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      • KCI등재

        Low Temperature during Seedling Stage Promotes Female Flower Determination but Not Yield of Chieh-qua

        Shiwei Song,Hongxing Huang,Houcheng Liu,Guangwen Sun,Riyuan Chen 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.5

        Temperature has an important effect on plant growth and sex determination in flowers. The effect of different temperatures (high temperature: 30 /25 (day/night), medium temperature: 25 /18 and low temperature: 15 /10 )on flower sex determination and fruit yield was studied in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How. ‘No. 4 Jiang xin’), also the content of absicsic acid (ABA) and indole acetic acid (IAA) in the shoot apex was measured. The results showed that medium and low temperatures during seedling stage boost the female flower differentiation after transplanting, compared with high temperature treatment, and the effect was more obvious when the temperature was lower. Lower temperature increased the content of ABA and IAA in shoot apex of Chieh-qua. There was a positive relationship between the ratio of IAA/ABA and plant female flower determination under different temperature treatments (R = 0.8744). Temperature may affect female flower differentiation of Chieh-qua by changing the contents of ABA and IAA in shoot apex. Fruit yield was not significantly affected by different temperature treatments.

      • KCI등재

        Cloning and Characterization of the Ammonium Transporter Genes BaAMT1;1 and BaAMT1;3 from Chinese Kale

        Shiwei Song,Riyuan Chen,Zhenhua He,Xinmin Huang,Lihua Zhong,Houcheng Liu,Guangwen Sun 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.2

        Chinese kale (Brassica alboglabra L.) is a popular vegetable rich in important nutrients. Fertilization withappropriate ammonium:nitrate ratios enhances biomass production and quality. AMT-type ammonium transporters havebeen shown to mediate ammonium uptake across the plasma membrane. However, very little is known about themolecular regulation of growth and development by ammonium in Chinese kale, including how ammonium regulatesthe expression of AMT1 genes. In this study, we identified and characterized two AMT1 genes from B. alboglabra,BaAMT1;1 and BaAMT1;3. The full-length open reading frames of BaAMT1;1 and BaAMT1;3 were 1512 bp and1515 bp, respectively. Transient expression of the fusion proteins pBE-EGFP-BaAMT1;1 and pBE-EGFP-BaAMT1;3 inonion epidermal cells indicated that these transporters are located on the plasma membrane. BaAMT1;1 and BaAMT1;3were functional in yeast and complemented a mutant defective in ammonium transport. BaAMT1;1 was expressed invegetative organs and at high levels in roots, while BaAMT1;3 expression was root specific. In addition, we observedopposite responses of BaAMT1;1 and BaAMT1;3 expression to nitrogen starvation and ammonium resupply in roots. These results provide new insights into the molecular mechanisms underlying ammonium absorption in Chinese kale.

      • KCI등재

        Phosphorus Deficiency Restricts Plant Growth but Induces Pigment Formation in the Flower Stalk of Chinese Kale

        Riyuan Chen,Shiwei Song,Xiuchun Li,Houcheng Liu,Danfeng Huang 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.3

        The effect of phosphorus (P) nutrition on plant growth and pigment formation in the flower stalk was studied under hydroponic conditions for 2 Chinese kale (Brassica alboglabra Bailey) cultivars: ‘Jianyexia’ (green flower stalk)and ‘Hongjiao’ (mauve flower stalk). Three different P treatments were used: 30 (normal-P), 7.5 (low-P), and 0 mg·L-1(P-deficient). The results showed that the biomass, yield, plant height, stem diameter, and leaf number of Chinese kale were significantly reduced in the low-P and P-deficient treatments compared to the normal-P treatment. The chlorophyll content in the flower stalk epidermis was not affected by different P levels in ‘Jianyexia’, but was significantly reduced by the P-deficient treatment in ‘Hongjiao’. Decreased P levels caused the flavonoid, soluble phenol, and anthocyanin content of the flower stalks to gradually increase in both Chinese kale cultivars. The pH value of the flower stalk epidermis gradually decreased with the declining P levels, and was significantly different among the 3treatments. As the P levels declined, phenylalanine ammonia-lyase (PAL) and chalcone isomerase (CHI) activities in the flower stalk epidermis gradually increased, and were significantly different among the 3 treatments. P nutrition may control the synthesis of anthocyanins in the flower stalk by regulating the epidermal pH value, and the activities of PAL and CHI.

      • KCI등재

        Fucosylated Chondroitin Sulfate from Sea Cucumber Inhibited Islets of Langerhans Apoptosis via Inactivation of the Mitochondrial Pathway in Insulin Resistant Mice

        Wei Jiang,Shijie Li,Shiwei Hu,Jignfeng Wang,Wendong Song,Lili Ji,Yaning Wang 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.3

        The effects of fucosylated chondroitin sulphate from sea cucumber (CHS) on inhibition of pancreatic islet apoptosis in high-fat high-sucrose diet (HFSD)-induced insulin resistant mice were studied for 19 weeks. CHS treatment significantly (p<0.05) decreased fasting blood glucose, insulin, and tumor necrosis factor (TNF)-α levels, and increased the serum adiponectin level, improved insulin resistance, and repaired HFSD-injured islets of langerhans, compared with controls. CHS supplementation significantly (p<0.05) inhibited pancreas cell apoptosis via reduction of cytochrome c, caspase 9, and caspase 3 mRNA expressions, cytochrome c release into the cytoplasm, and caspase 9 and cleaved-caspase 3 protein expressions, compared with controls. These effects were associated with upregulation of Bcl-2 and Bcl-xL mRNA and protein expressions, and downregulation of Bax and t-Bid mRNA and protein expressions. Combination treatments with CHS and rosiglitazone synergistically protected pancreatic islets. CHS improves insulin resistance and protects islets of langerhans from apoptosis via inactivation of the intrinsic mitochondrial pathway.

