조혈모세포이식환자에서 조혈기능의 회복과 관련된 인자들에 관한 연구

정기주,강명수,권기두,김경하,이상철,김현정,배상병,김찬규,이남수,이규택,원종호,홍대식,박희숙 순천향대학교 의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.1

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발달성 고관절 이형성증 환자에 발생된 고도의 탈구에 대한 전자하부 단축 절골술을 통한 인공고관절 치환

박명식,서동주,박종혁,오상수,황병연 대한고관절학회 2004 Hip and Pelvis Vol.16 No.1

사업장 소음 폭로에 의한 일과성 역치 상승과 회복

조수헌,하미나,한상환,주영수,성주헌,강종원,윤덕로,송동빈,이명학,김선태 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.2

To determine the recovery time from noise-induced temporary threshold shift(TTS), a prospective field study was conducted at three worksites where workers are known to be exposed high level of noise. Subjects were selected according to answers on a questionnaire which inquired about otological history and previous noise exposure, including avocational, military and occupational exposures. After excluding employee with past otologic problems, recent exposure to high level noise, and under medications, total 92 employees participated in the study. Among 92 participants, complete consecutive audiometric examinations were carried out at 0∼2 hours, 5∼7 hours, 14∼16 hours after worktime noise exposure on 26 participants wearing hearing protectors and 22 participants wearing no protective devices. The difference between the hearing level 0∼2 hours after noise exposure and 5∼7 hours is statistically significant by paired t-test(p<0.01). The median recovery times calculated from the data of 22 participants wearing no protective hearing devices are 15.6 hours at 4000Hz, and 7.7 hours, 10.3 hours, 8.4 hours at 1000Hz, 2000Hz and 8000Hz respectively. These data suggest that when measuring the pure tone audiometry for noise exposed workers, at least 16 hours noise-free interval is required.

흰쥐의 브르모벤젠대사계에 미치는 어성초의 영향과 페놀성 화합물의 분리

박종철(Jong Cheol Park),허종문(Jong Moon Hur),박주권(Ju Gwon Park),박성종(Sung Jong Park),이종호(Jong Ho Lee),성낙주(Nak Ju Sung),최명락(Myung Rak Choi),송상호(Sang Ho Song),김문성(Moon Sung Kim),최종원(Jong Won Choi) 한국생약학회 2000 생약학회지 Vol.31 No.2

Effects of Houttuynia cordata on the level of lipid peroxide and the enzyme activities of the liver were investigated in bromobenzene-induced rats. Lipid peroxide content in liver was increased by bromobenzene. It was decreased when the methanol extract from the aerial parts of H. cordata was treated to the rat. The methanol extract reduced the activities of aminopyrine N-demethylase and aniline hydroxylase that increased by bromobenzene, however did not affect glutathione S-transferase activity. The methanol extract recovered the activity of epoxide hydrolase activity that decreased significantly by bromobenzene. We suggest that under our experimental conditions the extract might play an important play in the prevention of hepatotoxicity by reduction of aminopyrine N-demethylase and aniline hydroxylase activities as well as enhancement of epoxide hydrolase activity. Six phenolic compounds have been isolated from H. cordata and identified by means of spectral analysis as protocatechuic acid, quercetin, apigenin, afzelin, hyperoside and quercitrin.

Analysis of Immersion Effects According to the Level of Map in Adventure Game

Myung-Ju Kang(강명주),Chan-Il Park(박찬일),Jong-Won Lee(이종원),Hyoun-Ju Oh(오현주),Sang-Jung Kim(김상중) 한국컴퓨터정보학회 2015 韓國컴퓨터情報學會論文誌 Vol.20 No.10

In this paper, we analyze the immersion effects according to the level of map in adventure game. Computer games are actively enjoyed by many people around the world. Game players have frequently experienced the immersion which is an important aspect to be attained from games. However, it is not clear what immersion is and what kinds of factors influence to immersion. Nonetheless, in recent many researches, the elements of humanities and fun were found as factors of immersion in various digital contents such as game, virtual reality, cinema and etc. Therefore, today"s games are developed on the basis of the humanities and fun theory that many researchers have been studied for a long time. In games, the balance of the level of map is related with immersion. Hence, it is important to know how the map patterns of game affect immersion of players. In this paper, we developed a 3D survival adventure game named by "1 Hour" using Unity3D in order to simulate how map patterns affect immersion of players. We also analyzed the relationship between the level of map and immersion using the game.

