물 없이 사용하는 알코올 젤의 손소독 효과에 대한 연구

정재심,김덕희,김미나,최명애 대한기초간호자연과학회 2002 Journal of korean biological nursing science Vol.4 No.2

The purpose of this study was to compare the hand disinfection effect of waterless alcohol gel hand washing agent with that of soap and water, 4% chlorhexidine gluconate, and 10% povidone-iodine. Hands of fourty subjects were artificially contaminated with Acinetobacter baumannii 5㎖ and randomly distributed to each hand washing methods. Samples were collected from gloved hand by glove juice sampling procedure. Mean log reduction after hand washing were compared with baseline values. Number of microorganisms were converted to log and tested by ANOVA in SPSSWIN 100. Mean log reduction of soap and water, alcohol gel, 4% chlorhexidine gluconate, 10% povidone-iodine were 2.76±0.62, 2.97±0.56, 4.66±1.70, 4.60±0.91, respectively. The bactericidal effect of alcohol gel was similar to that of soap and water, but the effect was much less than chlorhexidine gluconate and povidone-iodine(p<0.001). In terms of microorganism reduction, the efficacy of waterless alcohol gel was almost the same as soap and water hand washing. Further evaluation of the bactericidal effect of waterless alcohol gel is needed because waterless alcohol gel is simple, convenient, and non-irritating hand washing agent and also very effective in busy hospital environment.

유산양 유방염에 대한 Chitosan 약침의 치료 효과

황수현,서진석,김덕환,김명철,신상태,전무형,南三郞,김영찬,이우근,표수일 충남대학교 수의과대학 동물의과학연구소 1999 動物醫科學硏究誌 Vol.7 No.-

To improve the therapeutic method for mastitis, treatment effect of chitosan pharmacopuncture was examined in three milking goats(A,B and C). Mastitis was artificially induced in two milking goats(A and B) and one goat(C) was naturally infected case with mastitis. The acupoint used was Yang-Ming and chitosan suspensions(provided from Tottory University, Japan: 30㎎ of chitosan/㎖)were injected to the acupoint with 5㎖/time for 3 days, respectively. As for the change of somatic cells in milk along with chitosan pharmacopuncture, they showed a decreasing tendency after increase on the 3rd day in all udders of B. In addition, as for the change of total leukocyte counts of peripheral blood with chitosan pharmacopuncture, they showed a decreasing tendency after increase on the 3rd day in B and C except A which showed a increasing tendency until on the 7th day. In the change of neutrophil/lymphocyte(N/L) ratio, increasing tendency was seen in A and C but increasing tendency after slight decrease was observed in B. Further, in the change of serum total protein and A/G ratio, serum total protein content was decreased after slightly increase on the 3rd day in A, and they were decreased after increase until on the 7th day in B and C, respectively. As for the change of A/G ratio, different pattern increasing tendency was shown in A, B and C.

항생제 대체제(앤타시드-100)의 급여가 젖소 송아지 육성에 미친 영향

명윤아,박덕섭,이인덕,남명수,이형석,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This study was carried out to develope an antibiotic substitute with some feed additive ingredients; activated charcoal, microbial products( Saccharomyces cerevisiae), sodium bentonite and pyroligneous. Sixty Holstein male calves(control 30 and tested 30 calves) were assigned to one of two diets, control(containing commercial antibiotic) and treatment diet (containing antibiotic substitute) with three replicates(10 calves each). The experiment were carried out for 30 days. The daily weight gains were similar between control(1.01kg/d) and treatment groups(1.01kg/d), however feed requirement were lower for treatment calves (2.80kg) than control calves (3.24kg) (P<0.05). Also calves were more health for treatment calves than control calves for diarrhea and respiratory diseases occurrences. The ammonia concentration of feces were lower for treatment calves(2.67 ppm) than for control calves(6.33 ppm) (P<0.05). No statistical difference were found in blood substances between control and treatment calves(P>0.05). In conclusion, the calve performances were improved by substitute without commercial antibiotic additive. 본 연구는 항생제를 대체할 목적으로 동물체의 활성을 가져오는 원료로 알려져 있는 활성탄과 목초액, sodium bentonite, 홍삼박, 생균제를 선별하여 이들 원료로 구성된 항생물질 대체제를 개발하여 홀스타인 수송아지에 급여하였을 때 생산성에 미치는 효과를 규명하고자 수행하였다. 송아지의 일당증체량은 대조구(1.011kg), 처리구(l.0lkg)간에 유의적인 차이가 없었고(P>0.05), 사료 요구율은 대조구 3.24에 비하여 처리구 2.80로 처리구에서 유의적인 개선효과를 나타냈다(P<0.05). 설사 발생률은 동일하였으나 분 상태는 처리구가 양호하였고, 호흡기 질병은 처리구에서는 발생하지 않았다. 분 중 NH₃ 농도는 대조구(6.33 ppm)에 비하여 처리구(2.67 ppm)에서 뚜렷한 개선 효과를 보였고(P<0.05), 혈액 수치는 대조구와 처리구가 유사하였다. 항생물질 대체제를 급여한 처리구가 시판 항생제 첨가사료급여군(대조구)에 비하여 증체에서는 다소 낮은 성적을 나타냈지만 질병 발생 없이 유사한 성적을 낸 것은 대체물질이 가축 소화기관의 활성화, 사료의 소화율 증진 등에 영향을 나타내어 동물의 활력이 증진되므로 항생제의 사용 없이도 양질의 축산물을 생산할 수 있는 결과를 얻었다.

