서울시내 고소득층 아파트단지 국민학교 어린이의 체격과 식생태에 관한 조사연구

모수미,손숙미,김복희,김주혜,김희경,최혜미 한국식생활문화학회 1993 韓國食生活文化學會誌 Vol.8 No.3

An ecological survey of food and nutrition was conducted in April and May, among 278 children, 6 to 12 years old, of the Youido elementary school, which offers no school lunch program, and is situated in Youido delta of Han River side of Seoul, known as the location of one of the socioeconomically high groups; Eighteen percent and 18.3% of subjects were proven to be overweight and obese, respectively. With regard to frequency of skipping breakfast, 28.4% of the subjects skipped breakfast often and 2.5% of subjects skipped breakfast every morning. Obese children showed good appetite and the time consumed for eating was shorter than those of normal or overweight children. Most serious anxiety among the mothers for those children was food habit of 'eating rapidly' and 'overeating'. Another striking observation was that 14.4% of the subjects ate only one side-dish with boiled rice, and such a pattern was more often found in the normal or slim group. Most of the subjects had their snacks after being back in home from school. Thirty-one percent of the children had snacks when they felt hungry, 69% had snacks for various reasons such as 'habitually', 'for unwinding', and 'mother gives me snacks'. Thirty-eight percent of the boys and 19% of the girls had physical exercise everyday and overweight and obese children got exercise more frequently. But the time consumed for exercise was much more shorter than that of normal subjects. Most of the children liked fruits, meats, and biscuit, in contrast, lowest preferency was for vegetables, fermented fish products, and boiled rice mixed with other grains. Obese children experienced more kinds of food than normal or slim group. The study found that habits of overeating due to good appetite and eating rapidly were recognized as one of dominant reasons causing obesity among children. On the other hand, serious dietary problem of children of normal or slim group was eating less variety of food. Active nutrition education for both children and mothers will be recommended with joint participation of teachers.

Camptothecin activates SIRT1 to promote lipid catabolism through AMPK/FoxO1/ATGL pathway in C2C12 myogenic cells

Mei-Chen Lo,Jia-Yin Chen,Yung-Ting Kuo,Wei-Lu Chen,Horng-Mo Lee,Shyang-Guang Wang 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.8

Caloric restriction activates sirtuin 1 (SIRT1)and induces a variety of metabolic effects that are beneficialfor preventing age-related disease. The present studyscreened a commercially available used drug library todevelop small molecule activators of SIRT1 as therapeuticsfor treatment of metabolic disorders. Using an in vitrofluorescence assay, the cancer therapeutic camptothecinincreased SIRT1 enzymatic activity by 5.5-fold, indicatingit to be a potent SIRT1 activator. Camptothecin also elevatedthe nicotinamide adenine dinucleotide (NAD)?/NADH ratio and increased SIRT1 protein levels in differentiatedC2C12 myogenic cells. Treatment of C2C12 myotubeswith camptothecin increased phosphorylation ofAMP-dependent kinase (AMPK) and acetyl-coenzyme Acarboxylase, caused nuclear translocation and deacetylationof forkhead box O1 (FoxO1), increased transcriptionand protein expression of adipose triglyceride lipase(ATGL), decreased the amount of intracellular oil droplets,and significantly increased b-oxidation of fatty acids. These in vitro data were confirmed in vivo as camptothecintreatment of C57BL/6J mice reduced fat and plasmatriglyceride levels. All of the above camptothecin-inducedalterations were attenuated by the SIRT1-specific inhibitornicotinamide and/or 6-[4-(2-piperidin-1-ylethoxy) phenyl]-3-pyridin-4-ylpyrazolo [1,5-a]pyrimidin (compound C). Thus, camptothecin activation of SIRT1 promotes lipidcatabolism through AMPK/FoxO1/ATGL signaling.

