조선시대 권율 장군 모역 주변 계획 설계

김규섭,성백진,이재근 한국전통조경학회 2003 한국전통조경학회지 Vol.21 No.4

First, a strong theme had to be given to a grave park as a traditional space. The theme should increase recognition and concentration by the visitors to the site, attract their interest, and deliver a clearer meaning of the grave. Second, the design for the site and facility should be done in a way that the traditional theme could be expressed more effectively. The space for preservation, the space for participation and the space for tradition in the site are connected horizontally, rather than vertically so that the spaces could be utilized functionally and provide a natural landscape. Third, for the design of the plantation, trees and plants were selected in consideration of colors, appearances, textures, and images in order to harmonize with the properties of each space. Fourth, for the systematic management and operation, reasonable and systematic planning was applied, which constitutes the specialties of the grave park. The design was made in order to attract visitors through active advertisement and promotion by connecting with other traditional cultural resources of surrounding areas. And the guidance facilities were introduced to help understand the historical background of the grave, which has been regarded as hard to understand and boring.

Polyurethane의 혈전방지처리가 Staphylococcus epidermidis의 정착에 미치는 영향

김성민,이규백,백경란,오명돈,민병구,최강원 대한감염학회 1996 감염 Vol.28 No.1

목적 : 최근 생체재료로 만든 각종 카테타와 인공관절, 인공심장 등 인공장치의 사용이 점차 증가하고 있으며, 인공장치의 사용을 제한하는 가장 큰 합병증은 생체재료를 중심으로 일어나는 감염과 혈전증이다. 그러므로 인공장치를 장기간 사용하기 위해, 이들 합병증을 예방할 수 있는 생체재료를 개발하는 것이 중요한 과제가 되고 있다. 인공장치는 체내에 들어가서 혈액과 접촉하게 되면, 혈장단백질과 혈소판이 부착하고 활성화되어 혈소판-섬유소 혈전을 만든다. 이렇게 형성된 혈전은 세균의 부착을 촉진하고, 부착한 세균이 인체방어기전이나 항균제의 영향을 받지 않고 증식 할 수 있는 공간이 된다. 또한 정착한 세균은 혈액과 접촉하여, 혈전의 형성을 촉진한다. 즉, 혈전과 세균정착은 서로 영향을 주면서 인공자치의 오랜 사용을 제한하게 된다. 폴리우레탄에 설폰산과 알부민을 표면처리하면 혈전의 형성이 감소하는 효과가 있다는 것은 보고된 바 있으나, 혈전방지를 위해서 표면처리에 사용한 설폰산, 알부민이 세균 정착 및 감염에 미치는 영향에 대해서는 아직 잘 알려져 있지 않다. 이에 저자들은 설폰산, 알부민, 파라벤으로 처리한 폴리우레탄이 세균의 정착에 미치는 영향에 대해 알고자 본 연구를 시행하였다. 방법 : S. epidermidis RP12 부유액(?/mL)에 대조 폴리우레탄과 설폰산과 알부민, paraben으로 각각 처리한 폴리우레탄을 원반모양으로 만들어 담근 다음, 24시간 또는 48시간 동안 배양하였다. 세균 정착의 정도는 세균 집락수 및 SEM 소견으로 판정하였다. 결과 : 24시간 째 및 48시간 째의 세균집락수는 대조 폴리우레탄이 각각 8.3x10⁴/mL, 1.1×?/mL이었고, 파라벤 처리 폴리우레탄이 각각 9.3x10⁴/mL, 2.1x10⁴/mL (p<0.05), 설폰산 처리 폴리우레탄이 각각 1.2×?/mL, 8.2x10⁴/mL,(p<0.05), 알부민 처리 폴리우레탄이 각각 7.3×?/mL, 1,4×?/mL (p<0.05)이었다. 즉, 세균 집락수가 파라벤 처리 폴리우레탄에서는 유의하게 감소하였으나, 설폰산 처리 폴리우레탄에서는 증가하였다. SEM 소견에서는 설폰산 및 알부민 처리 폴리우레탄에서 세균 정착의 감소를 관찰하지는 못하였다. 결론: 본 연구는 in vitro 실험으로, 파라벤으로 처리한 폴리우레탄은 세균의 정착을 감소시키는 효과가 있었으나, 항혈전성 폴리우레탄은 세균 정착을 감소시키지 못하였다. 인체내에서는 세균과 혈소판, 혈장단백질이 복잡하게 반응을 하면서 혈전과 세균 정착이 서로 영향을 주므로, 인체내에서는 항혈전성 폴리우레탄이 혈전 형성을 감소시키고, 2차적으로 세균 정착을 감소시키고 감염을 예방하는 효과가 있을 가능성도 있으므로 이에 대한 연구가 진행되어야 하겠다. Background : Infection and thromboembolism are major complications of prolonged use of prosthetic devices, such as various catheters, prosthetic joints, and artificial hearts, frequently resulting in severe morbidity, or death. Therefore, the prevention of infection and thrombosis is an important subject in developing new biomaterials of prosthetic devices. The first event on insertion of a prosthetic device into the blood is protein adsorption and activation with platelet adhesion, activation and aggregation, leading to the formation