Isolation and Biological Activity of Sesquiterpene Lactone

Myung Ju Kim,Hyun Ju Oh,Jong Gab Chung,Seung Hwa Baek 한의병리학회 2005 대한동의병리학회 학술대회논문집 Vol.2005 No.8

Edge-carboxylated graphene nanoplatelets as oxygen-rich metal-free cathodes for organic dye-sensitized solar cells

Ju, Myung Jong,Jeon, In-Yup,Lim, Kimin,Kim, Jae Cheon,Choi, Hyun-Jung,Choi, In Taek,Eom, Yu Kyung,Kwon, Young Jin,Ko, Jaejung,Lee, Jae-Joon,Baek, Jong-Beom,Kim, Hwan Kyu The Royal Society of Chemistry 2014 ENERGY AND ENVIRONMENTAL SCIENCE Vol.7 No.3

<P>Edge-carboxylated graphene nanoplatelets (ECGnPs) were synthesized by the simple, efficient and eco-friendly ball-milling of graphite in the presence of dry ice and used as oxygen-rich metal-free counter electrodes (CEs) in organic dye-sensitized solar cells (DSSCs), for the first time. The resultant ECGnPs are soluble in many polar solvents including 2-propanol due to the polar nature of numerous carboxylic acids at edges, allowing an electrostatic spray (e-spray) to be deposited on fluorine-doped SnO<SUB>2</SUB> (FTO)/glass substrates. The ECGnP-CE exhibited profound improvements in the electrochemical stability for the Co(bpy)<SUB>3</SUB><SUP>2+/3+</SUP> redox couple compared to the platinum (Pt)-CE. The charge transfer resistance (<I>R</I><SUB>CT</SUB>), related to the interface between an electrolyte and a CE, was significantly reduced to 0.87 Ω cm<SUP>2</SUP>, much lower than those of (Pt)-CE (2.19 Ω cm<SUP>2</SUP>), PEDOT:PSS-CE (2.63 Ω cm<SUP>2</SUP>) and reduced graphene oxide (rGO)-CE (1.21 Ω cm<SUP>2</SUP>). The DSSC based on the JK-303-sensitizer and ECGnP-CE displayed a higher photovoltaic performance (FF, <I>J</I><SUB>sc</SUB>, and <I>η</I>, 74.4%, 14.07 mA cm<SUP>−2</SUP> and 9.31%) than those with the Pt-CE (71.6%, 13.69 mA cm<SUP>−2</SUP> and 8.67%), PEDOT:PSS (68.7%, 13.68 mA cm<SUP>−2</SUP> and 8.25%) and rGO-CE (72.9%, 13.88 mA cm<SUP>−2</SUP> and 8.94%).</P> <P>Graphic Abstract</P><P>Edge-carboxylated graphene nanoplatelets (ECGnPs) prepared by the dry ball-milling of graphite showed lower charge-transfer resistance (<I>R</I><SUB>CT</SUB>) and better electrochemical stability for the JK-303-based solar cell with Co(bpy)<SUB>3</SUB><SUP>2+/3+</SUP> redox couple. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3ee43732a'> </P>

N-Doped Graphene Nanoplatelets as Superior Metal-Free Counter Electrodes for Organic Dye-Sensitized Solar Cells

