Adrenomedullin inhibits IL-1관-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production.

Lee, Eun-Gyeong,Lee, Sang-Il,Chae, Han-Jung,Park, Seoung Ju,Lee, Yong Chul,Yoo, Wan-Hee Kluwer Academic/Plenum Publishers 2011 INFLAMMATION Vol.34 No.5

<P>To determine the effects of adrenomedullin (AM) on interleukin (IL)-1관-induced proliferation of rheumatoid synovial fibroblasts (RASFs) and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) and prostaglandin E2 (PGE2) by RASFs. The RASFs proliferation was evaluated with CCK-8 reagent in the presence of IL-1관 with/without AM (1-52) and AM inhibitor (AM (22-52)). MMPs, tissue inhibitor of metalloproteinase (TIMP-1), COXs, PGE2 and intracellular mitogen-activated protein kinase (MAPK) signalings, including p-ERK, p-p38, p-JNK were examined by immunoblotting or semiquantitative RT-PCR and ELISA. AM (1-52) inhibited IL-1관-induced RASFs proliferation and inhibited MMP-1, 3, COX-2 and PGE2 production. AM (1-52) also inhibited IL-1관-induced phosphorylation of ERK-1/2, p38, JNK. AM 22-52 inhibited the effects of AM (1-52) on proliferation of RASFs and production of MMP-1, 3, COX-2 via MAPKs. These results suggest that AM might involved joint destruction in rheumatoid arthritis and indicate that it might be a new therapeutic modality for management of this disease.</P>

Ethyl Acetate Fraction from <i>Cudrania Tricuspidata</i> Inhibits IL-1β-Stimulated Osteoclast Differentiation through Downregulation of MAPKs, c-Fos and NFATc1

Lee, Eun-Gyeong,Yun, Hee-Jin,Lee, Sang-Il,Yoo, Wan-Hee The Korean Association of Internal Medicine 2010 The Korean Journal of Internal Medicine Vol.25 No.1

<P><B>Background/Aims</B></P><P>The present study was performed to determine the effects of the ethyl acetate extract of <I>Cudrania tricuspidata</I> (EACT) on interleukin (IL)-1β-stimulated receptor activator of NF-κB ligand (RANKL)-mediated osteoclast differentiation.</P><P><B>Methods</B></P><P>Bone marrow cells were harvested from 6-week-old male imprinting control region mice, and the differentiation of osteoclasts from these cells was evaluated by tartrate-resistant acid phosphatase and resorption pit formation assay. Phosphorylated extracellular signal regulated kinase (p-ERK), phosphorylated p38, phosphorylated c-Jun amino-terminal kinase, NF-κB (p65), IκBα, c-Fos, and nuclear factor of activated T-cells c1 (NFATc1) expression was examined by immunoblotting and quantitative reverse transcription-polymerase chain reaction.</P><P><B>Results</B></P><P>EACT inhibits IL-1β-stimulated RANKL-mediated osteoclast differentiation. EACT also inhibits IL-1β-stimulated RANKL-mediated phosphorylation of ERK 1/2, p38 mitogen activated protein kinase, and expression of c-Fos and NFATc1.</P><P><B>Conclusions</B></P><P>These results suggest that EACT may be involved in the inhibition of bone loss by preventing osteoclast formation and may be used to manage bone destruction in inflammatory diseases, such as rheumatoid arthritis.</P>

Ethyl Acetate Fraction from Cudrania TricuspidataInhibits IL-1β-Stimulated Osteoclast Differentiation through Downregulation of MAPKs, c-Fos and NFATc1

( Eun Gyeong Lee ),( Hee Jin Yun ),( Sang Il Lee ),( Wan Hee Yoo ) 대한내과학회 2010 The Korean Journal of Internal Medicine Vol.25 No.1

