LAMB3 is associated with disease progression and cisplatin cytotoxic sensitivity in head and neck squamous cell carcinoma

Liu, Lihua,Jung, Seung-Nam,Oh, Chan,Lee, Kyungmin,Won, Ho-Ryun,Chang, Jae Won,Kim, Jin Man,Koo, Bon Seok Elsevier 2019 European journal of surgical oncology Vol.45 No.3

<P><B>Abstract</B></P> <P><B>Objectives</B></P> <P>Laminin subunit beta-3 (LAMB3) is a major component of the basement membrane zone. In our study, we investigated the role of LAMB3 in head and neck squamous cell carcinoma (HNSCC) progression and its clinical implication as a prognostic biomarker.</P> <P><B>Materials and methods</B></P> <P>A retrospective analysis of 100 patients with HNSCC who had undergone curative surgery from 1999 to 2011 was performed. We evaluated LAMB3 expression by immunohistochemistry and its associations with clinicopathological characteristics and survival. For functional <I>in vitro</I> analyses, cell proliferation, migration, and invasion and western blot assays were performed following LAMB3 suppression. In addition, the role of LAMB3 in cisplatin-induced cytotoxicity was clarified by measuring cell proliferation.</P> <P><B>Results</B></P> <P>LAMB3 expression was up-regulated in HNSCC cell lines and patient tissues. High LAMB3 expression was significantly associated with positive lymph node metastasis (odds ratio: 6.316; <I>P</I> < 0.001) and poor prognosis in patients with HNSCC. LAMB3 suppression reduced cell migration/invasion via down-regulation of epithelial-to-mesenchymal transition-associated proteins (Vimentin and Slug). Moreover, LAMB3 suppression increased cisplatin cytotoxicity in HNSCC cells.</P> <P><B>Conclusion</B></P> <P>Our findings indicate that LAMB3 may be used as a prognostic biomarker in HNSCC and support that LAMB3 silencing could induce the sensitivity of anti-cancer drugs such as cisplatin.</P>

Pharmacokinetics, tissue distribution and excretion of peimisine in rats assessed by liquid chromatography-tandem mass spectrometry

Lihua Chen,Dongxun Li,Guosong Zhang,Wei Zhang,Lihua Zhang,Yongmei Guan,Weifeng Zhu,Hongning Liu 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

Peimisine, the common ingredient of ‘‘zhebeimu’’groups and ‘‘chuanbeimu’’ groups, is responsiblefor the expectorant and cough relieving effects. The aim ofthis study was to investigate the pharmacokinetics, tissuedistribution and excretion of peimisine in male and femaleSD (Sprague-Dawley) rats by a rapid and sensitive LC-MS/MS (liquid chromatography-tandem mass spectrometry)method used carbamazepine as the internal standard afteroral administration, carbamazepine was stated as an IS. The results showed that peimisine was slowly distributed,and eliminated from rat plasma and manifested lineardynamics in a dose range of 0.26–6.5 mg/kg. Tested byANOVA, there were gender differences in the pharmacokineticparameters of AUC0-t, AUC0-? among a singledose of 0.26, 1.3, 6.5 mg/kg (P\0.05). Drug blood andtissue levels in male rats were significantly higher than thefemale counterparts after oral administration, while boththe males and the females showed high drug levels inspleen, kidney, lung, liver and heart. On the other hand, thepeimisine levels that can be reached in uterus, ovary, testisand brain is low. The excretion study showed that littleadministered peimisine (\0.7 %) was recovered in themale and female bile. Approximately 13.46 and 15.05 %were recovered in female urine and feces, while 43.07 and7.49 % were recovered in male urine and feces, respectively,which indicated that the major elimination route ofmale rats was urine excretion. In addition, there was significantdifferences in total cumulative excretive ratio ofpeimisine in feces (P\0.05) and no significant differencesin the urine (P[0.05) at a dose of 1.3 mg/kg.

