Distinct Pro-Apoptotic Properties of Zhejiang Saffron against Human Lung Cancer Via a Caspase-8-9-3 Cascade

Liu, Dan-Dan,Ye, Yi-Lu,Zhang, Jing,Xu, Jia-Ni,Qian, Xiao-Dong,Zhang, Qi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Lung cancer is the leading cause of cancer-related death worldwide. Here we investigated the antitumor effect and mechanism of Zhejiang (Huzhou and Jiande) saffron against lung cancer cell lines, A549 and H446. Using high performance liquid chromatography (HPLC), the contents of crocin I and II were determined. In vitro, MTT assay and annexin-V FITC/PI staining showed cell proliferation activity and apoptosis to be changed in a dose- and time-dependent manner. The inhibition effect of Jiande saffron was the strongest. In vivo, when mice were orally administered saffron extracts at dose of 100mg/kg/d for 28 days, xenograft tumor size was reduced, and ELISA and Western blotting analysis of caspase-3, -8 and -9 exhibited stronger expression and activity than in the control. In summary, saffron from Zhejiang has significant antitumor effects in vitro and in vivo through caspase-8-caspase-9-caspase-3 mediated cell apoptosis. It thus appears to have more potential as a therapeutic agent.

Factors related to the intention of healthy eating behaviors based on the theory of planned behavior: focused on adults residing in Beijing, China

Dan Liu,Seungwoo Lee,Ji-Yun Hwang 한국영양학회 2021 Journal of Nutrition and Health Vol.54 No.1

Purpose: The theory of planned behavior (TPB) was used to investigate how the psychological constructs of attitude, subjective norms, and perceived behavioral control (PBC) affect the individual intention of behaviors in adults. Social support is also important in enabling the stability of healthy eating. This study examined the relationship between three major constructs of TPB as well as social support and the intention of healthy dietary behaviors in adults residing in Beijing, China using the extended TPB. Methods: The study questionnaire was based on previously validated items and an online survey was conducted from October to November 2020. Using a total of 244 Chinese adults in Beijing, multiple linear regression analysis was used to test the relationships between three major constructs of TPB as well as the social support and intention of healthy eating. Results: Among the three major constructs of TPB, subjective norms (p = 0.044) and PBC (p = 0.000) were significantly related to the behavioral intention of healthy eating (p = 0.000), and the model explained 76.6% of the variance of the behavioral intention from the three constructs of TPB included in the multiple linear regression model. The additional inclusion of social support to the model did not increase the explanatory power of the model to describe the behavioral intention of healthy eating. The subjective norms (p = 0.040) and PBC (p = 0.000) were still significant where social support did not explain the variance of the behavioral intention adequately. Conclusion: The subjective norms and PBC may be potential determinants of the behavioral intention of healthy eating in adults residing in Beijing, China. These study results can be used to promote healthy eating in Chinese adults living in urban areas. Large-scale intervention studies will be needed to determine if social norms and PBC predict the actual behaviors of healthy eating in Chinese adults.

Application of Improved Ant Colony Algorithm in Solving TSP

Dan Liu,Lijuan Zheng,Jianmin Wang 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.7

A Measure Model for Synergy Degree between Technology Innovation and Institution Innovation and Its Empirical Analysis

Liu Dan,Yao Ping 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.10

Technological innovation and institutional innovation has a certain relative independence, at the same time, there is a co-evolution relationship between them, which interdependence and mutual promotion. In this paper, from the perspective of co-evolution analysis, Research on the relationship between technological innovation and institutional innovation in the process of resource-based city development, construct a measure model for synergy degree of compound system. A case study of Jixi city in resource based cities, analysis of the collaborative development of technological innovation and institutional innovation.

Oviposition decisions in an endoparasitoid under self-superparasitism conditions

Liu Peng-Cheng,Zhao Bin,Cao Dan-Dan,Wei Jian-Rong 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