Ju, Myung Jong,Kim, Jae Cheon,Choi, Hyun-Jung,Choi, In Taek,Kim, Sang Gyun,Lim, Kimin,Ko, Jaejung,Lee, Jae-Joon,Jeon, In-Yup,Baek, Jong-Beom,Kim, Hwan Kyu American Chemical Society 2013 ACS NANO Vol.7 No.6

<P>Highly efficient counter electrodes (CEs) for dye-sensitized solar cells (DSSCs) were developed using thin films of scalable and high-quality, nitrogen-doped graphene nanoplatelets (NGnP), which was synthesized by a simple two-step reaction sequence. The resultant NGnP was deposited on fluorine-doped SnO<SUB>2</SUB> (FTO)/glass substrates by using electrospray (e-spray) coating, and their electrocatalytic activities were systematically evaluated for Co(bpy)<SUB>3</SUB><SUP>3+/2+</SUP> redox couple in DSSCs with an organic sensitizer. The e-sprayed NGnP thin films exhibited outstanding performances as CEs for DSSCs. The optimized NGnP electrode showed better electrochemical stability under prolonged cycling potential, and its <I>R</I><SUB>ct</SUB> at the interface of the CE/electrolyte decreased down to 1.73 Ω cm<SUP>2</SUP>, a value much lower than that of the Pt electrode (3.15 Ω cm<SUP>2</SUP>). The DSSC with the optimized NGnP–CE had a higher fill factor (FF, 74.2%) and a cell efficiency (9.05%), whereas those of the DSSC using Pt–CE were only 70.6% and 8.43%, respectively. To the best of our knowledge, the extraordinarily better current–voltage characteristics of the DSSC–NGnP outperforming the DSSC–Pt for the Co(bpy)<SUB>3</SUB><SUP>3+/2+</SUP> redox couple (in paticular, FF and short circuit current, <I>J</I><SUB>sc</SUB>) is highlighted for the first time.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2013/ancac3.2013.7.issue-6/nn4009774/production/images/medium/nn-2013-009774_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn4009774'>ACS Electronic Supporting Info</A></P>

Peroxisome proliferator-activated receptor γ 배위자의 간암 세포에 대한 항암 효과

채명종 ( Myung Jong Chae ),심재준 ( Jae Jun Shim ),김병호 ( Byung Ho Kim ),황보영 ( Bo Young Hwang ),이영주 ( Young Ju Lee ),하승형 ( Seung Hyung Ha ),장재영 ( Jae Young Jang ),동석호 ( Seok Ho Dong ),김효종 ( Hyo Jong Kim ),장영 대한내과학회 2008 대한내과학회지 Vol.75 No.3