Identification of Polymorphisms in Thymic Stromal Lymphopoietin (TSLP) Gene and Their Association with Allergic Rhinitis

Mei Hua Zhang,Yong Shin Kim,Eun Heui Jin,Ki Mo Kim,Jae Hoon Lee,Chun Shi Li,Qing Gao Zhang,Ki Jung Yun,Soo Cheon Chae,Hun Taeg Chung 한국유전학회 2008 Genes & Genomics Vol.30 No.5

Dendritic cell (DC) activator, thymic stromal lymphopoietin (TSLP) induces DCs to produce Th2-attracting chemokines, causing differentiation of CD4+ and CD8+ T cells into effector cells. The differentiated cells are characterized by a typical pro-allergic phenotype. In this study, we identified four single nucleotide polymorphisms (SNPs) and one variation site (g.-305delT) from the results of scanned human TSLP gene. The SNPs are as following: g.-1914A>G and g.-847C>T in promoter, g.-82C>T in 5` UTR and g.1117C>T in intron 2. We also evaluated the association of genotype and allele frequencies of these SNPs between non-allergic rhinitis controls and allergic rhinitis patients. We further investigated possible correlations between each genotype with serum total IgE levels and peripheral blood eosinophil counts in allergic rhinitis patients. The frequencies of haplotype constructed by these SNPs between these two groups were also compared. Our results demonstrate the two SNPs (g.-1914A>G and g.-847C>T) were associated with susceptibility of allergic rhinitis (P=0.006 and P=0.015 respectively), however, serum total IgE levels were not significantly related with the SNPs.

Two new quinones from the roots of Juglans mandshurica

Mei Jin,Jinfeng Sun,Ren Li,Shengbao Diao,Changhao Zhang,Jiong-Mo Cui,손종근,Wei Zhou,Gao Li 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.9

Two new quinones, 1-hydroxy-5-pentyl-anthraquinone (1) and 4-(5-hydroxy-1,4-dioxo-1,4-dihydronaphthalen- 2-ylamino)-butyric acid methyl ester (2), together with two known quinones, 5-hydroxy-2-(2-hydroxy- ethylamino)-(1,4) naphthoquinone (3) and juglone (4) were isolated from the roots of Juglans mandshurica (Juglandaceae). Their structures were elucidated on the basis of spectral data. Compound 3 was isolated from the Juglans genus for the first time. Compounds 1–4 exhibited significant cytotoxicity towards cultured MDA-MB231, HepG2 and SNU638 cells with IC50 values ranging from 4.46 to 88.47 μM.

A Project to Improve the Pressure Sore Due to Elastic Bandage after Endovascular Vein Harvest Surgery

Mo-Ying Liao,Mei-Fang Chen,Wan-Ching Chao 한국간호과학회 2007 한국간호과학회 학술대회 Vol.2007 No.11

Challenges and Advances in Materials and Fabrication Technologies of Small-Diameter Vascular Grafts

Mei‑Xian Li,Qian‑Qi Wei,Hui‑Lin Mo,Yu Ren,Wei Zhang,Huan‑Jun Lu,정윤기 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

The arterial occlusive disease is one of the leading causes of cardiovascular diseases, often requiring revascularization. Lack of suitable small-diameter vascular grafts (SDVGs), infection, thrombosis, and intimal hyperplasia associated with synthetic vascular grafts lead to a low success rate of SDVGs (< 6 mm) transplantation in the clinical treatment of cardiovascular diseases. The development of fabrication technology along with vascular tissue engineering and regenerative medicine technology allows biological tissue-engineered vascular grafts to become living grafts, which can integrate, remodel, and repair the host vessels as well as respond to the surrounding mechanical and biochemical stimuli. Hence, they potentially alleviate the shortage of existing vascular grafts. This paper evaluates the current advanced fabrication technologies for SDVGs, including electrospinning, molding, 3D printing, decellularization, and so on. Various characteristics of synthetic polymers and surface modification methods are also introduced. In addition, it also provides interdisciplinary insights into the future of small-diameter prostheses and discusses vital factors and perspectives for developing such prostheses in clinical applications. We propose that the performance of SDVGs can be improved by integrating various technologies in the near future.

