Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

임플란트 hexagon 높이에 따른 임플란트와 주위 조직의 응력분포 평가

박성재,김주현,김소연,윤미정,고석민,허중보 대한치과보철학회 2012 대한치과보철학회지 Vol.50 No.1

PURPOSE. To analyze the stress distribution of the implant and its supporting structures through 3D finite elements analysis for implants with different hexagon heights and to make the assessment of the mechanical stability and the effect of the elements. MATERIALS AND METHODS. Infinite elements modeling with CAD data was designed. The modeling was done as follows; an external connection type ∅4.0 mm×11.5 mm OsstemUSII (Osstem Co., Pusan, Korea) implant system was used, the implant was planted in the mandibular first molar region with appropriate prosthetic restoration, the hexagon (implant fixture’s external connection) height of 0.0, 0.7, 1.2, and 1.5 mm were applied. ABAQUS 6.4 (ABAQUS, Inc., Providence, USA) was used to calculate the stress value. The force distribution via color distribution on each experimental group’s implant fixture and titanium screw was studied based on the equivalent stress (von Mises stress). The maximum stress level of each element (crown, implant screw, implant fixture, cortical bone and cancellous bone) was compared. RESULTS. The hexagonal height of the implant with external connection had an influence on the stress distribution of the fixture, screw and upper prosthesis and the surrounding supporting bone. As the hexagon height increased, the stress was well distributed and there was a decrease in the maximum stress value. If the height of the hexagon reached over 1.2 mm, there was no significant influence on the stress distribution. CONCLUSION. For implants with external connections, a hexagon is vital for stress distribution. As the height of the hexagon increased, the more effective stress distribution was observed. 연구 목적: 본 연구는 hexagon 높이에 따른 임플란트 각 부위와 주위 지지조직의 응력분포를 3차원 유한요소 해석을 통해 평가하여 hexagon 높이가 기계적 안정성에 미치는 영향을 평가하고자 시행되었다. 연구 재료 및 방법: 외측 연결 형태의∅L4.0 mm×11.5 mm USII (Osstem Co., Pusan, Korea) 임플란트 시스템을 이용하여 하악 제 1대구치 부위에 임플란트를 식립하여 보철 수복한 경우를 연구 모델로 가정하고 임플란트 고정체의 외측 연결부인 hexagon의 높이를 각각 0.0 mm, 0.7 mm, 1.2 mm, 1.5 mm로 적용한 CAD data를 유한요소 모형화하였다. ABAQUS 6.4 (ABAQUS Inc., Providence, RI, USA)를 이용하여 산출된 응력 값 중에서 등가응력을 기준으로 각 요소(상부 치관, 지대주 나사, 고정체, 치밀골, 해면골)에서 나타나는 최대 응력 값을 비교 하였다. 결과:외측 연결을 갖는 임플란트의 hexagon의 높이는 고정체, 지대주 나사, 상부 보철물 그리고 주위 지지골에 대해 응력 분산에 영향을 주었다. Hexagon의 높이가 증가할 수록 임플란트의 응력 분산은 더 잘 이루어졌으며, 최대 응력 값의 감소를 보였다. Hexagon의 높이가 1.2 mm 이상이 되면 응력 분포에 더 이상 크게 기여하지 않았다. 결론: 외측연결을 갖는 임플란트에서 hexagon은 응력 분산에 필수적인 요소이며 그 높이가 증가할수록 더욱 효과적인 응력의 분산이 나타났다.

地區單位計劃의 物理的 規制要素가 街路景觀에 미치는 影響에 관한 硏究

정성구,최민후,윤진보,신남수 대한건축학회 2004 대한건축학회논문집 Vol.20 No.10

Emphasis has recently been laid on the importance of street space that constitutes urban space in stimulating and strengthening the functions of the downtown area In most urban areas, however, street space has deteriorated so much that it reveals barrenness This situation is owing not to the lack of proper understanding about the importance of street space planning, but to the paucity of accumulated study on the correlation between the spatial consciousness of man as the subject of a street space evaluation on the one hand and the constituent elements as the object of spatial formation on the other District unit planning is a social system that is concerned with developing street space It maps out ideal urban space for better urban environment It also aims to better utilize urban space in accordance with the size and complexity of a city The purpose of this thesis is to examine the correlation between urban planning control elements regulating constituent elements of street space and the structure of man's psychological evaluation of the streetscape It explores the influence of physical regulatory elements on the streetscape, thus providing basic data and guidelines for planning a better streetscape It makes a psychological analysis of different applications of controlling elements by employing a semantic differential method and a CG simulation The procedure of my research is first to choose adjectival pairs that describe street space through bibliographical study and preliminary survey, and then to select physical regulatory elements concerning district Unit planning My study also shows the influence of the design control elements of distinct unit planning on the streetscape, by experimenting with those elements by means of CG simulation It examines variables on the axis of psychological factors according to the variation of regulatory elements and the degree of the influence of those variables

PI형 퍼지제어기를 이용한 강인한 자기동조 시스템 설계

김민정,이인용,조용성,박윤명,최부귀 동아대학교 정보기술연구소 2001 情報通信硏究所論文誌 Vol.8 No.2

Fuzzy logic controllers(FLC's) have been reported to be successfully used for a number of complex and nonlinear processes. PI-type FLC/s are most common. But kike conventional PI-controllers, performance of PI-type FLC's for higher order systems, systems with integrating elements or large dead time, and also for nonlinear systems may be very poor due to large overshoot and excessive oscillation. The proposed FLC's are also used for marginally stable and unstable systems where well-known Ziegler-Nichols tuned PI or PD controllers exhibit very poor performance. The simulation results showed that in each case the proposed scheme outout forms its conventional counterpart.

