The CREG1-FBXO27-LAMP2 axis alleviates diabetic cardiomyopathy by promoting autophagy in cardiomyocytes

Liu Dan,Xing Ruinan,Zhang Quanyu,Tian Xiaoxiang,Qi Yanping,Song Haixu,Liu Yanxia,Yu Haibo,Zhang Xiaolin,Jing Quanmin,Yan Chenghui,Han Yaling 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Autophagy plays an important role in the development of diabetic cardiomyopathy. Cellular repressor of E1A-stimulated genes 1 (CREG1) is an important myocardial protective factor. The aim of this study was to investigate the effects and mechanisms of CREG1 in diabetic cardiomyopathy. Male C57BL/6 J mice, Creg1 transgenic mice and cardiac-specific knockout mice were used to establish a type 2 diabetes model. Small animal ultrasound, Masson’s staining and western blotting were used to evaluate cardiac function, myocardial fibrosis and autophagy. Neonatal mouse cardiomyocytes (NMCMs) were stimulated with palmitate, and the effects of CREG1 on NMCMs autophagy were examined. CREG1 deficiency exacerbated cardiac dysfunction, cardiac hypertrophy and fibrosis in mice with diabetic cardiomyopathy, which was accompanied by exacerbated autophagy dysfunction. CREG1 overexpression improved cardiac function and ameliorated cardiac hypertrophy and fibrosis in diabetic cardiomyopathy by improving autophagy. CREG1 protein expression was decreased in palmitate-induced NMCMs. CREG1 knockdown exacerbated cardiomyocyte hypertrophy and inhibited autophagy. CREG1 overexpression inhibited cardiomyocyte hypertrophy and improved autophagy. LAMP2 overexpression reversed the effect of CREG1 knockdown on palmitate-induced inhibition of cardiomyocyte autophagy. CREG1 inhibited LAMP2 protein degradation by inhibiting the protein expression of F-box protein 27 (FBXO27). Our findings indicate new roles of CREG1 in the development of diabetic cardiomyopathy.

Deep sequencing identifies conserved and novel microRNAs from antlers cartilage of Chinese red deer (Cervus elaphus)

Yanxia Chen,Xuedong Liu,Xiaoguang Yang,Yuhui Liu,Xiaomeng Pi,Qingzhen Liu,Dong Zheng 한국유전학회 2015 Genes & Genomics Vol.37 No.5

Deer antlers are the only mammalian appendages subject to an annual cycle of epimorphic regeneration. Within the rapid-growth stage, they display the fastest cartilage development in the animal kingdom. To identify microRNA (miRNA) profiling in red deer (Cervus elaphus) antler cartilage, we applied deep sequencing technology to a small RNA library constructed from pooled cartilage (three antlers from three individuals). We generated 9,520,645 mappable reads with a size distribution of between 15 and 30 nucleotides (miRNAs of 18 nucleotides were the most abundant group: 31 %). Bioinformatics data mining revealed 399 miRNAs in antler cartilage, of which 345 were highly conserved and expressed in 25 other mammals, including the cattle (Bos taurus) and in humans (Homo sapiens). The remaining 54 miRNAs we identified were novel and likely to be antler-cartilage specific, but were expressed at low levels. The identification of these known and newly identified miRNAs in antler cartilage significantly enhances our understanding of the miRNA profiling of regenerating antler cartilage. Further studies are necessary to better understand miRNAs-regulated antlerogenesis.

Survival of Cancer Patients in Northeast China: Analysis of Sampled Cancers from Population-Based Cancer Registries

Yanxia Li,Liya Yu,Jun Na,Shuang Li,Li Liu,Huijuan Mu,Xuanjuan Bi,Xiaoxia An,Xun Li,Wen Dong,Guowei Pan 대한암학회 2017 Cancer Research and Treatment Vol.49 No.4

Purpose The cancer survival was characterized by following up sampled subgroups of cancer cases from three population-based cancer registries in Northeast China. Materials and Methods Survival analysis was used to analyze 6,871 patients, who had one of the 21 most common cancers based on sampling from the population-based cancer registries of three cities in Liaoning Province. All patients were diagnosed between 2000 and 2002 and were followed up to the end of 2007 by active and passive methods. The 5-year age standardized relative survival rates (ASRS) were estimated for all cancers combined and each of the 21 individual cancers. Results The survival status was traced for 80.8% of 8,506 sampled cancer cases. The 5-year ASRS for all 21 cancers combined was 41.5% (95% confidence interval, 40.3 to 42.7), the highest ASRS was observed for thyroid cancer (85.2%), breast cancer (78.9%), uterine corpus cancer (75.9%), and urinary bladder cancer (70.2%); the lowest 5-year ASRS was noted in pancreatic cancer (8.8%), liver cancer (11.0%), esophageal cancer (18.8), and lung cancer (19.6%). The cancer survival rates in Liaoning cities were similar to those of urban areas in mainland China, but significantly lower than those in Hong Kong, Korea, and Japan. Conclusion The strikingly poor cancer survival rates in three cities of Liaoning Province and in other places in China highlight the need for urgent investment in cancer prevention, early detection, and standardized and centralized treatment.

