Early IL-17A Prevention Rather Than Late IL-17A Neutralization Attenuates Toluene Diisocyanate-Induced Mixed Granulocytic Asthma

Chen Shuyu,Yu Li,Deng Yao,Liu Yuanyuan,Wang Lingwei,Li Difei,Yang Kai,Liu Shengming,Tao Ailin,Chen Rongchang 대한천식알레르기학회 2022 Allergy, Asthma & Immunology Research Vol.14 No.5

Purpose: Interleukin (IL)-17A plays a critical role in the pathogenesis of allergic airway inflammation. Yet, the exact roles of IL-17A in asthma are still controversial. Thus, the aim of this study was to dissect the roles of IL-17A in toluene diisocyanate (TDI)-induced mixed granulocytic asthma and to assess the effects of neutralizing antibody in different effector phases on TDI-induced asthma. Methods: IL-17A functions in allergic airway inflammation were evaluated using mice deficient in IL-17A (Il17a−/−) or IL-17A monoclonal antibody (IL-17A mab, intraperitoneally, 50 μg per mouse, 100 μg per mouse). Moreover, the effects of exogenous recombinant IL (rIL)-17A in vivo (murine rIL-17A, intranasally, 1 μg per mouse) and in vitro (human rIL-17A, 100 ng/mL) were investigated. Results: TDI-induced mixed granulocytic airway inflammation was IL-17A-dependent because airway hyperreactivity, neutrophil and eosinophil infiltration, airway smooth muscle thickness, epithelium injury, dysfunctional T helper (Th) 2 and Th17 responses, granulocytic chemokine production and mucus overproduction were more markedly reduced in the Il17a−/− mice or by IL-17A neutralization during the sensitization phase of wild-type (WT) mice. By contrast, IL-17A neutralization during the antigen-challenge phase aggravated TDI-induced eosinophils recruitment, with markedly elevated Th2 response. In line with this, instillation of rIL-17 during antigen sensitization exacerbated airway inflammation by promoting neutrophils aggregation, while rIL-17A during the antigen-challenge phase protected the mice from TDI-induced airway eosinophilia. Moreover, rIL-17A exerted distinct effects on eosinophil- or neutrophil-related signatures in vitro. Conclusions: Our data demonstrated that IL-17A was required for the initiation of TDI-induced asthma, but functioned as a negative regulator of established allergic inflammation, suggesting that early abrogation of IL-17A signaling, but not late IL-17A neutralization, may prevent the progression of TDI-induced asthma and could be used as a therapeutic strategy for severe asthmatics in clinical settings.

Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny

Han, Hongmei,Wang, Aihong,Liu, Liming,Zhao, Gaoping,Su, Jie,Wang, Biao,Li, Yunxia,Zhang, Jindun,Wu, Baojiang,Sun, Wei,Hu, Shuxiang,Li, Shuyu,Zhao, Lixia,Li, Xihe Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.6

Most hinnies (female donkey${\times}$male horse) and mules (female horse${\times}$male donkey) are sterile with few reports of equine fertile hybrids. The main cause of this sterility is thought to be a meiotic block to spermatogenesis and oogenesis. This study compared the developmental features of the testes and a histological analyses of spermatogenesis in a male hinny with those of a normal, fertile stallion and Jack donkey. Hinny testes showed a thicker tunica albuginea, fewer blood vessels and more connective tissue in the testis parenchyma than those of the stallion and Jack donkey. Although the mean number of seminiferous tubules was significantly higher in stallion and hinny than Jack donkey (p<0.01), the mean proportion of seminiferous tubules was lower in the hinny (p<0.01) which resulted in a smaller diameter of seminiferous tubules. The mean number of spermatogonia and spermatocytes per unit area were significantly lower in hinny testis (p<0.01) and no spermatids or mature spermatozoa cells were found during immunofluorescent analyses. These results indicated that defects in seminiferous tubule development and structure occur in the testis of hinnies. Furthermore, most spermatogonia and spermatocytes cease development in synapsis during mid-meiosis of spermatocytes, which results in a block to spermatogenesis that prevents the formation of spermatids and matured spermatozoa during meiosis in male hinnies.