제주도 기온 및 강수의 변동과 변화의 관측

김성수,강민협,최흥연 제주대학교 해양과환경연구소 2005 해양과환경연구소 연구논문집 Vol.29 No.2

우울증 환자에 대한 Amineptine Hydrochloride의 임상 효과

강민희,신석철,왕성근,신윤오 최신의학사 1990 最新醫學 Vol.33 No.7

냉동마쇄고추를 첨가한 깍두기의 저장기간에 따른 이화학적 성분 변화 및 관능적 특성

설민숙,황성연,박소희,이현자,김종군 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.5

The purpose of this study was to examine the physico-chemical and sensory characteristics of Kakdugi made with mashed red pepper. With regard to the pH of the Kakdugi. those of the juice from Kakdugi with red pepper powder and of the liquid with mashed red pepper were the highest and lowest immediately after preparation. respectively, but thereafter both slightly decreased, but were similar after the fifth week. Generally. the total acidity of Kakdugi liquid was the higher than that of Kakdtegi juice. With regard to the L value, that of the Kakdugi juice was higher than that of Kakdugi liquid and that of Kakdugi with mashed red pepper washigher than that of Kakdugiuitlr red pepper powder. From the third week, the values of all samples generally decreased. The 'a' value of the Kakdugi liquid with mashed red pepper during fermentation was highest During early fermentation, the juice of Kukdugi with red pepper powder showed a higher value than that of Kakdugi with mashed red pepper, but conversely, from the second week that of Kakdugr with mashed red pepper was higher than that of Kakdugi with red pepper powder. The 'L' value of the juice from Kakdugi with red pepper powder was highest until the second week, but trout the third week that of Kalotugi with trashed red pepper was highest. With respect to the organic acids contents, those of citric, quinic and malic acids decreased, but those of lactic and acetic acids increased during tcrrnentatton progression. In addition, the citric, lacticand malic acids contents of the Kakdugi with mashed red pepper werethe highest, whereas that of quinic acid of the Kakdugi with red pepper powder was the highest. From the forth week, the acetic acid content of the Kakdugi with mashed red pepper was further increased. As a result of the sensory test. Kakdugi with mashed red pepper showed significantly higher values with regard to redness and fresh flavor, hut in overall acceptability in the QDA, appearance and taste in the acceptance test. 'Therefore, our results indicate that mashed red pepper particularly increased the 'a' value and organic acid contents of Kakdugi compared to those of red pepper powder, leading to an increased overall acceptability.

혈당검사 방법 별 지각된 통증정도에 대한 비교 : 시각적 상사 척도 이용

최자윤,장금성,김현오,최옥엽,박민희 성인간호학회 2003 성인간호학회지 Vol.15 No.1

Purpose: The purpose of this study is to determine the level of pain related to a blood sugar test. Specifically, the study attempts to compare the varying degree of pains when different types of blood sugar test are used. Method: A sample of 56 subjects is composed of DM patients admitted to a medical ward of C university hospital in Gwangju. Data were collected from July, 2001 to December, 2001. The blood sugar tests were administered in four different ways: (1) the use of 27G needle only. (2) the use of 27G needle followed by ice-packed treatment, (3) the use of 27G needle after EMLA cream application, and (4) the use of lancet. The degree of pain is measured with a visual analogue scale and performed twice. Result: in both measures, the use of 27G needle only method is shown to cause the highest level of pain in comparison with the rest of methods (F=4.01, p=.01; F=8.14, p=.00). However, the differences in pain between time in all methods were not found to be significant (t=-.85, p=.40; t=.80, p=.42; t=.31, p=.75; t=.19, p=.85). Conclusion: The study results indicate that the method using lanceter is more recommendable than the use of 27G needle only method. Further research is needed to support the current study result with the use of different measurement scales and to determine effective methods of blood sugar test to lower pain and compliance.

수축저감제 혼입률 변화에 따른 콘크리트의 공학적 특성에 관한 연구

한민철,金光華,정규문,정평유,윤성노,한천구 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

In this paper, tests are carried out using concrete incorporating shrinkage reducing agent(SR) in order to investigate the engineering properties of concrete. The contents of SR and W/C are varied. According to test results, as for fresh concrete, as SR content increases, slump and air content shows decline tendency, and setting time is accelerated with increase of SR content and decrease of W/C. As for compressive strength, as SR contents increases compressive strength shows to be decreased. As for the effect of SR on drying shrinkage, it shows to be reduced with increase of SR content. This is due to the reduced surface tensile strength of water and tensile strength of capillary pore in concrete.

충남대학교 기초과학분야의 효율적 육성에 관한 연구

윤화중,진성일,윤민중,윤혜수,이영하,최철규,이천배,신대현 충남대학교 자연과학연구소 1986 忠南科學硏究誌 Vol.13 No.2

Chungnam National University (CNU) is located in Daeduk Science Town, a center of science and technology in Korea, and it is expected to play an important role in the development of science in Korea. With this in mind, the basic natural sciences at CNU should be more actively supported in both education and research. Accordingly analysis and comparison of the basic science program at major college in Korea as well as those in the USA and Europe has been performed. The current cooperative relationship between CNU and research institutes in the science town has also been analyzed. This study has resulted in propositions to make more efficient the upbringing of the basic sciences at CNU, by focusing on the following points ; a) to improve academic affairs, b) to make graduate studies substantial, c) to secure highly qualified faculty members, d) to increase cooperation in research between CNU and reserch institutes, e) to enlarge research laboratories and acquire more equipment and facilities.