활성탄의 급여가 젖소 송아지의 성장 및 배설물의 Ammonia 함량에 미치는 영향

유선일,박덕섭,명윤아,이인덕,남명수,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This study was carried out to investigate the influences of feed containing activated charcoal on the performances of calves The daily weight gain, feed intake, feed requirement and the content of ammonia in the excreta were measured. The experiment was conducted with 10 male calves for 30 days. Each were equally divided with treatment group and control group and for the treatment group's animal fed diets containing 1.0% activated charcoal. The daily weight gain of calves were decreased by 1.92% when they were fed activated charcoal(1.02㎏/day) compared to that of control calves(1.04㎏/day) and the daily feed intake of treatment group(2.86㎏/day) was 10.06% lower comparing to the control group(3.18㎏/day). In case of feed requirement, the result showed 8.2% lower in the treatment group(2.80㎏) than control group(3.06㎏). The content of ammonia in excreta was significantly lower for treatment group(0.33ppm) than control group (2.67ppm)(p<0.05), which showed more improvement for reducing ammonia content in the treatment group with 87.6%(p<0.05). 본 연구는 사료에 활성탄을 첨가했을 때 송아지의 일당 증체량, 사료 섭취량, 사료 요구율 및 배설물의 ammonia 함량에 미치는 영향을 규명하기위해 실시하였다. 홀스타인 수송아지 10두를 30일 동안 대조구, 시험구(활성탄 1%첨가)로 각5두 공시하여 시험하였으며, 실험 결과를 요약하면 다음과 같다. 송아지의 일당 증체량은 대조구(1.04kg/일)에 비하여 활성탄을 급여했을 때(l.02kg/일) 1.92% 감소하였고, 일당 사료 섭취량은 시험구(2.86kg/일)가 대조구(3.18kg/일)에 비해서 10.06% 낮았으나, 사료 요구율은 대조구(3.06kg/kg증체)에 비해 시험구(2.80kg/kg증체)에서 8.2% 낮은 결과를 나타내었다. 송아지 배설물의 ammonia 함량은 대조구의 2.67ppm에 비하여 시험구가 0.33ppm으로 87.6% 감소되는 개선효과를 보였다(p<0.05).

보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

Outcome of Multipair Donor Kidney Exchange by a Web-Based Algorithm

Kim, Beom Seok,Kim, Yu Seun,Kim, Soon Il,Kim, Myoung Soo,Lee, Ho Yung,Kim, Yong-Lim,Kim, Chan Duck,Yang, Chul Woo,Choi, Bum Soon,Han, Duck Jong,Kim, Yon Su,Kim, Sung Joo,Oh, Ha-Young,Kim, Dae Joong American Society of Nephrology 2007 Journal of the American Society of Nephrology Vol.18 No.3

<P>Donor kidney exchange is an established method to overcome incompatibility of donor-recipient pairs (DRP). A computerized algorithm was devised to exchange donor kidney and was tested in a multicenter setting. The algorithm was made according to the consensus of participating centers. It makes all possible exchange combinations not only between two incompatible DRP but also circularly among three DRP and selects an optimum set of exchange combinations, considering several factors that can affect the outcome of the exchanged transplant. The algorithm was implemented as a web-based program, and matching was performed five times. Fifty-three DRP were enrolled from five transplant centers. The numbers of DRP that were enrolled in each matching were 38 (25:13), 39 (34:5), 33 (31:2), 32 (28:4), and 34 (30:4) (carryover:newcomer). The numbers of generated exchange combinations were 4:11, 3:17, 2:12, 2:3, and 2:3 (two-pair exchange:three-pair exchange), and the numbers of DRP in selected exchange combinations were six, 12, six, five, and four in each matching. The numbers of DRP with blood type O recipient or AB donor were five and one, respectively, in selected exchange combinations. Six DRP of two-pair exchange combinations and six DRP of three-pair exchange combinations underwent transplantation successfully. Computerized algorithm of donor kidney exchange was tried not only between two incompatible DRP but also circularly among three DRP. It showed that the algorithm has potential to improve the outcome of donor kidney exchange, especially for disadvantaged DRP with blood type O recipients or AB donors.</P>

