic transdifferentiation in C2C12 cell, in present study, after we stably transfected C2C12 cells with each. Smad(Smad 1, Smad 5) expression vector, cultured for 3 days and stained for alkaline phophatase activity. ALP activity positive cells appeared in the Smad 1, Smad 5 stably transfected cell even in the abscence of BMP. After transiently co-transfected C2C12 cells with each Smad expression vector and ALP promoter, it was excence of MBP. These result suggested that both Smad 1 and Smad 5 were involved in the intracellular BMP signals which induce osteoblast differentiation in C2C12 cells. The effect of BMP on C2C12 cells with Smad 1, Smad 5 transfected were studied by using northern blot analysis. the treatment of BMP upregulated ALP mRNA level in three groups, especially upregulation of ALP was larger in Smad 1, Smad 5 transfected cell than control group. Pretreatment with cycloheximide(10㎍/ml), a protein synthesis inhibitor resulted in blocking the ALP gene expression even in BMP(100ng/ml) treated cell. These results suggested that Smad increased the level of ALP mRNA via the synthesis of a certain transcriptional regulatory protein.

9세 소아에서 나타난 비구개낭의 치험례 : CASE REPORT

이상호,모경희,박종휘,이난영,이창섭,윤정훈 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.3

비구개낭은 절치관낭이라고도 불리우며 가장 흔한 비치성낭이다. 발육성으로 비구개관내에서 태생 상피잔사가 증식하여 낭변성을 일으킴으로써 발생한다. 비구개낭은 악골낭의 약 1.7-11.9%를 차지하며 남자에게서 약 3배 정도 호발한다. 연령분포는 매우 광범위하나 대개 40대에서 60대 사이에서 발견되며 10세 이전에 발견되는 경우는 매우 드물다. 비구개낭을 위한 적절한 치료는 적출이며 비구개신경에 손상을 가하지 않도록 해야 한다. 낭이 커서 치아의 생활력에 영향을 주거나 비구강누공이나 구강 상악동 누공을 형성할 우려가 있을 경우에는 조대술로 치료할 수 있다. 본 증례의 환아는 구개부의 무통성 종창을 주소로 내원하여 임상 및 방사선 검사를 실시한 결과 소아에서 매우 드물게 나타나는 비구개낭으로 방사선사진에 32×25.5mm크기를 나타내었으며, 매우 팽창되어 순측 피질골판을 관통하고 상순소대 하방과 전정부위까지 종창되어 있었다. 병소와 인접한 상악 4전치 치근은 심하게 이개되어 있었다. 낭의 크기가 매우 커서 조대술을 시행하였으며 obturator를 장착하고 있으며 향후 계속 관찰이 요한다. Nasopalatine duct cyst(NPDC) is the most common non-odontogenic cyst in the oral area and is thought to be originated from the epithelial remnants of the nasopalatine duct. Many etiologic factors have been proposed for the NPDC; trauma to the region during mastication of from ill fitting dentures, bacterial infection, and spontanious proliferation of tissue. The majority of cases are seen between the ages of 40th and 60th and it is rare in children. Surgical enucleation is the recommended treatment for NPDC, usually under general anaesthesia. A 9-year-old boy was refered to the chosun university pedodontics clinic that this child who has anterior palatal swelling is in routine check. In this case, swelling was presented at the bottom of the anterior nasal cavity as well as in the labial aspect of the upper alveolar ridge. It was so large that we treated it by marsupialization and obturator was put. This patient is on the continuous observation. We treated the child who had NPDC by marsupialization, and got the successful results such as rapid bone regeneration and the consistency of incisor vitality.

Effect of cadmium toxicity to Paranura rosea at two different temperatures

Nan-Hee Yang,Jino Son,Hyoung-ho Mo,Kijong Cho 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

In Korea very few studies have been conducted in relation to collembola sensitivity to pollutants in populations exposed to soil contaminants in particular heavy metals. The objective of the current research were to study the thermal effects of selected heavy metals on the survival and reproduction of P. rosea in order to assess its applicability as a test species. Petri dishes (87 mm diameter, 15 mm height) were used for compact soil toxicity test and were filled to a depth of 0.5 mm with 6 g artificial soil and 3.5 mL distilled water was added. 20 adults (6 weeks from egg stage) were introduced to each petri dish and placed in two incubators set at 20℃ and 25℃, respectively under continuous darkness. The 28 days EC50s for cadmium in compact soil at 20℃ and 25℃ were 48.9031 and 91.3585 mg/kg, respectively. This thermal toxicity test of P. rosea is advantageous for various environmental sudies, such as toxicity tests.