Background/Aims: The present study was performed to determine the effects of the ethyl acetate extract of Cudrania tricuspidata (EACT) on interleukin (IL)-1β-stimulated receptor activator of NF-κB ligand (RANKL)-mediated osteoclast differentiation. Methods: Bone marrow cells were harvested from 6-week-old male imprinting control region mice, and the differentiation of osteoclasts from these cells was evaluated by tartrate-resistant acid phosphatase and resorption pit formation assay. Phosphorylated extracellular signal regulated kinase (p-ERK), phosphorylated p38, phosphorylated c-Jun amino-terminal kinase, NF-κB (p65), IκBα, c-Fos, and nuclear factor of activated T-cells c1 (NFATc1) expression was examined by immunoblotting and quantitative reverse transcription-polymerase chain reaction. Results: EACT inhibits IL-1β-stimulated RANKL-mediated osteoclast differentiation. EACT also inhibits IL-1β-stimulated RANKL-mediated phosphorylation of ERK 1/2, p38 mitogen activated protein kinase, and expression of c-Fos and NFATc1. Conclusions: These results suggest that EACT may be involved in the inhibition of bone loss by preventing osteoclast formation and may be used to manage bone destruction in inflammatory diseases, such as rheumatoid arthritis. (Korean J Intern Med 2010;25:93-100)

Probiotics가 대장암 세포주의 IL-8 생성에 미치는 영향

최창환 ( Choe Chang Hwan ),이상길 ( Lee Sang Gil ),양경민 ( Yang Gyeong Min ),채보아 ( Chae Bo A ),김태일 ( Kim Tae Il ),김원호 ( Kim Won Ho ) 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-

<목적> Probiotics는 살아있는 organism으로서 사람이 복용하였을 때 건강에 이로운 효과를 나타내는 것을 말하며, 최근 염증성장질환의 치료에 있어서 probiotics의 효과에 대해 많은 연구가 이루어지고 있다. 살아있는 비병원성 Salmonella 균주를 이용한 연구 보고에 의하면, 대장암 세포주에 염증성 자극 후 인산화된 I-κBα의 ubiquitination을 이 균주가 억제하여 염증반응을 억제한다고 하였다. 하지만 이러한 항염증 기전

결장암에 대한 활성 자연살해세포의 항암효능

성혜란(Hyeran Sung),김지연(Jee Youn Kim),박민경(Min Gyeong Park),김일회(Il-Hoi Kim),이동욱(Dong Wook Lee),한상배(Sang-Bae Han),이종길(Chong-Kil Lee),송석길(Sukgil Song) 대한약학회 2010 약학회지 Vol.54 No.3

Colorectal cancer is one of the most common alimentary malignancies. In this study, the antitumor activity of activated human natural killer (NK) cells against human colorectal cancer was evaluated in vivo. Human NK cells are the key contributors of innate immune response and the effective functions of these cells are enhanced by cytokines. Human peripheral blood mononuclear cells (PBMC) were cultured with interleukin-2 (IL-2)-containing medium for 14 days and resulted in enriched NK cell population. The resulting populations of the cells comprised 7% CD3+CD4+ cells, 25% CD3+CD8+ cells, 13% CD3-CD8+ cells, 4% CD3+CD16/CD56+ cells, 39% CD3+CD16/CD56- cells, and 52% CD3-CD16/CD56+ cells. Tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), IL-2, IL-4, and IL-5 transcripts of the activated NK cells were confirmed by RT-PCR. In addition, activated NK cells at doses of 2.5, 5 and 10 million cells per mouse inhibited 10%, 34% and 47% of SW620-induced tumor growth in nude mouse xenograft assays, respectively. This study suggests that NK cell-based immunotherapy may be used as an adoptive immunotherapy for colorectal cancer patients.

Association of Increased Pulmonary Interleukin-6 with the Priming Effect of Intra-Amniotic Lipopolysaccharide on Hyperoxic Lung Injury in a Rat Model of Bronchopulmonary Dysplasia

Kim, Do-Hyun,Choi, Chang Won,Kim, Ee-Kyung,Kim, Han-Suk,Kim, Beyong Il,Choi, Jung-Hwan,Lee, Myong Jin,Yang, Eun Gyeong S. Karger AG 2010 NEONATOLOGY Vol.98 No.1