of platelet-fibrin thrombi. The thrombi are susceptible to bacterial incorporation and protect the bacteria from the effects of circulating antibodies, phagocytic cells, and antibiotics. Furthermore, adherent bacteria may induce fibrin and platelet adhesion. Sulfonated polyurethane and albumin-immobilized polyurethane were documented to diminish the adhesion and shape-change of platelet on the polymer in comparison with plain polyurethane, in vitro. The objective of this study was to evaluate the effect of these antithrombotic biomaterials on the bacterial colonization. Methods : Plain, sulfonated, albumin-immobilized, and paraben-incorporated polyurethane(PU) films were prepared as a disk form. Staphylococcus epidermidis RP12 was suspended at a concentration of ?/mL and four types of polymeric disks were immersed in the bacterial suspension. After incubation for 24 and 48 hours, the bacterial colonization on the each disk was determined by the colony forming units(CFU) counting method. The colonized disks were examined by scanning EM. Results : The paraben decreased significantly the number of CFU on PU surface to 2.1x10⁴/mL after 48 hours, compared with 1.1×?/mL on plain polymers. The number of CFU on sulfonated or albumin-immobilized PU was 8.2x10⁴/mL, and 1.4×?/mL, respectively. SEM micrographs showed no reduction of colonization on antithrombotic PUs. Conclusion : Antithrombotic treatments of polymers did not decrease the bacterial colonization on polymers, in vitro, that was compared to the decreasing effect of paraben. However, because the thrombi and bacteria interact with each other, the overall effect of the antithrombotic polyurethanes on the bacterial colonization and infection should be studied in vivo.

기저세포암 및 편평세포암에서 미세혈관밀도와 p53 단백 발현에 관한 연구

어수락,조규성,안호범,김대영,이삼용,조백현 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Basal cell carcinoma(BCC) and squamous cell carcinoma (SCC) are very prevalent neoplasms of the human skin. Ultraviolet radiation in sunlight is a well-established mutagen of the p53 gene and is one of basal cell carcinoma and squamous cell carcinoma. The newly-formed vascular network is important for neoplasms to grow beyond a size of about 1 ㎣. Recent reports have suggested the hypothesis that a mutant p53 protein is closely related with capillary density. Immumohistochemistry for p53 protein and CD34 was performed in 20 cases of BCCs and 14 SCCs to evaluated the relationship between p53 protein and capillary density. The results were as follows:1. The microvessels stained by CD34 were mainly located in the interface of tumor cells and stroma. 2. There was no difference in the microvessel density according to the histologic types and age of the patients, but a higher microvessel density was noted in male patients. 3. The aggressive BCCs and the less-differentiated SCCs showed higher p53 immunostaining. 4. The mean microvessel density of cases showing strong positive immunostaining of the p53 gene(54.73±17.75) was higher than that of others(39.75±18.30). These results suggested that p53 protein expression and microvessel density are not related to the histologic types and age of the patients, but that differentiation and biologic behavior such as the infiltrating property of tumors and the microvessel density are closely related to p53 protein expression.