저자서지결합분석을 통한 중국 IT아웃소싱 연구 동향 분석

려매(Mei Lu),안준모(Joon Mo An),민형진(Hyoung Jin Min) 건국대학교 경제경영연구소 2015 商經硏究 Vol.40 No.2

최근 IT아웃소싱에 관한 연구는 많은 학자들에 의해 다양한 주제와 관점에서 진행되어 왔다. 특히 세계 경제에서 중국의 역할이 증대되고 있는 상황에서 보다 체계적인 연구를 위한 필요성을 인식하고 연구자들에게 기초연구자료를 제공하기 위한 목적으로 중국의 IT 아웃소싱 분야 연구 동향을 분석하고자 한다. 이러한 목적으로 중국의 IT 아웃소싱 연구 동향을 파악하기 위하여 최신 연구주제와 현재 활발히 연구를 수행하는 연구자와 최근 연구 주제 파악이 가능한 저자서지결합분석 기법으로 분석을 수행하였다. 이 연구를 위하여 1998년부터 2013년까지 중국 전자저널데이터베이스 CAJ(China Academic Journal)의 핵심저널에 등재된 IT 아웃소싱 연구를 분석대상으로 하여 논문 게재 빈도가 높은 순으로 상위저자를 33명을 선정하고 피어슨 상관계수행렬을 도출하여 군집분석과 다차원척도법을 적용하여 각 저자들을 군집화 하고 이를 다차원척도 지도에 가시화 하여 중국 IT 아웃소싱 분야 연구 트렌드를 확인하였다. 분석 결과 중국 IT 아웃소싱 세부 주제 분야는 지식이전, IT 아웃소싱 역량평가모델, 서비스 공급자평가, IT 아웃소싱 계약관리, IT 아웃소싱 경쟁력평가, IT 아웃소싱 관계/영향/성과, IT 아웃소싱 위험관리로 나타났다. 군집분석에 의한 저자군집과 IT 아웃소싱의 세부주제 분야를 MDS 지도상에 표현하여 중국의 주요 연구자들과 연구 동향을 시각적으로 확인하였다. 이 연구는 처음으로 순수 중국의 연구문헌 데이터로 분석을 진행하여 중국 IT 아웃소싱 분야의 활발히 진행되고 있는 연구 주제 분야와 주요 연구저자를 확인하였다는 점에서 의의가 있다. Recently, research on IT outsourcing has become the focus of attention in both the industry and academia, many scholars have studied various themes and perspectives, the quantity of research literature is also increasing. To date, IT Outsourcing research in China, this was no attempts have been made to examine the intellectual structure of these researches. Therefore, to master the Intellectual structure of the Chinese IT Outsourcing research, this study used Author Bibliographic Coupling Analysis. This analysis method can analyze the latest research topics and researchers, also can analyze the young researchers. For this study, the theses published on CAJ(China Academic Journal) were reviewed among the researches regarding IT Outsourcing studies from 1998 to 2013 and top 33 authors were selected. Derived the Pearson’s correlation coefficient matrix, then carried out clustering analysis and multi-dimension scaling to visually express the intellectual structure of Chinese IT Outsourcing research area and proposed sub sectors of research subjects. The significance of this study, this is the first study to use pure China literature data analysis confirmed the recently research subjects and research authors of Chinese IT Outsourcing field.

Scutellaria Extract Decreases the Proportion of Side Population Cells in a Myeloma Cell Line by Down-regulating the Expression of ABCG2 Protein