<P><I>Background:</I> The authors previously demonstrated the priming effect of intra-amniotic lipopolysaccharide (LPS) on hyperoxic lung injury in a rat model of bronchopulmonary dysplasia (BPD). <I>Objectives:</I> To investigate the mechanism underlying this priming effect by determining biochemical profiles in a rat model of BPD. <I>Methods:</I> The rat model involved intra-amniotic LPS administration and postnatal hyperoxia (85%). The mRNA expressions of <I>interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), VEGF receptor-2 (VEGFR-2), basic fibroblast growth factor (bFGF),</I> and <I>transforming growth factor </I>β<SUB><I>1</I></SUB><I> (TGF-</I>β<SUB><I>1</I></SUB><I>),</I> as well as the protein levels of IL-6, VEGF, and protein carbonyl in lung tissue were compared between the LPS plus hyperoxia, the LPS only, the hyperoxia only, and the control groups. <I>Results:</I> Morphometric analysis of lung tissues demonstrated that alveolarization was significantly inhibited only in the LPS plus hyperoxia group. IL-6 protein levels and its mRNA expression in the lungs were significantly increased only in the LPS plus hyperoxia group. Neither LPS nor hyperoxia increased IL-6 in the lungs independently. <I>bFGF</I> mRNA expression was significantly decreased in the LPS-treated groups. VEGF protein levels were significantly reduced by hyperoxia, whereas protein carbonyl levels were increased by intra-amniotic LPS or hyperoxia. No additional significant change to VEGF or protein carbonyl levels was produced by intra-amniotic LPS or hyperoxia. There were no significant differences in the mRNA expressions of <I>VEGF, VEGFR-2,</I> and <I>TGF-</I>β<SUB><I>1</I></SUB>. <I>Conclusions:</I> The priming effect of intra-amniotic LPS on hyperoxic lung injury may be associated with IL-6 elevation in the lungs.</P><P>Copyright © 2009 S. Karger AG, Basel</P>

거대 점오염원이 주변 대기질에 미치는 영향에 관한 연구

김유근,이화운,전병일,장은숙,홍정혜,문윤섭,원경미,송정희 부산대학교 환경문제연구소 1996 環境硏究報 Vol.14 No.1

In order to show the effect of a vast point pollutant source on air quality of Pusan Thermoeletric Power Plant and its surrounding area, air quality around Pusan Thermoeletric Power Plant was simulated by ISCLT-2 which was supplied by EPA. For this purpose the emission amount of SO_2, NO_2 and TSP was calculated and atmospheric stability was classified for a recent decade(1985~1994) in Pusan. A result of the emission amount showed that much amount of NO_2, NO_2 and TSP are emitted from industrial area. It was clear that NO_2 is much emitted from line source and industrial area. And as a result of classification of atmospheric stability, neutral, stable and unstable state were 58%, 24.1% and 17.9%, respectivly. The result of ai quality simulation by ISCLT-2 showed that Pusan Thermoeletric Power Plant is affecting on the increse of 2.0ppb, 3.0ppb and 5.0㎍/㎥, SO_2, NO_2, and TSP respectively at its surrounding area, site A-3 which was located westward 2.2㎞ distance from Plant

Adrenomedullin inhibits MAPK pathway-dependent rheumatoid synovial fibroblast-mediated osteoclastogenesis by IL-1 and TNF-α

Yun, Hee-Jin,Lee, Eun-Gyeong,Lee, Sang-Il,Chae, Han-Jung,Yoo, Wan-Hee Springer-Verlag 2009 Rheumatology International Vol.29 No.10

