The cooperative regulatory effect of the miRNA-130 family on milk fat metabolism in dairy cows

Li Xiaofen,Wu Yanni,Yang Xiaozhi,Gao Rui,LUQINYUE,Lv Xiaoyang,Chen Zhi 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.7

Objective: There is a strong relationship between the content of beneficial fatty acids in milk and milk fat metabolic activity in the mammary gland. To improve milk quality, it is therefore necessary to study fatty acid metabolism in bovine mammary gland tissue. In adipose tissue, peroxisome proliferator-activated receptor gamma (<i>PPARG</i>), the core transcription factor, regulates the fatty acid metabolism gene network and determines fatty acid deposition. However, its regulatory effects on mammary gland fatty acid metabolism during lactation have rarely been reported.Methods: Transcriptome sequencing was performed during the prelactation period and the peak lactation period to examine mRNA expression. The significant upregulation of <i>PPARG</i> drew our attention and led us to conduct further research.Results: According to bioinformatics prediction, dual-luciferase reporter system detection, real-time quantitative reverse transcription polymerase chain reaction and Western blotting, miR-130a and miR-130b could directly target <i>PPARG</i> and inhibit its expression. Furthermore, triglyceride and oil red O staining proved that miR-130a and miR-130b inhibited milk fat metabolism in bovine mammary epithelial cells (BMECs), while <i>PPARG</i> promoted this metabolism. In addition, we also found that the coexpression of miR-130a and miR-130b significantly enhanced their ability to regulate milk fat metabolism.Conclusion: In conclusion, our findings indicated that miR-130a and miR-130b could target and repress PPARG and that they also have a functional superposition effect. miR-130a and miR-130b seem to synergistically regulate lipid catabolism via the control of PPARG in BMECs. In the long-term, these findings might be helpful in developing practical means to improve high-quality milk. Objective: There is a strong relationship between the content of beneficial fatty acids in milk and milk fat metabolic activity in the mammary gland. To improve milk quality, it is therefore necessary to study fatty acid metabolism in bovine mammary gland tissue. In adipose tissue, peroxisome proliferator-activated receptor gamma (PPARG), the core transcription factor, regulates the fatty acid metabolism gene network and determines fatty acid deposition. However, its regulatory effects on mammary gland fatty acid metabolism during lactation have rarely been reported. Methods: Transcriptome sequencing was performed during the prelactation period and the peak lactation period to examine mRNA expression. The significant upregulation of PPARG drew our attention and led us to conduct further research. Results: According to bioinformatics prediction, dual-luciferase reporter system detection, real-time quantitative reverse transcription polymerase chain reaction and Western blotting, miR-130a and miR-130b could directly target PPARG and inhibit its expression. Furthermore, triglyceride and oil red O staining proved that miR-130a and miR-130b inhibited milk fat metabolism in bovine mammary epithelial cells (BMECs), while PPARG promoted this metabolism. In addition, we also found that the coexpression of miR-130a and miR-130b significantly enhanced their ability to regulate milk fat metabolism. Conclusion: In conclusion, our findings indicated that miR-130a and miR-130b could target and repress PPARG and that they also have a functional superposition effect. miR130a and miR-130b seem to synergistically regulate lipid catabolism via the control of PPARG in BMECs. In the long-term, these findings might be helpful in developing practical means to improve high-quality milk.

Mutations of ARX and non-syndromic intellectual disability in Chinese population

Yufei Wu,Huan Zhang,Xiaofen Liu,Zhangyan Shi,Hongling Li,Zhibin Wang,Xiaoyong Jie,Shao-Ping Huang,Fu-Chang Zhang,Junlin Li,Ke-Jin Zhang,Xiao-Cai Gao 한국유전학회 2019 Genes & Genomics Vol.41 No.1

Mutations of Aristaless-related homeobox (ARX) gene were looked as the third cause of non-syndromic intellectual disability (NSID), while the boundary between true disease-causing mutations and non-disease-causing variants within this gene remains elusive. To investigate the relationship between ARX mutations and NSID, a panel comprising six reported causal mutations of the ARX was detected in 369 sporadic NSID patients and 550 random participants in Chinese. Two mutations, c.428_451 dup and p.G286S, may be disease-causing mutations for NSID, while p.Q163R and p.P353L showed a great predictive value in female NSID diagnosis with significant associations (X2 = 19.60, p = 9.54e−6 for p.Q163R; X2 = 25.70, p = 4.00e−07 for p.P353L), carriers of these mutations had an increased risk of NSID of more than fourfold. Detection of this panel also predicted significant associations between genetic variants of the ARX gene and NSID (p = 3.73e−4). The present study emphasized the higher genetic burden of the ARX gene on NSID in the Chinese population, molecular analysis of this gene should be considered for patients presenting NSID of unknown etiology.