宿主의 免疫狀態가 Ethyl urethane 의 發癌性에 미치는 影響

文鸞模(Nan Mo Moon) 대한외과학회 1978 Annals of Surgical Treatment and Research Vol.20 No.6

이하선 종양에 대한 임상적 고찰

문난모(Nan Mo Moon),김진복(Jin Pok Kim) 대한외과학회 1976 Annals of Surgical Treatment and Research Vol.18 No.11

서혜부 탈장의 임상적 고찰

문난모(Nan Mo Moon),박길수(Kilsoo Park) 대한외과학회 1975 Annals of Surgical Treatment and Research Vol.17 No.9

불소 바니쉬의 우식 예방 효과

이상호 ( Sang Ho Lee ) , 조난주 ( Nan Ju Cho ) , 윤창륙 ( Chang Lyuk Yoon ) , 안종모 ( Jong Mo Ahn ) , 이난영 ( Nan Young Lee ) 조선대학교 구강생물학연구소 2007 Oral Biology Research (Oral Biol Res) Vol.31 No.2

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Effects of Long-Term Fertilization on Microbial Diversity in Upland Soils Estimated by Biolog Ecoplate and DGGE

Nan-Hee AN,Sang-Min Lee,Jung-Rai Cho,Byung-Mo Lee,Jae-Hun Shin,Jung-Hun Ok,Seok-Cheol Kim 한국토양비료학회 2014 한국토양비료학회지 Vol.47 No.6

Organic amendment practices can influence diversity and activities of soil microorganisms. There is a need to investigate this impact compared with other types of materials. This study was carried out to evaluate the long term effects of chemical and organic fertilizer on soil microbial community in upland field. During the last 11 years green manure, rice straw compost, rapeseed cake, pig mature compost, NPK, and NPK + pig mature compost were treated in upland soil. Organic fertilizer treatment found with high bacterial colony forming units (CFUs) as compared to chemical and without fertilizer treatment. There was no significant difference in the actinomycetes and fungal population. The average well color development (AWCD) value was the highest in green manure and, the lowest in without fertilizer treatment. Analyses based on the denaturing gradient gel electrophoresis (DGGE) profile showed that rice straw compost and pig mature compost had a similar banding pattern while rapeseed cake, NPK, NPK + pig mature compost and without fertilizer treatment were clustered in another cluster and clearly distinguished from green manure treatment. Bacterial diversity can be highly increased by the application of organic fertilizer while chemical fertilizer had less impact. It can be concluded that green manure had a beneficial impact on soil microbial flora, while, the use of chemical fertilizer could affect the soil bacterial communities adversely.

Physicochemical Properties of Organic Liquid Fertilizer with Oil Cake and Rice Bran as Affected by Microorganism and the Ratio of Molasses

Nan-Hee An,Yong-Ki Kim,Jung-Rai Cho,Hyeong-Jin Jee,Byung-Mo Lee,Jong-Chul Yoon,Ji-Won Choi 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6

The study was conducted to investigate changes in the characteristics of inorganic components during fermenting process of organic liquid fertilizers according to the type and ratio of microorganism and the amount of molasses when producing organic liquid fertilizers using sesame oil cake and rice bran. To select appropriate microorganisms, liquid fertilizers were produced through a 90-day fermentation process by adding mag-ggeo-li, yogurt, dried yeast, and leaf mold. The pH in liquid fertilizer was decreased, and then increased in all microorganism samples except the mag-ggeo-li sample. The EC was rapidly increased in all samples until the 10th days after production, and showed no changes after the 60<SUP>th</SUP> days in dried yeast and after the 30<SUP>th</SUP> days in the other samples. The concentration of NH₄-N was generally increased with time. The concentration of P₂O? was rapidly increased until the 10<SUP>th</SUP> days after production and was maintained at about 1% regardless of the type of microorganism. In terms of the characteristics of liquid fertilizers according to the ratio of selected dried yeast, the pH was decreased until the 30<SUP>th </SUP>days after producing the liquid fertilizers, and then was increased regardless of the ratio of dried yeast. The EC was increased with time and showed no differences depending on the amount of dried yeast. The concentration of NH₄-N was increased with time and in proportion to the amount of dried yeast. In terms of the characteristics of liquid fertilizers according to the ratio of molasses, the pH was decreased with increasing the molasses. The EC and concentration of P₂O? were no differences according to the amount of molasses. When 3% molasses was added, the content of NH₄-N was 2.6 mg L<SUP>-1</SUP> at the beginning and was at 3,025 mg L<SUP>-1</SUP> on the 90th days.