OBE 理念下计算机网络课程教学改革研究

刘晓青(Xiaoqing Liu),李艳霞(Yanxia Li) YIXIN 출판사 2024 教育教学研究论丛 Vol.2 No.2

Corrigendum to "Ginsenoside Rb1 attenuates methamphetamine (METH)-induced neurotoxicity through the NR2B/ERK/CREB/BDNF signalings in vitro and in vivo models"

Genmeng, Yang,Juan, Li,Yanxia, Peng,Baoyu, Shen,Yuanyuan, Li,Liu, Liu,Chan, Wang,Yue, Xu,Shucheng, Lin,Shuwei, Zhang,Yi, Tan,Huijie, Zhang,Xiaofeng, Zeng,Qi, Li,Gang, Lu The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.2

L-arginine and N-carbamoylglutamic acid supplementation enhance young rabbit growth and immunity by regulating intestinal microbial community

Sun, Xiaoming,Shen, Jinglin,Liu, Chang,Li, Sheng,Peng, Yanxia,Chen, Chengzhen,Yuan, Bao,Gao, Yan,Meng, Xianmei,Jiang, Hao,Zhang, Jiabao Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1

Objective: An experiment was conducted to determine the effects of L-arginine (L-Arg) and N-carbamoylglutamic acid (NCG) on the growth, metabolism, immunity and community of cecal bacterial flora of weanling and young rabbits. Methods: Eighteen normal-grade male weanling Japanese White rabbits (JWR) were selected and randomly divided into 6 groups with or without L-Arg and NCG supplementation. The whole feeding process was divided into weanling stage (day 37 to 65) and young stage (day 66 to 85). The effects of L-Arg and NCG on the growth, metabolism, immunity and development of the ileum and jejunum were compared via nutrient metabolism experiments and histological assessment. The different communities of cecal bacterial flora affected by L-Arg and NCG were assessed using high-throughput sequencing technology and bioinformatics analysis. Results: The addition of L-Arg and NCG enhanced the growth of weanling and young rabbit by increasing the nitrogen metabolism, protein efficiency ratio, and biological value, as well as feed intake and daily weight gain. Both L-Arg and NCG increased the concentration of immunoglobulin A (IgA), IgM, and IgG. NCG was superior to L-Arg in promoting intestinal villus development by increasing villus height, villus height/crypt depth index, and reducing the crypt depth. The effects of L-Arg and NCG on the cecal bacterial flora were mainly concentrated in different genera, including Parabacteroides, Roseburia, dgA-11_gut_group, Alistipes, Bacteroides, and Ruminococcaceae_UCG-005. These bacteria function mainly in amino acid transport and metabolism, energy production and conversion, lipid transport and metabolism, recombination and repair, cell cycle control, cell division, and cell motility. Conclusion: L-Arg and NCG can promote the growth and immunity of weanling and young JWR, as well as effecting the jejunum and ileum villi. L-Arg and NCG have different effects in the promotion of nutrient utilization, relieving inflammation and enhancing adaptability through regulating microbial community.

Ginsenoside Rb1 attenuates methamphetamine (METH)-induced neurotoxicity through the NR2B/ERK/CREB/BDNF signalings in vitro and in vivo models

Genmeng Yang,Juan Li,Yanxia Peng,Baoyu Shen,Yuanyuan Li,Liu Liu,Chan Wang,Yue Xu,Shucheng Lin,Shuwei Zhang,Yi Tan,Huijie Zhang,Xiaofeng Zeng,Qi Li,Gang Lu 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.3

This study investigates the effects of ginsenoside Rb1 (GsRb1) on methamphetamine (METH)-induced toxicity in SH-SY5Y neuroblastoma cells and METH-induced conditioned place preference (CPP)in adult Sprague-Dawley rats. It also examines whether GsRb1 can regulate these effects through theNR2B/ERK/CREB/BDNF signaling pathways. Methods: SH-SY5Y cells were pretreated with GsRb1 (20 mM and 40 mM) for 1 h, followed by METHtreatment (2 mM) for 24 h. Rats were treated with METH (2 mg/kg) or saline on alternating days for 10days to allow CPP to be examined. GsRb1 (5, 10, and 20 mg/kg) was injected intraperitoneally 1 h beforeMETH or saline. Western blot was used to examine the protein expression of NR2B, ERK, P-ERK, CREB, PCREB, and BDNF in the SH-SY5Y cells and the rats' hippocampus, nucleus accumbens (NAc), and prefrontal cortex (PFC). Results: METH dose-dependently reduced the viability of SH-SY5Y cells. Pretreatment of cells with 40mM of GsRb1 increased cell viability and reduced the expression of METH-induced NR2B, p-ERK, p-CREBand BDNF. GsRb1 also attenuated the expression of METH CPP in a dose-dependent manner in rats. Further, GsRb1 dose-dependently reduced the expression of METH-induced NR2B, p-ERK, p-CREB, andBDNF in the PFC, hippocampus, and NAc of rats. Conclusion: GsRb1 regulated METH-induced neurotoxicity in vitro and METH-induced CPP through theNR2B/ERK/CREB/BDNF regulatory pathway. GsRb1 could be a therapeutic target for treating METHinduced neurotoxicity or METH addiction.