Building Asian climate change scenario by multi‐regional climate models ensemble. Part II: mean precipitation

Li, Qian,Wang, Shuyu,Lee, Dong‐,Kyou,Tang, Jianping,Niu, Xiaorui,Hui, Pinhong,Gutowski Jr., William J.,Dairaku, Koji,McGregor, John L.,Katzfey, Jack,Gao, Xuejie,Wu, Jia,Hong, Song‐,You,Wan Wiley 2016 International journal of climatology Vol.36 No.13

<P><B>ABSTRACT</B></P><P>Under the framework of an project ‘Building Asian Climate Change Scenarios by Multi‐Regional Climate Models Ensemble’, the ability of eight regional climate models and two fine‐resolution global climate models to reproduce late 20th century (1981–2000) precipitation climatology is assessed. Future precipitation change (2041–2060) under the A1B scenario is also quantified by applying four different ensemble methods: equal weighting, weighted mean (WM), reliability ensemble averaging (REA) and performance‐based ensemble averaging, after applying fourfold cross‐validation using observation and multi‐model‐simulated precipitation. The results indicate that the ensemble of simulated precipitation outperforms any single RCM in many aspects. Among the four ensemble approaches, the WM and REA methods show better skill in improving the simulation results, and are used for ensemble prediction of regional climate in Asia. Under the A1B scenario, the WM method estimates future precipitation change of approximately 0.2 mm day<SUP>−1</SUP> with less precipitation in northern and western China and northern India, and more precipitation in most other areas in Asia. The future annual precipitation will decrease by 0.1‐0.5 mm day<SUP>−1</SUP> in northern India, Pakistan and the central area of southern China. No significant change is found over eastern Kazakhstan, Mongolia, north‐central and western China.</P>

Visualizing Emerging Trends and International Cooperation Network in Pakistan’s Terrorism Research Literature

Youhong Lin,Shuyu Li,Bingfeng Fu,Ping Tang 한국국회학회 2019 한국과 세계 Vol.1 No.2

최근 몇 년간 국제 테러단체가 점점 확대고 있으며, 파키스탄은 새로운 테러단체인 “Arch of Instability” 의 중심이 되고 있다. 이는 파키스탄의 국내 정치 경제 발전에 심각한 위협을 줄 뿐만 아니라 국제 안보 구조와 대테러 국제정세에 심각한 위협을 가하고 있다. 또한 이는 중국 “일대일로”의 중요한 프로젝트인 “파키스탄 경제 통로”에도 심각한 위협이 되고 있다. 따라서 기존문헌을 정리한 결과를 파키스탄의 테러 상황에 대한 추가적인 연구가 필요하여 본 논문은 컴퓨터 과학 및 기술영역에서 새로 부각되고 있는 시각화 연구방법을 사용하여 Web of Science 데이터베이스를 활용하고 국제 학술계가 파키스탄의 테러에 대한 연구결과를 시각화 분석하여 국제학술계가 연구한 파키스탄 테러단체의 국가간의 협력망도 제출하였다. 더불어 파키스탄 테러 연구에 관한 국제 학술계의 저자 및 협력 네트워크에 대한 양적인 분석으로 파키스탄 테러 연구소의 국제 학술계의 연구 핫스팟 및 연구 트렌드도 정리하였다. In recent years, as the international terrorism is increasingly spread around, Pakistan has become one of the center of the Arch of Instability of the Indian subcontinent, which not only seriously threatens the political economy in Pakistan but also challenges the international safety pattern and anti-terrorist security situation severely. At the same time, this will also be a serious threat to the China-Pakistan Economic Corridor(CPEC), a flagship project of China s Belt and the Road Initiative(BRI). Therefore, it is necessary to study the current situation of Pakistan terrorism. The authors use the visualization research method to have visualization analysis of the results from international academe about the Pakistan terrorism research in database of Web of Science and depicts the international cooperation network of Pakistan terrorism studied by the international academe. The quantitative analysis of the international academe about the author and cooperation network of Pakistan terrorism research can help to further figure out the research hotspots and research fronts of the Pakistan terrorism research from the international academe.