Superparasitism is a widespread phenomenon. Having accepted superparasitism, mated female parasitoids must decide on the sex of each egg they subsequently lay into the same host. Theory predicts that this decision is either based on host quality, when more male eggs are laid in hosts that are already parasitized because they are perceived to be of poorer quality; or more eggs are laid of the sex that is most likely to be a strong larval competitor, i.e. generally females. Anastatus disparis is a facultative endoparasitic egg parasitoid. We used ‘artificial’ hosts to explore outcomes of decision making by A. disparis during superparasitism under a manipulated absence of larval competition. When only one egg was laid it was always female. As the number of eggs laid increased, so more of them were male. This supports the theory that oviposition decisions are based on host quality; more male eggs were laid in hosts that were already parasitized and thus of poorer quality. In a second experiment, eggs were exposed to parasitoids for different periods of time. Half the eggs were dissected to determine the number of parasitoid eggs that had been laid. The remaining eggs were incubated and the number and sex of offspring that ultimately emerged, following larval competition, were recorded. Under superparasitism conditions fierce larval competition ensued; only one offspring survived and they were pre dominantly female. In conclusion, oviposition decisions by female A. disparis accepting self-superparasitism were made based on host quality.

Analysis of Key Genes and Pathways Associated with Colorectal Cancer with Microarray Technology

Liu, Yan-Jun,Zhang, Shu,Hou, Kang,Li, Yun-Tao,Liu, Zhan,Ren, Hai-Liang,Luo, Dan,Li, Shi-Hong Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Objective: Microarray data were analyzed to explore key genes and their functions in progression of colorectal cancer (CRC). Methods: Two microarray data sets were downloaded from Gene Expression Omnibus (GEO) database and differentially expressed genes (DEGs) were identified using corresponding packages of R. Functional enrichment analysis was performed with DAVID tools to uncover their biological functions. Results: 631 and 590 DEGs were obtained from the two data sets, respectively. A total of 32 common DEGs were then screened out with the rank product method. The significantly enriched GO terms included inflammatory response, response to wounding and response to drugs. Two interleukin-related domains were revealed in the domain analysis. KEGG pathway enrichment analysis showed that the PPAR signaling pathway and the renin-angiotensin system were enriched in the DEGs. Conclusions: Our study to systemically characterize gene expression changes in CRC with microarray technology revealed changes in a range of key genes, pathways and function modules. Their utility in diagnosis and treatment now require exploration.

Microstructure and photoluminescence properties of Nd-doped Bi4Ti3O12 nanocrystalline

Dan Liu 한양대학교 세라믹연구소 2012 Journal of Ceramic Processing Research Vol.13 No.S1

Bi4-xNdxTi3O12 (x = 0.25, 0.5, 0.75, 1.00) nanocrystalline was prepared by modified sol-gel method. The powders crystallized well after annealing at 830 ο C for 30 min in air. Phase composition and optical properties of Bi4-xNdxTi3O12 nanocrystalline were investigated by X-ray diffraction (XRD), scanning electron microscope (SEM), photoluminescence (PL) spectra and ultraviolet-visible (UV-Vis) absorption spectra. In PL spectra, the major up-converted band observed at 534 nm (green) originated from the 4 G7/24 I9/2 excited state absorption (ESA) when exciting at 800 nm. It was also found that with increasing of x value the luminous intensity decreased because of different location of Nd3+ substitution. The optical energy band gap of the Bi3.5Nd0.5Ti3O12 nanocrystalline was found to be 3.17 eV by Tauc equation. The up-conversion mechanisms in the doped system will be discussed by analyzing the energy level structures of Nd3+.

RNA m5C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer

Liu Xiaoyi,Wei Qinglv,Yang Chenyue,Zhao Hongyan,Xu Jie,Mobet Youchaou,Luo Qingya,Yang Dan,Zuo Xinzhao,Chen Ningxuan,Yang Yu,Li Li,Wang Wei,Yu Jianhua,Xu Jing,Liu Tao,Yi Ping 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

5-Methylcytosine (m5C) is a common RNA modification that modulates gene expression at the posttranscriptional level, but the crosstalk between m5C RNA modification and biomolecule condensation, as well as transcription factor-mediated transcriptional regulation, in ovarian cancer, is poorly understood. In this study, we revealed that the RNA methyltransferase NSUN2 facilitates mRNA m5C modification and forms a positive feedback regulatory loop with the transcription factor E2F1 in ovarian cancer. Specifically, NSUN2 promotes m5C modification of E2F1 mRNA and increases its stability, and E2F1 binds to the NSUN2 promoter, subsequently reciprocally activating NSUN2 transcription. The RNA binding protein YBX1 functions as the m5C reader and is involved in NSUN2-mediated E2F1 regulation. m5C modification promotes YBX1 phase separation, which upregulates E2F1 expression. In ovarian cancer, NSUN2 and YBX1 are amplified and upregulated, and higher expression of NSUN2 and YBX1 predicts a worse prognosis for ovarian cancer patients. Moreover, E2F1 transcriptionally regulates the expression of the oncogenes MYBL2 and RAD54L, driving ovarian cancer progression. Thus, our study delineates a NSUN2-E2F1-NSUN2 loop regulated by m5C modification in a manner dependent on YBX1 phase separation, and this previously unidentified pathway could be a promising target for ovarian cancer treatment.

