Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.

영상융합방법에 의한 거리측정

김지영,이희만 서원대학교 응용과학연구소 2000 응용과학연구 Vol.9 No.1

A stereo fusion method for measuring the distance from the multiple cameras to the target is proposed. the Parallel Looking Camera Model is employed and analyzed in this paper. The peak-valley shape feature is extracted from the gray level waveform on the epipolar line of the image plane. The matching process uses not only the said peak-valley information but also the gray level intensity of the local peak-valley waveform on the candidate waveforms. The left image is used as a reference for registering the right image. The right image is warpped for the said registration. The fused image which is a overlapped image of the reference left and the warpped right image is used to calculate RMSE(Root Mean Square Error). The fusion process is ended when the calculated RMSE value reaches its minimum level or the bottom threshold. The distance is obtained during the warping process by using the Parallel Looking Camera Model parameters.

영상처리에 의한 버스전용차로 위반차량의 검출방법

김지영 서원대학교 응용과학연구소 2001 응용과학연구 Vol.10 No.1

In this paper, an image processing method detecting the vehicles which violate the exclusive bus lain traffic regulation is proposed. The tow cameras are used to capture the images of the first lain of a highway where the exclusive bus lain traffic regulation is enforced. One camera is used to capture the front view of a vehicle, and the second camera to capture the side view of the craft. The front view camera is used for recognizing the vehicle licence number. The side view camera is employed for categorizing the vehicle class. The proposed method in this paper is very practical to be implemented because it does not need any attachment on the vehicle such as an identification transmitter or any other electronic devices.

점포내 VMD요소가 SPA 브랜드 정체성에 미치는 영향

김소영, 손지훈, 심효진, 신동훈, 정일호, 홍동우, 김은영 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

This study was to examine the effect of VMD factors on brand identity for SPA brands. As brand stimulus, four SPA brands(e.g., Basic House, Codes combine, Uniqlo, Zara) were selected by pretest. For collecting data, a self-administered questionnaire was developed based on the literatures and intercept survey was conducted. A total 508 usable responses were obtained from consumers who shopped at the selected brands' stores. For data analysis, descriptive statistics, factor analysis, and regression analysis were used. As perceived by consumers, VMD consisted of seven factors: lightening, scent, color, layout, music, window display, and item presentation. The VMD factor was significantly related to brand identity for the SPA brands. Specifically, brand awareness was predicted by color, layout and item presentation; brand association strength was predicted by color, layout, and widow display; favorable brand association was predicted by item presentation; salience of brand association was predicted by color and music; and brand image association was predicted by color, layout, music, and item presentation. Thus, this study discussed a managerial implication for SPA retailers to develop a visual merchandising strategy in the competitive marketplace.

신경인성 방광 1례에 대한 임상고찰

김만호,이지영,이정원,조충식,김철중 대전대학교 한방병원 2002 惠和醫學 Vol.11 No.1

From January 10th 2002 to February 17th 2002, the clinical study was carried out I case of patient in trouble with voiding difficulty such as urinary incontinence, enuresis and urinary frequency after the L₄-L_(5) HN P operation, who had been hospitalised in the Dept. of Internal Medicin e, Cheon-An Oriental Medical Hospital, Daejeon University. The results were as following 1. For this case, we could get the better result using Bo-Joong-lk-Gi-Tang(補中益氣湯) in the treating period. 2. We could get the effective result that the case of voiding difficulty after HNP operation and cerebral infarction by conservative therapy of oriental medicine such as acupuncture, moxibustion and herb medicine.

근피로 후 세 가지 회복 방법

이예영,김지은,서예은,정다연,강현주 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Muscle fatigue is a symptom that decreases your muscles’ ability to perform over time. It can be associated with a state of exhaustion, often following excessive sports activity or exercise. While exercise is a common cause of muscle fatigue, this symptom can be the result of other health conditions. Muscle fatigue caused by excessive activity limits activities of daily living and difficult to maintain a stable posture. Therefore, the purpose of this study was to investigate the effect of stretching, icing, and sports massage on muscle fatigue recovery.

석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율

임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.

자유연애의 이상과 식민지 근대의 현실 : 김동인의 「약한 자의 슬픔」 연구

김지영 우리어문학회 2003 우리어문연구 Vol.20 No.-

The acclamation for free marriage and free love was an important constituent of the process of attempting to construct the institutional emotion and family structure newly modeling the modern western romantic love. Until the late 1910s, the idea of free marriage and free love was too strongly tied with the ideology of enlightenment, thus only part of the meaning, the freedom to choose a marital partner, was stressed. Moreover, marriage was strongly tied up with the will to devote toward the nation, so choosing a marital partner was on the will to be a good people for the nation rather than on natural feeling of love. Free love started to be described in its genuine meaning from the works of Kim Tongin and his colleagues of the literary magazine "Creation", though the meaning of it was still unstable and on the way of changing. "The Sorrow of the Weak" showed the inauthenticity of the concept of free love by describing a woman's inner inconsistence and the ordeals that happened in her life while she aspired to romantic love. Kim, the author, showed the leftovers of pre-modern ethic inside a woman of the time who was seeking modern life and revealed the abstractness of her admiration for romantic love. We can find the contradiction between pre-modern and modern love ethics and the conflict between physical and spiritual love in the protagonist's interior. The falsehood, disorganization, and inversion of love revealed by Elizabeth while she was chasing after romantic love and modern life were not different from the falsehood, disorganization, and inversion of the idea of free love of the period itself. This reflects one of the colonial characteristics of Korean modernization that was considered as the most urgent and transcendental aim by many Korean enlighteners of the day.

여수국가산업단지 인근 지역 대기오염물질 농도에 영향을 미치는 요인분석

김민영,변정훈,이영훈,이건영,한지은,이예림,임지현,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Eight monitoring stations in the Yeosu National Industrial Complex will determine the concentration of air pollutants measured every hour in 2019, analyze what factors affect them, and how they correlate with the air pollutant measurements. Six air pollutants (SO2, NO2, O3, CO, PM-10 and PM-2.5) measured at eight monitoring stations around Yeosu National Industrial Complex (Seogang-dong, Wolnae-dong, Munsu-dong, Yeocheon-dong, Deokchung-dong, Hwayang-myeon, Yulchon-myeon, Samil-dong) the measurement data for each hour of the year were downloaded to analyze the concentration by weather condition, time zone, season, separation distance from Yeosu Industrial Complex, and separation distance from Gwangyang Industrial Complex. SO2, NO2, and CO concentrations were significantly higher in winter, significantly lower in summer, and O3, PM-10, and PM-2.5 concentrations were significantly higher in spring. SO2, CO, PM-10, and PM-2.5 concentrations were significantly higher between 10 a.m. and 12 p.m., NO2 concentrations were significantly higher between 7 a.m. and 9 a.m., and O3 concentrations were significantly higher between 13 p.m. and 15 p.m.(p<0.001). The concentration of air pollutants (SO2, NO2, CO, PM-10, PM-2.5) excluding O3 was found to have a negative (-) correlation with weather condition, and in the case of O3, it was found to have a positive (+) correlation with PM-10, PM-2.5, temperature and wind speed. It was confirmed that the concentration of air pollutants emitted from the Yeosu National Industrial Complex differs depending on the season, time and distance to the industrial complex. This study aims to establish measures to minimize health damage to residents living relatively close to the industrial complex and contribute to the establishment of management and response measures according to the properties of substances to reduce the concentration of air pollutants in the future.