Characterization and Expression Profile Analysis of a New cDNAEncoding Taxadiene Synthase from Taxus media

Lingxia Zhao,Binhui Guo,Xiaofen Sun,Guoyin Kai,Lei Zhang,Zhugang Li,Dongli Zhao,Zhiqi Miao,Kexuan Tang 한국생화학분자생물학회 2005 BMB Reports Vol.38 No.6

Isolation and Molecular Characterization of a New CRT Binding Factor Gene from Capsella bursa-pastoris

Wang, Xinglong,Liu, Li,Liu, Sixiu,Sun, Xiaoqing,Deng, Zhongxiang,Pi, Yan,Sun, Xiaofen,Tang, Kexuan Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.5

A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella bursa-pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbf1, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbf16, and Bncbf17 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.

Characterization and Expression Profile Analysis of a New cDNA Encoding Taxadiene Synthase from Taxus media

Kai, Guoyin,Zhao, Lingxia,Zhang, Lei,Li, Zhugang,Guo, Binhui,Zhao, Dongli,Sun, Xiaofen,Miao, Zhiqi,Tang, Kexuan Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6

A full-length cDNA encoding taxadiene synthase (designated as TmTXS), which catalyzes the first committed step in the Taxol biosynthetic pathway, was isolated from young leaves of Taxus media by rapid amplification of cDNA ends (RACE). The full-length cDNA of TmTXS had a 2586 bp open reading frame (ORF) encoding a protein of 862 amino acid residues. The deduced protein had isoelectric point (pI) of 5.32 and a calculated molecular weight of about 98 kDa, similar to previously cloned diterpene cyclases from other Taxus species such as T. brevifolia and T. chinenisis. Sequence comparison analysis showed that TmTXS had high similarity with other members of terpene synthase family of plant origin. Tissue expression pattern analysis revealed that TmTXS expressed strongly in leaves, weak in stems and no expression could be detected in fruits. This is the first report on the mRNA expression profile of genes encoding key enzymes involved in Taxol biosynthetic pathway in different tissues of Taxus plants. Phylogenetic tree analysis showed that TmTXS had closest relationship with taxadiene synthase from T. baccata followed by those from T. chinenisis and T. brevifolia. Expression profiles revealed by RT-PCR under different chemical elicitor treatments such as methyl jasmonate (MJ), silver nitrate (SN) and ammonium ceric sulphate (ACS) were also compared for the first time, and the results revealed that expression of TmTXS was all induced by the tested three treatments and the induction effect by MJ was the strongest, implying that TmTXS was high elicitor responsive.

An Efficient Broadcast Authentication Scheme with Batch Verification for ADS-B Messages

( Haomiao Yang ),( Hyunsung Kim ),( Hongwei Li ),( Eunjun Yoon ),( Xiaofen Wang ),( Xuefeng Ding ) 한국인터넷정보학회 2013 KSII Transactions on Internet and Information Syst Vol.7 No.10

As a cornerstone of the next generation air traffic management (ATM), automatic dependent surveillance-broadcast (ADS-B) system can provide continual broadcast of aircraft position, identity, velocity and other messages over unencrypted data links to generate a common situational awareness picture for ATM. However, since ADS-B messages are unauthenticated, it is easy to insert fake aircrafts into the system via spoofing or insertion of false messages. Unfortunately, the authentication for ADS-B messages has not yet been well studied. In this paper, we propose an efficient broadcast authentication scheme with batch verification for ADS-B messages which employs an identity-based signature (IBS). Security analysis indicates that our scheme can achieve integrity and authenticity of ADS-B messages, batch verification, and resilience to key leakage. Performance evaluation demonstrates that our scheme is computationally efficient for the typical avionics devices with limited resources, and it has low communication overhead well suitable for low-bandwidth ADS-B data link.

Cloning and Functional Analysis of a cDNA Encoding Ginkgo biloba Farnesyl Diphosphate Synthase

Kexuan Tang,Peng Wang,Zhihua Liao,Liang Guo,Wenchao Li,Min Chen,Yan Pi,Yifu Gong,Xiaofen Sun 한국분자세포생물학회 2004 Molecules and cells Vol.18 No.2

Farnesyl diphosphate synthase (FPS; EC2.5.1.1/EC2. 5.1.10) catalyzes the synthesis of farnesyl diphosphate, and provides precursor for biosynthesis of sesquiterpene and isoprenoids containing more than 15 isoprene units in Ginkgo biloba. Here we report the cloning, characterization and functional analysis of a new cDNA encoding FPS from G. biloba. The full-length cDNA (designated GbFPS) had 1731 bp with an open reading frame of 1170 bp encoding a polypeptide of 390 amino acids. The deduced GbFPS was similar to other known FPSs and contained all the conserved regions of trans-prenyl chain-elongating enzymes. Structural modeling showed that GbFPS had the typical structure of FPS, the most prominent feature of which is the arrangement of 13 core helices around a large central cavity. Southern blot analysis revealed a small FPS gene family in G. biloba. Expression analysis indicated that GbFPS expression was high in roots and leaves, and low in stems. Functional complementation of GbFPS in an FPS-deficient strain confirmed that GbFPS mediates farnesyl diphosphate biosynthesis.