목적: PPARγ는 핵 수용체의 일종으로 혈당 조절과 지방세포의 분화에 작용하는 것뿐만 아니라 각종 암세포에 대한 항암 작용과도 연관되어 있다고 알려져 있다. 대표적인 합성 PPARγ 배위자인 thiazolidinediones계 혈당 강하제와 내인성 배위자인 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2)가 간세포암(이하 간암)에도 항암 효과가 있는지 조사하였다. 방법: 간암 세포주로는 HepG2, Hep3B, PLC/PRF5를 사용하였는데, 이들 세포주에서 PPARγ 유전자 발현은 역전사-중합효소연쇄반응으로 확인하였다. Thiazolidinediones계 약물인 troglitazone (TGZ), pioglitazone (PGZ) 그리고 rosiglitazone (RGZ)을 각각 25~100 μM의 농도로 각 세포주에 투여하였고, 15d-PGJ2는 20~80 μM의 농도로 투여하였다. MTT assay를 이용하여 세포 생존률을 72시간까지 대조군과 비교하였다. 세포 주기 분석은 flow cytometry를 이용하였고, 세포자멸사는 ELISA kit를 이용하였다. Caspase-3와 caspase-8 그리고 caspase-9의 활성도를 caspase colorimetric assay를 이용하여 분석하였다. Caspase 억제제를 투여하여 PPARγ 배위자에 의한 세포자멸사가 억제되는지 조사하였다. 결과: 사용된 PPARγ 배위자 중 TGZ과 15d-PGJ2가 HepG2, Hep3B 그리고 PLC/PRF/5에 뚜렷한 성장 억제를 나타냈다. 반면에 PGZ과 RGZ은 TGZ에 비해 거의 효과가 없었다. 세포 주기분석에서도 TGZ과 15d-PGJ2가 G0/G1 arrest와 세포자멸사를 일으킨 반면 PGZ과 RGZ은 효과가 없었다. 세포 주기 및 세포자멸사 분석에서 TGZ과 15d-PGJ2의 항암효과는 세포주에 따라 서로 다르게 나타났다. TGZ은 HepG2에서 G0/G1 arrest를 일으켰고 PLC/PRF5의 세포자멸사를 유도한 반면 15d-PGJ2는 HepG2에서 세포자멸사를 일으키고 PLC/PRF5에서는 뚜렷한 세포자멸사를 일으키지 못하였다. TGZ과 15d-PGJ2은 caspase-3 활성도를 크게 증가시켰으며 (p<0.01) caspase-9의 활성도도 증가시켰다. TGZ과 15d-PGJ2에 의해 유도된 세포자멸사는 pancaspase 억제제와 caspase-3 특이 억제제에 용량 의존적으로 억제되었다. 결론: Troglitazone과 15d-PGJ2는 간암 세포에서 세포자멸사를 유도하여 항암 작용을 나타내는데, PPARγ 이외의 다른 기전이 작용하는 것으로 보이며 항암 기전도 서로 다른 것으로 생각한다. Background/Aims: Thiazolidinediones, which are synthetic insulin sensitizers, are known activators of peroxisome proliferator-activated receptor gamma (PPARγ). PPARγ ligands, including endogenous 15-deoxy-Δ12,14-prostaglandin J2(15d-PGJ2), are thought to elicit antineoplastic effects in various cancer cells. In this study, the antineoplastic effects of PPARγ ligands against hepatocellular carcinoma (HCC) cells were investigated. Methods: HepG2, Hep3B, and PLC/PRF5 cells were cultured with troglitazone (TGZ), pioglitazone (PGZ), rosiglitazone (RGZ), or 15d-PGJ2 at concentrations of 20-100 μM.. Cell viability, cell cycle arrest, apoptosis, and caspase activity were measured using the MTT assay, flow cytometry, enzyme-linked immunosorbent assay (ELISA), and colorimetric assays, respectively. The effects of various caspase inhibitors were also measured using a cell death detection ELISA. Results: All three cell lines expressed the PPARγ gene. TGZ and 15d-PGJ2 strongly inhibited growth in HepG2, Hep3B, and PLC/PRF5 cells. In contrast, PGZ and RGZ showed a much weaker effect in all cell lines. In terms of cell cycle arrest and apoptosis, TGZ induced G0/G1 arrest in HepG2 cells and increased the apoptotic fraction in Hep3B and PLC/PRF5 cells. In contrast, 15d-PGJ2 induced apoptosis only in HepG2 and Hep3B cells. TGZ and 15d-PGJ2 increased caspase-3 activity significantly and increased caspase-9 activity slightly. TGZ- and 15d-PGJ2-induced apoptoses were inhibited by a pancaspase inhibitor (Z-VAD-FMK) and a caspase-3 specific inhibitor (Z-DEVD-FMK) in a dose-dependent manner. Conclusions: TGZ and 15d-PGJ2 elicit antineoplastic effects in various HCC cells via caspase-dependent apoptotic induction. Their differential effects on similar cell types suggest that another antineoplastic mechanism, most likely a PPARγ-independent pathway, is involved. (Korean J Med 75:288-299, 2008)