65세 이상 외래 방문환자를 대상으로 한 심전도 소견

하원철,성낙일,김강,이경미,박준형,배준호,나득영 동국대학교 의학연구소 2008 東國醫學 Vol.15 No.1

The electrocardiogram is one of the most widely used diagnostic tools. Especially it is useful to the patients who have cardiovascular disease. Because elderly population are the fastest growing component of national population, I analyzed the frequencies of specific electrocardiogram findings in patients aged 65 years and older living in Gyeong-ju to collecting ECG data. Sinus bradycardia (17.0%) and Left ventricular hypertrophy (25.2%), QT prolongation (30.9%) were the most common abnormalities found in the population as a whole. Left-axis deviation (11.6% vs. 3.1%, p = 0.001) and right bundle branch block (11.9% vs. 4.1%, p= 0.001) were more common in men than women. First-atrioventricular block (2.2% vs. 5.5%, p = 0.025) and T wave abnormalities (4.1% vs. 13.2%, p = 0.001), left ventricular hypertrophy (21.6% vs. 28.0%, p = 0.049) were more common in women. QT prolongation were more common in 75 years and older (26.7% vs. 38.1%, p = 0.001). Left-axis deviation (9.4% vs. 3.9%, p = 0.024) and Right bundle branch blocks (9.8% vs. 3.0%, p = 0.003) were more common in men aged 65~74 years. Left ventricular hypertrophy (20.9% vs. 30.0%, p = 0.025) and T wave abnormalities (4.3% vs. 12.2%, p = 0.002) were more common in women aged 65~74 years. Atrial fibrillation (7.1% vs. 1.1%, p = 0.013) and left-axis deviation 17.6% vs. 2.1%, p = 0.001), Right bundle branch block (17.6% vs. 5.3%, p = 0.001) were more common in men aged 75 years and older. First-atrioventricular block (0% vs. 5.9%, p = 0.020) and T wave abnormalities (3.5% vs. 14.4%, p = 0.008) were more common in women aged 75 years and older. In conclusion, Left ventricular hypertrophy and sinus bradycardia, QT prolongation were most common electrocardiographic findings in as a whole. Left-axis deviation and right bundle branch block, fist-atrioventricular block, T wave abnormalities, QT prolongation were significantly different in age and gender. 심전도는 전 세계적으로 가장 널리 사용되는 진단적 도구로서, 특히 심혈관계 질환이 있는 환자에게 그 유용성이 크다. 현대 사회는 고령화 사회로 노인 인구에 대한 사회적, 의학적 관심이 높다. 이에 본 연구자는 노인 인구에 대한 심전도 자료를 수집하기 위해 동국대 경주 병원 외래 진료실을 방문한 65세 이상 노인 환자 737명을 대상으로 심전도 검사를 시행하였다. 좌심실 비대(25.2%), QT 간격 연장(30.9%)과 동성 서맥(17.0%)을 보인 경우가 가장 많았고, 좌축 편위 (11.6% vs. 3.1%, p = 0.001), 우각 차단(11.9% vs. 4.1%, p = 0.001)의 경우 여자 환자보다 남자 환자에서 빈도가 높았고, 1도 방실 차단(2.2% vs. 5.5%, p = 0.025)과 비정상적인 T파 양상(4.1% vs. 13.2%, p = 0.001), 좌심실 비대(21.6% vs. 28.0%, p = 0.049)의 경우 남자 환자보다 여자 환자에서 빈도가 높았다. 75세 이상 환자 군에서 QT 간격 연장(26.7% vs. 38.1%, p = 0.001)이 65세에서 74세 사이 환자 군에 비해 빈도가 높은 것 외에 유의한 차이는 없었다. 결론적으로 고령 환자에서 비정상적인 심전도 소견으로 좌심실 비대와 QT 간격 연장, 동성 서맥의 빈도가 높았으며 좌축 편위, 우각 차단, 1도 방실 차단 등은 성별, 연령대별로 유의한 차이가 있었다.

The Derived Neutrophil-to-Lymphocyte Ratio Is an Independent Prognostic Factor in Transplantation Ineligible Patients with Multiple Myeloma

Lee, Gyeong-Won,Park, Sung Woo,Go, Se-Il,Kim, Hoon-Gu,Kim, Min Kyoung,Min, Chang-Ki,Kwak, Jae-Yong,Bae, Sang-Byung,Yoon, Sung-Soo,Lee, Je-Jung,Kim, Ki Hwan,Nam, Seung-Hyun,Mun, Yeung-Chul,Kim, Hyo Jun S.Karger 2018 Acta haematologica Vol.140 No.3

<P><B><I>Background:</I></B> The neutrophil-to-lymphocyte ratio (NLR) is an independent prognostic marker in solid and hematological cancers. While the derived NLR (dNLR) was shown to be non-inferior to the NLR in large cohorts of patients with different cancer types, it has not been validated as a prognostic marker for multiple myeloma (MM) to date. <B><I>Methods:</I></B> Between May 22, 2011 and May 29, 2014, 176 patients with MM from 38 centers who were ineligible for autologous stem cell transplantation were analyzed. The dNLR was calculated using complete blood count differential data. The optimal dNLR cut-off value according to receiver operating characteristic analysis of overall survival (OS) was 1.51. All patients were treated with melphalan and prednisone combined with bortezomib. <B><I>Results:</I></B> The complete response rate was lower in the high dNLR group compared to the low dNLR group (7 vs. 26.1%, respectively; <I>p</I> = 0.0148); the corresponding 2-year OS rates were 72.2 and 84.7%, respectively (<I>p</I> = 0.0354). A high dNLR was an independent poor prognostic factor for OS (hazard ratio 2.217, 95% CI 1.015–4.842; <I>p</I> = 0.0458). <B><I>Conclusion:</I></B> The dNLR is a readily available and cheaply obtained parameter in clinical studies, and shows considerable potential as a new prognostic marker for transplantation-ineligible patients with MM.</P>