The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability

Yang Qianqian,Yan Ding,Zou Chaoying,Xue Qian,Lin Shuhui,Huang Qingtian,Li Xiaofen,Tang Daolin,Chen Xin,Liu Jinbao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Triple-negative breast cancer (TNBC) is a heterogeneous malignancy in women. It is associated with poor prognosis, aggressive malignant behavior, and limited treatment options. In the ubiquitin‒proteasome system (UPS), deubiquitinases (DUBs) are potential therapeutic targets for various tumors. In this study, by performing unbiased siRNA screening, we identified STAMBP, a JAMM metalloprotease in the DUB family, as a driver of human TNBC tumor growth. Functionally, the knockdown of STAMBP inhibited the proliferation, migration, and invasion of multiple TNBC cell lines. Immunoprecipitation–mass spectrometry combined with functional and morphological analysis verified the interaction between STAMBP and the actin-binding protein RAI14. Mechanistically, STAMBP stabilized the RAI14 protein by suppressing the K48-linked ubiquitination of RAI14 and thus prevented its proteasomal degradation. Therefore, knocking down STAMBP resulted in the reduction in RAI14 protein levels and suppression of tumor growth in vitro and in vivo. Importantly, high levels of STAMBP were correlated with poor prognosis in TNBC patients. In summary, we reveal a previously unrecognized DUB pathway that promotes TNBC progression and provides a rationale for potential therapeutic interventions for the treatment of TNBC.

Enhanced electrochemical performance of Ca-doped NdBa_1-xCa_xCoCuO_5+δ as cathode material for intermediate-temperature solid oxide fuel cells

Pang, Shengli,Su, Yanjing,Yang, Gongmei,Shen, Xiangqian,Zhu, Meng,Wu, Xiao,Li, Songwei,Yang, Xiaofen,Xi, Xiaoming Elsevier 2018 Ceramics international Vol.44 No.17

<P><B>Abstract</B></P> <P>Exploring advanced cathode materials that have high electrochemical activity and superior thermal compatibility with other components is of great importance for the application of intermediate-temperature solid oxide fuel cells. In the present study, influences of Ca doping on electrochemical and physicochemical properties of NdBa<SUB>1-x</SUB>Ca<SUB>x</SUB>CoCuO<SUB>5+δ</SUB> were studied systematically. A relatively low thermal expansion coefficient (16.8 × 10<SUP>−6</SUP> °C<SUP>−1</SUP>) was obtained for the Ca-doped sample over the temperature range of 25–800 °C. Moreover, Ca doping significantly enhanced the electrochemical performance of NdBa<SUB>1-x</SUB>Ca<SUB>x</SUB>CoCuO<SUB>5+δ</SUB>. The highest densities of power for NdBa<SUB>1-x</SUB>Ca<SUB>x</SUB>CoCuO<SUB>5+δ</SUB>-based single cells at the temperatures of 800 °C increased from 1.42 W cm<SUP>−2</SUP> for x = 0.0 to 1.84 W cm<SUP>−2</SUP> for x = 0.3. This increase in electrochemical performance of NdBa<SUB>1-x</SUB>Ca<SUB>x</SUB>CoCuO<SUB>5+δ</SUB> cathode with Ca doping can be ascribed to enhanced concentrations of oxygen vacancies and higher electrical conductivity.</P>

Molecular Cloning, Characterization and Functional Analysis of a 2C-methyl-D-erythritol 2, 4-cyclodiphosphate Synthase Gene from Ginkgo biloba

Gao, Shi,Lin, Juan,Liu, Xuefen,Deng, Zhongxiang,Li, Yingjun,Sun, Xiaofen,Tang, Kexuan Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.5

2C-methyl-D-erythritol 2, 4-cyclodiphosphate synthase (MECPS, EC: 4.6.1.12) is the fifth enzyme of the non-mevalonate terpenoid pathway for isopentenyl diphosphate biosynthesis and is involved in the methylerythritol phosphate (MEP) pathway for ginkgolide biosynthesis. The full-length mecps cDNA sequence (designated as Gbmecps) was cloned and characterized for the first time from gymnosperm plant species, Ginkgo biloba, using RACE (rapid amplification of cDNA ends) technique. The full-length cDNA of Gbmecps was 874 bp containing a 720 bp open reading frame (ORF) encoding a peptide of 239 amino acids with a calculated molecular mass of 26.03 kDa and an isoelectric point of 8.83. Comparative and bioinformatic analyses revealed that GbMECPS showed extensive homology with MECPSs from other species and contained conserved residues owned by the MECPS protein family. Phylogenetic analysis indicated that GbMECPS was more ancient than other plant MECPSs. Tissue expression pattern analysis indicated that GbMECPS expressed the highest in roots, followed by in leaves, and the lowest in seeds. The color complementation assay indicated that GbMECPS could accelerate the accumulation of $\beta$-carotene. The cloning, characterization and functional analysis of GbMECPS will be helpful to understand more about the role of MECPS involved in the ginkgolides biosynthesis at the molecular level.