Removal of Cd(II) Ions from Wastewater by Compounding an O-Xanthogenated Chitosan Schiff Base and Fe(III) Ions

Kai Yang,Gang Wang,Fulong Liu,Yanxia Zhao 한국고분자학회 2019 Macromolecular Research Vol.27 No.10

The removal of Cd(II) ions from wastewater via the compound flocculation of O-xanthogenated chitosan Schiff base (XCTS) and Fe(III) ions was studied. The compound flocculant Fe(III)/XCTS was effective in an acid range of pH 3.0-5.0. When the pH was 3.0 and the compound ratio of [Fe(III)]/[XCTS] was 1:12, the compounded system Fe(III)/XCTS had the best effect on the removal of Cd(II) ions; the removal rate of Cd(II) ions was greater than 99.5%, and the Cd(II) ion concentration of the treated wastewater met the 0.1 mg L-1 limit set by the National Integrated Wastewater Discharge Standard of China. Furthermore, while studying the compound flocculation of Fe(III) ions and XCTS, we found that the approach enhanced the growth and structure of the flocs in the progress of removing Cd(II) ions.

Design Improvement of the Splitter Blade in the Centrifugal Pump Impeller Based on Theory of Boundary Vorticity Dynamics

Binjuan ZHAO,Chenghu ZHANG,Youfei ZHAO,Yanxia FU,Qi LIU,Huilong CHEN 한국유체기계학회 2018 International journal of fluid machinery and syste Vol.11 No.1

To improve the design of the splitter blade in the centrifugal pump impeller, inner flow numerical simulations are performed on the centrifugal pump impeller without splitter blades firstly, inner flow diagnoses are performed based on the theory of the boundary vorticity dynamics, and distributions of the boundary vorticity flux (BVF), friction force as well as vorticity on the inner walls of the impeller are carefully analyzed to find the location of bad flows and their dynamic sources. Later, according to the inner flow diagnosis results, splitter blades are designed and reformed for the original impeller. The inner flow filed in the impellers equipped with the preliminary splitter blade and reformed splitter blade are numerically simulated and diagnosed. Finally, comparisons are made among the three impellers, and it is found that, compared to the original impeller (impeller 0), the BVF, friction force and vorticity distribution in the impeller equipped with the preliminary splitter blade (impeller 1) has no obvious improvement, although the pump head increases, the efficiency decreases; the BVF, friction force and vorticity distribution in the impeller equipped with the reformed blade (impeller 2) has improved obviously, BVF peak decreases evidently and BVF distributes more uniformly, flow separation is inhibited, the hydraulic force acting on the impeller increases, with the pump head and efficiency increasing dramatically. It is proven that, flow diagnosis based on the theory of the boundary vorticity dynamics is an effective supplemental way for the design of the splitter blade in the centrifugal pump impeller, and will provide reference for the design of other turbo machinery as well.

Robust Design Optimization of Hydrodynamic Characteristics of Airfoil 791

Chenghu ZHANG,Binjuan ZHAO,Zhongfu HUANG,Yanxia FU,Qi LIU,Huilong CHEN 한국유체기계학회 2018 International journal of fluid machinery and syste Vol.11 No.3

In this paper, the influence of the continuous random variation of the flow velocity on hydrodynamic characteristics of airfoil 791 was discussed, and a robust design optimization method was proposed to reduce the influence of uncertain factors on the stability of hydrodynamic performance. Firstly, Bezier curve was used to parameterize the suction side of the airfoil, and its thickness was controlled by four points, which was then taken as the optimization variables. Secondly, the criterion of robustness was given, and then a robust mathematical model was established. Finally, two objective functions of robust optimization were gained on the basis of uncertainty analysis with surrogate model. Combined with multi-objective genetic algorithm, a robust optimal solution with better hydrodynamic characteristics was obtained. The results showed that compared with original case, the thickness near trailing edge altered more smoothly, the resistance and surface wave intensity were obviously decreased, and the maximum reduction of drag-lift ratio and wake unevenness were 6.89% and 25.04% respectively, which contributed to the improvement of wake quality. The change of surface pressure and resistance coefficients were also more stable under the variable inlet flow velocity conditions, and then the robustness was strengthened accordingly. In conclusion, the robust design optimization can obviously improve the hydrodynamic characteristics of the airfoil while reducing the sensitivity to uncertain factors, meanwhile, it can provide better stability during the operating process.