원발성 비후형 심근증에서 심근비후 형태에 따른 임상상의 차이

이명곤(Myung Kon Lee),박종수(Jong Su Park),안영근(Young Keun An),박주형(Ju Hyung Park),정명호(Myung Ho Jeong),조정관(Jeong Gwan Cho),박종춘(Jong Chun Park),강정채(Jung Chaee Kang),박옥규(Ock Kyu Park) 대한내과학회 1993 대한내과학회지 Vol.45 No.4

N/A Background; The idipathic hypertrophic cardiomyopathy(HCM) is characterized by inappropriate myocardial hypertrophy of unknown cause. It may presents a variety of clinical and morphologic features according to the site and extent of the hypertrophy. The widespread application of echocardiography has made it possible to diagnose HCM earlier and easier, even in asymptomatic patients. However, clinical reports on the relationship between the distribution of left ventricular hypertrophy (LVH) and the clinical features are few in Korea. Therefore, the present clinical stu4y was performed to characterize more completely the distribution of LVH and to determine whether different patterns of hypertrophy are of particular clinical significance. Methods: The clinical, electrocardiographic and echocardiographic features were reviewed in 32 patients with idipathic HCM of Chonnam National University Hospital from July 1983 to August 1992. Results: 1) There were 23 males and 9 females. The mean age was 45.4±16.5 ranging from 15 to 74 years. There were no significant difference in age distribution according to the patterns of LUH. 2) Based on M-mode and 2-dimensional echocardiographic findings. The 32 patients were divided into 2 groups; 16 patients in the obstructive HCM group and the other 16 patients in the non-obstructive HCM group. Among the 16 patients with non-obstructive HCM, there were 6 patients with asymmetric septal hypertrophy(septal HCM), 7 patients with apical hypertrophy (apical HCM), 3 patients with symmetric (concentric) hypertrophy (symmetric or concentric HCM). 3) The cardinal clinical symptoms were dyspnea (71.9%), chest pain (59.5%), palpitation(37.5%) and syncope (12.5%). There were no significant difference in the frequency of symptoms according to the patterns of LCH. 4) On chest X-ray examination, the enlargement of cardiac silhoutte (C/T ratiok≥0.55) was observed in 32% of the cases. There wedre 4 patients with C/T ratio ≥0.6, and all had obstructive HCM. 5) In conventional 12 leads electrocardiograms, abnormal electrocardiographic findings were obtained in 94% of the cases. Among them repolarization abnormalities and left ventricular hypertrophy were most common, occuring in 80.6% and 64.5%, respectively. This was followed by abnormal Q waves (37.5%), atrial fibrillation (29.0%) and giant T wave inversion (29.0%). Giant T wave inversion was significantly more common in patients with apical HCM than in any other patients. 6) On the M-mode echocardiograms, the ratio of left ventricular septal thickness to left ventricular posterior wall thickness (IVS/LVPW) was 1.58±0.36 in obstructive HCM, 1.55±0.08 in septal HCM, 1.05±0.14 in apical HCM, and 1.13±0.08 in symmetric HCM. 7) On the 2-dimensional echocardiograms, the ventricular septum and anterolateral free wall were significantly thicker compared to left ventricular posterior wall in patients with obstructive and septal HCM (p<0.001, p<0.01 respectively). The apical wall was significantly thicker compared to the other regions in patients with apical HCM. 8) Mital regurgitation was detected by Doppler echocardiography in 13 (40.6%) of the total 32 cases of HCM and especially in 11 (68.8%) of the 16 cases with obstructive HCM. 9) Continuous wave Doppler echocardiography of left ventricular outflow tract was performed in 10 patients with obstructive HCM and revealed a mean peak pressure gradient of 50.1±39.9mmHg (13~130mmHg) between the left ventricular (LV) mid cavity and the LV outflow tract. Conclusion: Clinical features are very similar in every pattterns of LVH. But the above results suggested that depending on the extent and distribution of LVH, the functional and morphologic features of HCM may differ considerably. It is still questionable whether identifiable patterns of hypertrophy are of clinical significance.