Building Asian climate change scenario by multi‐regional climate models ensemble. Part I: surface air temperature

Tang, Jianping,Li, Qian,Wang, Shuyu,Lee, Dong‐,Kyou,Hui, Pinhong,Niu, Xiaorui,Gutowski Jr., William J.,Dairaku, Koji,McGregor, John,Katzfey, Jack,Gao, Xuejie,Wu, Jia,Hong, Song‐,You,Wang, Wiley 2016 International journal of climatology Vol.36 No.13

<P><B>ABSTRACT</B></P><P>Under the Asia‐Pacific Network for Global Change (APN) project ‘Building Asian Climate Change Scenarios by Multi‐Regional Climate Models Ensemble’ (RMIP III, Regional Model Intercomparision Project), the simulation results of eight regional climate models (RCMs) and two fine‐resolution global climate models are validated for reproducibility of the current surface air temperature climatology (1981–2000), and are used to generate surface air future temperature projections (2041–2060) over the CORDEX‐EA (A Coordinated Regional climate Downscaling Experiment‐East Asia) domain. Four ensemble methods, namely, the equal weighting, the weighted mean, the reliability ensemble averaging, and the performance‐based ensemble averaging, are employed to generate the multi‐model projection of regional climate change over the region. The results show that the regional temperature ensembles of the present climate obtained from all four methods can outperform a single RCM result in aspects of the spatial distribution as well as the seasonal variation over East Asia. The four ensemble methods are then used to project the regional temperature climatology under the IPCC emission scenario of A1B for 2041–2060. Compared with the control climate of 1981–2000, the annual mean temperature of the future climate (2041–2060) increases 1–2 °C in low latitude areas and 2–3 °C in middle–high latitude areas over Asia.</P>

Long short-term memory-based Malware classification method for information security

Kang, Jungho,Jang, Sejun,Li, Shuyu,Jeong, Young-Sik,Sung, Yunsick Elsevier 2019 Computers & electrical engineering Vol.77 No.-

<P><B>Abstract</B></P> <P>Signature-based malware detection approaches are inadequate for detecting the increasingly intelligent and large number of malware programs emerging today. Therefore, alternative approaches are required. The effects of malware can be estimated by analyzing the opcodes in its executable files. It can then be classified into families using a long short-term memory (LSTM) network. Vectorizing opcodes and application programming interface (API) function names using one-hot encoding results in high-dimensional vectors because each case is represented using one dimension. Therefore, this paper proposes a word2vec-based LSTM method to analyze opcodes and API function names using fewer dimensions. The results of opcode and API function name classification using the proposed method and one-hot encoding were compared using the Microsoft Malware Classification Challenge dataset. The proposed method showed approximately 0.5% higher performance than the one-hot encoding-based approach.</P>

The pivotal role of the NLRC4 inflammasome in neuroinflammation after intracerebral hemorrhage in rats

Gan Hui,Zhang Li,Chen Hui,Xiao Han,Wang Lu,Zhai Xuan,Jiang Ning,Liang Ping,Zheng Shuyue,Zhao Jing 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

The NLRC4 inflammasome, a member of the nucleotide-binding and oligomerization domain-like receptor (NLR) family, amplifies inflammation by facilitating the processing of caspase-1, interleukin (IL)–1β, and IL-18. We explored whether NLRC4 knockdown alleviated inflammatory injury following intracerebral hemorrhage (ICH). Furthermore, we investigated whether NLRC4 inflammasome activation can be adjusted by the regulator of G protein signaling 2/leucine-rich repeat kinase-2 pathway. Fifty microliters of arterial blood was drawn and injected into the basal ganglion to simulate the ICH model. NLRC4 small interfering RNAs (siRNAs) were utilized to knockdown NLRC4. An LRRK2 inhibitor (GNE7915) was injected into the abdominal cavity. Short hairpin (sh) RNA lentiviruses and lentiviruses containing RGS2 were designed and applied to knockdown and promote RGS2 expression. Neurological functions, brain edema, Western blot, enzyme-linked immunosorbent, hematoxylin and eosin staining, Nissl staining, immunoprecipitation, immunofluorescence assay and Evans blue dye extravasation and autofluorescence assay were evaluated. It was shown that the NLRC4 inflammasome was activated following ICH injury. NLRC4 knockdown extenuated neuronal death, damage to the blood-brain barrier, brain edema and neurological deficiency 3 days after ICH. NLRC4 knockdown reduced myeloperoxidase (MPO) cells as well as tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β and IL-18 following ICH. GNE7915 reduced pNLRC4 and NLRC4 inflammasome activation. RGS2 suppressed the interaction of LRRK2 and NLRC4 and NLRC4 inflammasome activation by regulating pLRRK2. Our study demonstrated that the NLRC4 inflammasome may aggravate the inflammatory injury induced by ICH and that RGS2/LRRK2 may relieve inflammatory injury by restraining NLRC4 inflammasome activation.