      • KCI등재

        Laplacian-Regularized Mean Apparent Propagator-MRI in Evaluating Corticospinal Tract Injury in Patients with Brain Glioma

        Jiang Rifeng,Jiang Shaofan,Song Shiwei,Wei Xiaoqiang,Deng Kaiji,Zhang Zhongshuai,Xue Yunjing 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.5

        Objective: To evaluate the application of laplacian-regularized mean apparent propagator (MAPL)-MRI to brain glioma-induced corticospinal tract (CST) injury. Materials and Methods: This study included 20 patients with glioma adjacent to the CST pathway who had undergone structural and diffusion MRI. The entire CSTs of the affected and healthy sides were reconstructed, and the peritumoral CSTs were manually segmented. The morphological characteristics of the CST (track number, average length, volume, displacement of the affected CST) were examined and the diffusion parameter values, including fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), radial diffusivity (RD), mean squared displacement (MSD), q-space inverse variance (QIV), returnto- origin probability (RTOP), return-to-axis probabilities (RTAP), and return-to-plane probabilities (RTPP) along the entire and peritumoral CSTs, were calculated. The entire and peritumoral CST characteristics of the affected and healthy sides as well as those relative CST characteristics of the patients with motor weakness and normal motor function were compared. Results: The track number, volume, MD, RD, MSD, QIV, RTAP, RTOP, and RTPP of the entire and peritumoral CSTs changed significantly for the affected side, whereas the AD and FA changed significantly only in the peritumoral CST (p < 0.05). In patients with motor weakness, the relative MSD of the entire CST, QIV of the entire and peritumoral CSTs, and the AD, MD, RD of the peritumoral CST were significantly higher, whereas the RTPP of the entire and peritumoral CSTs and the RTOP of the peritumoral CST were significantly lower than those in patients with normal motor function (p < 0.05 for all). In contrast, no significant changes were found in the CST morphological characteristics, FA, or RTAP (p > 0.05 for all). Conclusion: MAPL-MRI is an effective approach for evaluating microstructural changes after CST injury. Its sensitivity may improve when using the peritumoral CST features.

      • KCI등재

        Effect of supplemental blue light intensity on the growth and quality of Chinese kale

        Yamin Li,Yinjian Zheng,Houcheng Liu,Yiting Zhang,Yanwei Hao,Shiwei Song,Bingfu Lei 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.1

        The infl uence of blue light on the growth and quality of vegetables is controversial, since both positive and negative eff ectshave been observed on diff erent varieties of vegetables and most research has been conducted with a combination of lightspectrums. In this work, we investigated the eff ects of supplemental blue light intensity on growth, nutritional quality, andhealth-promoting compounds of Chinese kale ( Brassica alboglabra Bailey) using only blue (460 nm) light-emitting diodes10 days before harvest in a greenhouse under ambient light conditions. Four blue-light treatments were conducted (0, 50,100, and 150 μmol m −2 s −1 , named T0, T50, T100, and T150, respectively) with 12 h (6:00–18:00) of light treatment perday. The growth of Chinese kale plants was improved by supplemental blue light. The fresh and dry weights of Chinesekale plants were signifi cantly higher under T50 and T100 than those in the other treatments. The content of chlorophyll band total chlorophyll increased signifi cantly under T50, and the content of carotenoids increased signifi cantly under T150. The contents of soluble sugars and free amino acids were signifi cantly increased under the blue light treatments. The nitratecontent decreased with increasing blue light intensity, while the anthocyanin content increased. The contents of vitamin Cin T50 and total phenolic compounds in T150 were signifi cantly higher than in T0. The fl avonoid contents were signifi canthigher in T50 and T150, and signifi cantly lower in T100. Our results show that blue light can infl uence the growth and qualityof Chinese kale and 50 μmol m −2 s −1 of supplemental blue light might be the most feasible light intensity for Chinesekale production.

      • KCI등재후보

        Influence of Solution Concentrations on Surface Morphology and Wettability of ZnO Thin Films

        Jianguo Lv,Changlong Liu,Feng Wang,Zhitao Zhou,Zhenfa Zi,Yuan Feng,Xiaoshuang Chen,Feng Liu,Gang He,Shiwei Shi,Xueping Song,Zhaoqi Sun 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.2

        ZnO thin films were grown on silicon substrates using a hydrothermal method. The XRD patterns show that all of the peaks can be attributed to the wurtzite structures of ZnO. The TC value of (002) plane and average crystal size increase first and then decrease with the increase of solution concentration. SEM and AFM results show that many dense hexagonal cylinder particles have been observed on the surface of the thin films, which grown at 0.08 and 0.10 mol/L. The surface roughness of the thin films deposited at 0.06,0.08, 0.10, and 0.12 mol/L are 24.5, 38.3, 32.0, and 39.4 nm, respectively. Surface wettability results show that the preferential orientation along c-axis and surface roughness contribute significantly to the hydrophobicity. The reversible switching between hydrophobicity and hydrophilicity is related to the synergy of the transition of wetting model, surface crystal structure, and surface roughness.

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