넙치, Paralichthys olivaceus에 투여된 Edwardsiella tarda와 Streptococcus iniae에 대한 혼합백신의 예방효과와 성장에 미치는 영향

김명석 ( Myoung Sug Kim ),김경덕 ( Kyoung Duck Kim ),김강웅 ( Kang Woong Kim ),박명애 ( Myoung Ae Park ),김진우 ( Jin Woo Kim ) 한국어병학회 2009 한국어병학회지 Vol.22 No.3

Study on the ZnO photocatalytic activities by hydrogen treatment and annealing treatment

Myoung-hwa Kim,Sang-Hun Nam,Sang Duck Lee,Kang-Suk Lee,Young Dok Kim,Jin-Hyo Boo 한국진공학회 2009 한국진공학회 학술발표회초록집 Vol.37 No.-

Enhanced tolerance to methyl viologen-induced oxidative stress and high temperature in transgenic potato plants overexpressing the <i>CuZnSOD</i>, <i>APX</i> and <i>NDPK2</i> genes

Kim, Myoung Duck,Kim, Yun-Hee,Kwon, Suk-Yoon,Yun, Dae-Jin,Kwak, Sang-Soo,Lee, Haeng-Soon Blackwell Publishing Ltd 2010 Physiologia plantarum Vol.140 No.2

<P>Oxidative stress is a major threat for plants exposed to various environmental stresses. Previous studies found that transgenic potato plants expressing both copper zinc superoxide dismutase (<I>CuZnSOD</I>) and ascorbate peroxidase (<I>APX</I>) (referred to as SSA plants), or nucleoside diphosphate kinase 2 (<I>NDPK2</I>) (SN plants), showed enhanced tolerance to methyl viologen (MV)-induced oxidative stress and high temperature. This study aimed to develop transgenic plants that were more tolerant of oxidative stress by introducing the <I>NDPK2</I> gene into SSA potato plants under the control of an oxidative stress-inducible peroxidase (<I>SWPA2</I>) promoter to create SSAN plants. SSAN leaf discs and whole plants showed enhanced tolerance to MV, as compared to SSA, SN or non-transgenic (NT) plants. SSAN plants sprayed with 400 µ<I>M</I> MV exhibited about 53 and 83% less visible damage than did SSA and SN plants, respectively. The expression levels of the <I>CuZnSOD</I>, <I>APX</I> and <I>NDPK2</I> genes in SSAN plants following MV treatment correlated well with MV tolerance. SOD, APX, NDPK and catalase antioxidant enzyme activities were also increased in MV-treated SSAN plants. In addition, SSAN plants were more tolerant to high temperature stress at 42°C, exhibiting a 6.2% reduction in photosynthetic activity as compared to plants grown at 25°C. In contrast, the photosynthetic activities of SN and SSA plants decreased by 50 and 18%, respectively. These results indicate that the simultaneous overexpression of <I>CuZnSOD, APX</I> and <I>NDPK2</I> is more effective than single or double transgene expression for developing plants with enhanced tolerance to various environmental stresses.</P>

애기장대(Arabidopsis thaliana)의 잎과 줄기 절편체로부터 기관분화를 통한 식물체 재분화

Myoung Duck KIM,Miyong Chang,Joon Chul KIM 강원대학교 기초과학연구소 2000 기초과학연구 Vol.11 No.-

Shoot induction from leaf and stern explants of Arabidopsis tfr1liana was obtained when leaf explants were cultured on MS medium containing 0.15 mg/L lAA and 5 mg/L 2-jp after liquid preculture on MS medium containing 5 mg/L lAA and 0.5 mg/L 2,4- D for 7 days and also, when stem explants were cultured on MS medium containing 0.15 mg/L lAA and 5 mg/L 2-jp after liquid preculture on CP medium containing 0.5 mg/L 2,4-D and 0.1 mg/L BAP for 7 days. Callus induction from stern explants was obtained on CP medium containing 0.5 mg/L 2,4-D and 0.1 mg/L BAP in the dark at 25 t: after four weeks of culture. Shoot forming calli selected from subcultured-calli were cultured on MS medium containing 0.05 mg/L lAA and 7 mg/L 2-jp under continous illumination for 4 weeks. Shoot regenerating frequency (explants and calli regenerating at least one shoot) was more than 55%. Regenerated shoots were rooted on MS medium without phytohomones and transplanted to potting soil. Histological observations of leaf and stern explants revealed that meristematic dome was originated from massive cells of epidermal and subepidermal cells and that meristemoid from the shoot forming calli developed to shoot primordia and shoot.