Tenascin-C Produced by Oxidized LDL-Stimulated Macrophages Increases Foam Cell Formation through Toll-like Receptor-4

Rui Liu,Lihua Zhang,Yong He,Bo Li,Jun Liu,Yingang Ren,Wei Han,Xing Wang 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.1

Atherosclerosis is a chronic inflammatory disease in which both innate and adaptive immunity are involved. Although there have been major advances in the involve-ment of toll-like receptor 4 (TLR4) and CD36 in the initiation and development of this disease, detailed mechanisms remain unknown. Here, we show that tenascin-C (TN-C) can stimulate foam cell formation and this can be inhibited by a TLR4-blocking antibody or CD36 gene silencing. Our results identify TN-C-TLR4 activation as a common molecular mechanism in oxLDL-stimulated foam cell formation and atherosclerosis. In addition, CD36 is the major scavenger receptor responsible for the TN-C-mediated foam cell formation. Taken together, we have identified that TN-C produced by oxLDL-stimulated macrophages increases foam cell formation through TLR4 and scavenger receptor CD36.

Characterization and expression analysis of BcAMT1;4, an ammonium transporter gene in flowering Chinese cabbage

Lihua Zhong,Xinmin Huang,Yunna Zhu,Erfeng Kou,Houcheng Liu,Guangwen Sun,Riyuan Chen,Shiwei Song 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.4

Ammonium (NH 4+ ) is generated during many endogenous metabolic processes in the leaves of plants, and there is increasing evidence that ammonium transporters (AMTs) play important roles in NH 4 + transmembrane transport and distribution. However, the expression of diff erent AMT genes is tissue-type specifi c and their functions diff er. Information about AMT genes and their expression under diff erent environmental conditions in fl owering Chinese cabbage ( Brassica campestris L.) is currently limited. Here, we isolated and characterized an AMT gene, BcAMT1;4 , in fl owering Chinese cabbage. BcAMT1;4 was localized to the plasma membrane and complemented NH 4+ transport in NH 4+ uptake-defi cient yeast. The highest expression levels of BcAMT1;4 were detected in the fl owers and leaves of fl owering Chinese cabbage. The expression of BcAMT1;4 was induced by nitrogen defi ciency and signifi cantly inhibited by the reapplication of NH 4+ (NH 4 Cl or NH 4 NO 3 ). In contrast, when plants pre-cultured in nitrate were transferred to an NH 4+ nutrient solution, BcAMT1;4 expression was signifi cantly enhanced. BcAMT1;4 exhibited a diurnal expression pattern, with higher expression levels during the light period than during the dark period, and a peak expression at the later stage of the light period. Knowledge of AMT genes in fl owering Chinese cabbage will lay a foundation for enhancing our understanding of the functional roles of diff erent AMT members in the regulation of its growth by NH 4+ , as BcAMT1;4 seems to play an important role in leaf NH 4+ transport.

The Feasibility Analysis of Recycled Aggregates from Construction and Demolition Waste for Permeable Pavement

Lihua Li,Xuanyu Shen,Jiabei Liu,Wentao Li,Chuheng Zhong,Xinlong Zhou 대한토목학회 2023 KSCE JOURNAL OF CIVIL ENGINEERING Vol.27 No.2

For reusing the construction and demolition waste (C&DW) materials and solving waterloggingproblems, the feasibility of applying C&DW to the permeable pavement (PP) was investigated. The recycled brick aggregate (RBA) and recycled concrete aggregate (RCA) were used as PPbase materials under different mixing ratios and grades. Geotextiles were also used in thisstudy as filter contaminants. Modified compaction tests, rainfall model tests, and columnleaching tests were conducted on a total of 15 samples with three gradations and five RBA andRCA mix ratios. The specimens for the rainfall model test were compacted at around optimummoisture content, and the degree of compaction was 97%. Using the sub-layer compactionmethod, each group of samples is compacted in five layers in strict accordance with thespecifications. In general, C&DW has the potential to be used underground without pollutionto the groundwater. The heavy metal concentrations in C&DW are less than in the local soiland related to material resources. The water yield performance was the best when 100%concrete was used to build PP, and the total water storage was the most when 100% of brickswere chosen to make PP. Due to the excellent permeability, C&DW can be selected forpavement fillers. The geotextile reduces the permeability by about 13% but effectively filtersout more than 50% of the fine particles in the rainfall. The permeability performance ofconcrete aggregates may be better than that of bricks, and fine particles can reduce thepermeability.