Pharmacokinetics/Pharmacodynamics models of veterinary antimicrobial agents

Wanhe Luo,Dongmei Chen,Mengru Wu,Zhenxia Li,Yanfei Tao,Qianying Liu,Yuanhu Pan,Wei Qu,Zonghui Yuan,Shuyu Xie 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.5

Misuse and abuse of veterinary antimicrobial agents have led to an alarming increase in bacterial resistance, clinical treatment failure, and drug residues. To address these problems, consistent and appropriate dosage regimens for veterinary antimicrobial agents are needed. Pharmacokinetics/Pharmacodynamics (PK/PD) models have been widely used to establish rational dosage regimens for veterinary antimicrobial agents that can achieve effective prevention and treatment of bacterial diseases and avoid the development of bacterial resistance. This review introduces building methods for PK/PD models and describes current PK/PD research progress toward rational dosage regimens for veterinary antimicrobial agents. Finally, the challenges and prospects of PK/PD models in the design of dosage regimens for veterinary antimicrobial agents are reviewed. This review will help to increase awareness of PK/PD modeling among veterinarians and hopefully promote its development and future use.

试论中印教育交流合作的理论与实践

和红,梅(Hongmei He),李蜀榆,(Shuyu Li),周雨婷,(Yuting Zhou) 한국국회학회 2020 한국과 세계 Vol.2 No.1

随着全球化的发展, 国家(区域)之间的交流合作日益得到推进。中印两国不仅是世界上人口最多的大国, 也是亚洲区域内最大的发展中国家, 加强两国之间政治、经济、社会文化等各领域的交流与合作不仅对亚洲乃至世界的和平与发展将做出突出的贡献。然而, 基于历史和现实原因, 中印两国之间的交流合作不论从深度还是广度方面都亟待得到提升。进入21世纪, 作为全球化影响的一种有效回应的跨国教育合作的发展日益成为中印交流合作的重要方面。然而, 由于历史遗留问题、政治互信问题、社会文化差异性问题、经济交流不充分等方面的问题导致中印交流合作表现出了不充分和不完善等方面的问题。2013年, “一带一路”倡议提出后, 以“包容性”著称的南亚大国印度却对此表现出“疑虑”和“消极”的态度。“文明因多样而交流, 因交流而互鉴, 因互鉴而发展”, 而“人是文明交流最好的载体”。因此, 在这个背景下, 中印两国更需要加强教育合作, 才能更好地实现两国文明的交流互鉴。 With the development of globalization, exchanges and cooperation between countries sub-regions have been promoted day by day. China and India are not only the most populous countries in the world, but also the largest developing countries in Asia. Strengthening exchanges and cooperation between the two countries in the political, economic, social and cultural fields will not only make outstanding contributions to peace and development in Asia and the world at large. However, due to historical and practical reasons, the exchanges and cooperation between China and India need to be improved in terms of both depth and breadth. In the 21st century, as an effective response to the impact of globalization, the development of transnational education cooperation has increasingly become an important aspect of Sino-Indian exchanges and cooperation. However, due to the problems left over from history, political mutual trust, social and cultural differences, inadequate economic exchanges and other problems, Sino-Indian exchanges and cooperation have shown inadequate and imperfect and other problems. In 2013, after the “BRI” put forward, India, a big country in South Asia known for its “inclusiveness”, showed a “skeptical” and “negative” attitude towards it. “Civilizations communicate because of diversity, learn from each other, and develop because of mutual learning”, while “people are the best carrier for civilization exchanges.” Therefore, in this context, China and India need to strengthen education cooperation in order to better realize the exchanges and mutual learning between the civilizations of the two countries.

Dark Soliton Solution of the Three-dimensional Gross-Pitaevskii Equation with an Isotropic Harmonic Potential and Nonlinearity in Polytropic Approximation

Xinwei Fan,Yu Zhou,Yalun Li,Ying Wang,Shuyu Zhou 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.3

We study the three-dimensional Fermi gas in an isotropic harmonic trap during the Bardeen- Cooper-Schrieffer superfluid to Bose-Einstein condensate (BCS-BEC) crossover, which is modeled by using the generalized Gross-Pitaevskii equation (GGPE) in the polytropic approximation. We analytically solved the 3D GGPE with a coupled modulus-phase transformation without introducing any additional integrability constraint, reaching the dark soliton-like solution. We find that the dark soliton identified undergoes an oscillation with a constant period over the whole BCS-BEC crossover region, although the amplitude of the dark soliton varies with polytropic index, demonstrating the peculiar nonlinear properties for the system modeled by using the 3D GGPE.