Lin, Mei-Gui,Liu, Li-Ping,Li, Chen-Yin,Zhang, Meng,Chen, Yuling,Qin, Jian,Gu, Yue-Yu,Li, Zhi,Wu, Xin-Lin,Mo, Sui-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background and Aims: Scutellaria is one of the most popular traditional Chinese herbal remedies against various human diseases, including cancer. In this study, we examined the active effects of Scutellaria extract and its main flavonoid constituents on the proportion of side population cells within human multiple myeloma cell line RPMI8226 in vitro and explored the potential molecular mechanisms involved. Materials and Methods: The contents of flavonoids in ethanolic extract of Scutellaria baicalensis Georgi were determined using high performance liquid chromatography. The antiproliferative effect of the ethanolic extract on RPMI-8226 was determined by CCK assay. Apoptosis was measured by annexin combining with propidium iodide in a flow cytometer. Cell cycle analysis was performed by propidium iodide staining in combination with flow cytometry analysis. Hoechst 33342 exclusion assay was used for the identification of side population within RPMI8226 cells. The expression of ABCG2 protein was assessed by Western blotting assay. Results: The content of major flavonoids constitutents of Scutellaria extract was baicalin (10.2%), wogonoside (2.50%), baicalein (2.29%), and wogonin (0.99%), respectively. The crude Scutellaria extract did not show significant anti-proliferative effect, apoptosis induction and cell cycle arrest in RPMI-8226 within the concentrations of $1-75{\mu}g/mL$. However, the ethanolic extract, baicalein, wogonin and baicalin reduced the side population cells in RPMI-8226, and data showed that baicalein and wogonin had stronger inhibitory effects. Correspondingly, they also exhibited significant effects on decreasing the expression level of ABCG2 protein in RPMI-8226 in vitro. Conclusions: Our results for the first time demonstrated a novel mechanism of action for Scutellaria extract and its main active flavonoids, namely targeting SP cells by modulating the expression of ABCG2 protein. This study provides an insight for new therapeutic strategies targeting cancer stem cells of multiple myeloma.

Computational prediction integrating the inhibition kinetics of gallotannin on α-glucosidase

Yue, Li-Mei,Lee, Jinhyuk,Zheng, Li,Park, Yong-Doo,Ye, Zhuo-Ming,Yang, Jun-Mo Elsevier 2017 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.103 No.-

<P><B>Abstract</B></P> <P>Due to the finding that inhibition of α-glucosidase is directly associated with treatment of several diseases, the development of a selective inhibitor for targeting α-glucosidase is important. Gallotannin (GT) is a natural ingredient that has been used as a food additive and for medicinal applications. In this study, we performed a computational docking experiment involving the pre-simulation of the binding mechanism of GT, and the effect of GT on α-glucosidase was evaluated with inhibitory kinetics based on its polyphenol properties. The computational simulations indicated that the hydroxyl groups of GT interact with several residues near the α-glucosidase active site (Met69, Tyr71, Phe177, Arg212, Asp214, Glu276, His348, Asp349, and Arg439), which could affect the catalytic function of α-glucosidase by retarding substrate access. Subsequent kinetic experiments showed that GT conspicuously inhibited α-glucosidase in a parabolic mixed-type manner (<I>IC</I> <SUB>50</SUB> =1.31±0.03μM; <I>K<SUB>i</SUB> </I> =0.41±0.032μM). Our study provides insight into the inhibition mechanism and binding manner of GT to α-glucosidase. Based on its α-glucosidase-inhibiting effect and its demonstrated safety as a naturally derived compound, GT represents a promising potential agent for treatment of α-glucosidase-associated diseases.</P>

ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses NFAT and p21 pathway

( Yue Qun Wang ),( Jun Mei Zhou ),( Xiang Li Ye ),( Yong Qi Wan ),( Yong Qing Li ),( Xiao Yan Mo ),( Wu Zhou Yuan ),( Yan Yan ),( Na Luo ),( Ze Qun Wang ),( Xiong Wei Fan ),( Yun Deng ),( Xiu Shan Wu 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.3

Zinc finger-containing transcription factors are the largest single family of transcriptional regulators in mammals, which play an essential role in cell differentiation, cell proliferation, apoptosis, and neoplastic transformation. Here we have cloned a novel KRAB-related zinc finger gene, ZNF424, encoding a protein of 555aa. ZNF424 gene consisted of 4 exons and 3 introns, and mapped to chromosome 19p13.3. ZNF424 gene was ubiquitously expressed in human embryo tissues by Northern blot analysis. ZNF424 is conserved across species in evolution. Using a GFP-labeled ZNF424 protein, we demonstrate that ZNF424 localizes mostly in the nucleus. Transcriptional activity assays shows ZNF424 suppresses transcriptional activity of L8G5-luciferase. Overexpression of ZNF424 in HEK- 293 cells inhibited the transcriptional activity of NFAT and p21, which may be silenced by siRNA. The results suggest that ZNF424 protein may act as a transcriptional repressor that suppresses NFAT and p21 pathway to mediate cellular functions. [BMB reports 2010; 43(3): 212-218]