Mechanical Behavior of Reinforced Embankment with Different Recycling Waste Fillers

Lihua Li,Hongzhi Sheng,Henglin Xiao,Xinlong Zhou,Yiming Liu 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.8

The application of construction and demolition (C&D) waste and used tires in geotechnical engineering contributes to the demand of the sustainable development. This study mainly compared the mechanical behavior of pure sand embankment (PSE), C&D material embankment (CDME), sand-tire shreds mixture embankment (STSME) through a scale model test. Effects of tire shreds content, the first layer geocell reinforcement burial depth, geocell reinforcement depth and compaction degree on the bearing capacity of embankments were investigated. Moreover, embankment load-settlement ratio, the reinforced bearing capacity ratio, the bearing capacity improvement factor (IF), the embankment surface deformation, and the vertical earth pressure distribution inside the embankment were discussed. Results indicate that both C&D material fillers and sand-tire shreds mixture can improve the bearing capacity and stability of embankment slopes. However, C&D material is better than sand-tire shreds mixture in the improvement. The optimum value of tire shreds content is 5%. The reinforcement effect of geocell decreases with the increase of reinforcement depth. With the increase of the buried depth of the first layer, it first increases and then decreases. With the increase of compaction degree increases. The minimum earth pressure appears near the slope. The bearing capacity of CDME is greater than STSME. The ultimate bearing capacity of CDME under the action of the geocell is twice that of the unreinforced embankment.

Effects of Sperm Pretreatments and In vitro Culture Systems on Development of In vitro Fertilized Embryos Derived from Prepubertal Boer Goat Oocytes in China

Lv, Lihua,Yue, Wenbin,Liu, Wenzhong,Ren, Youshe,Li, Fuzhong,Lee, Kyung-Bon,Smith, George W. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.7

Use of oocytes from prepubertal animals for in vitro embryo production holds potential application for reducing generation intervals and increasing genetic progress through embryo transfer. The objective of these studies was to compare the effect of three sperm pretreatments (prior to in vitro fertilization) and seven embryo culture protocols on fertilization rate and (or) subsequent development of in vitro fertilized embryos derived from oocytes harvested from ovaries of 1-6 month old prepubertal Boer goats in China. Cleavage rates were highest for embryos fertilized with heparin-treated versus calcium ionophore- or caffeine-treated sperm. Similar rates of blastocyst development were observed using heparin- and ionophore-treated sperm, which were higher than obtained with caffeine-treated sperm. No differences in cleavage or blastocyst rates were observed following embryo culture in basal medias (synthetic oviductal fluid (SOF), Charles Rosenkrans 1 (CR1) or tissue culture medium-199 (TCM-199)) containing 10% fetal bovine serum (FBS). Cumulus or oviductal cell co-culture did not enhance cleavage or blastocyst rates relative to culture in SOF+10% FBS. Replacement of FBS in SOF medium with 0.3% BSA increased cleavage rates, but did not increase rates of blastocyst development. Sequential culture in SOF+0.3% BSA followed by SOF+10% FBS increased blastocyst yield versus continuous culture in SOF+10% FBS and tended to increase blastocyst yield versus continuous culture in SOF+0.3% BSA. These results demonstrate a pronounced effects of sperm pretreatments and in vitro embryo culture systems on rates of blastocyst development and provide a potential protocol (sperm pretreatment with heparin and sequential embryo culture in SOF+0.3% BSA followed by SOF+10% FBS) for generation of the significant numbers of in vitro produced blastocysts from oocytes of prepubertal Boer goats necessary for application of embryo transfer in rural regions of China for distribution of Boer goat genetics.