Piceatannol Inhibits Phorbol Ester-Induced NF-kappa B Activation and COX-2 Expression in Cultured Human Mammary Epithelial Cells.

Liu, Dan,Kim, Do-Hee,Park, Jong-Min,Na, Hye-Kyung,Surh, Young-Joon Lawrence Erlbaum Associates, Publishers [etc.] 2009 Nutrition and cancer Vol.61 No.6

<P>There are multiple lines of evidence supporting that inflammation is causally linked to carcinogenesis. Abnormal upregulation of cyclooxygenase-2 (COX-2), a rate-limiting enzyme in the prostaglandin biosynthesis, has been implicated in carcinogenesis. Trans-3,4,3',5'-tetrahydroxystilbene (piceatannol), a naturally occurring hydroxylated stilbene with potent anti-inflammatory and antioxidative activities, has been shown to inhibit the proliferation of several cancer cells by inducing apoptosis or blocking cell cycle progression. In this study, we examined the effect of piceatannol on activation of the nuclear transcription factor NF-kappa B, one of the major transcription factors that regulate proinflammatory COX-2 gene transcription, in human mammary epithelial (MCF-10A) cells treated with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). When pretreated to MCF-10A cells, piceatannol markedly inhibited TPA-induced NF-kappa B DNA binding to a greater extent than resveratrol and oxyresveratrol, stilbene analogs structurally related to piceatannol. Piceatannol also inhibited TPA-induced phosphorylation and degradation of Ikappa Balpha as well as nuclear translocation of the phosphorylated form of p65, the functionally active subunit of NF-kappa B. Likewise, TPA-induced expression of COX-2 was abrogated by piceatannol pretreatment. The thiol reducing agent dithiothreitol abolished the inhibitory effects of piceatannol on NF-kappa B DNA binding activity, suggesting that piceatannol may directly modify NF-kappa B or its regulator through reaction with the cysteine thiol(s).</P>

Effect of Temperature on Resistivity of CFRP Materials with Added Carbon Powder or Nano-silica

Dan Liu,Jie Yin,Rong-gui Liu,Shu Ping,Hui-guang Yin,Gui-hua Xie 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.4

This paper presents an experimental investigation on the effect of temperature on resistivity of Carbon Fiber Reinforced Polymer (CFRP) materials. A series of tests were conducted on three types of CFRP materials, namely pure CFRP material, CFRP with carbon powder (4% in weight) and CFRP with nano-silica (4% in weight). Test results showed that adding carbon powder into the epoxy resin decreases the initial electric resistance R0 and initial volume resistivity ρ0 while adding nano-silica increases R0 and ρ0 compared to pure CFRP material. Preheating cycle test results showed that the volume resistivity of all three types of specimens linearly increases with increasing temperature. CFRP with added nano-silica exhibits higher temperature sensitivity than CFRP with added carbon powder compared to the lowest temperature sensitivity for pure CFRP material. In addition, temperature cycle test results showed that CFRP specimens have approximately stable values of volume resistivity. Both CFRP specimens with added carbon powder or nano-silica exhibit a recognizable trend of first decrease and then increase in volume resistivity with increasing temperature both during heating and cooling cycles. CFRP with added carbon powder mainly shows Negative Temperature Coefficient (NTC) effect in the temperature range of −40 to 40oC and Positive Temperature Coefficient (PTC) effect from 40 to 80oC. CFRP with added nano-silica mainly exhibits PTC effect in the temperature domain of −15 to 80oC and NTC effect from −40 to −15oC. A mathematical-physical model with respect to the thermal effect was presented based on the Eshelby-Mori-Tanaka (EMT) approach and mesomechanics method. The results obtained with the model agree well with the test results considering the temperature domain of PTC effect, which indicates that the proposed model is effective in characterizing the variation of fractional change in resistance (ΔR/R0) at varying temperature.