A Three-Dimensional and Sensitive Bioassay Based on Nanostructured Quartz Combined with Viral Nanoparticles

Lee, Jong-Hwan,Kim, Jung Suk,Park, Jin-Seung,Lee, Wonbae,Lee, Kyung Eun,Han, Sung-Sik,Lee, Kyu Back,Lee, Jeewon WILEY-VCH Verlag 2010 Advanced Functional Materials Vol.20 No.12

<P>An effective mask-free method for fabricating high-aspect-ratio pillarlike nanostructures over a large area of a quartz surface via a simple O<SUB>2</SUB> and CF<SUB>4</SUB> two-step reactive ion etching (RIE) procedure is developed. The nanostructured quartz surfaces are successfully combined with the engineered viral particles derived from hepatitis B virus capsid, yielding a novel 3D assay system with attomolar sensitivity, which has great potential for use in sensitive and early detection of various disease markers.</P> <B>Graphic Abstract</B> <P>Nanostructured quartz surfaces are densely covered with probe antibodies in a controlled orientation via engineered viral nanoparticles, leading to the preparation of a 3D assay system. The successful application of this nanostructured quartz-based assay system to highly sensitive detection of disease markers is reported. <img src='wiley_img_2010/1616301X-2010-20-12-ADFM200902054-content.gif' alt='wiley_img_2010/1616301X-2010-20-12-ADFM200902054-content'> </P>

Preparation of Ultra-High Purity Cylindrical Mo Ingot by Electron Beam Drip Melting

Lee, Back-Kyu,Oh, Jung-Min,Choi, Good-Sun,Rhee, Kang-In,Lee, Seoung-Won,Kim, Sang-Bae,Lim, Jae-Won The Japan Institute of Metals 2012 MATERIALS TRANSACTIONS Vol.53 No.2

<P>A cylindrical Mo ingot with ultra-high purity was obtained by electron beam (EB) drip melting process using a Mo rod. The Mo rod as a bar feeder was prepared from Mo powder by vacuum sintering and swaging processing. Most of impurities excluding W in the Mo ingot were removed down to below ppm level by three-times EB drip melting, where the purity of the Mo ingot except W was improved from the initial Mo powder purity of 3N (99.95%) to 5Nup (99.9998%). Furthermore, the gaseous impurities C, N and O in the Mo ingot were removed to a level below 5 ppm from the initial level of around 460 ppm by repetitive EB drip melting.</P>

Influence of oxygen concentration on mechanical properties of molybdenum powder during sintering.

Lee, Back-Kyu,Oh, Jung-Min,Shon, In-Jin,Kim, Hyung-Seok,Lim, Jae-Won American Scientific Publishers 2014 Journal of Nanoscience and Nanotechnology Vol.14 No.12

<P>In this study, the influence of oxygen concentration on the mechanical properties of sintered bodies produced from commercial and low-oxygen molybdenum powder via pulsed-current-activated sintering was determined. The hardness of the sintered bodies increased with the sintering temperature up to 1,500 degrees C and then decreased with further temperature increase. The hardness of the sintered low-oxygen-molybdenum body was slightly higher than the rest of the sintered bodies. This was because the relative density of the sintered low-oxygen-molybdenum body increased more than that of others as the sintering temperature increased. Furthermore, the grain size of the sintered commercial-molybdenum body was larger than that of the sintered low-oxygen-molybdenum body. This was attributed to the positive effect of molybdenum oxide on grain growth during sintering. Thus, it was established that low-oxygen molybdenum powder can suppress grain growth during sintering, resulting in improved mechanical properties of the sintered bodies.</P>

Biological Characterization of Marssonina coronaria Associated with Apple Blotch Disease