Tie1 contributes to the development of ovarian hyperstimulation syndrome under the regulation of EGR1 in granulosa cells

Sun Lihua,Tian Hui,Xue Songguo,Ye Hongjuan,Xue Xue,Wang Rongxiang,Liu Yu,Zhang Caixia,Chen Qiuju,Gao Shaorong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

The expression of tyrosine kinase with immunoglobulin-like and epidermal growth factor-like domains 1 (Tie1), a transmembrane protein expressed almost exclusively by endothelial cells, has been reported in granulosa cells. However, its significance in ovarian hyperstimulation syndrome (OHSS), which can occur after the injection of gonadotropins in infertile women undergoing controlled ovarian stimulation, is unknown. Here, we report significantly increased Tie1 and vascular endothelial growth factor (VEGF) expression in cultured granulosa cells from OHSS patients, as well as ovaries from rats with experimentally established OHSS, compared to controls, with the levels of both proteins also increasing in granulosa and SVOG cells (a nontumorigenic human granulosa-lutein cell line) treated with an acute dose of human chorionic gonadotropin (hCG). Tie1 silencing abolished the hCG-induced VEGF level in SVOG cells and attenuated the progression of OHSS in rats, as determined by histological analysis. Further studies in SVOG cells revealed that the hCG-induced upregulation of Tie1 expression involved the phosphoinositide 3-kinase/protein kinase B signaling pathway. We also report that early growth response protein 1 (EGR1), whose expression was also upregulated by hCG, bound directly to the Tie1 promoter and activated its transcription. Taken together, our results indicate that Tie1 may be a therapeutic target in cases of moderate-to-severe OHSS. Further studies are needed to address its clinical relevance.

Dynamic CT Myocardial Perfusion Imaging in Patients without Obstructive Coronary Artery Disease: Quantification of Myocardial Blood Flow according to Varied Heart Rate Increments after Stress

Yu Lihua,Tao Xiaofeng,Dai Xu,Liu Ting,Zhang Jiayin 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.1

Objective: The present study aimed to investigate the association between myocardial blood flow (MBF) quantified by dynamic CT myocardial perfusion imaging (CT-MPI) and the increments in heart rate (HR) after stress in patients without obstructive coronary artery disease. Materials and Methods: We retrospectively included 204 subjects who underwent both dynamic CT-MPI and coronary CT angiography (CCTA). Patients with more than minimal coronary stenosis (diameter ≥ 25%), history of myocardial infarction/ revascularization, cardiomyopathy, and microvascular dysfunction were excluded. Global MBF at stress was measured using hybrid deconvolution and maximum slope model. Furthermore, the HR increments after stress were recorded. Results: The median radiation dose of dynamic CT-MPI plus CCTA was 5.5 (4.5–6.8) mSv. The median global MBF of all subjects was 156.4 (139.8–180.4) mL/100 mL/min. In subjects with HR increment between 10 to 19 beats per minute (bpm), the global MBF was significantly lower than that of subjects with increment between 20 to 29 bpm (153.3 mL/100 mL/min vs. 171.3 mL/100 mL/min, p = 0.027). This difference became insignificant when the HR increment further increased to ≥ 30 bpm. Conclusion: The global MBF value was associated with the extent of increase in HR after stress. Significantly higher global MBF was seen in subjects with HR increment of ≥ 20 bpm.

Microstructures and Thermal Properties of Polycaprolactone,Epoxy Resin,SiO2 Hybrids

( He Lihua ),( Liu Pinggui ),( Ding Heyan ) 한국 접착 및 계면학회 2006 접착 및 계면 Vol.7 No.4