Lee, Dong-Hyuk,Back, Chang-Gi,Win, Nang Kyu Kyu,Choi, Kyung-Hee,Kim, Kyung-Min,Kang, In-Kyu,Choi, Cheol,Yoon, Tae-Myung,Uhm, Jae-Youl,Jung, Hee-Young The Korean Society of Mycology 2011 Mycobiology Vol.39 No.3

Marssonina coronaria associated with apple blotch disease causes severe premature defoliation, and is widely distributed in Korea. Thirteen isolates were collected from orchards located in Gyeongbuk Province from 2005~2007. All isolates displayed over 99.6% and 99.2% sequence similarity to each other in internal transcribed spacer regions and partial sequences of 28S rDNA, respectively. The isolates were phylogenetically closely related to Chinese isolates. Selected isolates did not differ in their pathogenicity. The optimum conditions for fungal growth were $20^{\circ}C$ and pH 6 on peptone potato dextrose agar (PPDA). Peptone and mannose were the best nitrogen and carbon source, respectively. Fungal growth was better on PPDA than on common potato dextrose agar. This study provides valuable information for integrated disease management program and facilitates the routine culturing of M. coronaria.

Arc 검출용 필터의 구현

이규민(Kyu-Min Lee),백원현(won-Hyen Back),이학범(Hack-Bum Lee),방선배(Sun-Bae Bang),박종연(chong-Yeon Pack) 대한전기학회 2011 대한전기학회 학술대회 논문집 Vol.2011 No.7

만성 이황화탄소 중독의 임상 양상

이규백(Kyu Back Lee),변현주(Hyun Ju Byoun),최태승(Tae Seung Choi),김선숙(Sun Sook Kim),조원용(Won Young Cho),김형규(Hyoung Kyu Kim),정태시(Tae See Chung) 대한내과학회 1990 대한내과학회지 Vol.39 No.2

N/A Carbon disulfide (CS2) is a volatile, colorless liquid and has an aromatic odor at room temperature. It has been used as a solvent for the spinning process in viscose rayon plants and also in cellophane and agricultural medicine plants, We experienced 38 workers who possibly suffered from chronic CS2, intoxication. Accordingly, we are reporting our opinions on synthetics and their effects on humanes. The time of exposure to CS2, gas was noted from 2 to 19years/(mean±SD 12±4.5yrs). The age range was from 30 to 64 years. With respect to clinical findings, patiets complained of neuralgia (86.8%), headache (63. 2%), memory disturbance (44.7%), visual dimness (36.8%), decreased sexual libido (29.6%), and hypertension (26.3%). The laboratory findings showed proteinuria in eight patients, azotemia in three patients, anemia in three patients and low HDL-cholesterol level in eight patients. Among the 38 workers, there was polyneuropathy in 13 patients by EMG, sensory neural hearing loss in nine patients, hemorrhage and exudate in eight patients by funduscopy, and cortical dysfunction in six patients by EEG.

Research Articles : Biological Characterization of Marssonina coronaria Associated with Apple Blotch Disease

( Dong Hyuk Lee ),( Chang Gi Back ),( Nang Kyu Kyu Win ),( Kyung Hee Choi ),( Kyung Min Kim ),( In Kyu Kang ),( Cheol Choi ),( Tae Myung Yoon ),( Jae Youl Uhm ),( Hee Young Jung ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.3

Marssonina coronaria associated with apple blotch disease causes severe premature defoliation, and is widely distributed in Korea. Thirteen isolates were collected from orchards located in Gyeongbuk Province from 2005~2007. All isolates displayed over 99.6% and 99.2% sequence similarity to each other in internal transcribed spacer regions and partial sequences of 28S rDNA, respectively. The isolates were phylogenetically closely related to Chinese isolates. Selected isolates did not differ in their pathogenicity. The optimum conditions for fungal growth were 20oC and pH 6 on peptone potato dextrose agar (PPDA). Peptone and mannose were the best nitrogen and carbon source, respectively. Fungal growth was better on PPDA than on common potato dextrose agar. This study provides valuable information for integrated disease management program and facilitates the routine culturing of M. coronaria.